Genotypic Differences in Growth and Physiological Responses to Transplanting and Direct Seeding Cultivation in Rice

The field experiments were conducted to investigate the growth and physiological responses of six super hybrid rice combinations to two planting methods, transplanting （TP） and direct seeding （DS） during 2006-2007 and 2007-2008. The 1000-grain weight and number of tillers per plant at the early growth stage, the maximum quantum yield of PSII （Fv/Fm） and transpiration rate （Tr） were higher in DS plants than in TP ones, whereas the grain yield, number of panicles per square meter, seed setting rate, net photosynthetic rate （Po） and stomatal conductance were lower in DS plants. However, little difference was detected in number of grains per panicle, stem （shoot） and leaf weight between the combinations in the two planting methods. The responses of plant growth and physiological traits to planting method differed greatly among the six combinations. In both planting methods, Chouyou 58 and Yongyou 6 had the highest and lowest panicle biomass and Pn, respectively. The higher yield of Chunyou 58 was associated with more numbers of panicles per square meter and grains per panicle in both planting methods. The results indicate that lower grain yield in DS relative to TP is attributed to more excessive tillers at the early stage, lower leaf biomass and photosynthetic rate at the late stage.

Effect of wide-narrow row arrangement in mechanical pot-seedling transplanting and plant density on yield formation and grain quality of japonica rice

Mechanical pot-seedling transplanting is an innovatively developed transplanting method that has the potential to replace mechanical carpet-seedling transplanting. However, the initial pot-seedling transplanting machine lacked optimized density spacing and limited yield potential for japonica rice. Therefore, ascertaining the optimized density by wide-narrow rows and the appropriate transplanting method for yield formation and grain quality of japonica rice is of great importance for high-quality rice production. Field experiments were conducted using two japonica rice cultivars Nanjing 9108 and Nanjing 5055 under three transplanting methods in 2016 and 2017: mechanical pot-seedling transplanting with wide-narrow row(K, average row spacing of 30 cm);equidistant row(D, 33 cm×12 cm);and mechanical carpet-seedling transplanting(T, 30 cm×12.4 cm). In addition, five different density treatments were set in K(K1–K5, from 18.62×10~4 to 28.49×10~4 hills ha^(–1)). The results showed that the highest yield was produced by a planting density of 26.88×104 hills ha^(–1) in mechanical pot-seedling transplanting with wide-narrow row with a greater number of total spikelets that resulted from significantly more panicles per area and slightly more grain number per panicle, as compared with equidistant row, and yield among density in wide-narrow row showed a parabolic trend. Compared with mechanical carpet-seedling transplanting, the treatment of the highest yield increased yield significantly, which was mainly attributed to the larger sink size with improved filled-grain percentage and grain weight, higher harvest index, and increased total dry matter accumulation, especially the larger amount accumulated from heading stage to maturity stage. With the density in wide-narrow row decreasing, processing quality, appearance quality, and nutrition quality were all improved, whereas amylose content and the taste value were decreased. Compared with mechanical carpet-seedling transplanting, mechanical pot-seedling transplanting improved processing quality and nutrition quality, but decreased amylose content and deteriorated appearance quality. These results suggested that mechanical pot-seedling transplanting with wide-narrow row coupling produced a suitable planting density of 26.88×10~4 hills ha^(–1) and may be an alternative approach to improving grain yield and quality for japonica rice.

Comparison of agronomic performance between inter-sub-specific hybrid and inbred japonica rice under different mechanical transplanting methods

Mechanical transplanting has been applied to rice cultivation to save labor costs and ease labor shortages in Asian countries, especially in China. However, little information is available related to the characteristics of agronomic performance when comparing inter-sub-specific hybrid rice(IHR) and inbred japonica rice(IJR) under mechanical transplanting method. In 2013 and 2014, field experiments were conducted using IHR(Yongyou 2640) and IJR(Wuyunjing 24) under two cultivation patterns, that is, pot seedlings mechanically transplanted(PS) and carpet seedlings mechanically transplanted(CS). Grain yield, yield components, leaf area index(LAI), leaf area duration(LAD), aboveground biomass, crop growth rate(CGR), nitrogen(N) uptake, and N accumulation were investigated. When compared with CS, PS displayed significantly increased grain yield for both varieties because the larger sink size allowed higher N accumulation from panicle initiation to maturity. Moreover, total aboveground biomass under PS increased significantly compared with that under CS; that is, higher photosynthetic productivity resulted from a greater LAI and higher LAD during the grain filling stage. Higher N absorption capacity in the middle and late growth periods resulted in significantly enhanced total N uptake under PS. When compared with IJR for both treatments, IHR generated 75.2% more grain yield. However, the characteristics creating high yield of IHR were different from those of IJR. Greater aboveground biomass production as well as higher N uptake and accumulation created higher grain yield in IHR than in IJR. These results suggest higher yield could be achieved using PS with IHR, attributing to exploit both yield superiority and productive potential.

Reducing environmental risk of nitrogen by popularizing mechanically dense transplanting for rice production in China

The high nitrogen(N)application rates typically used in Chinese cropping systems have led to diminishing returns for yields and have also imposed substantial environmental costs.Here,we estimate that the annual N loss from rice production in China reached approximately 2.6×109 kg from 2011 to 2015,and we demonstrate that adoption of the mechanically dense transplanting technique by producers is an effective method to reduce N loss from rice cropping systems without suffering a yield penalty.

Nitrogen Deep Placement Combined with Straw Mulch Cultivation Enhances Physiological Traits,Grain Yield and Nitrogen Use Efficiency in Mechanical Pot-Seedling Transplanting Rice

To assess the effects of straw return coupled with deep nitrogen(N)fertilization on grain yield and N use efficiency(NUE)in mechanical pot-seedling transplanting(MPST)rice,the seedlings of two rice cultivars,i.e.,Yuxiangyouzhan and Wufengyou 615 transplanted by MPST were applied with N fertilizer at 150 kg/hm2 and straw return at 6 t/hm2 in early seasons of 2019 and 2020.The experiment comprised of following treatments:CK(no fertilizer and no straw return),MDS(deep N fertilization and straw return),MBS(broadcasting fertilizer and straw return),MD(deep N fertilization without straw return),MB(broadcasting fertilizer without straw return).Results depicted that the MDS treatment significantly increased the rice yield by 41.69%-72.22%due to total above-ground biomass,leaf area index and photosynthesis increased by 54.70%-55.80%,38.52%-52.17%and 17.89%-28.40%,respectively,compared to the MB treatment.In addition,the MDS treatment enhanced the total N accumulation by 37.74%-43.69%,N recovery efficiency by 141.45%-164.65%,N agronomic efficiency by 121.76%-134.19%,nitrate reductase by 46.46%-60.86%and glutamine synthetase by 23.56%-31.02%,compared to the MB treatment.The average grain yield and NUE in both years for Yuxiangyouzhan were higher in the MDS treatment than in the MD treatment.Hence,deep N fertilization combined with straw return can be an innovative technique with improved grain yield and NUE in MPST in South China.

Compact plant type rice has higher lodging and N resistance under machine transplanting

To identify the major factors that contribute to the difference in lodging among different rice varieties under machine transplanting and their responses to nitrogen(N),field experiments were conducted at Danyang County(a representative eco-site of the Lower Yangtze River)in Jiangsu Province,China in 2017 and 2018,22 hybrid indica varieties(HIs),22 inbred japonica varieties(IJs)and two indica japonica hybrid varieties(IJHs)were transplanted by machine with three N rates(N0,N150 and N300,0,150 and 300 kg ha–1,respectively).Lodging-related physical parameters,morphological characteristics and apparent transport rates of dry matter were examined.Significant difference in yield was observed among different types of rice,and followed by IJs<HIs<IJHs.The average lodging index(LI)of hybrid varieties(HIs and IJHs)was higher than that of the inbred varieties(IJs)with higher plant height;moreover,lower apparent export rate of dry matter resulted lower LI in IJHs than in HIs.The HIs had a large difference in the LI,which came from the difference in bending stress(BS)induced by the difference in the apparent export rate of dry matter,varieties with lower leaf angle of upper three leaves possess strong lodging resistance capacity;however,the gap among the IJs was due to the difference in the cross section modulus(Z).The LI in the IJs or IJHs increased slightly with the increased N application,and there was no lodging incidence under the high N level,which was due to the low leaf angle and barely changed under high N;there was a significant interaction between varieties(HIs)and N rates in lodging rate and LI,varieties with lower leaf angle of upper three leaves were resistant to high N.These results suggest that compact plant type rice has higher lodging and N resistance at machine-transplanting method.

Critical Yield Factors for Achieving High Grain Yield in Early-Season Rice Grown under Mechanical Transplanting Conditions

Double-season rice cropping systems occupy a large portion of the rice production area in southern China.Because the problem of insufficient labor,mechanical transplanting(in contrast to the manual transplanting)was become more attractive in double-season rice system.However,the decisive yield factors which resulting in high grain yield of early-season rice are unclear under mechanical-transplanted conditions.In present study,the field experiments were conducted in the early season in 2017 and repeated in 2018 in Santang Town,Hunan Province,China.Ten early season rice cultivars(Zhuliangyou 819,Lingliangyou 268,Lingliangyou 104,Luliangyou 996,Xiangzaoxian 24,Xiangzaoxian 32,Xiangzaoxian 45,Xiangzaoxian 42,Zhongjiazao 17,and Zhongzao 39)were used as materials in this study.The difference in grain yield and closely-related agronomic and physiological traits of ten tested cultivars were compared.The range of yields(t ha^(–1) at 86%dry matter)in 2017 was 6.2 to 8.7(mean 7.8)and in 2018 was 6.5 to 8.4(mean 7.8).Grain weight and pre-heading biomass accumulation had potent significant positive correlations with the grain yield.The greater pre-heading biomass accumulation was major attributed to higher apparent radiation use efficiency.Our results suggested that early-season rice cultivars to achieve the high grain yield in mechanical-transplanted conditions depends on apparent radiation use efficiency in the pre-heading period and higher grain weight.

Effect of Nitrogen Applied Before Transplanting on NUE in Rice

Nitrogen （N） application before transplanting, where N fertilizers are applied in seedling-bed and carried to the paddy field with seedlings, is a novel method proposed in this article aiming for improving nitrogen utilization efficiency （NUE） in rice. The effect of this method on mineral N distribution in the rhizosphere soil was investigated in a field experiment with a japonica variety, Ningjing 2, in seasons of 2004 and 2005. There were four levels of N applied 16 h before transplanting： zero N （NO）, 207 kg ha^-1 （NL）, 310.5 kg ha^-1 （NM）, and 414 kg ha^-1 （NH）. The result indicated that N fertilizer before transplantation had positive effect of increasing mineral N content in the rhizosphere soil of rice. Generally, N content in the rhizosphere soil of rice tended to increase with the amount of N fertilizer before transplanting, with the NH treatment having the largest effect. Additionally, N fertilizer before transplanting had significant influence on rice NUE and grain yield. Compared with other treatments, the NM treatment showed the largest influence, with basal-tillering NUE, total NUE, and grain yield being 15%, 12%, and 529.5 kg ha^-1 higher than those of NO treatment. This result indicated that N fertilizer before transplantation had positive effect on mineral N distribution in the rhizosphere soil of rice, thus improving NUE and grain yield.

Evaluating the Optimum Transplanting Time for Different Coarse Rice Genotypes under Semi-Arid Conditions of Faisalabad

Continuously changing climate and availability of different rice genotypes make it necessary to find optimum time of sowing as well as suitable variety for cultivation to get maximum productivity under a specific set of climatic conditions. A field study was carried out to search out the suitable rice transplanting time for four different coarse genotypes under the semi-arid environment of Faisalabad. The experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad and was laid out in randomized complete block design (RCBD) with split plot arrangement keeping transplanting time in main plots while rice genotypes in subplots. Variability among treatments was measured by Fisher’s ANOVA (P ≤ 5%) and LSD test was applied to compare the differences among treatments’ means. The ANOVA indicated statistically significant differences among genotypes as well as transplanting dates irrespective of all studied traits while interactive effects of both were found to be non-significant. NIBGE-1 performed best with maximum paddy yield of 6.05 t/ha while KSK-434 performed poor with paddy yield of 2.78 t/ha. Increased paddy yield and yield related parameters of all rice genotypes were recorded where transplantation was done on 25th of June. Generally, paddy yield decreased with delaying the transplanting time. The results suggested that NIBGE-1 can perform better under the semi-arid conditions of Faisalabad and last week of June might be the optimum time for nursery transplantation. It can also be further elucidated that late transplanting causes yield reduction which could not be recommended among farmers.

Growth and yield of rice as affected by transplanting dates and seedlings per hill under high temperature of Dera Ismail Khan, Pakistan

Studies were initiated for two consecutive years to find out the effect of time of transplanting and seedlings hill?1 on the productivity of rice in Dera Ismail Khan district of North West Frontier Province (NWFP), Pakistan. The experiment was laid out in a randomized complete block design with split plot arrangements. Main plots consisted of four transplanting dates viz. 20th and 27th of June and 4th and 11th of July while sub-plots contained 1, 2, 3 or 4 seedlings hill?1. Among transplanting dates, June 20th planted crop gave highest paddy yield and net return with 1 seedling hill–1. It explains that the use of more seedlings hill?1 not only adds to cost but is also a mere wastage of natural resources. Based on research findings, we conclude that the use of 1 seedling hill?1 is most appropriate for timely sowing otherwise 4 seedlings hill–1 should be used to compensate for the yield gap in late transplanted rice.

Mechanized Transplanting of Rice(Oryza sativa L.)in Nonpuddled and No-Till Conditions in the Rice-Wheat Cropping System in Haryana,India

The common practice of establishing rice in the rice-wheat system in India is manual transplanting of seedlings in the puddled soil. Besides being costly, cumbersome, and time consuming, puddling results in degradation of soil and the formation of a hard pan, which impedes root growth of subsequent upland crops. In addition, decreased availability and increasing cost of labor have increased the cost of rice cultivation through conventional methods. Because of these concerns, there is a need for mechanized transplanting of rice which is less labor-intensive and can ensure optimum plant population under nonpuddled and/or no-till conditions. A large number of on-farm trials were conducted at farmers’ fields in Haryana, India, from 2006 to 2010 to evaluate the performance of the mechanical transplanted rice (MTR) under nonpuddled and no-till situations as compared to conventional puddled transplant rice (CPTR). Compared with CPTR, nonpuddled MTR produced 3%-11% higher grain yield in different years. Rice cultivars, viz. HKR47, HKR127, PR113, PR114, PB1, PB1121, CSR30, and Arize6129, performed consistently better under nonpuddled MTR as compared to CPTR. Performance of different cultivars (PR113, PR114, HKR47, and Pusa 44) was also better under no-till MTR as compared to CPTR. The “basmati” cultivar CSR30 performed equally in no-till MTR and CPTR systems. The results of our study suggest that rice can be easily grown under nonpuddled and no-till conditions with yield advantages over the CPTR system. Even in the case of similar yield between CPTR and MTR systems, the MTR system will help in reducing labor requirement and ultimately, will increase overall profits to farmers.

Overlapped Tray Seedling Raising Model for Mechanical Transplanting of Rice

With the development of China's social economy,transfer of rural labors,and rise of labor costs,the rice production technologies are changing from manual operation to mechanical operation.The core of mechanical rice production is mechanical planting,while the key to mechanical planting is the seedling raising.On the basis of analyzing and studying the problems and experience of traditional mechanical transplanting of rice,a new overlapped tray seedling raising model was introduced.Main features of this model: substrate seedling raising and overlapped seedling tray.The seedling trays for seeding line are overlapped,and the overlapped trays are moved into the seedling room with controlled temperature and humidity.The temperature of the seedling room is controlled at 30-32℃,and the humidity of the seedling room is controlled at above 90%.After about 48 h,when the seedling height reaches 0.5 cm,move the seedling trays to the nursery.This model consists of one seeding center( seedling raising center) and N nurseries,to realize 1 + N seedling raising for mechanical transplanting of rice.One seeding center can provide seedlings for several hundred to several thousand hectares of mechanical transplanting of rice,and provide a new model for social services.This model could improve the quality of seedlings,increase the seedling survival rate by about 20%,reduce the seedling raising costs by 15%-20%,reduce the seedling raising risks,and greatly increase the utilization rate of the site and equipment of seedling raising center.

Rice Transplanting Mechanization in Bangladesh: Way to Make It Sustainable

“Rice transplanter” has brought a radical change in the method of rice seedlings transplanting in Bangladesh. The machine is gaining popularity with the increase of labor crisis during the peak period of transplanting, reduction time of in-between crops, rapid urbanization and industrialization, and changing socio-economic status of the farmers. Alternatively, the traditional way of rice transplanting is labor-intensive and involves high drudgery. Mechanical rice transplanter is cost-effective and operation-friendly. It is suitable to plant young seedlings maintaining plant to plant and line to line distance which plays an important role in increasing yield. Moreover, it is easy to take care of the crops planted in rows. It helps in maintaining soil physical properties and is considered to be better from a crop management and productivity point of view. In malignancy of having an edge over the traditional broadcasting, the adoption rate of mechanical transplanters is low due to high primary investment, lack of knowledge in growing mat type nursery, lack of repair and maintenance facility to the end-users, and unavailability of spare parts. Imparting these, available supply, entrepreneurship development, and encouraging custom hire services may be some of the practical results for adding the rice area under mechanical transplanting. This review summarizes the problems and prospects associated with seedling rice transplanting, technological gaps in the adoption of mechanical transplanters in a sustainable manner, and future ways to make transplanters more user-friendly and cost-effective.

Effect of Nitrogen and Density Interaction on Yield Formation of Late Japonica Rice Under Different Transplanting Dates

[Objectives]This study was conducted to clarify the appropriate nitrogen fertilizer amount and transplanting density that should be selected for different transplanting dates.[Methods]The effects of nitrogen fertilizer rate and transplanting density on the traits and yield of late japonica rice in the Jianghuai rice-wheat cropping area under different transplanting dates(transplanting at an appropriate time and delayed transplanting)were studied.[Results]The transplanting date significantly affected the heading,maturity and whole growth period of rice,and when the transplanting date of early-maturing late japonica rice was delayed by 15 d,the growth period was shortened by about 10 d.Nitrogen and density interaction obviously affected the dry matter accumulation and effective tillers of rice,and the dry matter production and effective tiller number of rice treated with B_(1)N_(2) were greater than other treatments.Under the timely transplanting date,the best nitrogen and density combination was 225-300 kg/hm^(2) and 250500 holes/hm^(2),and the nitrogen use efficiency was ideal.It suggests that under the condition of transplanting rice in a timely manner,applying nitrogen fertilizer at an appropriate rate while ensuring a reasonable population density is conducive to the coordination of the number of stems and tillers,the number of grains per panicle,etc.,thereby ensuring the best formation of yield factors in the middle and late stages of rice growth.[Conclusions]This study provides technical support and theoretical reference for reasonable nitrogen and density interaction of rice.

Why high grain yield can be achieved in single seedling machine-transplanted hybrid rice under dense planting conditions?

This study was conducted to identify the factors associated with high grain yield in single seedling machine-transplanted hybrid rice under dense planting conditions. Field experiments were done in Yong＇an Town, Hunan Province, China in 2015 and 2016. Two hybrid rice cultivars were grown under single seedling machine transplanting （SMT） and conventional machine transplanting （CMT） at a high planting density in each year. Grain yield and yield attributes were compared between SMT and CMT. Averaged across cultivars and years, grain yield was 12％ higher under SMT than under CMT. Plant height, basal stem width, and shoot and root dry weights were higher in seedlings for SMT than those for CMT. SMT had less maximum tiller number per m2 and consequently less panicle number per m2 than did CMT. Branch number per panicle, especially the secondary branch number per panicle, and spikelet number per cm of panicle length were more under SMT than under CMT, which resulted in more spikelet number per panicle under SMT than under CMT. SMT had higher or equal spikelet filling percentage than did CMT. The difference in grain weight between SMT and CMT was relatively small and inconsistent cross years. SMT had higher or equal total biomass and harvest index than did CMT. Dry weight per stem under SMT was heavier than that under CMT. Larger leaf area per stem was partly responsible for the heavier dry weight per stem under SMT than under CMT. Our study suggests that improvement in seedling quality, panicle size, and dry weight per stem are critical to the high grain yield in single seedling machine-transplanted hybrid rice under dense planting conditions.

Effects of soilless substrates on seedling quality and the growth of transplanted super japonica rice

This study was conducted to investigate the effects of soilless substrates of hydroponically grown long-mat seedlings （HLMS） on seedling quality and field growth characteristics of transplanted super japonica rice. A widely grown conventional super japonica rice cultivar （Wuyunjing 23） was selected as the test material. The effect of HLMS on seedling quality, mechanical transplantation quality, field growth characteristics, yield, and benefit-cost ratio were compared with seedlings grown in organic substrates and traditional nutritive soil, whJch was selected as the control. Root number, root twJstJng power and root activity of seedlings cultivated by HLMS were decreased compared to that of the organic substrates andcontrol. However, seedling root length as well as aboveground growth were increased compared to the organic substrates and control seed- lings. In the HLMS, the content of gibberellin acid （GA3） decreased while abscisic acid （ABA） content increased compared to that of the organic substrates and control seedlings. During the early stages after transplanting, the re-greening of HLMS was delayed compared to that of the organic substrates and control seedlings. Nevertheless, there were no significant differences in tiller dynamics and crop yield among the HLMS, organic substrates and control treatments. The effects of HLMS on seedling production were similar to those of the organic substrates and traditional nutritive soil in the present study, suggesting that HLMS have the potential to replace traditional nutritive soil in seedling production without decreasing crop yield. Finally, it is important to reduce organic substrates and topsoil dependence during rice seedling production and worthwhile to consider HLMS popularization and its application on a larger scale.

Correlation of Leaf and Root Senescence During Ripening in Dry Seeded and Transplanted Rice

Dry seeding is a resource-saving rice establishment method. With an equivalent yield, dry seeded flooded rice （DSR） has been considered as a replacement for traditional transplanted flooded rice （TFR）. However, the differences in leaf and root senescence during grain filling between DSR and TFR were seldom identified. In this study, the root length, root tip number and leaf senescence of rice varieties Huanghuazhan and Yangliangyou 6 during ripening were compared between DSR and TFR. Results showed that top three leaves in DSR had the characteristics of relatively lower SPAD value, lower N content and premature leaf senescence. In addition, both the total root length and total root tip number of DSR were significantly lower than those of TFR. In conclusion, premature and quick leaf senescence was related with inadequate root length and root tip number during ripening, which might result from the deficiency of nitrogen supply in DSR. Techniques on improving leaf nitrogen status and delaying the leaf senescence during grain-filling in DSR should be developed in future researches.

No-tillage effects on grain yield and nitrogen requirements in hybrid rice transplanted with single seedlings: Results of a long-term experiment

This study was conducted to determine whether,and if so how,the grain yield and nitrogen(N) requirements of hybrid rice transplanted as single seedlings are affected by no-tillage(NT) practices.A fixed field experiment was done at the Experimental Farm of Hunan Agricultural University in Changsha,Hunan Province,China,from 2004 to 2014.Grain yield and yield attributes(panicle number per m2,spikelet number per panicle,spikelet filling percentage,grain weight,total biomass,and harvest index) were evaluated as well as the N-use characteristics(total N uptake,internal N-use efficiency,and N requirements) of hybrid rice transplanted as single seedlings comparing NT with conventional tillage(CT).A significant finding was that there were no significant differences in grain yield,yield attributes,and N-use characteristics between CT and NT.Averaged across the 11 years,grain yield and N requirements were 9.51 t ha^(-1) and 20.2 kg t^(-1) under CT and 9.33 t ha^(-1) and 20.0 kg t^(-1) under NT,respectively.There were significant yearly variations in grain yield,yield attributes,and N-use characteristics observed under both CT and NT.The yearly variation in grain yield was related to simultaneous changes in spikelet number per panicle,grain weight,total biomass,and harvest index.Also,it was found that grain yield was positively correlated with internal N-use efficiency but negatively correlated with N requirements.It is concluded that grain yield and N requirements in hybrid rice when transplanted as single seedlings are not affected adversely by NT.The results of this study suggest that(1) compatible relationships among yield attributes can be established in hybrid rice that is transplanted as single seedlings,and(2) higher grain yield and higher N-use efficiency can be concurrently achieved in hybrid rice transplanted as single seedlings.

Temporal Dynamics of Antioxidant Defence System in Relation to Polyamine Catabolism in Rice under Direct-Seeded and Transplanted Conditions

Six rice cultivars viz. PR120, PR116, Feng Ai Zan, PR115, PAU201 and Punjab Mehak 1 under the direct-seeded and transplanted conditions were used to investigate the involvement of antioxidative defence system in relation to polyamine catabolism in temporal regulation of developing grains. Activities of ascorbate peroxidase （APx）, guaiacol peroxidase （GPx）, catalase （CAT）, superoxide dismutase （SOD）, polyamine oxidases （PAO） and contents of ascorbate, a-tocopherol, proline and polyamines increased gradually until mid-milky stage and then declined towards maturity stage under both planting conditions. The transplanted condition led to higher activities of antioxidative enzymes （APx, GPx and CAT） and contents of ascorbate, a-tocopherol and proline whereas the direct-seeded condition had elevated levels of PAO and SOD activities and contents of polyamines, lipid peroxide and hydrogen peroxide. Cultivars Feng Ai Zan and PR120 exhibited superior tolerance over other cultivars by accumulating higher contents of ascorbate, a-tocopherol and proline with increasing level of PAO and SOD activities under the direct-seeded condition. However, under the transplanted condition PR116 and PAU201 showed higher activities of antioxidative enzymes with decreasing content of lipid peroxide. Therefore, we concluded that under the direct-seeded condition, enhancements of polyamines content and PAO activity enabled rice cultivars more tolerant to oxidative stress, while under the transplanted condition, antioxidative defence with decreasing of lipid peroxide content was closely associated with the protection of grains by maintaining membrane integrity during rice grain filling. The results indicated that temporal dynamics of H2O2 metabolic machinery was strongly up-regulated especially at the mid-milky stage.

Development and Experiment of 2ZB-79 Shallow Rice Seedling Transplanter

Shallow planting of the rice seedlings is an important factor for getting high yield as it can increase the effective tillers on the lownode of the stem. The 2 ZB-79 shallow rice seedling transplanter combined the advantages of shallow planting of the seedling-casting rice transplanter and orderly planting of the traditional rice transplanter. The principle of this kind of machine is firstly to cut the standardization nursery rice seedlings with rug soil into many small pieces,and then to plant the small pieces composed of pot soil and some seedlings on it to the field surface in order,only shallowly planted on the very top part of paddy soil. Not only it can keep the performance of planting shallowly and orderly,but also simplify many mechanisms for transition,separation,and plantation of rice seedlings. It is a new type of rice seedling transplanter called laying-type up to now to get higher efficiency when working and higher yield for rice production. This paper will introduce its developing results and analyze the comparative experiments.