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血清4型禽腺病毒Fiber-1蛋白截短表达及多克隆抗体制备
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作者 罗昕雨 赵磊 +5 位作者 陈玉晴 缪欣怡 石家鑫 顾有方 李文超 刘欣超 《安徽科技学院学报》 2024年第2期25-30,共6页
目的:制备血清4型禽腺病毒(FAdV-4)Fiber-1基因截短蛋白多克隆抗体,为FAdV-4病的诊断、检测及致病机制研究奠定基础。方法:对FAdV-4的CH/AHMC/2015分离株Fiber-1基因序列(MG148335.1:30459-31754)进行信号肽、疏水性和抗原决定簇分析,... 目的:制备血清4型禽腺病毒(FAdV-4)Fiber-1基因截短蛋白多克隆抗体,为FAdV-4病的诊断、检测及致病机制研究奠定基础。方法:对FAdV-4的CH/AHMC/2015分离株Fiber-1基因序列(MG148335.1:30459-31754)进行信号肽、疏水性和抗原决定簇分析,截取具有较高免疫原性的片段,设计合成特异性引物,以CH/AHMC/2015分离株基因组DNA为模板进行PCR扩增,获得截短Fiber-1(sFiber-1)基因片段,将其连接至原核表达载体pET-32a,验证后转化至大肠杆菌BL21感受态中,诱导表达sFiber-1蛋白。纯化后的蛋白与佐剂乳化后免疫SD大鼠,制备多克隆抗体,并测定多克隆抗体效价。用Western-blot检测抗体免疫原性。结果:成功构建了sFiber-1的原核表达载体,获得FAdV-4的sFiber-1重组蛋白,经SDS-PAGE鉴定,重组sFiber-1蛋白大小约为52 kDa,主要以包涵体形式表达;间接ELISA法测得sFiber-1多克隆抗体效价为1∶2^(13);Western blot结果显示制备的多克隆抗体能特异性识别出重组sFiber-1蛋白。结论:本研究成功表达了FAdV-4的重组sFiber-1蛋白,制备了具有较高免疫活性的sFiber-1多克隆抗体,可为FAdV-4的检测及诊断奠定基础。 展开更多
关键词 血清4型禽腺病毒 fiber-1基因 重组蛋白 多克隆抗体
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Characterization and Modeling of Mechanical Properties of Additively Manufactured Coconut Fiber-Reinforced Polypropylene Composites
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作者 George Mosi Bernard W. Ikua +1 位作者 Samuel K. Kabini James W. Mwangi 《Advances in Materials Physics and Chemistry》 CAS 2024年第6期95-112,共18页
In the face of the increased global campaign to minimize the emission of greenhouse gases and the need for sustainability in manufacturing, there is a great deal of research focusing on environmentally benign and rene... In the face of the increased global campaign to minimize the emission of greenhouse gases and the need for sustainability in manufacturing, there is a great deal of research focusing on environmentally benign and renewable materials as a substitute for synthetic and petroleum-based products. Natural fiber-reinforced polymeric composites have recently been proposed as a viable alternative to synthetic materials. The current work investigates the suitability of coconut fiber-reinforced polypropylene as a structural material. The coconut fiber-reinforced polypropylene composites were developed. Samples of coconut fiber/polypropylene (PP) composites were prepared using Fused Filament Fabrication (FFF). Tests were then conducted on the mechanical properties of the composites for different proportions of coconut fibers. The results obtained indicate that the composites loaded with 2 wt% exhibited the highest tensile and flexural strength, while the ones loaded with 3 wt% had the highest compression strength. The ultimate tensile and flexural strength at 2 wt% were determined to be 34.13 MPa and 70.47 MPa respectively. The compression strength at 3 wt% was found to be 37.88 MPa. Compared to pure polypropylene, the addition of coconut fibers increased the tensile, flexural, and compression strength of the composite. In the study, an artificial neural network model was proposed to predict the mechanical properties of polymeric composites based on the proportion of fibers. The model was found to predict data with high accuracy. 展开更多
关键词 Additive Manufacturing Artificial Neural Network Mechanical Properties Natural Fibers POLYPROPYLENE
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鸭腺病毒3型Fiber-2蛋白单克隆抗体的制备及初步应用 被引量:2
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作者 魏常青 史馨瑾 +4 位作者 吕璐 刘英楠 谢振华 陈宗艳 陈鸿军 《中国家禽》 北大核心 2023年第2期46-51,共6页
为进一步研究鸭腺病毒3型(DAdV-3)Fiber-2蛋白,研究通过PCR扩增fiber-2基因,构建p ET32a-DAdV3-fiber2原核质粒,表达并纯化蛋白,以纯化的蛋白为免疫原,免疫6周龄雌性BALB/c小鼠制备抗Fiber-2单克隆抗体杂交瘤细胞,采用间接免疫荧光试验... 为进一步研究鸭腺病毒3型(DAdV-3)Fiber-2蛋白,研究通过PCR扩增fiber-2基因,构建p ET32a-DAdV3-fiber2原核质粒,表达并纯化蛋白,以纯化的蛋白为免疫原,免疫6周龄雌性BALB/c小鼠制备抗Fiber-2单克隆抗体杂交瘤细胞,采用间接免疫荧光试验和免疫印迹试验进行鉴定,并用于DAdV-3 Fiber-2杆状病毒表达产物的鉴定。结果显示:原核表达获得1.77 mg/mL Fiber-2蛋白,筛选获得2株阳性单克隆细胞株2E10E4和4F7F10,两株单克隆抗体均为IgG 1型,轻链类型为κ;DAdV-3感染的LMH细胞中存在特异性荧光,免疫印迹试验确认在约59 ku处出现特异性条带;采用制备的单克隆抗体确认了表达Fiber-2蛋白的重组杆状病毒。研究获得了针对DAdV-3 Fiber-2蛋白的单克隆抗体,为深入探究Fiber-2在DAdV-3感染机制中的作用以及为建立DAdV-3抗原免疫测定技术奠定了基础。 展开更多
关键词 DAdV-3 fiber-2 单克隆抗体
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Ⅰ群禽腺病毒(4型)Fiber-2蛋白亚单位疫苗与全病毒灭活疫苗的免疫效果比较 被引量:1
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作者 杜东颖 霍环艳 +5 位作者 田辉 王璐璐 王孟月 洪素梅 高晓静 田克恭 《中国动物传染病学报》 CAS 北大核心 2023年第3期67-73,共7页
为了比较不同抗原含量的Ⅰ群禽腺病毒(4型)Fiber-2蛋白亚单位疫苗与全病毒灭活疫苗的免疫效力差异,本研究制备不同抗原含量的亚单位疫苗和全病毒灭活疫苗,采用超低免疫剂量20μL/只免疫SPF鸡,免疫21 d后攻毒,通过死亡率、临床症状、大... 为了比较不同抗原含量的Ⅰ群禽腺病毒(4型)Fiber-2蛋白亚单位疫苗与全病毒灭活疫苗的免疫效力差异,本研究制备不同抗原含量的亚单位疫苗和全病毒灭活疫苗,采用超低免疫剂量20μL/只免疫SPF鸡,免疫21 d后攻毒,通过死亡率、临床症状、大体剖检病变、免疫组化和泄殖腔拭子排毒等进行免疫效力比较。试验结果表明,亚单位疫苗抗原含量AGP效价不低于1∶8,全病毒灭活疫苗抗原含量为10^(7.0)TCID_(50)/0.1 mL时,可抵抗Ⅰ群禽腺病毒(4型)强毒株的攻击,提供100%的免疫攻毒保护,攻毒后3、5、7 d泄殖腔拭子均未见排毒,且免疫组化均为阴性。亚单位疫苗抗原含量AGP效价为1∶4,全病毒灭活疫苗抗原含量为106.5 TCID_(50)/0.1 mL时,在超低免疫剂量情况下,仍可提供至少80%的免疫攻毒保护,且攻毒后3、5、7 d泄殖腔拭子排毒率仅为2/10~3/10,免疫组化阳性率仅为1/10。但全病毒灭活疫苗抗原含量为106.0 TCID_(50)/0.1 mL时,免疫攻毒保护效果不理想。综合以上结果表明,Fiber-2蛋白具有良好的免疫原性,抗原含量AGP效价不低于1∶4,与全病毒灭活疫苗抗原含量为106.5 TCID_(50)/0.1 mL免疫效力相当,可用于疫苗研制开发。 展开更多
关键词 禽腺病毒 fiber-2蛋白 亚单位疫苗 全病毒灭活疫苗 免疫攻毒保护
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禽腺病毒4型fiber-2与8b型fiber截短融合蛋白的原核表达及其免疫原性分析 被引量:2
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作者 迟丽丽 王君娜 +5 位作者 张宇名 刘健 陈志远 李树凡 尹燕博 徐守振 《畜牧兽医学报》 CAS CSCD 北大核心 2023年第12期5162-5170,共9页
利用原核表达系统表达Ⅰ群禽腺病毒血清4型(FAdV-4)fiber2和8b型(FAdV-8b)fiber全长蛋白、截短蛋白及二价融合蛋白,并评价其免疫原性。基于FAdV-4 fiber2和FAdV-8b fiber抗原性好的截短片段(FP和BP),利用SOE-PCR构建二价融合片段(BP-FP)... 利用原核表达系统表达Ⅰ群禽腺病毒血清4型(FAdV-4)fiber2和8b型(FAdV-8b)fiber全长蛋白、截短蛋白及二价融合蛋白,并评价其免疫原性。基于FAdV-4 fiber2和FAdV-8b fiber抗原性好的截短片段(FP和BP),利用SOE-PCR构建二价融合片段(BP-FP),分别对截短蛋白、融合蛋白及全长(FL和BL)在大肠杆菌中进行表达,采用SDS-PAGE和Western blot对重组蛋白进行鉴定。纯化后的重组蛋白免疫SPF鸡并攻毒,计算各组存活率、间接ELISA检测免疫鸡血清抗体水平、qPCR检测泄殖腔拭子排毒量和肝脏中细胞因子IL-4、IFN-γ和TNF-αmRNA表达水平以及进行组织病理观察。结果显示:构建的重组质粒均在大肠杆菌中成功可溶表达;BP-FP4组存活率为58.3%,其他组存活率均为100%;全长组(FL组和BL组)抗体水平均高于各自片段组(FP组和BP组),但差异不显著(P>0.05);攻毒后3和7 d C8b组排毒量均显著高于免疫组(P<0.05),攻毒后7 d BP-FP8b组排毒量显著高于BP和BL组(P<0.05),BL组和BP组差异不显著(P>0.05);免疫组IL-4、IFN-γ和TNF-αmRNA表达均显著高于阴性对照组(P<0.05),FL组IFN-γ和TNF-αmRNA表达均显著低于BP-FP4组(P<0.05),FL组和FP组差异不显著(P>0.05),BL组IL-4和IFN-γmRNA表达均显著低于BP-FP8b组(P<0.05)。组织病理观察发现,攻毒组肝脏病变最严重,FP组与BP组次之,FL组与BL组无明显病变。本研究表明FAdV-4 fiber2和FAdV-8b fiber的全长蛋白和截短蛋白免疫保护效果优于融合蛋白,截短蛋白可替代全长蛋白用于制备禽腺病毒亚单位疫苗。 展开更多
关键词 禽腺病毒 纤突蛋白 原核表达 亚单位疫苗
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基于血清4型禽腺病毒Fiber-2间接ELISA方法的建立 被引量:8
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作者 梅楠 孙海伟 +3 位作者 李允章 汪凯 王桂军 陈鸿军 《中国预防兽医学报》 CAS CSCD 北大核心 2019年第8期807-811,共5页
为建立一种快速监测鸡群中血清4型禽腺病毒(FAdV-4)的方法,本研究通过PCR扩增FAd V-4 AQ强毒株的fiber-2基因截短体(112 bp^1 440 bp),克隆至p ET-32a(+)载体中进行原核表达,以重组表达的Fiber-2蛋白作为包被抗原,经优化反应条件,建立... 为建立一种快速监测鸡群中血清4型禽腺病毒(FAdV-4)的方法,本研究通过PCR扩增FAd V-4 AQ强毒株的fiber-2基因截短体(112 bp^1 440 bp),克隆至p ET-32a(+)载体中进行原核表达,以重组表达的Fiber-2蛋白作为包被抗原,经优化反应条件,建立了快速检测FAd V-4的间接ELISA方法,该方法特异性试验结果显示,除FAd V-4外,与其它病原无交叉反应;敏感性试验结果显示,该方法检测血清最低稀释度为1∶12 800,具有较高的敏感性;重复性试验结果显示,该方法批内、批间变异系数均小于10%。该方法对FAd V-4临床感染的阳性血清检出率为100%,与琼脂扩散试验结果完全吻合。本研究建立的以Fiber-2重组蛋白为包被抗原的间接ELISA方法能够用于FAd V-4灭活疫苗免疫后的血清学调查。 展开更多
关键词 血清4型禽腺病毒 FAdV-4 fiber-2 原核表达 间接ELISA
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血清4型禽腺病毒Fiber-2蛋白截短表达及单克隆抗体制备 被引量:6
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作者 韦悠 谢芝勋 +6 位作者 邓显文 李小凤 谢志勤 范晴 张艳芳 黄娇玲 王盛 《南方农业学报》 CAS CSCD 北大核心 2022年第8期2341-2349,共9页
【目的】获得截短表达的血清4型禽腺病毒(FAdV-4)Fiber-2重组蛋白及其单克隆抗体,为建立快速灵敏的病毒检测方法提供基础材料。【方法】对Fiber-2基因序列进行密码子优化,截取Fiber-2蛋白抗原性集中片段,PCR扩增其基因序列,连接至pET-32... 【目的】获得截短表达的血清4型禽腺病毒(FAdV-4)Fiber-2重组蛋白及其单克隆抗体,为建立快速灵敏的病毒检测方法提供基础材料。【方法】对Fiber-2基因序列进行密码子优化,截取Fiber-2蛋白抗原性集中片段,PCR扩增其基因序列,连接至pET-32a(+)构建原核表达载体。将表达的重组蛋白进行纯化,并通过Western blotting验证其在大肠杆菌中的表达情况。将重组蛋白免疫BALB/c小鼠后取脾细胞与SP2/0细胞融合,利用间接酶联免疫吸附试验(ELISA)法筛选阳性细胞株,再利用秋水仙素裂解法、间接ELISA法和间接免疫荧光法(IFA)对其进行鉴定。【结果】成功构建Fiber-2基因截短片段的原核表达系统,获得大小约为67 kD的Fiber-2截短蛋白,以包涵体的形式出现,经纯化后可获得单一的特异性条带,经Western blotting鉴定证明其可与FAdV-4阳性血清结合从而产生一条特异性条带。经过4次亚克隆后筛选到5株能稳定分泌抗体的杂交瘤细胞株(2C2、4F6、5A5、6G10和10B5),其抗体分泌稳定性、特异性好,诱生的细胞上清液抗体效价为1:1600~1:6400,腹水抗体效价为1:320000~1:1280000,秋水仙素裂解检测染色体数目为94~110条。2C2、4F6和10B5杂交瘤细胞分泌的抗体重链为IgG2b亚型,轻链为Kappa链;5A5和6G10杂交瘤细胞分泌的抗体重链为IgG1亚型,轻链为Kappa链。5株杂交瘤细胞株与感染FAdV-4的鸡肝癌上皮细胞系(LMH)进行IFA检测,结果均为阳性。【结论】制备的Fiber-2截短重组蛋白及单克隆抗体具有良好的反应原性和特异性,可用于FAdV-4病毒检测试剂盒研发。 展开更多
关键词 血清4型禽腺病毒(FAdV-4) fiber-2 基因克隆 重组蛋白 单克隆抗体 Western blotting
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Fiber-FISH在植物基因组研究中的应用
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作者 刘国庆 朱立煌 《生物工程进展》 CSCD 2001年第3期71-73,共3页
Fiber FISH是近几年发展起来的一项高分辨率和高灵敏度的荧光原位杂交技术 ,已被广泛应用于人类及动植物基因组的研究。本文对Fiber FISH在植物基因组研究中的特点及其在染色体物理图谱的构建 ,染色体结构与分子构成分析和在比较基因组... Fiber FISH是近几年发展起来的一项高分辨率和高灵敏度的荧光原位杂交技术 ,已被广泛应用于人类及动植物基因组的研究。本文对Fiber FISH在植物基因组研究中的特点及其在染色体物理图谱的构建 ,染色体结构与分子构成分析和在比较基因组中的应用等进行了探讨。 展开更多
关键词 fiber-FISH 植物 基因组 应用
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1群4型禽腺病毒的分离鉴定及Fiber-2蛋白的免疫效果分析 被引量:6
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作者 程增青 范根成 +4 位作者 窦小龙 刘红祥 申茂欣 徐全刚 杨汉春 《畜牧兽医学报》 CAS CSCD 北大核心 2020年第10期2463-2471,共9页
旨在鉴定河南某蛋鸡场以心包积液、肝肿大出血为特征的病原,并对病原的免疫原性蛋白进行表达和效果分析。本研究采用病毒分离、血清学试验、PCR检测及测序分析、动物回归试验、大肠杆菌表达、蛋白纯化、攻毒保护等方法进行研究。结果显... 旨在鉴定河南某蛋鸡场以心包积液、肝肿大出血为特征的病原,并对病原的免疫原性蛋白进行表达和效果分析。本研究采用病毒分离、血清学试验、PCR检测及测序分析、动物回归试验、大肠杆菌表达、蛋白纯化、攻毒保护等方法进行研究。结果显示,所采集样品经卵黄囊途径接种7日龄SPF鸡胚,盲传2代后获得病毒;PCR可扩增出约900 bp的Hexon基因片段,经测序,其Hexon基因与血清C4型毒株的相似性为100%;血清中和试验结果表明分离的禽腺病毒为1群血清4型禽腺病毒,命名为HN-ZK株。该毒株可在鸡胚原代肝细胞上产生CPE,病毒含量可达10^(7.5)TCID50·0.1 mL^-1。动物回归试验表明,该分离毒株可使35日龄SPF鸡100%(10/10)表现出发病症状。以分离株的DNA为模板,利用大肠杆菌原核表达系统,获得相对分子质量约为33 ku的Fiber-2蛋白,离心浓缩、纯化后蛋白含量为300 mg·mL^-1。将表达的Fiber-2蛋白用不同的剂量免疫SPF鸡,结果表明20μg·只^-1的剂量能使鸡完全抵抗强毒株的攻击,说明Fiber-2蛋白具有较好的免疫原性。本研究可为禽心包积水-肝炎综合征的诊断和基因工程亚单位疫苗的研制提供数据参考。 展开更多
关键词 I群禽腺病毒 鉴定 fiber-2蛋白 亚单位疫苗
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血清1型Ⅰ群禽腺病毒Fiber-2蛋白的表达及其中和作用研究 被引量:1
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作者 高永娟 栾庆东 +6 位作者 曲雪婷 原昆鹏 王潘龙 赵梓靓 卢艳敬 尹燕博 王建琳 《中国家禽》 北大核心 2022年第8期35-41,共7页
为研究血清1型Ⅰ群禽腺病毒(FAdV-1)的Fiber-2蛋白对FAdV-1在细胞水平上的中和作用,研究根据FAdV-1亚型Fiber-2基因序列设计引物,进行Fiber-2全基因序列PCR扩增并构建重组质粒,转入BL21(DE3)感受态细胞,经IPTG诱导表达,表达产物纯化后免... 为研究血清1型Ⅰ群禽腺病毒(FAdV-1)的Fiber-2蛋白对FAdV-1在细胞水平上的中和作用,研究根据FAdV-1亚型Fiber-2基因序列设计引物,进行Fiber-2全基因序列PCR扩增并构建重组质粒,转入BL21(DE3)感受态细胞,经IPTG诱导表达,表达产物纯化后免疫SPF鸡制备抗血清,通过鸡LMH细胞研究Fiber-2蛋白抗血清对FAdV-1感染的中和作用。结果显示,FAdV-1 Fiber-2基因扩增产物大小为1 233 bp,构建了pCold-FAdV-1-Fiber-2重组质粒,表达的FAdV-1 Fiber-2蛋白分子量大小为105 ku;FAdV-1 Fiber-2蛋白的抗血清孵育LMH细胞后,与阳性对照组相同第4天细胞出现病变,而阴性对照组细胞均无病变。结果表明,FAdV-1 Fiber-2蛋白抗血清在体外试验中不能中和本血清型病毒的感染,FAdV-1 Fiber-2蛋白作为亚单位疫苗的研究存在风险。 展开更多
关键词 血清1型Ⅰ群禽腺病毒 fiber-2蛋白 LMH细胞 中和作用
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禽腺病毒4型Fiber-2蛋白原核表达及间接ELISA的构建 被引量:1
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作者 潘静 孟凯 秦晓冰 《山东农业科学》 北大核心 2021年第11期100-105,共6页
禽腺病毒(FAdV)是世界范围内流行的禽类常见传染病病原,2015年暴发的由新型血清4型禽腺病毒(FAdV-4)引起的急性传染病,给养殖户造成巨大的经济损失。为做到早期诊断和检测,并防控、净化该病毒病,本试验利用Fiber-2蛋白建立了检测FAdV-4... 禽腺病毒(FAdV)是世界范围内流行的禽类常见传染病病原,2015年暴发的由新型血清4型禽腺病毒(FAdV-4)引起的急性传染病,给养殖户造成巨大的经济损失。为做到早期诊断和检测,并防控、净化该病毒病,本试验利用Fiber-2蛋白建立了检测FAdV-4的ELISA方法。根据禽腺病毒4型SDJN0105株Fiber-2基因的核苷酸序列,经序列优化后合成1 440 bp基因序列,连入pET-30a(+)载体构建pET-30a(+)-Fiber-2重组质粒,在BL21(DE3)感受态细胞中诱导表达Fiber-2蛋白,利用亲和层析柱纯化重组Fiber-2蛋白,经SDS-PAGE和Western-Blot鉴定后,以Fiber-2蛋白作为包被抗原,通过反应条件优化建立检测禽腺病毒4型抗体的间接ELISA方法。结果表明,获得了以上清形式表达的重组Fiber-2蛋白,Western-Blot鉴定表明重组Fiber-2蛋白具有良好的免疫学活性;重组Fiber-2蛋白的最佳包被浓度为4.0μg/mL,待检血清最佳稀释度为1∶400;该方法特异性好,仅与禽腺病毒4型阳性抗体发生反应,敏感度可达1∶6400;批内和批间重复性变异系数均小于5%;构建的间接ELISA试剂盒在4℃环境下放置12个月仍然有效。本研究建立的禽腺病毒4型抗体间接ELISA检测方法特异性强、敏感性高、重复性好、保质期长,可用于禽腺病毒4型的监测和流行病学调查。 展开更多
关键词 禽腺病毒4型 fiber-2蛋白 原核表达 间接ELISA
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血清4型禽腺病毒fiber-2基因在昆虫细胞中的表达及其免疫原性分析 被引量:6
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作者 梅梅 陆吉虎 +3 位作者 张雪花 唐应华 卢宇 侯继波 《中国预防兽医学报》 CAS CSCD 北大核心 2019年第12期1251-1255,共5页
为研究I群血清4型禽腺病毒(FAdV-4)fiber-2蛋白的免疫原性,本研究利用杆状病毒表达系统构建了含fiber-2基因的重组杆状病毒穿梭载体,将其转染Sf9细胞后获得重组杆状病毒rBA-fiber-2,并采用悬浮放大工艺表达重组fiber-2蛋白。通过间接免... 为研究I群血清4型禽腺病毒(FAdV-4)fiber-2蛋白的免疫原性,本研究利用杆状病毒表达系统构建了含fiber-2基因的重组杆状病毒穿梭载体,将其转染Sf9细胞后获得重组杆状病毒rBA-fiber-2,并采用悬浮放大工艺表达重组fiber-2蛋白。通过间接免疫荧光试验(IFA)和western blot鉴定重组蛋白fiber-2的表达,并采用免疫琼扩试验(AGP)检测fiber-2蛋白效价。以fiber-2蛋白作为免疫原制备油乳剂疫苗免疫SPF鸡进行免疫效力试验,分别设fiber-2疫苗组、fiber-2疫苗配伍免疫增强剂CVCVA5组及生理盐水对照组。免疫后14 d、21 d和28 d采集SPF鸡外周血分离血清,利用AGP法检测其血清抗体水平。IFA结果显示fiber-2蛋白在昆虫细胞中获得表达;western blot结果显示该蛋白能够与FAdV-4阳性血清发生特异性免疫反应,蛋白分子量约62 ku;AGP结果显示fiber-2蛋白AGP效价达1:16。免疫后14 d两组实验组鸡均可检测到特异性琼扩抗体,免疫后28 d fiber-2疫苗组SPF鸡抗体AGP效价平均值可达1:24,配伍CVCVA5疫苗组SPF鸡抗体AGP效价平均值可达1:104。以上结果表明fiber-2蛋白具有一定的免疫原性,免疫后可诱导机体产生特异性抗体,本研究为进一步研制FAdV-4亚单位疫苗提供实验依据。 展开更多
关键词 血清4型禽腺病毒 纤突蛋白 表达 免疫原性
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Ⅰ群禽腺病毒(4型)Fiber-2蛋白亚单位疫苗免疫效果研究 被引量:3
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作者 杜东颖 盛东北 +7 位作者 霍环艳 段佳蕾 王孟月 谭镜明 陈宇明 胡鹏辉 刘武杰 田克恭 《中国动物传染病学报》 CAS 北大核心 2022年第6期77-82,共6页
为评价Ⅰ群禽腺病毒(4型)Fiber-2重组蛋白亚单位疫苗的免疫保护效果,本研究利用FAV-HN株Fiber-2基因构建pET-30a-Fiber-2重组质粒,转化至BL21感受态诱导表达Fiber-2重组蛋白;Fiber-2蛋白纯化后制备不同抗原含量的亚单位疫苗,免疫SPF鸡... 为评价Ⅰ群禽腺病毒(4型)Fiber-2重组蛋白亚单位疫苗的免疫保护效果,本研究利用FAV-HN株Fiber-2基因构建pET-30a-Fiber-2重组质粒,转化至BL21感受态诱导表达Fiber-2重组蛋白;Fiber-2蛋白纯化后制备不同抗原含量的亚单位疫苗,免疫SPF鸡进行免疫效力评价。结果表明,Fiber-2蛋白部分为可溶性表达,纯化后纯度约90%,AGP效价达1∶64。亚单位疫苗抗原含量AGP效价不低于1∶2时,SPF鸡免疫后第21 d AGP抗体10/10阳性,可抵抗Ⅰ群禽腺病毒(4型)强毒株的攻击,提供100%的免疫攻毒保护。 展开更多
关键词 禽腺病毒 fiber-2蛋白 亚单位疫苗 免疫攻毒保护
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Fiber-reinforced Mechanism and Mechanical Performance of Composite Fibers Reinforced Concrete 被引量:4
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作者 申俊敏 ZHANG Yancong 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2020年第1期121-130,共10页
To understand the enhancing effect and fiber-reinforced mechanism of composite fibers reinforced cement concrete, the influences of composite fibers on micro-cracks and the distribution of composite fibers were evalua... To understand the enhancing effect and fiber-reinforced mechanism of composite fibers reinforced cement concrete, the influences of composite fibers on micro-cracks and the distribution of composite fibers were evaluated by optical electron micrometer(OEM) and scanning electron microscope(SEM). Three kinds of fiber, such as polyacrylonitrile-based carbon fiber, basalt fiber, and glass fiber, were used in the composite fibers reinforced cement concrete. The composite fibers could form a stable structure in concrete after the liquid-phase coupling treatment, gas-liquid double-effect treatment, and inert atmosphere drying. The mechanical properties of composite fibers reinforced concrete(CFRC) were studied by universal test machine(UTM). Moreover, the effect of composite fibers on concrete was analyzed based on the toughness index and residual strength index. The results demonstrated that the composite fibers could improve the mechanical properties of concrete, while the excessive amount of composite fibers had an adverse effect on the mechanical properties of concrete. The composite fibers could significantly improve the toughness index of CFRC, and the increment rate is more than 30%. The composite fibers could form a mesh structure, which could promote the stability of concrete and guarantee the excellent mechanical properties. 展开更多
关键词 CEMENT CONCRETE composite fibers mechanical performance fiber-reinforced mechanism
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In-situ growth of vertically aligned nickel cobalt sulfide nanowires on carbon nanotube fibers for high capacitance all-solid-state asymmetric fiber-supercapacitors 被引量:5
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作者 Na Liu Zhenghui Pan +6 位作者 Xiaoyu Ding Jie Yang Guoguang Xu Linge Li Qi Wang Meinan Liu Yuegang Zhang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2020年第2期209-215,共7页
Fiber-supercapacitors(FSCs)are promising power sources for miniature portable and wearable electronic devices.However,the development and practical application of these FSCs have been severely hindered by their low vo... Fiber-supercapacitors(FSCs)are promising power sources for miniature portable and wearable electronic devices.However,the development and practical application of these FSCs have been severely hindered by their low volumetric capacitance and narrow operating voltage.In this work,vertically aligned nickel cobalt sulfide(Ni Co2S4)nanowires grown on carbon nanotube(CNT)fibers were achieved through an in-situ two-step hydrothermal reaction method.The as-prepared Ni Co2S4@CNT fiber electrode exhibits a high volumetric capacitance of 2332 F cm-3,benefiting from its superior electric conductivity,large surface area,and rich Faradic redox reaction sites.Furthermore,a Ni Co2S4@CNT//VN@CNT(vanadium nitride nanosheets grown on CNT fibers)asymmetric fiber-supercapacitor(AFSC)was successfully fabricated.The device exhibits an operating voltage up to 1.6 V and a high volumetric energy density of 30.64m Wh cm-3.The device also possesses outstanding flexibility as evidenced by no obvious performance degradation under various bending angles and maintaining high capacitance after 5000 bending cycles.This work promotes the practical application of flexible wearable energy-storage devices. 展开更多
关键词 fiber-supercapacitors Volumetric energy density NICKEL COBALT SULFIDE NANOWIRES Carbon nanotube FIBER Wearable energy storage devices
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Effects of dietary fiber content and different fiber-rich ingredients on endogenous loss of fat and fatty acids in growing pigs 被引量:3
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作者 Yifan Chen Zhenyu Wang +5 位作者 Jian Ding Dongxu Ming Wenhui Wang Zhaoning Jiang Ling Liu Fenglai Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2019年第3期745-758,共14页
Background: Determination of the endogenous loss of fat(ELF) is used to adjust for the estimation of true total tract digestibility(TTTD) of fat in diets and ingredients.Any factor which affected ELF may further affec... Background: Determination of the endogenous loss of fat(ELF) is used to adjust for the estimation of true total tract digestibility(TTTD) of fat in diets and ingredients.Any factor which affected ELF may further affect the digestibility of fat,including sources and concentrations of fat and fiber in the diet.There are some reports of determining the ELF using regression methods based on different levels of fat intake,while reports on effects of dietary fiber content and different fiber-rich ingredients in pig diets on ELF are very limited.Therefore,the objective of this study was to determine the effects of dietary fiber content and different fiber-rich ingredients on endogenous losses of fat and fatty acids at the end of ileum and throughout the entire intestinal tract in growing pigs.Methods: In Exp.1,the effect of fiber content on endogenous loss of fat was determined using six growing pigs(Duroc × Landrace × Yorkshire;27.6 ± 2.4 kg),fitted with a T-cannula at the end of ileum.The experimental design was a 6 × 6 complete Latin square design with six periods of feeding and six diets.The six experimental fat-free diets were formulated to include graded levels of neutral detergent fiber(NDF)(0,40,80,120,160 and 200 g/kg) and soybean hull(SH) was the only fiber source,providing 0,75,150,225,300 and 375 g/kg,respectively.Chromic oxide was included at4 g/kg in all diets as an indigestible marker.In Exp.2,six crossbred growing barrows(27.6 ± 1.6 kg) were used and the experimental design was the same as for Exp.1.The six fat-free diets were formulated to include six common fiber-rich ingredients and the concentration of NDF was 100 g/kg.The six fiber-rich ingredients were defatted rice bran(DRB),sugar beet pulp(SBP),rice hull(RH),corn germ meal(CGM),SH and wheat bran(WB) and they were fed at represented250,270,145,250,170 and 280 g/kg in the diet,respectively.Results: In Exp.1,the endogenous loss of fatty acids profile did not change as dietary NDF increased in growing pigs.The endogenous losses of fat,C16:0,C18:0,C18:1,C18:2,total unsaturated fatty acids(UFA) and total saturated fatty acids(SFA) in growing pigs at the end of ileum and throughout the entire intestinal tract increased linearly as NDF content of diets increased.The endogenous losses of fat,as well as C16:0 and C18:0 throughout the entire intestinal tract also increased quadratically as NDF content of diets increased.The ELF increased from 0.71 to 3.14 g/kg of dry matter intake(DMI) and 0.56 to 8.21 g/kg DMI at the end of ileum and throughout the entire intestinal tract in growing pigs,respectively.The ELF occurred in the hindgut except for the growing pigs fed 0 and 4% NDF in their diets.The endogenous losses of C16:0 and UFA occurred primarily in the upper regions of the gut and the greatest endogenous losses of C18:0 occurred in the hindgut.The endogenous losses of fat,individual SFA and total SFA throughout the entire intestinal tract were much greater than that at the end of ileum.However,the endogenous losses of individual UFA and total UFA were less throughout the intestinal tract than at the end of ileum.In Exp.2,the endogenous losses of fat at the end of ileum were greater in growing pigs fed CGM or WB diets.The endogenous loss of fatty acids profile changed to a slight degree at the end of ileum that the endogenous loss of UFA(particularly C18:1 and C18:2) in growing pigs fed CGM or WB diets were greater(P < 0.01) than that for the other four diets.The greatest(P < 0.01) endogenous loss of SFA(particularly C18:0) was in growing pigs fed the RH diet.The endogenous losses of fat,C16:0,C18:0 and SFA over the entire intestinal tract were much greater in growing pigs fed CGM or WB diets,whereas the lowest values were in growing pigs fed DRB diet.The ELF at the end of ileum in growing pigs fed CGM or WB diets were 3.50 or 4.17 g/kg DMI,respectively,and the ELF over the entire intestinal tract was 7.23 or 7.38 g/kg DMI.The contribution in percentage of ELF in the upper gut was greater than that in the hindgut of growing pigs fed DRB and RH diets,while the ELF in the upper gut and hindgut were equal in growing pigs fed SBP,CGM and WB diets.On the whole,the endogenous losses of C18:1 and C18:2 throughout the entire intestinal tract in growing pigs fed the six fiber-rich ingredients diets were less than losses at the end of ileum,whereas the endogenous loss of fat,C16:0,C18:0 and SFA were greater throughout the intestinal tract than at the end of ileum.Conclusion: The profile of loss in endogenous fatty acids did not change as dietary NDF increased in growing pigs and the endogenous losses of fatty acids(C16:0,C18:0,C18:1 and C18:2) fat,UFA and SFA increased linearly as NDF content increased in the diets of pigs.The endogenous losses of fat or fatty acids at the end of ileum were greater in growing pigs fed RH,CGM or WB diets.The endogenous losses of fat,fatty acids(C16:0 and C18:0) and SFA were greater over the entire intestinal tract in pigs fed CGM or WB diet,while these values were the lowest in growing pigs fed the DRB diet.The contribution in percentage losses of fat in the upper gut were greater than in the hindgut of growing pigs fed DRB and RH diets,while the contribution of losses of fat in the upper gut and hindgut were equal in growing pigs fed SBP,CGM and WB diets.In addition,the endogenous loss of individual or total UFA was less over the entire intestinal tract of growing pigs fed fiber diets than that at the end of ileum,and the greatest endogenous losses of fat,individual or total SFA were over the entire intestinal tract.Therefore,differences in fiber content and the nature of fiber-rich ingredients in diets of pigs have different effects to the endogenous losses of fat or fatty acids.Considering the requirement of fat or fatty acids of pigs,careful attention must be paid that the endogenous losses of fat and fatty acids when fiber ingredients are used in diets of pigs. 展开更多
关键词 ENDOGENOUS LOSS FAT Fatty acids FIBER content fiber-rich INGREDIENTS Growing pigs
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禽腺病毒血清4型Fiber-2蛋白在大肠杆菌中可溶性表达及其免疫原性分析 被引量:2
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作者 耿笑林 王孟月 +6 位作者 张翼 刘鹏 李可乐 杜东颖 逄文强 黄玉欣 田克恭 《畜牧与兽医》 北大核心 2021年第8期94-99,共6页
旨在解决大肠杆菌表达系统对禽腺病毒血清4型(FAdV-4) Fiber-2蛋白进行表达时存在的多包涵体问题。通过添加不同的化学伴侣分子来提升Fiber-2的可溶性表达量,并进一步通过动物试验评价蛋白的免疫原性。结果显示,添加化学伴侣分子β-环... 旨在解决大肠杆菌表达系统对禽腺病毒血清4型(FAdV-4) Fiber-2蛋白进行表达时存在的多包涵体问题。通过添加不同的化学伴侣分子来提升Fiber-2的可溶性表达量,并进一步通过动物试验评价蛋白的免疫原性。结果显示,添加化学伴侣分子β-环糊精显著提高了Fiber-2的可溶性表达量,且重组蛋白与FAdV-4阳性血清具有良好的反应性,琼脂扩散(AGP)效价达1∶128;以表达的可溶性Fiber-2蛋白作为抗原,制备油乳剂疫苗并免疫SPF鸡,通过AGP检测免疫后血清中针对FAdV的特异性抗体水平,结果Fiber-2蛋白疫苗及FAdV-4全病毒灭活疫苗于免疫后14 d,均可诱生特异性的AGP抗体。以上结果表明β-环糊精显著提高了Fiber-2的可溶性表达量,且免疫原性良好。本研究为FAdV-4亚单位疫苗的研制及产业化奠定了基础。 展开更多
关键词 禽腺病毒血清4型 fiber-2蛋白 化学伴侣分子 可溶性表达 免疫原性
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INTERFACIAL DEBONDING OF COATED-FIBER-REINFORCED COMPOSITES UNDER TENSION-TENSION CYCLIC LOADING 被引量:7
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作者 石志飞 周利民 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2000年第4期347-356,共10页
A new degradation function of the friction coefficient is used.Based on the double shear-lag model and Paris formula,the interracial damage of coated- fiber-reinforced composites under tension-tension cyclic loading i... A new degradation function of the friction coefficient is used.Based on the double shear-lag model and Paris formula,the interracial damage of coated- fiber-reinforced composites under tension-tension cyclic loading is studied.The effects of strength and thickness of the coating materials on the debond stress,debond rate as well as debond length are simulated. 展开更多
关键词 interfacial debonding cyclic loading COATING fiber-reinforced composite
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The Heat-swimming Force on the Fiber-like Particle in Noncon-formity Temperature Fluid 被引量:1
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作者 朱泽飞 林建忠 叶秉良 《Journal of Donghua University(English Edition)》 EI CAS 2001年第1期64-67,共4页
Fiber-like particle suspensions are common in both nature and industry, yet research on them is still in its infancy. This paper focuses on the theoretical analysis of the heat-swimming force operating on the fiber-li... Fiber-like particle suspensions are common in both nature and industry, yet research on them is still in its infancy. This paper focuses on the theoretical analysis of the heat-swimming force operating on the fiber-like particle in nonconformity temperature fluid, and the approximate calculating formula has been proposed. It is indicated that the heat- swimming force on the fiber-like particle is in direct proportion to thetemperature gradient of fluid, but it has opposite direction and that it is restrained by the particle volume, the fluid viscosity and density rather than by the particle density. 展开更多
关键词 multi-phase fluid fiber- like particle heat-swimming force .
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An Offloading Scheme Leveraging on Neighboring Node Resources for Edge Computing over Fiber-Wireless (FiWi) Access Networks 被引量:3
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作者 Wei Chang Yihong Hu +2 位作者 Guochu Shou Yaqiong Liu Zhigang Guo 《China Communications》 SCIE CSCD 2019年第11期107-119,共13页
The computation resources at a single node in Edge Computing(EC)are commonly limited,which cannot execute large scale computation tasks.To face the challenge,an Offloading scheme leveraging on NEighboring node Resourc... The computation resources at a single node in Edge Computing(EC)are commonly limited,which cannot execute large scale computation tasks.To face the challenge,an Offloading scheme leveraging on NEighboring node Resources(ONER)for EC over Fiber-Wireless(FiWi)access networks is proposed in this paper.In the ONER scheme,the FiWi network connects edge computing nodes with fiber and converges wireless and fiber connections seamlessly,so that it can support the offloading transmission with low delay and wide bandwidth.Based on the ONER scheme supported by FiWi networks,computation tasks can be offloaded to edge computing nodes in a wider range of area without increasing wireless hops(e.g.,just one wireless hop),which achieves low delay.Additionally,an efficient Computation Resource Scheduling(CRS)algorithm based on the ONER scheme is also proposed to make offloading decision.The results show that more offloading requests can be satisfied and the average completion time of computation tasks decreases significantly with the ONER scheme and the CRS algorithm.Therefore,the ONER scheme and the CRS algorithm can schedule computation resources at neighboring edge computing nodes for offloading to meet the challenge of large scale computation tasks. 展开更多
关键词 EDGE COMPUTING OFFLOADING fiber-wireless access networks delay
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