Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically li...Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.展开更多
The point spread function(PSF)caused by a wavefront coding system with a cubic phase mask has big side-lobes which leads to bad image restoration.This paper proposes a novel apodized cubic phase mask to suppress the s...The point spread function(PSF)caused by a wavefront coding system with a cubic phase mask has big side-lobes which leads to bad image restoration.This paper proposes a novel apodized cubic phase mask to suppress the side-lobes of the PSF.Simulated annealing algorithm is used to optimize the cubic and the truncation parameter of the phase mask.The system with the novel phase mask has better performance in the modulation transfer function(MTF)especially in low-and-medium spatial frequency region.The simulation results show that the restored images with the novel phase mask are superior to the one with the classic cubic phase mask in contrast and ringing effect.The experimental results show that the side-lobes of the PSF are suppressed by using the apodized cubic phase mask.展开更多
This article presents a 2017 LiDAR-DEM guided 1-m resolution examination of field-surveyed elevation and soil property variations (5 × 5 m spacings) conducted in 1977 across a hummocky New Brunswick field used fo...This article presents a 2017 LiDAR-DEM guided 1-m resolution examination of field-surveyed elevation and soil property variations (5 × 5 m spacings) conducted in 1977 across a hummocky New Brunswick field used for potato production. This examination revealed that the field incurred minor elevation differences likely due to upslope erosion as revealed through increasing Sand % and CF % with increasing elevation, and increasing Silt % along low-lying areas. Soil moisture, field capacity, permanent wilting and nitrate nitrogen (NO<sub>3</sub>-N) also increased at downslope locations. Directly as well as indirectly, soil pH, ammonium nitrogen (NH<sub>4</sub>-N), Caesium<sup>137</sup> (Cs<sup>137</sup>) and Mehlich-3 extracted Ca, Mg, K, Fe, Mn, Cu, and Zn were likewise affected by topographic location. Factor analyzing these variables led to: 1) a Soil Loss Factor that captured 24% of the textural variations;2) a Soil-Cropping Factor accounting for 16% of the N, P, K, Ca, Mg, Mn variations;3) a Soil Organic Matter (SOM) Factor relating 9% of the in-field variations for SOM, Fe, Zn, Cu to via organo-metal complexation and low NO<sub>3</sub>-N retention. Many of the topographic variations increased or decreased with the metric DEM-projected depth-to-water index (DTW) index. This index was set to 0 along DEM-derived flow channels with minimum upslope flow-accumulation areas of 0.1, 0.25, 0.5, 1 or 4 ha. Among these, the DTW > 4 ha threshold was useful for reproducing the textural variations, while the DTW > 0.25 ha threshold assisted in capturing trends pertaining to moisture retention and elemental concentrations.展开更多
A concept of divergence angle of light beams(DALB)is proposed to analyze the depth of field(DOF)of a 3D light-field display system.The mathematical model between DOF and DALB is established,and the conclusion that DOF...A concept of divergence angle of light beams(DALB)is proposed to analyze the depth of field(DOF)of a 3D light-field display system.The mathematical model between DOF and DALB is established,and the conclusion that DOF and DALB are inversely proportional is drawn.To reduce DALB and generate clear depth perception,a triple composite aspheric lens structure with a viewing angle of 100°is designed and experimentally demonstrated.The DALB-constrained 3D light-field display system significantly improves the clarity of 3D images and also performs well in imaging at a 3D scene with a DOF over 30 cm.展开更多
基金partially supported by the Gordon and Betty Moore Foundation Grant No.5722
文摘Structured illumination microscopy(SIM)is one of the most widely applied wide field super resolution imaging techniques with high temporal resolution and low phototoxicity.The spatial resolution of SIM is typically limited to two times of the diffraction limit and the depth of field is small.In this work,we propose and experimentally demonstrate a low cost,easy to implement,novel technique called speckle structured illumination endoscopy(SSIE)to enhance the resolution of a wide field endoscope with large depth of field.Here,speckle patterns are used to excite objects on the sample which is then followed by a blind-SIM algorithm for super resolution image reconstruction.Our approach is insensitive to the 3D morphology of the specimen,or the deformation of illuminations used.It greatly simplifies the experimental setup as there are no calibration protocols and no stringent control of illumination patterns nor focusing optics.We demonstrate that the SSIE can enhance the resolution 2–4.5 times that of a standard white light endoscopic(WLE)system.The SSIE presents a unique route to super resolution in endoscopic imaging at wide field of view and depth of field,which might be beneficial to the practice of clinical endoscopy.
文摘The point spread function(PSF)caused by a wavefront coding system with a cubic phase mask has big side-lobes which leads to bad image restoration.This paper proposes a novel apodized cubic phase mask to suppress the side-lobes of the PSF.Simulated annealing algorithm is used to optimize the cubic and the truncation parameter of the phase mask.The system with the novel phase mask has better performance in the modulation transfer function(MTF)especially in low-and-medium spatial frequency region.The simulation results show that the restored images with the novel phase mask are superior to the one with the classic cubic phase mask in contrast and ringing effect.The experimental results show that the side-lobes of the PSF are suppressed by using the apodized cubic phase mask.
文摘This article presents a 2017 LiDAR-DEM guided 1-m resolution examination of field-surveyed elevation and soil property variations (5 × 5 m spacings) conducted in 1977 across a hummocky New Brunswick field used for potato production. This examination revealed that the field incurred minor elevation differences likely due to upslope erosion as revealed through increasing Sand % and CF % with increasing elevation, and increasing Silt % along low-lying areas. Soil moisture, field capacity, permanent wilting and nitrate nitrogen (NO<sub>3</sub>-N) also increased at downslope locations. Directly as well as indirectly, soil pH, ammonium nitrogen (NH<sub>4</sub>-N), Caesium<sup>137</sup> (Cs<sup>137</sup>) and Mehlich-3 extracted Ca, Mg, K, Fe, Mn, Cu, and Zn were likewise affected by topographic location. Factor analyzing these variables led to: 1) a Soil Loss Factor that captured 24% of the textural variations;2) a Soil-Cropping Factor accounting for 16% of the N, P, K, Ca, Mg, Mn variations;3) a Soil Organic Matter (SOM) Factor relating 9% of the in-field variations for SOM, Fe, Zn, Cu to via organo-metal complexation and low NO<sub>3</sub>-N retention. Many of the topographic variations increased or decreased with the metric DEM-projected depth-to-water index (DTW) index. This index was set to 0 along DEM-derived flow channels with minimum upslope flow-accumulation areas of 0.1, 0.25, 0.5, 1 or 4 ha. Among these, the DTW > 4 ha threshold was useful for reproducing the textural variations, while the DTW > 0.25 ha threshold assisted in capturing trends pertaining to moisture retention and elemental concentrations.
基金supported by the National Key Research and Development Program of China(No.2021YFB3600504)the National Natural Science Foundation of China(Nos.62175015 and 62075016)。
文摘A concept of divergence angle of light beams(DALB)is proposed to analyze the depth of field(DOF)of a 3D light-field display system.The mathematical model between DOF and DALB is established,and the conclusion that DOF and DALB are inversely proportional is drawn.To reduce DALB and generate clear depth perception,a triple composite aspheric lens structure with a viewing angle of 100°is designed and experimentally demonstrated.The DALB-constrained 3D light-field display system significantly improves the clarity of 3D images and also performs well in imaging at a 3D scene with a DOF over 30 cm.
