Herein we report a highly sensitive filter-less fluorescence detection method using an APD （avalanche photodiode）. Experimental measurements using the proposed APD-based highly sensitive fluorescence detection metho...Herein we report a highly sensitive filter-less fluorescence detection method using an APD （avalanche photodiode）. Experimental measurements using the proposed APD-based highly sensitive fluorescence detection method exhibits the sensing capability to detect an excitation light and a fluorescence light without band pass filter or grating. The principle of this APD-based highly fluorescence detection method is used the varying multiplication ratio that is decided by wavelength. The wavelength controls running distance of photo-excited carrier by absorption coefficients, and this element decide multiplication ratio on fixed high electrical field. In fluorescence detection, they use two types of light： excitation light and fluorescence light. These lights have different wavelengths and make different multiplication ratio as well. Thus this method can separate two types of light easily by using multiplication ratios of APD without band pass filters/gratings. In this experiment, the excitation light is LED （light emitting diode） and fluorescence light occurs from FITC （fluorescein isothiocyanate） with ethanol. The FITC concentration changes from 0.1 μmol/L to 10 mmol/L. In this measurement circuit, we employ APD （S2385）, power supply voltage, and pico ampere current meter. As a result, these lights are correctly separated by using multiplication ratio with calculation at every concentration FITCs.展开更多
滤膜堵塞型传感器可以半定量地评价油液的固体颗粒污染度,其关键元件为金属微孔滤膜.为了获得性价比较高的金属微孔滤膜,选择600目斜纹不锈钢丝网为镀覆基材,研究所选不锈钢丝网表面化学镀镍的工艺流程,通过扫描电镜（LEO1450VP）和能...滤膜堵塞型传感器可以半定量地评价油液的固体颗粒污染度,其关键元件为金属微孔滤膜.为了获得性价比较高的金属微孔滤膜,选择600目斜纹不锈钢丝网为镀覆基材,研究所选不锈钢丝网表面化学镀镍的工艺流程,通过扫描电镜（LEO1450VP）和能谱分析仪（INCA ENERGY 300）分析镀层成分、镀层与基材的结合情况以及微孔滤膜的微孔及丝径尺寸.结果表明,本研究工艺下制备的微孔滤膜镀层为低磷镀层,抗拉强度为400～700MPa,硬度为580HV,镀层与基体材料结合牢固.镀覆20min和30min后可获得微孔尺寸分别为14μm和10μm的微孔滤膜,且丝径镀覆厚度均匀一致,微孔大小也均匀一致.利用所研制的金属微孔滤膜制成滤膜堵塞型传感器,配制样液分别对该传感器的重复性精度和污染度检测精度进行检验,结果表明,微孔滤膜传感器具有较好的重复性精度,对油液中固体颗粒污染度等级的测定结果与实际值完全一致,因此所研制的金属微孔滤膜可以满足滤膜堵塞型传感器的使用要求.展开更多
文摘Herein we report a highly sensitive filter-less fluorescence detection method using an APD （avalanche photodiode）. Experimental measurements using the proposed APD-based highly sensitive fluorescence detection method exhibits the sensing capability to detect an excitation light and a fluorescence light without band pass filter or grating. The principle of this APD-based highly fluorescence detection method is used the varying multiplication ratio that is decided by wavelength. The wavelength controls running distance of photo-excited carrier by absorption coefficients, and this element decide multiplication ratio on fixed high electrical field. In fluorescence detection, they use two types of light： excitation light and fluorescence light. These lights have different wavelengths and make different multiplication ratio as well. Thus this method can separate two types of light easily by using multiplication ratios of APD without band pass filters/gratings. In this experiment, the excitation light is LED （light emitting diode） and fluorescence light occurs from FITC （fluorescein isothiocyanate） with ethanol. The FITC concentration changes from 0.1 μmol/L to 10 mmol/L. In this measurement circuit, we employ APD （S2385）, power supply voltage, and pico ampere current meter. As a result, these lights are correctly separated by using multiplication ratio with calculation at every concentration FITCs.
文摘滤膜堵塞型传感器可以半定量地评价油液的固体颗粒污染度,其关键元件为金属微孔滤膜.为了获得性价比较高的金属微孔滤膜,选择600目斜纹不锈钢丝网为镀覆基材,研究所选不锈钢丝网表面化学镀镍的工艺流程,通过扫描电镜（LEO1450VP）和能谱分析仪（INCA ENERGY 300）分析镀层成分、镀层与基材的结合情况以及微孔滤膜的微孔及丝径尺寸.结果表明,本研究工艺下制备的微孔滤膜镀层为低磷镀层,抗拉强度为400～700MPa,硬度为580HV,镀层与基体材料结合牢固.镀覆20min和30min后可获得微孔尺寸分别为14μm和10μm的微孔滤膜,且丝径镀覆厚度均匀一致,微孔大小也均匀一致.利用所研制的金属微孔滤膜制成滤膜堵塞型传感器,配制样液分别对该传感器的重复性精度和污染度检测精度进行检验,结果表明,微孔滤膜传感器具有较好的重复性精度,对油液中固体颗粒污染度等级的测定结果与实际值完全一致,因此所研制的金属微孔滤膜可以满足滤膜堵塞型传感器的使用要求.
