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温胃阳汤对功能性消化不良大鼠肥大细胞活化及SCF/c-Kit信号通路的影响 被引量:1
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作者 税典奎 黎舒婷 +4 位作者 黄慧花 龙海华 杨健 罗诗雨 覃凌娜 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第1期74-80,共7页
目的:基于肥大细胞活化及干细胞因子(stem cell factor,SCF)/受体酪氨酸激酶c-Kit信号通路探讨温胃阳汤治疗大鼠功能性消化不良的作用机制。方法:将60只SD大鼠随机分为空白组,模型组,雷尼替丁组及温胃阳汤低、中、高剂量组,每组10只。... 目的:基于肥大细胞活化及干细胞因子(stem cell factor,SCF)/受体酪氨酸激酶c-Kit信号通路探讨温胃阳汤治疗大鼠功能性消化不良的作用机制。方法:将60只SD大鼠随机分为空白组,模型组,雷尼替丁组及温胃阳汤低、中、高剂量组,每组10只。空白组大鼠不予造模,其他各组采用夹尾刺激加不规则喂养复合番泻叶法建立大鼠功能性消化不良模型,模型建立后,空白组及模型组灌胃给予生理盐水,温胃阳汤低、中、高剂量组和雷尼替丁组则分别用温胃阳汤(0.743 g/mL、1.485 g/mL和2.970 g/mL)及盐酸雷尼替丁胶囊(3 g/L)灌胃。治疗结束后,以碳墨推进法测定小肠推进率;采用甲苯胺蓝染色观察大鼠十二指肠组织肥大细胞并计数;ELISA测定大鼠十二指肠中肥大细胞类胰蛋白酶(mast cell tryptase,MCT)和组胺(histamine,HA)的含量;RT-qPCR检测十二指肠中SCF和c-Kit mRNA的表达;Western blot和免疫组化检测十二指肠中SCF和c-Kit蛋白的表达水平。结果:与模型组相比,温胃阳汤治疗显著提高大鼠的小肠推进率(P<0.05);ELISA结果显示,温胃阳汤治疗可减少大鼠十二指肠黏膜组织肥大细胞数量及MCT和HA含量(P<0.05);Western blot和免疫组化结果表明,温胃阳汤治疗可上调大鼠十二指肠组织c-Kit和SCF蛋白的表达水平(P<0.05),增加SCF和c-Kit阳性细胞数(P<0.05);RT-qPCR结果显示,WWYD治疗可上调大鼠十二指肠组织c-Kit和SCF mRNA的表达(P<0.01)。而且,小肠推进率分别与MCT和HA含量呈负相关,与SCF和c-Kit的表达呈正相关。结论:温胃阳汤能促进大鼠十二指肠动力,其作用机制可能与抑制大鼠十二指肠MCT和HA的生成,及激活SCF/c-Kit信号通路有关。 展开更多
关键词 功能性消化不良 温胃阳汤 肥大细胞 scf/c-Kit信号通路 十二指肠
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SCF基因参与哺乳动物肤色和毛色表型形成的研究进展
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作者 陈旭 刘欣 邢秀梅 《黑龙江畜牧兽医》 CAS 北大核心 2024年第2期28-32,42,共6页
毛色作为动物重要表型之一一直是人们的研究热点。干细胞因子(stem cell factor, SCF)基因参与动物细胞发育(包括毛色形成),在启动子水平调节黑色素细胞特异性小眼转录因子(protein and melanocyte-specific microphthalmia-related tra... 毛色作为动物重要表型之一一直是人们的研究热点。干细胞因子(stem cell factor, SCF)基因参与动物细胞发育(包括毛色形成),在启动子水平调节黑色素细胞特异性小眼转录因子(protein and melanocyte-specific microphthalmia-related transcription factor, MITF)的mRNA表达,且在不同物种中参与机体内外环境刺激(主要是紫外线辐射)导致的色素沉着过程,在黑色素细胞迁移和存活、斑点(roan)表型及某些疾病中起重要作用。笔者对SCF基因的结构性质及在哺乳动物黑色素合成及肤色和毛色形成中的作用机制研究进展进行综述,旨在为进一步探究SCF基因的分子机制提供思路和见解。 展开更多
关键词 毛色性状 毛色形成 scf基因 MITF基因 roan表型 异构体
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基于SCF/C-kit信号通路探讨益髓破血方改善脑出血小鼠肠动力“通腑”作用机制
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作者 廖颖 李萍 《吉林中医药》 2024年第9期1075-1079,共5页
目的观察益髓破血方对脑出血小鼠排便情况与结肠组织干细胞因子(stem cell factor,SCF)/酪氨酸激酶受体(kit proto-oncogene,C-kit)信号通路的影响,探讨益髓破血方调节脑出血小鼠肠动力通腑作用机制。方法将54只SPF级健康雄性C57BL/6J... 目的观察益髓破血方对脑出血小鼠排便情况与结肠组织干细胞因子(stem cell factor,SCF)/酪氨酸激酶受体(kit proto-oncogene,C-kit)信号通路的影响,探讨益髓破血方调节脑出血小鼠肠动力通腑作用机制。方法将54只SPF级健康雄性C57BL/6J小鼠随机均分为假手术组、模型组与方剂治疗组,采用鼠尾自体血注入法制作实验性脑出血模型,假手术组只进针不注射。方剂治疗组给予1 g/mL益髓破血方悬浊液灌胃,假手术组、模型组给予等体积生理盐水灌胃,每天1次。观察各组小鼠排便情况,记录第1天、第3天、第7天同时段6 h内的排便量以及末次灌胃后首次排便间隔时间,并于相同时间分段点处死小鼠取材,采用免疫组化和Western Blot法检测小鼠结肠组织中的SCF、C-kit蛋白。结果与假手术组比较,模型组小鼠第1天、第3天、第7天各6 h内排便量显著减少(P<0.05),末次灌胃首次排便时间明显滞后(P<0.05),结肠组织中SCF、C-kit蛋白表达显著减少(P<0.05),且随周期延长呈逐渐降低趋势;与模型组比较,方剂治疗组第1天、第3天、第7天各6 h内排便量相对增多(P<0.05),首次排便时间缩短(P<0.05),SCF、C-kit蛋白表达水平升高(P<0.05),且随时间推移逐渐向正常量恢复。结论益髓破血方干预治疗脑出血小鼠,可以增加肠动力,促进排便,发挥通腑作用使肠功能恢复,改善脑出血后便秘,其机制可能与SCF/C-kit信号通路有关。 展开更多
关键词 脑出血 益髓破血方 scf/C-kit信号通路 肠动力 通腑
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基于SCF/c-kit信号通路探讨理气通便方对气滞型慢传输型便秘大鼠肠道动力的影响
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作者 柯丹枫 刘启鸿 +6 位作者 骆云丰 柯晓 胡露楠 严锦贤 任彦 方文怡 赵培琳 《福建中医药》 2024年第2期12-16,共5页
目的 基于SCF/c-kit信号通路探讨理气通便方对气滞型慢传输型便秘(STC)大鼠肠道动力的影响。方法 将36只Wistar雌性大鼠按随机数字表法分为对照组6只和造模组30只。造模组采用“洛哌丁胺混悬液+夹尾刺激”复制气滞型STC大鼠模型,连续刺... 目的 基于SCF/c-kit信号通路探讨理气通便方对气滞型慢传输型便秘(STC)大鼠肠道动力的影响。方法 将36只Wistar雌性大鼠按随机数字表法分为对照组6只和造模组30只。造模组采用“洛哌丁胺混悬液+夹尾刺激”复制气滞型STC大鼠模型,连续刺激14 d。当大鼠表现出激惹、易怒、烦躁等情况,正常喂养饲料时出现大便干硬、排便数量减少等表现时,说明气滞型便秘模型造模成功。将造模成功的大鼠按照随机数字表法分为模型组、西药组、低剂量组、中剂量组和高剂量组各6只,低、中、高剂量组分别按5.15、10.3、20.6 g/(kg·d)给予理气通便方药液灌胃;西药组按0.18 mg/(kg·d)给予琥珀酸普芦卡必利片混悬液灌胃;对照组和模型组按10 mL/(kg·d)给予无菌水灌胃,每日1次,连续灌胃14 d。比较6组末次给药后6 h的粪便排出量、粪便含水量和小肠推进率;HE染色观察结肠组织病理变化;免疫组化检测结肠组织c-kit蛋白表达水平;Western blot检测结肠组织c-kit、SCF蛋白表达量。结果 HE染色显示:对照组大鼠结肠黏膜完整,杯状细胞和腺体排列整齐,未见炎症细胞聚集;模型组结肠黏膜出现肠腺排列欠整齐,黏膜下层间质血管扩张;各给药组结肠黏膜肠腺排列整齐,未观察到明显上皮损伤。与对照组比较,模型组粪便排出量、粪便含水量和小肠推进率均明显降低(P<0.05),结肠组织c-kit蛋白表达水平和c-kit、SCF蛋白表达量均明显降低(P<0.05)。与模型组比较,西药组、中剂量组、高剂量组粪便排出量、粪便含水率和小肠推进率均明显提高(P<0.05),低剂量组粪便排出量和粪便含水率均明显提高(P<0.05);给药组结肠组织c-kit蛋白表达水平均明显提高(P<0.05);低、中、高剂量组结肠组织c-kit蛋白表达量均明显提高(P<0.05),高剂量组SCF蛋白表达量明显提高(P<0.05)。结论 理气通便方可提高气滞型STC模型大鼠结肠组织中ICC的表达及调控SCF/c-kit信号通路,恢复对胃肠道节律的正常调控来改善便秘的症状。 展开更多
关键词 慢传输型便秘 气滞 理气通便方 ICC scf/c-kit信号通路
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二陈汤通过调控SCF/C-kit通路介导的痰湿型脑出血急性期胃肠功能障碍的研究
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作者 匡逸 鄢伟 +3 位作者 张晓菲 唐明 张增 王春燕 《环球中医药》 CAS 2024年第8期1499-1506,共8页
目的通过观察二陈汤对痰湿证脑出血急性期大鼠胃肠组织干细胞因子(stemcellfactor,SCF)/干细胞因子受体(C-kit)信号通路的动态变化的影响,探讨痰湿型体质与脑出血急性期胃肠功能障碍的关系及二陈汤的作用机制。方法将60只SD大鼠随机均... 目的通过观察二陈汤对痰湿证脑出血急性期大鼠胃肠组织干细胞因子(stemcellfactor,SCF)/干细胞因子受体(C-kit)信号通路的动态变化的影响,探讨痰湿型体质与脑出血急性期胃肠功能障碍的关系及二陈汤的作用机制。方法将60只SD大鼠随机均分为空白组、假手术组、模型组、莫沙必利组(0.27 mg/d)、二陈汤低剂量组和二陈汤高剂量组(217、434 mg/d),每组10只。除空白组、假手术组外其余各组均采用自体血注入法制备大鼠脑出血模型,各组按剂量给予灌胃,空白组、假手术组、模型组给予生理盐水灌胃,每天2次,于给药7天处死大鼠,采用苏木精—伊红(hematoxylin-eosin,HE)染色法观察大鼠胃肠黏膜细胞形态变化,采用实时荧光定量PCR(Real-time PCR,RT-PCR)法和蛋白印迹(Western blot,WB)法检测大鼠胃、小肠组织中的SCF、C-kit蛋白及mRNA的表达水平。结果光镜下见空白组、假手术组大鼠胃肠黏膜细胞结构完整、形态规则,偶见轻微充血现象;模型组见组织细胞充血、水肿、炎性细胞浸润明显、组织糜烂;莫沙必利组及二陈汤低、高剂量组胃肠黏膜各层次欠清晰、排列不规则,部分组织可见组织细胞充血、水肿、炎性细胞浸润,此3组中以二陈汤低剂量组胃肠黏膜表现最为严重。模型组大鼠胃肠组织中SCF、C-kit mRNA表达水平较空白组、假手术组显著降低(P<0.05),莫沙必利组、二陈汤低剂量组、二陈汤高剂量组的表达增加(P<0.05),莫沙必利组和二陈汤高剂量组大鼠胃组织中的SCF mRNA表达显著增加(P<0.05),二陈汤高剂量组C-kit mRNA表达显著增加(P<0.05);小肠组织中的SCF、C-kit mRNA含量随二陈汤浓度提高而增加(P<0.05)。与模型组相比,莫沙必利组、二陈汤低、高剂量组大鼠胃肠组织中SCF、C-kit蛋白表达水平较模型组显著升高(P<0.05),三组中以莫沙必利及高剂量二陈汤效果更佳(P<0.05)。结论脑出血急性期痰湿型胃肠黏膜损伤及功能障碍的作用机制与SCF/C-kit信号通路相关蛋白及mRNA的表达降低有关,二陈汤可通过此机制进行有效干预,上调相关蛋白及mRNA的表达,有利于脑出血急性期胃肠功能障碍恢复。 展开更多
关键词 脑出血急性期 胃肠功能功能障碍 scf/C-kit通路 二陈汤 痰湿证
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Strigolactones modulate cotton fiber elongation and secondary cell wall thickening 被引量:2
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作者 Yunze Wen Peng He +3 位作者 Xiaohan Bai Huizhi Zhang Yunfeng Zhang Jianing Yu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期1850-1863,共14页
Cotton is one of the most important economic crops in the world,and it is a major source of fiber in the textile industry.Strigolactones(SLs)are a class of carotenoid-derived plant hormones involved in many processes ... Cotton is one of the most important economic crops in the world,and it is a major source of fiber in the textile industry.Strigolactones(SLs)are a class of carotenoid-derived plant hormones involved in many processes of plant growth and development,although the functions of SL in fiber development remain largely unknown.Here,we found that the endogenous SLs were significantly higher in fibers at 20 days post-anthesis(DPA).Exogenous SLs significantly increased fiber length and cell wall thickness.Furthermore,we cloned three key SL biosynthetic genes,namely GhD27,GhMAX3,and GhMAX4,which were highly expressed in fibers,and subcellular localization analyses revealed that GhD27,GhMAX3,and GhMAX4 were localized in the chloroplast.The exogenous expression of GhD27,GhMAX3,and GhMAX4 complemented the physiological phenotypes of d27,max3,and max4 mutations in Arabidopsis,respectively.Knockdown of GhD27,GhMAX3,and GhMAX4 in cotton resulted in increased numbers of axillary buds and leaves,reduced fiber length,and significantly reduced fiber thickness.These findings revealed that SLs participate in plant growth,fiber elongation,and secondary cell wall formation in cotton.These results provide new and effective genetic resources for improving cotton fiber yield and plant architecture. 展开更多
关键词 STRIGOLACTONES fiber elongation secondary cell wall thickening COTTON
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Thermo-hydro-poro-mechanical responses of a reservoir-induced landslide tracked by high-resolution fiber optic sensing nerves 被引量:3
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作者 Xiao Ye Hong-Hu Zhu +4 位作者 Gang Cheng Hua-Fu Pei Bin Shi Luca Schenato Alessandro Pasuto 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第3期1018-1032,共15页
Thermo-poro-mechanical responses along sliding zone/surface have been extensively studied.However,it has not been recognized that the potential contribution of other crucial engineering geological interfaces beyond th... Thermo-poro-mechanical responses along sliding zone/surface have been extensively studied.However,it has not been recognized that the potential contribution of other crucial engineering geological interfaces beyond the slip surface to progressive failure.Here,we aim to investigate the subsurface multiphysics of reservoir landslides under two extreme hydrologic conditions(i.e.wet and dry),particularly within sliding masses.Based on ultra-weak fiber Bragg grating(UWFBG)technology,we employ specialpurpose fiber optic sensing cables that can be implanted into boreholes as“nerves of the Earth”to collect data on soil temperature,water content,pore water pressure,and strain.The Xinpu landslide in the middle reach of the Three Gorges Reservoir Area in China was selected as a case study to establish a paradigm for in situ thermo-hydro-poro-mechanical monitoring.These UWFBG-based sensing cables were vertically buried in a 31 m-deep borehole at the foot of the landslide,with a resolution of 1 m except for the pressure sensor.We reported field measurements covering the period 2021 and 2022 and produced the spatiotemporal profiles throughout the borehole.Results show that wet years are more likely to motivate landslide motions than dry years.The annual thermally active layer of the landslide has a critical depth of roughly 9 m and might move downward in warmer years.The dynamic groundwater table is located at depths of 9e15 m,where the peaked strain undergoes a periodical response of leap and withdrawal to annual hydrometeorological cycles.These interface behaviors may support the interpretation of the contribution of reservoir regulation to slope stability,allowing us to correlate them to local damage events and potential global destabilization.This paper also offers a natural framework for interpreting thermo-hydro-poro-mechanical signatures from creeping reservoir bank slopes,which may form the basis for a landslide monitoring and early warning system. 展开更多
关键词 Reservoir landslide Thermo-hydro-poro-mechanical response Ultra-weak fiber bragg grating(UWFBG) subsurface evolution Engineering geological interface Geotechnical monitoring
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Genome-wide identification of the pectate lyase(PEL)gene family members in Malvaceae,and their contribution to cotton fiber quality 被引量:1
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作者 Qian Deng Zeyu Dong +5 位作者 Zequan Chen Zhuolin Shi Ting Zhao Xueying Guan Yan Hu Lei Fang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第10期3264-3282,共19页
Pectin is a major constituent of the plant cell wall.Pectate lyase(PEL,EC 4.2.2.2)uses anti-β-elimination chemistry to cleave theα-1,4 glycosidic linkage in the homogalacturonan region of pectin.However,limited info... Pectin is a major constituent of the plant cell wall.Pectate lyase(PEL,EC 4.2.2.2)uses anti-β-elimination chemistry to cleave theα-1,4 glycosidic linkage in the homogalacturonan region of pectin.However,limited information is available on the comprehensive and evolutionary analysis of PELs in the Malvaceae.In this study,we identified 597PEL genes from 10 Malvaceae species.Phylogenetic and motif analyses revealed that these PELs are classified into six subfamilies:Clades I,II,III,IV,Va,and Vb.The two largest subfamilies,Clades I and II,contained 237 and222 PEL members,respectively.The members of Clades Va and Vb only contained four or five motifs,far fewer than the other subfamilies.Gene duplication analysis showed that segmental duplication played a crucial role in the expansion of the PEL gene family in Gossypium species.The PELs from Clades I,IV,Va,and Vb were expressed during the fiber elongation stage,but nearly all PEL genes from Clades II and III showed no expression in any of the investigated fiber developmental stages.We further performed single-gene haplotype association analysis in 2,001G.hirsutum accessions and 229 G.barbadense accessions.Interestingly,14 PELs were significantly associated with fiber length and strength traits in G.barbadense with superior fiber quality,while only eight GhPEL genes were found to be significantly associated with fiber quality traits in G.hirsutum.Our findings provide important information for further evolutionary and functional research on the PEL gene family members and their potential use for fiber quality improvement in cotton. 展开更多
关键词 MALVACEAE pectate lyase(PEL) Gossypium species fiber quality
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Coherent optical frequency transfer via 972-km fiber link 被引量:1
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作者 邓雪 张翔 +8 位作者 臧琦 焦东东 王丹 刘杰 高静 许冠军 董瑞芳 刘涛 张首刚 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第2期277-282,共6页
We demonstrate coherent optical frequency dissemination over a distance of 972 km by cascading two spans where the phase noise is passively compensated for.Instead of employing a phase discriminator and a phase lockin... We demonstrate coherent optical frequency dissemination over a distance of 972 km by cascading two spans where the phase noise is passively compensated for.Instead of employing a phase discriminator and a phase locking loop in the conventional active phase control scheme,the passive phase noise cancellation is realized by feeding double-trip beat-note frequency to the driver of the acoustic optical modulator at the local site.This passive scheme exhibits fine robustness and reliability,making it suitable for long-distance and noisy fiber links.An optical regeneration station is used in the link for signal amplification and cascaded transmission.The phase noise cancellation and transfer instability of the 972-km link is investigated,and transfer instability of 1.1×10^(-19)at 10^(4)s is achieved.This work provides a promising method for realizing optical frequency distribution over thousands of kilometers by using fiber links. 展开更多
关键词 optical frequency transfer fiber link phase noise cancellation
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Genetic dissection and origin of pleiotropic loci underlying multilevel fiber quality traits in upland cotton(Gossypium hirsutum L.) 被引量:1
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作者 Hongge Li Shurong Tang +7 位作者 Zhen Peng Guoyong Fu Yinhua Jia Shoujun Wei Baojun Chen Muhammad Shahid Iqbal Shoupu He Xiongming Du 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第10期3250-3263,共14页
Cotton fiber quality is a persistent concern that determines planting benefits and the quality of finished textile products.However,the limitations of measurement instruments have hindered the accurate evaluation of s... Cotton fiber quality is a persistent concern that determines planting benefits and the quality of finished textile products.However,the limitations of measurement instruments have hindered the accurate evaluation of some important fiber characteristics such as fiber maturity,fineness,and neps,which in turn has impeded the genetic improvement and industrial utilization of cotton fiber.Here,12 single fiber quality traits were measured using Advanced Fiber Information System(AFIS)equipment among 383 accessions of upland cotton(Gossypium hirsutum L.).In addition,eight conventional fiber quality traits were assessed by the High Volume Instrument(HVI)System.Genome-wide association study(GWAS),linkage disequilibrium(LD)block genotyping and functional identification were conducted sequentially to uncover the associated elite loci and candidate genes of fiber quality traits.As a result,the previously reported pleiotropic locus FL_D11 regulating fiber length-related traits was identified in this study.More importantly,three novel pleiotropic loci(FM_A03,FF_A05,and FN_A07)regulating fiber maturity,fineness and neps,respectively,were detected based on AFIS traits.Numerous highly promising candidate genes were screened out by integrating RNA-seq and qRT-PCR analyses,including the reported GhKRP6 for fiber length,the newly identified GhMAP8 for maturity and GhDFR for fineness.The origin and evolutionary analysis of pleiotropic loci indicated that the selection pressure on FL_D11,FM_A03 and FF_A05 increased as the breeding period approached the present and the origins of FM_A03 and FF_A05 were traced back to cotton landraces.These findings reveal the genetic basis underlying fiber quality and provide insight into the genetic improvement and textile utilization of fiber in G.hirsutum. 展开更多
关键词 upland cotton single fiber quality pleiotropic loci candidate genes locus origin
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Evaluation of choroidal thickness and retinal nerve fiber layer thickness in Chinese pregnant women and healthy non-pregnant women 被引量:1
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作者 Hanfei Wu Haishuang Lin +5 位作者 Mengting Ruan Huanjie Fang Nannan Dong Tiewei Wang Feifei Yu Jiawei Zhao 《Advances in Ophthalmology Practice and Research》 2024年第1期8-13,共6页
Purpose:To evaluate choroidal thickness and retinal nerve fiber layer(RNFL)thickness in different trimesters using enhanced depth imaging optical coherence tomography(EDI-OCT).Methods:A prospective comparative study i... Purpose:To evaluate choroidal thickness and retinal nerve fiber layer(RNFL)thickness in different trimesters using enhanced depth imaging optical coherence tomography(EDI-OCT).Methods:A prospective comparative study included 45 healthy pregnant women in the first trimester,45 women in the second,45 women in the third and 45 healthy non-pregnant women as the control group.Macular choroidal thickness was measured at three locations:The subfoveal,1 mm temporal,and 1 mm nasal from the fovea with EDI-OCT.Peripapillary choroidal thickness(PPCT)and RNFL thickness parameters were automatically calculated by the Spectralis OCT.Results:The subfoveal,temporal and nasal macular choroidal thickness were all significantly thicker in the second trimester,compared with those parameters in the first,the third trimesters and the control group(all P<0.05).The PPCT was significantly increased in the second trimeter compared with the control group at global,temporal,temporal inferior,nasal and nasal inferior positions(all P<0.05).The RNFL thickness was also significantly increased in pregnant women at nasal superior and nasal inferior quadrants(all P<0.05).Conclusions:The choroidal thickness in pregnant women was found to be thicker than the control group,regardless of macular or optic disc location.Findings of RNFL thickening might indicate subclinical involvement of the central nervous system. 展开更多
关键词 CHOROID Retinal nerve fiber layer Optical coherence tomography PREGNANCY
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SCF泛素连接酶的体外泛素化体系构建与检测
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作者 胡健健 梅文聪 +1 位作者 张文慧 刘主 《华中农业大学学报》 CAS CSCD 北大核心 2024年第2期273-279,共7页
为研究蛋白质的体外泛素化过程,利用大肠杆菌表达系统异源表达和纯化APPBP1/UBA3(E1)、UBC12(E2)、Cullin1-Rbx1(E3)和Nedd8(neural precursor cell expressed developmentally down-regulated protein 8)蛋白,制备FITC-Cysteine绿色荧... 为研究蛋白质的体外泛素化过程,利用大肠杆菌表达系统异源表达和纯化APPBP1/UBA3(E1)、UBC12(E2)、Cullin1-Rbx1(E3)和Nedd8(neural precursor cell expressed developmentally down-regulated protein 8)蛋白,制备FITC-Cysteine绿色荧光素标记的泛素Ub(Ubiquitin),构建了SCF泛素连接酶的体外泛素化体系,同时实现快速检测SCF泛素连接酶中Cullin1蛋白的自泛素化修饰。结果表明,建立的体外SCF E3自泛素化活性反应体系具有较高的可操作性和便利性。 展开更多
关键词 scf泛素连接酶 泛素化修饰 Cullin1蛋白 荧光标记
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基于深度学习的兰姆波SCF-TFM超分辨率成像
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作者 孙刘家 韩庆邦 +1 位作者 靳琪琳 葛考 《仪器仪表学报》 EI CAS CSCD 北大核心 2024年第6期101-110,共10页
腐蚀和裂纹是结构板常见的缺陷形式,兰姆波在非贯穿型损伤处发生模式转换是制约兰姆波成像质量的主要因素。此外,声波衍射遵循瑞利准则,超声成像存在分辨率极限。本文设计了一个全卷积神经网络对接收信号进行分割与重构,实现目标模态的... 腐蚀和裂纹是结构板常见的缺陷形式,兰姆波在非贯穿型损伤处发生模式转换是制约兰姆波成像质量的主要因素。此外,声波衍射遵循瑞利准则,超声成像存在分辨率极限。本文设计了一个全卷积神经网络对接收信号进行分割与重构,实现目标模态的自动拾取,抹除杂波和模式转换的干扰。提出符号相干因子全聚焦成像法(SCF-TFM),在全矩阵聚焦成像过程中施加符号相干因子,抑制非目标区域散射波对成像结果的干扰,同时考虑散射信号的幅值及相位信息,可以一定程度上突破瑞利准则的限制,实现超分辨率成像。实验结果表明:对于单个盲孔缺陷,该方法成像结果的横向分辨率比全聚焦提高62.41%,信噪比提升58.23%;而对于多个非对称盲孔缺陷,当缺陷间距大于瑞利准则分辨率极限时,该方法的信噪比提高了92.89%;缺陷间距小于瑞利准则分辨率极限时,该方法可以实现超分辨率成像。 展开更多
关键词 兰姆波 非对称盲孔缺陷 全卷积神经网络 scf-TFM 超分辨率成像
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Analysis of piezoelectric semiconductor fibers under gradient temperature changes 被引量:1
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作者 Shuangpeng LI Ruoran CHENG +1 位作者 Nannan MA Chunli ZHANG 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2024年第2期311-320,共10页
Piezoelectric semiconductors(PSs)possess both semiconducting properties and piezoelectric coupling effects,making them optimal building blocks for semiconductor devices.PS fiber-like structures have wide applications ... Piezoelectric semiconductors(PSs)possess both semiconducting properties and piezoelectric coupling effects,making them optimal building blocks for semiconductor devices.PS fiber-like structures have wide applications in multi-functional semiconductor devices.In this paper,a one-dimensional(1D)theoretical model is established to describe the piezotronic responses of a PS fiber under gradient temperature changes.The theoretical model aims to explain the mechanism behind the resistance change caused by such gradient temperature changes.Numerical results demonstrate that a gradient temperature change significantly affects the physical fields within the PS fiber,and can induce changes in its surface resistance.It provides important theoretical guidance on the development of piezotronic devices that are sensitive to temperature effects. 展开更多
关键词 piezoelectric semiconductor(PS)fiber one-dimensional(1D)model piezotronic effect gradient temperature change
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超临界二氧化碳流体中SCFX-AYRL染料的溶解性研究
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作者 赵向阳 闫凯 龙家杰 《纺织学报》 EI CAS CSCD 北大核心 2024年第6期98-104,共7页
染料在超临界二氧化碳流体(SCF-CO_(2))中的良好溶解性是超临界无水染色技术应用的前提和基础。采用研制的超临界流体溶解度测试装置,研究了活性分散红SCFX-AYRL专用染料在SCF-CO_(2)中的溶解性,并分别从流体处理时间、温度、系统压力... 染料在超临界二氧化碳流体(SCF-CO_(2))中的良好溶解性是超临界无水染色技术应用的前提和基础。采用研制的超临界流体溶解度测试装置,研究了活性分散红SCFX-AYRL专用染料在SCF-CO_(2)中的溶解性,并分别从流体处理时间、温度、系统压力和助溶剂应用方面对该专用染料溶解性的影响进行了探讨。研究表明当系统压力为20 MPa、温度为80~130℃时,SCF-CO_(2)中该染料在30~60 min可达到溶解平衡,在一定范围内升高流体温度和系统压力可提高专用染料的溶解性;加入助溶剂可显著改善染料的溶解行为,提高其溶解度及溶解速率,降低温度、压力等系统因素的影响;Chrastil经验模型可实现对SCF-CO_(2)和SCF-CO_(2)/助溶剂体系中该专用染料溶解行为的良好关联和预报。 展开更多
关键词 超临界二氧化碳流体 活性分散染料 溶解性 助溶剂 溶解度关联
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基于SCF/C-kit通路探讨三焦针法对腹泻型肠易激综合征大鼠的影响
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作者 尚雪梅 朱洲 +5 位作者 潘莉 唐徐韵 宋光仙 石丽杉 程敏 杨孝芳 《环球中医药》 CAS 2024年第4期578-584,共7页
目的 观察三焦针法对腹泻型肠易激综合征(irritable bowel syndrome with diarrhea, IBS-D)大鼠胃肠动力异常及结肠组织干细胞因子(stem cell factor, SCF)、酪氨酸激酶受体(tyrosine kinase receptor, C-kit) mRNA和蛋白表达的影响,探... 目的 观察三焦针法对腹泻型肠易激综合征(irritable bowel syndrome with diarrhea, IBS-D)大鼠胃肠动力异常及结肠组织干细胞因子(stem cell factor, SCF)、酪氨酸激酶受体(tyrosine kinase receptor, C-kit) mRNA和蛋白表达的影响,探讨三焦针法治疗IBS-D的作用机制。方法 wistar雄性大鼠随机分为空白组、模型组、西药组和三焦组,每组6只。除空白组外,其余各组采用“冰醋酸灌肠+束缚应激”建立IBS-D大鼠模型。西药组予匹维溴胺灌胃(15 mg/kg),三焦组针刺膻中、中脘、气海、血海(双侧)及足三里(双侧),诸穴行捻转补法30秒,1次/d,均治疗14天。观察大鼠一般状态;测定体质量、旷场实验(大鼠横向运动和纵向运动)、稀便率、腹壁撤退反射(abdominal retraction reflex, AWR)3分阈值时注水量;RT-PCR法、Western blot法检测结肠组织SCF、C-kit mRNA及蛋白表达。结果 与空白组相比,IBS-D模型大鼠一般状态较差,体质量显著降低(P<0.01),旷场实验检测横向运动距离与纵向运动次数显著降低(P<0.01),稀便率显著增高(P<0.01),AWR3分时注水量显著降低(P<0.01),结肠组织中SCF、C-kit mRNA及蛋白表达水平均显著升高(P<0.01)。与模型组比较,西药组、三焦组大鼠体质量显著升高(P<0.01,P<0.05),横向运动距离与纵向运动次数均显著升高(P<0.05,P<0.01),稀便率显著下调(P<0.01),AWR3分时注水量显著提升(P<0.01),三焦组大鼠结肠组织中SCF、C-kit mRNA及蛋白水平表达均显著降低(P<0.01),而西药组SCF、C-kit mRNA及蛋白表达无统计学意义(P>0.05)。结论 三焦针法可改善IBS-D大鼠症状,可能是与调节结肠组织中SCF、C-kit mRNA及蛋白表达水平有关。 展开更多
关键词 腹泻型肠易激综合征 三焦针法 scf/C-kit通路 胃肠动力
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Wavelength-interval switchable Brillouin–Raman random fiber laser through Brillouin pump manipulation
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作者 李阳 徐恩明 +2 位作者 陈睿佳 Yu-Gang Shee 张祖兴 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第7期374-379,共6页
A wavelength-interval switchable Brillouin–Raman random fiber laser(BRRFL) based on Brillouin pump(BP) manipulation is proposed in this paper. The proposed wavelength-interval switchable BRRFL has a full-open cavity ... A wavelength-interval switchable Brillouin–Raman random fiber laser(BRRFL) based on Brillouin pump(BP) manipulation is proposed in this paper. The proposed wavelength-interval switchable BRRFL has a full-open cavity configuration, featuring multiwavelength output with wavelength interval of double Brillouin frequency shifts. Through simultaneously injecting the BP light and its first-order stimulated Brillouin-scattered light into the cavity, the laser output exhibits a wavelength interval of single Brillouin frequency shift. The wavelength-interval switching effect can be manipulated by controlling the power of the first-order stimulated Brillouin scattering light. The experimental results show the multiwavelength output can be switched between double Brillouin frequency shift multiwavelength emission with a broad bandwidth of approximately 60 nm and single Brillouin frequency shift multiwavelength emission of 44 nm. The flexible optically controlled random fiber laser with switchable wavelength interval makes it useful for a wide range of applications and holds significant potential in the field of wavelength-division multiplexing optical communication. 展开更多
关键词 wavelength-interval switchable fiber laser Brillouin fiber laser stimulated Brillouin-scattered random fiber laser
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Real-Time 4-Mode MDM Transmission Using Commercial 400G OTN Transceivers and All-Fiber Mode Multiplexers 被引量:1
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作者 REN Fang LI Yidan +2 位作者 YE Bing LIU Jianguo CHEN Weizhang 《ZTE Communications》 2024年第1期106-110,共5页
Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low inse... Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low insertion loss and modal crosstalk are the key components.In this paper,a low-modal-crosstalk 4-mode MMUX/MDEMUX for the weakly-coupled triple-ring-core few-mode fiber(TRC-FMF)is designed and fabricated with side-polishing processing.The measurement results show that a pair of MMUX/MDEMUX and 25 km weakly-coupled TRC-FMF MDM link achieve low modal crosstalk of lower than−17.5 dB and insertion loss of lower than 11.56 dB for all the four modes.Based on the TRC-FMF and all-fiber MMUX/MDEMUX,an experiment for 25 km real-time 4-mode 3-λwavelength division multiplexing(WDM)-MDM transmission is conducted using commercial 400G optical transport network(OTN)transceivers.The experimental results prove weakly-coupled MDM techniques facilitate a smooth upgrade of the optical transmission system. 展开更多
关键词 optical fiber couplers mode(de)multiplexers mode division multiplexing transmission
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Controllable large-scale processing of temperature regulating sheath-core fibers with high-enthalpy for thermal management
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作者 Ziye Chen Zexu Hu +4 位作者 Shining Chen Senlong Yu Liping Zhu Hengxue Xiang Meifang Zhu 《Nano Materials Science》 EI CAS CSCD 2024年第3期337-344,共8页
Temperature regulating fibers(TRF_(s)) with high enthalpy and high form stability are the key factors for thermal management. However, the enthalpies of most TRFsare not high, and the preparation methods are still at ... Temperature regulating fibers(TRF_(s)) with high enthalpy and high form stability are the key factors for thermal management. However, the enthalpies of most TRFsare not high, and the preparation methods are still at the laboratory scale. It remains a great challenge to use industrial spinning equipment to achieve continuous processing of TRF_(s) with excellent thermal and mechanical properties. Here, polyamide 6(PA6) based TRF_(s) with a sheath-core structure were prepared by bicomponent melt-spinning. The sheath-core TRF(TRF_(sc)) are composed of PA6 as sheath and functional PA6 as core, which are filled with the shape stable phase change materials(ssPCM),dendritic silica@polyethylene glycol(SiO_(2)@PEG). With the aid of the sheath structure, the filling content of SiO_(2)@PEG can reach 30 %, so that the enthalpy of the TRF_(s) can be as high as 21.3 J/g. The ultra-high enthalpy guarantees the temperature regulation ability during the alternating process of cooling and heating. In hot environment, the temperature regulation time is 6.59 min, and the temperature difference is 12.93℃. In addition, the mechanical strength of the prepared TRF_(sc) reaches 2.26 cN/dtex, which can fully meet its application in the field of thermal management textiles and devices to manage the temperature regulation of the human body or precision equipment, etc. 展开更多
关键词 Thermal management Hybrid fibers Polyamides Bicomponent melt spinning Temperature regulating fibers
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Robust Transfer of Optical Frequency over 500km Fiber Link with Instability of 10^(−21)
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作者 Qian Zhou Xiang Zhang +6 位作者 Qi Zang Mengfan Wu Dan Wang Jie Liu Ruifang Dong Tao Liu Shougang Zhang 《Chinese Physics Letters》 SCIE EI CAS CSCD 2024年第8期22-27,共6页
Our primary objective is to mitigate the adverse effects of temperature fluctuations on the optical frequency transmission system by reducing the length of the interferometer.Following optimization,the phase-temperatu... Our primary objective is to mitigate the adverse effects of temperature fluctuations on the optical frequency transmission system by reducing the length of the interferometer.Following optimization,the phase-temperature coefficient of the optical system is reduced to approximately 1.35 fs/K.By applying a sophisticated temperature control to the remained“out-of-loop”optics fiber,the noise floor of the system has been effectively lowered to 10−21 level.Based on this performance-enhanced transfer system,we demonstrate coherent transmission of optical frequency through 500-km spooled fiber link.After being actively compensated,the transfer instability of 4.5×10^(−16) at the averaging time of 1 s and 5.6×10^(−21) at 10000 s is demonstrated.The frequency uncertainty of received light at remote site relative to that of the origin light at local site is achieved to be 1.15×10^(−19).This enhanced system configuration is particularly well suited for future long-distance frequency transmission and comparison of the most advanced optical clock signals. 展开更多
关键词 fiber INTERFEROMETER system
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