The keratoprosthesis(KPro;artificial cornea)is a special refractive device to replace human cornea by using heterogeneous forming materials for the implantation into the damaged eyes in order to obtain a certain visio...The keratoprosthesis(KPro;artificial cornea)is a special refractive device to replace human cornea by using heterogeneous forming materials for the implantation into the damaged eyes in order to obtain a certain vision.The main problems of artificial cornea are the biocompatibility and stability of the tissue particularly in penetrating keratoplasty.The current studies of tissue-engineered scaffold materials through comprising composites of natural and synthetic biopolymers together have developed a new way to artificial cornea.Although a wide agreement that the long-term stability of these devices would be greatly improved by the presence of cornea cells,modification of keratoprosthesis to support cornea cells remains elusive.Most of the studies on corneal substrate materials and surface modification of composites have tried to improve the growth and biocompatibility of cornea cells which can not only reduce the stimulus of heterogeneous materials,but also more importantly continuous and stable cornea cells can prevent the destruction of collagenase.The necrosis of stroma and spontaneous extrusion of the device,allow for maintenance of a precorneal tear layer,and play the role of ensuring a good optical surface and resisting bacterial infection.As a result,improvement in corneal cells has been the main aim of several recent investigations;some effort has focused on biomaterial for its well biological properties such as promoting the growth of cornea cells.The purpose of this review is to summary the growth status of the corneal cells after the implantation of several artificial corneas.展开更多
Tissue engineering technology has advanced rapidly in recent years, offering opportunities to construct biologicallyactive tissues or organ substitutes to repair or even enhance the functions of diseased tissues and o...Tissue engineering technology has advanced rapidly in recent years, offering opportunities to construct biologicallyactive tissues or organ substitutes to repair or even enhance the functions of diseased tissues and organs.Tissue-engineered scaffolds rebuild the extracellular microenvironment by mimicking the extracellular matrix.Fibrin-based scaffolds possess numerous advantages, including hemostasis, high biocompatibility, and gooddegradability. Fibrin scaffolds provide an initial matrix that facilitates cell migration, differentiation, proliferation,and adhesion, and also play a critical role in cell-matrix interactions. Fibrin scaffolds are now widelyrecognized as a key component in tissue engineering, where they can facilitate tissue and organ defect repair.This review introduces the properties of fibrin, including its composition, structure, and biology. In addition, themodification and cross-linking modes of fibrin are discussed, along with various forms commonly used in tissueengineering. We also describe the biofunctionalization of fibrin. This review provides a detailed overview of theuse and applications of fibrin in skin, bone, and nervous tissues, and provides novel insights into future researchdirections for clinical treatment.展开更多
Summary: By culturing bone marrow mesenchymal stem cells of rabbits with fibrin glue in vitro, the biocompatibility of fibrin glue was investigated to study whether this material can be used as scaffolds in bone tissu...Summary: By culturing bone marrow mesenchymal stem cells of rabbits with fibrin glue in vitro, the biocompatibility of fibrin glue was investigated to study whether this material can be used as scaffolds in bone tissue engineering. After 2-months old New Zealand rabbits had been anesthetized, about 4-6 ml of bone marrow were aspirated from rabbit femoral trochanter. The monocytes suspension was aspirated after bone marrow was centrifuged with lymphocyte separating medium and cultured primarily. Then the cells were divided into two groups: one was cultured with complete medium and the other with induced medium. The cells of the two groups were collected and inoculated to the culture plate containing fibrin glue. In the control group, cells were inoculated without fibrin glue. The implanted cells and materials were observed at different stages under a phase-contrast microscope and scanning electron microscope. MTT and alkaline phosphatase (ALP) were measured. Bone marrow mesenchymal stem cells grew on the surface of fibrin glue and adhered to it gradually. Cells light absorption value (A value) and the ALP content showed no significant difference. Fibrin glue had no inhibitory effect on cell morphology, growth, proliferation and differentiation. It has good biocompatibility and can be used as scaffold materials for bone marrow mesenchymal stem cells in bone tissue engineering.展开更多
Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of al...Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network.Alginate nanobeads developed by calcium cross-linking yielded a size of 200e500 nm.The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus.Characterization of the in situ injectable hydrogel was done by SEM,FTIR and Rheometer.The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering.Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment,proliferation and infiltration within the hydrogel similar to fibrin gel.The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration.展开更多
Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth...Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue,its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors.We herein sought to develop a bioactive bioink composed of i-PRF,alginate and gelatin capable of promoting the regeneration of the oral soft tissue.This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs.Importantly,we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF.The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts.When deployed in vivo in nude mice,this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity.Together,these data thus confirmthe successful production of an i-PRF-containing bioink,which is suitable for the individualized promotion of the regeneration of oral soft tissue.展开更多
基金National Natural Science Foundation of China(No.50973082)
文摘The keratoprosthesis(KPro;artificial cornea)is a special refractive device to replace human cornea by using heterogeneous forming materials for the implantation into the damaged eyes in order to obtain a certain vision.The main problems of artificial cornea are the biocompatibility and stability of the tissue particularly in penetrating keratoplasty.The current studies of tissue-engineered scaffold materials through comprising composites of natural and synthetic biopolymers together have developed a new way to artificial cornea.Although a wide agreement that the long-term stability of these devices would be greatly improved by the presence of cornea cells,modification of keratoprosthesis to support cornea cells remains elusive.Most of the studies on corneal substrate materials and surface modification of composites have tried to improve the growth and biocompatibility of cornea cells which can not only reduce the stimulus of heterogeneous materials,but also more importantly continuous and stable cornea cells can prevent the destruction of collagenase.The necrosis of stroma and spontaneous extrusion of the device,allow for maintenance of a precorneal tear layer,and play the role of ensuring a good optical surface and resisting bacterial infection.As a result,improvement in corneal cells has been the main aim of several recent investigations;some effort has focused on biomaterial for its well biological properties such as promoting the growth of cornea cells.The purpose of this review is to summary the growth status of the corneal cells after the implantation of several artificial corneas.
基金supported by Education Department of Shaanxi Provincial Government(No.YJSZG2023134)Nanjing Medical University Fan Daiming Research Funds for Holistic Integrative Medicine.
文摘Tissue engineering technology has advanced rapidly in recent years, offering opportunities to construct biologicallyactive tissues or organ substitutes to repair or even enhance the functions of diseased tissues and organs.Tissue-engineered scaffolds rebuild the extracellular microenvironment by mimicking the extracellular matrix.Fibrin-based scaffolds possess numerous advantages, including hemostasis, high biocompatibility, and gooddegradability. Fibrin scaffolds provide an initial matrix that facilitates cell migration, differentiation, proliferation,and adhesion, and also play a critical role in cell-matrix interactions. Fibrin scaffolds are now widelyrecognized as a key component in tissue engineering, where they can facilitate tissue and organ defect repair.This review introduces the properties of fibrin, including its composition, structure, and biology. In addition, themodification and cross-linking modes of fibrin are discussed, along with various forms commonly used in tissueengineering. We also describe the biofunctionalization of fibrin. This review provides a detailed overview of theuse and applications of fibrin in skin, bone, and nervous tissues, and provides novel insights into future researchdirections for clinical treatment.
文摘Summary: By culturing bone marrow mesenchymal stem cells of rabbits with fibrin glue in vitro, the biocompatibility of fibrin glue was investigated to study whether this material can be used as scaffolds in bone tissue engineering. After 2-months old New Zealand rabbits had been anesthetized, about 4-6 ml of bone marrow were aspirated from rabbit femoral trochanter. The monocytes suspension was aspirated after bone marrow was centrifuged with lymphocyte separating medium and cultured primarily. Then the cells were divided into two groups: one was cultured with complete medium and the other with induced medium. The cells of the two groups were collected and inoculated to the culture plate containing fibrin glue. In the control group, cells were inoculated without fibrin glue. The implanted cells and materials were observed at different stages under a phase-contrast microscope and scanning electron microscope. MTT and alkaline phosphatase (ALP) were measured. Bone marrow mesenchymal stem cells grew on the surface of fibrin glue and adhered to it gradually. Cells light absorption value (A value) and the ALP content showed no significant difference. Fibrin glue had no inhibitory effect on cell morphology, growth, proliferation and differentiation. It has good biocompatibility and can be used as scaffold materials for bone marrow mesenchymal stem cells in bone tissue engineering.
基金One of the authors R.Jayakumar is grateful to the Department of Biotechnology(DBT),India,for providing fund under the scheme of Nanoscience and Nanotechnology Program(Ref.No.BT/PR 13585/NNT/28/474/2010)One of the author S.Deepthi is thankful to the Council of Scientific and Industrial Research for supporting financially under the CSIR-SRF award no:9/963(0034)2K13-EMR-I.
文摘Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network.Alginate nanobeads developed by calcium cross-linking yielded a size of 200e500 nm.The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus.Characterization of the in situ injectable hydrogel was done by SEM,FTIR and Rheometer.The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering.Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment,proliferation and infiltration within the hydrogel similar to fibrin gel.The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration.
基金supported by the National Key Research and Development Program of China(2017YFA0701302,PKUSS20200113).
文摘Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue,its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors.We herein sought to develop a bioactive bioink composed of i-PRF,alginate and gelatin capable of promoting the regeneration of the oral soft tissue.This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs.Importantly,we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF.The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts.When deployed in vivo in nude mice,this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity.Together,these data thus confirmthe successful production of an i-PRF-containing bioink,which is suitable for the individualized promotion of the regeneration of oral soft tissue.
