[Objectives]To establish HPLC finger-print of Marchantia convolute Gao et Chang,and to control the quality of medicinal materials. [Methods] Agilent ZORBAX SB-C18 chromatographic column( 4. 6 mm × 250 mm,5μm) wa...[Objectives]To establish HPLC finger-print of Marchantia convolute Gao et Chang,and to control the quality of medicinal materials. [Methods] Agilent ZORBAX SB-C18 chromatographic column( 4. 6 mm × 250 mm,5μm) was adopted,acetonitrile( B)-0. 1% phosphoric solution( D) was used as the mobile phase( gradient elution),and the detection was conducted at the wavelength of 230 nm and at the column temperature of 25℃. HPLC finger-print of herb materials in the upland-field was established,and 8 lots of medicinal materials were used for the similarity assessment to establish the finger-print. [Results] Theprecision,repeatability and stability RSD of HPLC finger-prints of the 8 lots of Marchantia convoluta Gao et Chang were all less than 3%,and their similarities were all greater than 0. 75; 6 common peaks were observed,and the control finger-print was also established. [Conclusions] With reliable data and simple and efficient manipulations,HPLC finger-print analysis can provide references for the quality assessment of Marchantia convoluta Gao et Chang.展开更多
基金Supported by National Natural Science Foundation of China(NSFC)(No.81260620)Zhuang Yao Medicines Collaborative Innovation Center[Gui JKY(2013)No.20]+2 种基金Guangxi Zhuang Yao Medicines Key Laboratory[Gui KJZ(2014)No.32]Guangxi Key Disciplines on Zhuang Medicine[Gui JKY(2013)No.16]Studies of Bagui Scholars on Innovation Theory and Efficacy of TCM
文摘[Objectives]To establish HPLC finger-print of Marchantia convolute Gao et Chang,and to control the quality of medicinal materials. [Methods] Agilent ZORBAX SB-C18 chromatographic column( 4. 6 mm × 250 mm,5μm) was adopted,acetonitrile( B)-0. 1% phosphoric solution( D) was used as the mobile phase( gradient elution),and the detection was conducted at the wavelength of 230 nm and at the column temperature of 25℃. HPLC finger-print of herb materials in the upland-field was established,and 8 lots of medicinal materials were used for the similarity assessment to establish the finger-print. [Results] Theprecision,repeatability and stability RSD of HPLC finger-prints of the 8 lots of Marchantia convoluta Gao et Chang were all less than 3%,and their similarities were all greater than 0. 75; 6 common peaks were observed,and the control finger-print was also established. [Conclusions] With reliable data and simple and efficient manipulations,HPLC finger-print analysis can provide references for the quality assessment of Marchantia convoluta Gao et Chang.
