Aiming to ensure the consistency of quality control of Traditional Chinese Medicines(TCMs),a combination method of high-performance liquid chromatography(HPLC),ultraviolet(UV),electrochemical(EC)was developed in this ...Aiming to ensure the consistency of quality control of Traditional Chinese Medicines(TCMs),a combination method of high-performance liquid chromatography(HPLC),ultraviolet(UV),electrochemical(EC)was developed in this study to comprehensively evaluate the quality of Antiviral Mixture(AM),and Comprehensive Linear Quantification Fingerprint Method(CLQFM)was used to process the data.Quantitative analysis of three active substances in TCM was conducted.A fivewavelength fusion fingerprint(FWFF)was developed,using second-order derivatives of UV spectral data to differentiate sample levels effectively.The combination of HPLC and UV spectrophotometry,along with electrochemical fingerprinting(ECFP),successfully evaluated total active substances.Ultimately,a multidimensional profiling analytical system for TCM was developed.展开更多
目的基于质量源于设计(quality by design,QbD)理念对油菜花粉颗粒进行制备工艺研究及质量控制。方法以油菜花粉颗粒成型率(%)、休止角(°)、平衡吸湿率(%)为评价指标,通过G1-熵权法组合赋权得到综合评价指标,单因素试验结合Box-Beh...目的基于质量源于设计(quality by design,QbD)理念对油菜花粉颗粒进行制备工艺研究及质量控制。方法以油菜花粉颗粒成型率(%)、休止角(°)、平衡吸湿率(%)为评价指标,通过G1-熵权法组合赋权得到综合评价指标,单因素试验结合Box-Behnken响应面法优化油菜花粉颗粒制备工艺。采用物理指纹图谱评价不同批次油菜花粉颗粒的物理质量一致性并测定临界相对湿度。结果油菜花粉颗粒最佳制备工艺为:淀粉、甘露醇作为填充剂,药辅比1∶2、乙醇浓度55%、辅料配比7∶3。10批油菜花粉颗粒物理指纹图谱相似度均大于0.99。结论基于QbD理念的油菜花粉颗粒制备工艺稳定可行,可用于油菜花粉颗粒的制备。展开更多
Using Dachengqi Tang(DCQT) as a model, high performance liquid chromatography(HPLC)fingerprints were applied to optimize machine extracting process with the Box–Behnken experimental design. HPLC fingerprints were...Using Dachengqi Tang(DCQT) as a model, high performance liquid chromatography(HPLC)fingerprints were applied to optimize machine extracting process with the Box–Behnken experimental design. HPLC fingerprints were carried out to investigate the chemical ingredients of DCQT; synthetic weighing method based on analytic hierarchy process(AHP) and criteria importance through intercriteria correlation(CRITIC) was performed to calculate synthetic scores of fingerprints; using the mark ingredients contents and synthetic scores as indicators, the Box–Behnken design was carried out to optimize the process parameters of machine decocting process under high pressure for DCQT. Results of optimal process showed that the herb materials were soaked for 45 min and extracted with 9 folds volume of water in the decocting machine under the temperature of 140 1C till the pressure arrived at 0.25 MPa;then hot decoction was excreted to soak Dahuang and Mangxiao for 5 min. Finally, obtained solutions were mixed, filtrated and packed. It concluded that HPLC fingerprints combined with the Box–Behnken experimental design could be used to optimize extracting process of traditional Chinese medicine(TCM).展开更多
基金This study was supported by the National Natural Science Foundation of China(No.81573586).
文摘Aiming to ensure the consistency of quality control of Traditional Chinese Medicines(TCMs),a combination method of high-performance liquid chromatography(HPLC),ultraviolet(UV),electrochemical(EC)was developed in this study to comprehensively evaluate the quality of Antiviral Mixture(AM),and Comprehensive Linear Quantification Fingerprint Method(CLQFM)was used to process the data.Quantitative analysis of three active substances in TCM was conducted.A fivewavelength fusion fingerprint(FWFF)was developed,using second-order derivatives of UV spectral data to differentiate sample levels effectively.The combination of HPLC and UV spectrophotometry,along with electrochemical fingerprinting(ECFP),successfully evaluated total active substances.Ultimately,a multidimensional profiling analytical system for TCM was developed.
文摘目的基于质量源于设计(quality by design,QbD)理念对油菜花粉颗粒进行制备工艺研究及质量控制。方法以油菜花粉颗粒成型率(%)、休止角(°)、平衡吸湿率(%)为评价指标,通过G1-熵权法组合赋权得到综合评价指标,单因素试验结合Box-Behnken响应面法优化油菜花粉颗粒制备工艺。采用物理指纹图谱评价不同批次油菜花粉颗粒的物理质量一致性并测定临界相对湿度。结果油菜花粉颗粒最佳制备工艺为:淀粉、甘露醇作为填充剂,药辅比1∶2、乙醇浓度55%、辅料配比7∶3。10批油菜花粉颗粒物理指纹图谱相似度均大于0.99。结论基于QbD理念的油菜花粉颗粒制备工艺稳定可行,可用于油菜花粉颗粒的制备。
基金financially supported by Longhua Medical Project (LYTD-14)the National Special Research Foundation of TCM (No.201007010)
文摘Using Dachengqi Tang(DCQT) as a model, high performance liquid chromatography(HPLC)fingerprints were applied to optimize machine extracting process with the Box–Behnken experimental design. HPLC fingerprints were carried out to investigate the chemical ingredients of DCQT; synthetic weighing method based on analytic hierarchy process(AHP) and criteria importance through intercriteria correlation(CRITIC) was performed to calculate synthetic scores of fingerprints; using the mark ingredients contents and synthetic scores as indicators, the Box–Behnken design was carried out to optimize the process parameters of machine decocting process under high pressure for DCQT. Results of optimal process showed that the herb materials were soaked for 45 min and extracted with 9 folds volume of water in the decocting machine under the temperature of 140 1C till the pressure arrived at 0.25 MPa;then hot decoction was excreted to soak Dahuang and Mangxiao for 5 min. Finally, obtained solutions were mixed, filtrated and packed. It concluded that HPLC fingerprints combined with the Box–Behnken experimental design could be used to optimize extracting process of traditional Chinese medicine(TCM).