Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left ...Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left behind post-operatively, which reveals the surgeon’s line of invasive incision. This confers on the patient an adverse psychological reminder and burden for the rest of his/her life. Most patients cannot afford corrective plastic surgery to ameliorate this skin defect. This paper seeks to ask whether biomedical scientists could play a role in arriving at a more pleasing cosmetic result, using a simple cell culture procedure of isolating un-manipulated autologous primary epidermal and dermal cells from a small skin tissue segment in close proximity to the surgeon’s incision line.展开更多
AIM:To study the biological function of transforming growth factor a1(TGF-a 1),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF) during the process of osteoblasts allografts to accelerate f...AIM:To study the biological function of transforming growth factor a1(TGF-a 1),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF) during the process of osteoblasts allografts to accelerate fracture healing of osteoporosis rats.METHODS: Set up the fracture models of osteoporosis rats and do osteoblasts grafts; during different periods in the process of fracture heal ing by using immunnohistochemistry to detect the expression of TGF-a1,VEGF,bFG F and in situ hybridization to study the expression of bFGFmRNA,VEGFmRNA,TGF-a 1 mRNA,also using image analysis to deal with it.RESULTS:In experimental group, Osteoblasts can survive, even proliferate in fracture areas;VEGF,bFGF could be s een positive after 7 days of after osteoblast grafts,and there have the highest quantities about 14 days of post-transplantation.TGF-a1 could be seen positiv e after 3 days of post-transplantation,and there have the highest quantities ab out 7-10 days of post-transplantation.However there are not obviously high qua ntities in control.CONCLUSION:Osteoblasts grafts enhance bone fracture healing of osteoporosis rats.TGF-a1, VEGF and bFGF play very important roles in accel erating fracture healing of osteoporosis rats.展开更多
文摘Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left behind post-operatively, which reveals the surgeon’s line of invasive incision. This confers on the patient an adverse psychological reminder and burden for the rest of his/her life. Most patients cannot afford corrective plastic surgery to ameliorate this skin defect. This paper seeks to ask whether biomedical scientists could play a role in arriving at a more pleasing cosmetic result, using a simple cell culture procedure of isolating un-manipulated autologous primary epidermal and dermal cells from a small skin tissue segment in close proximity to the surgeon’s incision line.
文摘AIM:To study the biological function of transforming growth factor a1(TGF-a 1),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF) during the process of osteoblasts allografts to accelerate fracture healing of osteoporosis rats.METHODS: Set up the fracture models of osteoporosis rats and do osteoblasts grafts; during different periods in the process of fracture heal ing by using immunnohistochemistry to detect the expression of TGF-a1,VEGF,bFG F and in situ hybridization to study the expression of bFGFmRNA,VEGFmRNA,TGF-a 1 mRNA,also using image analysis to deal with it.RESULTS:In experimental group, Osteoblasts can survive, even proliferate in fracture areas;VEGF,bFGF could be s een positive after 7 days of after osteoblast grafts,and there have the highest quantities about 14 days of post-transplantation.TGF-a1 could be seen positiv e after 3 days of post-transplantation,and there have the highest quantities ab out 7-10 days of post-transplantation.However there are not obviously high qua ntities in control.CONCLUSION:Osteoblasts grafts enhance bone fracture healing of osteoporosis rats.TGF-a1, VEGF and bFGF play very important roles in accel erating fracture healing of osteoporosis rats.
