Objective:To proliferation in patients with liver cancer.Methods: Patients with primary liver cancer who underwent surgical resection in Yulin Third Hospital between December 2013 and December 2016 were selected as th...Objective:To proliferation in patients with liver cancer.Methods: Patients with primary liver cancer who underwent surgical resection in Yulin Third Hospital between December 2013 and December 2016 were selected as the liver cancer group of the research, and healthy volunteers who received physical examination in Yulin Third Hospital over the same period were selected as the control group of the research. Serum was collected from both groups to test the contents of ASPH and 5'-NT as well as liver cancer cell proliferation molecules;liver cancer lesions and para-carcinoma lesions were collected from liver cancer group to detect the contents of angiogenesis molecules and cancer cell proliferation molecules.Results: Serum ASPH and 5'-NT contents in liver cancer group were significantly higher than those in control group;VEGF, VEGFR1, VEGFR2, HGF, EGFR, Bcl-2, Bcl-x and Bcl-w contents in liver cancer lesions were significantly higher than those in para-carcinoma lesions and positively correlated with serum ASPH and 5'-NT contents in patients with liver cancer while Bax and Bak contents in liver cancer lesions were significantly lower than those in para-carcinoma lesions and negatively correlated with serum ASPH and 5'-NT contents in patients with liver cancer;serum AFP, GP73, GPC3 and DKK1 contents in liver cancer group were significantly higher than those in control group and positively correlated with serum ASPH and 5'-NT contents in patients with liver cancer.Conclusion: Serum ASPH and 5'-NT contents increase significantly in patients with liver cancer and can accurately evaluate angiogenesis and cancer cell proliferation.展开更多
目的建立一种高效液相色谱法同时测定四物汤中5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸四种活性成分的含量。方法采用Phenomenex luna C18(250mm×4.6mm,5.0μm)色谱柱,流动相:乙腈(A)-0.05%甲酸水溶液(B),线性梯度洗脱(0~5min,A相5%;...目的建立一种高效液相色谱法同时测定四物汤中5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸四种活性成分的含量。方法采用Phenomenex luna C18(250mm×4.6mm,5.0μm)色谱柱,流动相:乙腈(A)-0.05%甲酸水溶液(B),线性梯度洗脱(0~5min,A相5%;5~10min,A相5%→10%;10~20min,A相10%→20%;20~40min,A相20%→30%;40→46min,A相30%→5%);流速:1.0mL·min^(-1);检测波长:230nm和282nm。柱温:35℃。结果 5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸分别在0.0215~0.43mg·mL^(-1)、0.021~0.42mg·mL^(-1)、0.0195~0.39mg·mL^(-1)、0.023~0.46mg·mL^(-1)范围内呈良好线性关系。四物汤中5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸四种有效成分的含量分别为0.0681%、未检出、0.250%、0.0358%。结论该方法操作简便,结果准确可靠。展开更多
文摘Objective:To proliferation in patients with liver cancer.Methods: Patients with primary liver cancer who underwent surgical resection in Yulin Third Hospital between December 2013 and December 2016 were selected as the liver cancer group of the research, and healthy volunteers who received physical examination in Yulin Third Hospital over the same period were selected as the control group of the research. Serum was collected from both groups to test the contents of ASPH and 5'-NT as well as liver cancer cell proliferation molecules;liver cancer lesions and para-carcinoma lesions were collected from liver cancer group to detect the contents of angiogenesis molecules and cancer cell proliferation molecules.Results: Serum ASPH and 5'-NT contents in liver cancer group were significantly higher than those in control group;VEGF, VEGFR1, VEGFR2, HGF, EGFR, Bcl-2, Bcl-x and Bcl-w contents in liver cancer lesions were significantly higher than those in para-carcinoma lesions and positively correlated with serum ASPH and 5'-NT contents in patients with liver cancer while Bax and Bak contents in liver cancer lesions were significantly lower than those in para-carcinoma lesions and negatively correlated with serum ASPH and 5'-NT contents in patients with liver cancer;serum AFP, GP73, GPC3 and DKK1 contents in liver cancer group were significantly higher than those in control group and positively correlated with serum ASPH and 5'-NT contents in patients with liver cancer.Conclusion: Serum ASPH and 5'-NT contents increase significantly in patients with liver cancer and can accurately evaluate angiogenesis and cancer cell proliferation.
文摘目的建立一种高效液相色谱法同时测定四物汤中5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸四种活性成分的含量。方法采用Phenomenex luna C18(250mm×4.6mm,5.0μm)色谱柱,流动相:乙腈(A)-0.05%甲酸水溶液(B),线性梯度洗脱(0~5min,A相5%;5~10min,A相5%→10%;10~20min,A相10%→20%;20~40min,A相20%→30%;40→46min,A相30%→5%);流速:1.0mL·min^(-1);检测波长:230nm和282nm。柱温:35℃。结果 5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸分别在0.0215~0.43mg·mL^(-1)、0.021~0.42mg·mL^(-1)、0.0195~0.39mg·mL^(-1)、0.023~0.46mg·mL^(-1)范围内呈良好线性关系。四物汤中5-羟甲基糠醛、川芎嗪、芍药苷、阿魏酸四种有效成分的含量分别为0.0681%、未检出、0.250%、0.0358%。结论该方法操作简便,结果准确可靠。