Damage in central nervous system plays an important role in biological life and causes severe paralysis of limbs and some organs. There are solutions to problems that can be a great revolution in the transplanted spin...Damage in central nervous system plays an important role in biological life and causes severe paralysis of limbs and some organs. There are solutions to problems that can be a great revolution in the transplanted spinal cord and nerve injuries. Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. The applications of SCs in regenerative medicine are limited because of slow growth rate and difficulties in harvesting. Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. We decided to use the three-dimensional culture of Schwann cells differentiated from endometrial stem cell in fibrin gel. In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair.展开更多
Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth...Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue,its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors.We herein sought to develop a bioactive bioink composed of i-PRF,alginate and gelatin capable of promoting the regeneration of the oral soft tissue.This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs.Importantly,we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF.The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts.When deployed in vivo in nude mice,this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity.Together,these data thus confirmthe successful production of an i-PRF-containing bioink,which is suitable for the individualized promotion of the regeneration of oral soft tissue.展开更多
【目的】观察加味温胆汤联合耳穴压豆治疗痰湿壅盛型高血压患者的临床疗效。【方法】将116例痰湿壅盛型高血压患者随机分为观察组和对照组,每组各58例,2组患者均给予常规检查与健康宣教,对照组给予硝苯地平控释片口服治疗,观察组在对照...【目的】观察加味温胆汤联合耳穴压豆治疗痰湿壅盛型高血压患者的临床疗效。【方法】将116例痰湿壅盛型高血压患者随机分为观察组和对照组,每组各58例,2组患者均给予常规检查与健康宣教,对照组给予硝苯地平控释片口服治疗,观察组在对照组治疗的基础上给予口服加味温胆汤联合耳穴压豆治疗。2组均连续治疗6周。治疗6周后,评价2组临床疗效,观察2组患者治疗前后血清人软骨糖蛋白-39(YKL-40)与人纤维胶凝蛋白3(ficolin-3)表达的变化情况,以及中医证候积分的变化情况。比较2组患者治疗前后动态血压及血压变异性的变化情况,包括24 h平均收缩压(24 h SBP)、24 h平均舒张压(24 h DBP)、24 h平均舒张压变异性(24 h DBPV)和24 h平均收缩压变异性(24 h SBPV)。并评价2组的安全性及并发症的发生情况。【结果】(1)治疗后,2组患者的血清YKL-40、ficolin-3水平均明显改善(P<0.05),且观察组在改善血清YKL-40、ficolin-3水平方面均明显优于对照组,差异均有统计学意义(P<0.05)。(2)治疗后,2组患者的中医证候积分均明显改善(P<0.05),且观察组在改善中医证候积分方面明显优于对照组,差异有统计学意义(P<0.05)。(3)治疗后,2组患者的24 h SBP、24 h DBP、24 h DBPV与24 h SBPV均明显改善(P<0.05),且观察组在改善24 h SBP、24 h DBP、24 h DBPV与24 h SBPV方面均明显优于对照组,差异均有统计学意义(P<0.05)。(4)观察组总有效率为94.83%(55/58),对照组为82.76%(48/58)。观察组疗效优于对照组,差异有统计学意义(P<0.05)。(5)观察组并发症总发生率为8.62%(5/58),对照组为6.90%(4/58)。观察组并发症总发生率稍高于对照组,但差异无统计学意义(P>0.05)。【结论】加味温胆汤联合耳穴压豆治疗痰湿壅盛型高血压,能够明显地改善患者血压变异性,降低血清YKL-40、ficolin-3水平,改善患者的临床症状,临床疗效显著。展开更多
文摘Damage in central nervous system plays an important role in biological life and causes severe paralysis of limbs and some organs. There are solutions to problems that can be a great revolution in the transplanted spinal cord and nerve injuries. Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. The applications of SCs in regenerative medicine are limited because of slow growth rate and difficulties in harvesting. Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. We decided to use the three-dimensional culture of Schwann cells differentiated from endometrial stem cell in fibrin gel. In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair.
基金supported by the National Key Research and Development Program of China(2017YFA0701302,PKUSS20200113).
文摘Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue,its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors.We herein sought to develop a bioactive bioink composed of i-PRF,alginate and gelatin capable of promoting the regeneration of the oral soft tissue.This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs.Importantly,we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF.The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts.When deployed in vivo in nude mice,this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity.Together,these data thus confirmthe successful production of an i-PRF-containing bioink,which is suitable for the individualized promotion of the regeneration of oral soft tissue.
文摘【目的】观察加味温胆汤联合耳穴压豆治疗痰湿壅盛型高血压患者的临床疗效。【方法】将116例痰湿壅盛型高血压患者随机分为观察组和对照组,每组各58例,2组患者均给予常规检查与健康宣教,对照组给予硝苯地平控释片口服治疗,观察组在对照组治疗的基础上给予口服加味温胆汤联合耳穴压豆治疗。2组均连续治疗6周。治疗6周后,评价2组临床疗效,观察2组患者治疗前后血清人软骨糖蛋白-39(YKL-40)与人纤维胶凝蛋白3(ficolin-3)表达的变化情况,以及中医证候积分的变化情况。比较2组患者治疗前后动态血压及血压变异性的变化情况,包括24 h平均收缩压(24 h SBP)、24 h平均舒张压(24 h DBP)、24 h平均舒张压变异性(24 h DBPV)和24 h平均收缩压变异性(24 h SBPV)。并评价2组的安全性及并发症的发生情况。【结果】(1)治疗后,2组患者的血清YKL-40、ficolin-3水平均明显改善(P<0.05),且观察组在改善血清YKL-40、ficolin-3水平方面均明显优于对照组,差异均有统计学意义(P<0.05)。(2)治疗后,2组患者的中医证候积分均明显改善(P<0.05),且观察组在改善中医证候积分方面明显优于对照组,差异有统计学意义(P<0.05)。(3)治疗后,2组患者的24 h SBP、24 h DBP、24 h DBPV与24 h SBPV均明显改善(P<0.05),且观察组在改善24 h SBP、24 h DBP、24 h DBPV与24 h SBPV方面均明显优于对照组,差异均有统计学意义(P<0.05)。(4)观察组总有效率为94.83%(55/58),对照组为82.76%(48/58)。观察组疗效优于对照组,差异有统计学意义(P<0.05)。(5)观察组并发症总发生率为8.62%(5/58),对照组为6.90%(4/58)。观察组并发症总发生率稍高于对照组,但差异无统计学意义(P>0.05)。【结论】加味温胆汤联合耳穴压豆治疗痰湿壅盛型高血压,能够明显地改善患者血压变异性,降低血清YKL-40、ficolin-3水平,改善患者的临床症状,临床疗效显著。
