The ultrafast monitoring of deoxyribonucleic acid(DNA)dynamic structural changes is an emerging and rapidly growing research topic in biotechnology.The existing optical spectroscopy used to identify different dynamica...The ultrafast monitoring of deoxyribonucleic acid(DNA)dynamic structural changes is an emerging and rapidly growing research topic in biotechnology.The existing optical spectroscopy used to identify different dynamical DNA structures lacks quick response while requiring large consumption of samples and bulky instrumental facilities.It is highly demanded to develop an ultrafast technique that monitors DNA structural changes with the external stimulus or cancer-related disease scenarios.Here,we demonstrate a novel photonic integrated graphene-optofluidic device to monitor DNA structural changes with the ultrafast response time.Our approach is featured with an effective and straightforward design of decoding the electronic structure change of graphene induced by its interactions with DNAs in different conformations using ultrafast nanosecond pulse laser and achieving refractive index sensitivity of~3×10^(−5) RIU.This innovative technique for the first time allows us to perform ultrafast monitoring of the conformational changes of special DNA molecules structures,including G-quadruplex formation by K+ions and i-motif formation by the low pH stimulus.The graphene-optofluidic device as presented here provides a new class of label-free,ultrafast,ultrasensitive,compact,and cost-effective optical biosensors for medical and healthcare applications.展开更多
Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sens...Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sensitivity,high signal-to-noise ratio,and narrow linewidth,but also hold the unique features of optical fiber,including ease of integration,high repeatability,and low cost.With the development of new fiber structures and fabrication technologies,FOFLs become an important branch of optical fiber sensors,especially for application in biochemical detection.In this paper,the recent progress on FOFL is reviewed.We focuse mainly on the optical fiber resonators,gain medium,and the emerging sen sing applicatio ns.The prospects for FOFL are also discussed.We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.展开更多
基金from the National Natural Science Foundation of China(21874096,21575095,51602305,61604102 and 61875139)the 111 Project,and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)+2 种基金the China Postdoctoral Science Foundation(2018M633118)Shenzhen Nanshan District Pilotage Team Program(LHTD20170006)Australian Research Council(ARC,FT150100450,IH150100006 and CE170100039).Q.Bao acknowledges support from the Australian Research Council(ARC)Centre of Excellence in Future Low-Energy Electronics Technologies(FLEET).
文摘The ultrafast monitoring of deoxyribonucleic acid(DNA)dynamic structural changes is an emerging and rapidly growing research topic in biotechnology.The existing optical spectroscopy used to identify different dynamical DNA structures lacks quick response while requiring large consumption of samples and bulky instrumental facilities.It is highly demanded to develop an ultrafast technique that monitors DNA structural changes with the external stimulus or cancer-related disease scenarios.Here,we demonstrate a novel photonic integrated graphene-optofluidic device to monitor DNA structural changes with the ultrafast response time.Our approach is featured with an effective and straightforward design of decoding the electronic structure change of graphene induced by its interactions with DNAs in different conformations using ultrafast nanosecond pulse laser and achieving refractive index sensitivity of~3×10^(−5) RIU.This innovative technique for the first time allows us to perform ultrafast monitoring of the conformational changes of special DNA molecules structures,including G-quadruplex formation by K+ions and i-motif formation by the low pH stimulus.The graphene-optofluidic device as presented here provides a new class of label-free,ultrafast,ultrasensitive,compact,and cost-effective optical biosensors for medical and healthcare applications.
基金the financial support from the National Natural Science Foundation of China(Grant No.61875034)the 111 Project(Grant No.B14039)Seeding Project of Scientific and Technical Innovation of Sichuan Province(Grant No.2020107).
文摘Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sensitivity,high signal-to-noise ratio,and narrow linewidth,but also hold the unique features of optical fiber,including ease of integration,high repeatability,and low cost.With the development of new fiber structures and fabrication technologies,FOFLs become an important branch of optical fiber sensors,especially for application in biochemical detection.In this paper,the recent progress on FOFL is reviewed.We focuse mainly on the optical fiber resonators,gain medium,and the emerging sen sing applicatio ns.The prospects for FOFL are also discussed.We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.
