Aflatoxin contamination and rancidity in locally processed commercial fish feeds and ingredients along value chains is a Public and Animal health hazard. The study established the level of aflatoxin contamination, per...Aflatoxin contamination and rancidity in locally processed commercial fish feeds and ingredients along value chains is a Public and Animal health hazard. The study established the level of aflatoxin contamination, peroxide value (PV), Anisidine value (AnV), and their associated factors at storage areas among farmers, processors, traders, factories, and landing sites in the Wakiso and Kampala districts. The value chain actors were purposively selected in a cross-sectional study based on access to the feed store and the use of locally processed commercial fish feeds on farms. Data collected were statistically analyzed in SPSS version 20. All the samples (45) were positive for aflatoxin contamination and PV with 51% (23/45) of samples being contaminated with “above acceptable” aflatoxin levels and 66.6% (30/45) of samples with “above acceptable” PV. The overall percentage of “above acceptable” AnV was 11/29 (37.9%). Samples from factories were within acceptable contamination levels. Multivariate logistic regression analysis revealed no significant difference between aflatoxin contamination, peroxide, and Anisidine value with storage factors for locally processed commercial fish feeds and ingredients. The study recommended the purchase of fish feeds from factories and a larger study on storage factors responsible for aflatoxin contamination and rancidity in fish feeds in Uganda.展开更多
PBDEs are widely used brominated flame retardant, which are increasingly reported in the environment. Concentrations of thirteen PBDEs in a large number of channel catfish and fish feeds collected from Hubei province ...PBDEs are widely used brominated flame retardant, which are increasingly reported in the environment. Concentrations of thirteen PBDEs in a large number of channel catfish and fish feeds collected from Hubei province of China were investigated in this study. A fast isotopic dilution GC-MS method was firstly developed to simultaneously determine thirteen PBDEs in channel catfish and fish feeds in this study, and especially for the first time PBDEs in fish feeds. Pressurized liquid extraction and multi-layer silica gel column chromatography cleanup were used, some important steps and crucial parameters were modified and intensified compared with other literatures, and GC and MS conditions were optimized. The limits of quantitation values of 0.25 - 5 μg·kg-1 wet weight in channel catfish were calculated for PBDEs;so did 1 - 20 μg·kg-1 wet weight in fish feeds. In addition, good repeatability and accuracy of the whole method were achieved. The established methods were therefore suitable for the simultaneous determinations of thirteen PBDEs in these samples at trace contamination levels. Using the established methods, PBDEs emerged in 10 of 180 channel catfish and 13 of 115 fish feeds, and the dominant PBDEs homologues were PBDE-28, PBDE-47, PBDE-100, PBDE-99, PBDE-154, PBDE-153 and PBDE-183. The detection rates of seven PBDEs congeners were in turn respectively 1.74%, 7.83%, 4.35%, 5.22%, 3.48%, 2.61% and 2.61% in fish feeds, and detection rates of seven PBDEs congeners were in turn respectively 1.11%, 3.89%, 2.22%, 1.67%, 1.11%, 1.11% and 1.11% in channel catfish. There was significant correlation in PBDEs between matching channel catfish and fish feeds (R2 = 0.742, P < 0.001, n = 30), suggesting that fish feeds contaminated with PBDEs possibly resulted in PBDEs residual in channel catfish through biological transfer.展开更多
In recent years, fish invasion has become one of the main reasons for the decline of native fish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated th...In recent years, fish invasion has become one of the main reasons for the decline of native fish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated the fish resources of the Chabalang Wetland (Lhasa, Tibet) during diff erent seasons in 2009 and 2013. Four hundred and twelve individuals were subsampled to estimate age, growth, and feeding habit of P . parva . Furthermore, food relationships between P . parva and the native Schizothoracinae fish were also examined. The results revealed a significant shift in species composition and community structure characterized by the disappearance of native fish and outbreak of non-native fish. The percentage of nonnative P . parva in the fish collections significantly increased from 33.64% in 2009 to 64.08% in 2013. The standard length (SL) ranged from 22.00 to 78.71 mm, and their age was 1–5 a. The von Bertalanff y function was used to model the observed length-at-age data as L t =112.19(1–e^-0.1495 ( t +0.8012)) for females and as L t =123.12 (1–e^-0.1500 ( t +0.7132)) for males. The results indicated that P . parva in Tibet has lower growth and mortality rates compared with that from the native ranges. Ninety-seven prey taxa belonging to 9 prey categories were identified in the gut of 38 P . parva . P . parva can be considered a generalized and opportunistic predator, competing with the native fish, especially Schizothorax o’connori , Schizopygopsis younghusbandi younghusbandi , and Ptychobarbus dipogon , for Bacillariophyta and Chironomid larvae. This is an important reason for the decline in native fish population.展开更多
Mortality is normal and natural in aquaculture experiments. However, it becomes a problem when measuring feed intake as a component of nutrient utilization parameters such as： feed conversion ratio, feed conversion e...Mortality is normal and natural in aquaculture experiments. However, it becomes a problem when measuring feed intake as a component of nutrient utilization parameters such as： feed conversion ratio, feed conversion efficiency, apparent net protein utilization, protein efficiency ratio and protein productive value. In order to accurately evaluate feed intake with due consideration of mortality encountered, a formulae was developed using empirical data obtained from an experiement that had mortality. The formulae Fwas： total feed consumed by numbers of survived fishes=DL∑i=1n（Fi/Ni）N. Where, D=Numbers of days fishes were fed within each period ibefore it was adjusted（e.g fortnight）, it was constant throught period of experiment; F=Uantity of feed fed per day for a fortnight before the quantity was adjusted, it changed every fortnight; L=Numbers of living fishes at the end of experimental period; N=Numbers of fishes at each weighing period, it might change every fortnight or might be constant if no mortality occurred; while n=Numbers of times fishes were weighed and quantity of feed was adjusted but this did not include the final weighing at the termination of experiment. However, if the fishes were weighed weekly, then D=6.展开更多
This study assessed the fish production, culture facilities, operations, water resource management and profitability of fish farming in Katsina State, Nigeria, with a view of understanding the status of aquaculture de...This study assessed the fish production, culture facilities, operations, water resource management and profitability of fish farming in Katsina State, Nigeria, with a view of understanding the status of aquaculture development in Katsina State. Data were collected using structured questionnaire administered to 35 out of the active 42 farms in Katsina State at the period and the data obtained were analyzed using descriptive statistics and linear regression. Among the respondents, 37.1% used concrete tank alone and another 37.2% used concrete tank with other types of culture facilities, 57.1% practised mono-culture techniques and 77.1% used stagnant renewal system as culture system. Management of water quality was done by majority(82.9%) with mere visual evaluation, while 68.6% did not use any forms of water treatment. Most of the farms(80%) depended on imported feed for feeding their fish and gross profits of ■7.29±1.81 and ■157.83±118.08 were obtained on fingerlings and adult fish, respectively. The tested explanatory variables were responsible for 45.4% change in profitability and profitability was found to be dependent on feeding cost(t=–3.38 and p=0.002) and size of fish at harvest(t=2.70 and p=0.011). The research findings established that fish farming in Katsina State was under developed.展开更多
In this paper, before the implementation of ecological laboratory experiments, the population interaction dynamics of an algae-fish system were studied mathematically and numerically. The purpose of this study was to ...In this paper, before the implementation of ecological laboratory experiments, the population interaction dynamics of an algae-fish system were studied mathematically and numerically. The purpose of this study was to explore how filter-feeding fish population affects the growth dynamics of the algae population. Mathematically theoretical works have been pursuing the investigation of some key conditions for stability of the equilibrium and existence of Hopf bifurcation. Numerical simulation works have been parsing the discovery of the growth dynamics of the algae population in view of population interaction dynamics, which in turn could prove the feasibility of the theoretical derivation and reveal the relationship between filter-feeding fish abundance and algal biomass in fish-drift algae communiyua. Furthermore, it was successful to show that the filter-feeding fish population may be a crucial factor in controlling the proliferation of the algae population, which could also directly grasp the evolution of community dynamics. All these results were expected to be useful in the study of community dynamics and laboratory elimination experiment of the algae population.展开更多
The Microcystis bloom that once disappeared from Donghu Lake, a shallow eutrophic Chinese lake,was successfully resumed with the use of the enclosure method. Seston and dissolved organic carbon (DOC)dynamics during th...The Microcystis bloom that once disappeared from Donghu Lake, a shallow eutrophic Chinese lake,was successfully resumed with the use of the enclosure method. Seston and dissolved organic carbon (DOC)dynamics during the Microcystis bloom were investigated with particular emphasis on the effect of three Chinese domestic fishes (silver carp, bighead carp and grass carp). The results showed that the change of chlorophyll a obviously reated to the fish species and its stocking density. Du to the relare of exracelular ogranic carbon and increased bacterial activity, DOC increased apparently after silver and bighead carps were introduced. POC/DOC dropped considerably in the enclosures containing silver and bighead carps and in the surrounding lake water, which implied that the bacterial acivity was more pronounced in such ecosystems. Statistical analytis indicated that the linear relationship between POC and chiorophyll a could reflect the state of algal growth and nutrient metabolism. It was expected that this study would provide some information on biomanipulation in the shallow eutrophic lakes.展开更多
由于传统淡水鱼池塘养殖存在废物过量堆积、水体污染严重等问题,以及中国对水产品需求的增加,工厂化循环水养殖、集装箱、圈养模式等高质量、高产量、集约化的新型淡水鱼养殖模式逐渐得到应用。针对新型淡水鱼养殖模式存在投饲劳动强度...由于传统淡水鱼池塘养殖存在废物过量堆积、水体污染严重等问题,以及中国对水产品需求的增加,工厂化循环水养殖、集装箱、圈养模式等高质量、高产量、集约化的新型淡水鱼养殖模式逐渐得到应用。针对新型淡水鱼养殖模式存在投饲劳动强度大、自动化程度低等问题,设计了一款气送式自动投饲系统,实现了定向、定时和定量投饲功能。设计了特定的四通装置和三通装置以保证饲料的定向输送;基于计算流体力学和离散单元法(Computational fluid dynamics-Discrete element method,CFD-DEM)耦合技术,初步确定了满足投饲要求的投饲速度并由此确定了风机型号;开发了以Arduino Mega 2560单片机为核心处理器的投饲控制系统,通过时钟模块实时获取当前时间信息,通过称量传感器实时获取料仓内物料质量信息,通过蓝牙传输预计投饲区域、投饲时间和投饲质量,从而实现定向定时定量投饲;最后试制样机并进行了性能试验,两种工况下投饲的实际投饲距离与仿真结果误差分别为5.74%和9.54%,定向、定时和定量投饲可以准确实现,控制系统响应时间小于1 s,定量投饲最大误差为5.37%。研究结果表明,自动投饲系统结构可靠、控制系统精度较高,满足自动投饲要求。展开更多
文摘Aflatoxin contamination and rancidity in locally processed commercial fish feeds and ingredients along value chains is a Public and Animal health hazard. The study established the level of aflatoxin contamination, peroxide value (PV), Anisidine value (AnV), and their associated factors at storage areas among farmers, processors, traders, factories, and landing sites in the Wakiso and Kampala districts. The value chain actors were purposively selected in a cross-sectional study based on access to the feed store and the use of locally processed commercial fish feeds on farms. Data collected were statistically analyzed in SPSS version 20. All the samples (45) were positive for aflatoxin contamination and PV with 51% (23/45) of samples being contaminated with “above acceptable” aflatoxin levels and 66.6% (30/45) of samples with “above acceptable” PV. The overall percentage of “above acceptable” AnV was 11/29 (37.9%). Samples from factories were within acceptable contamination levels. Multivariate logistic regression analysis revealed no significant difference between aflatoxin contamination, peroxide, and Anisidine value with storage factors for locally processed commercial fish feeds and ingredients. The study recommended the purchase of fish feeds from factories and a larger study on storage factors responsible for aflatoxin contamination and rancidity in fish feeds in Uganda.
文摘PBDEs are widely used brominated flame retardant, which are increasingly reported in the environment. Concentrations of thirteen PBDEs in a large number of channel catfish and fish feeds collected from Hubei province of China were investigated in this study. A fast isotopic dilution GC-MS method was firstly developed to simultaneously determine thirteen PBDEs in channel catfish and fish feeds in this study, and especially for the first time PBDEs in fish feeds. Pressurized liquid extraction and multi-layer silica gel column chromatography cleanup were used, some important steps and crucial parameters were modified and intensified compared with other literatures, and GC and MS conditions were optimized. The limits of quantitation values of 0.25 - 5 μg·kg-1 wet weight in channel catfish were calculated for PBDEs;so did 1 - 20 μg·kg-1 wet weight in fish feeds. In addition, good repeatability and accuracy of the whole method were achieved. The established methods were therefore suitable for the simultaneous determinations of thirteen PBDEs in these samples at trace contamination levels. Using the established methods, PBDEs emerged in 10 of 180 channel catfish and 13 of 115 fish feeds, and the dominant PBDEs homologues were PBDE-28, PBDE-47, PBDE-100, PBDE-99, PBDE-154, PBDE-153 and PBDE-183. The detection rates of seven PBDEs congeners were in turn respectively 1.74%, 7.83%, 4.35%, 5.22%, 3.48%, 2.61% and 2.61% in fish feeds, and detection rates of seven PBDEs congeners were in turn respectively 1.11%, 3.89%, 2.22%, 1.67%, 1.11%, 1.11% and 1.11% in channel catfish. There was significant correlation in PBDEs between matching channel catfish and fish feeds (R2 = 0.742, P < 0.001, n = 30), suggesting that fish feeds contaminated with PBDEs possibly resulted in PBDEs residual in channel catfish through biological transfer.
基金Supported by the National Key Technology R&D Program(No.2015BAD13B06)the Finance Special Fund of Chinese Ministry of Agriculture(Fisheries Resources and Environment Survey in the Key Water Areas of Tibet)
文摘In recent years, fish invasion has become one of the main reasons for the decline of native fish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated the fish resources of the Chabalang Wetland (Lhasa, Tibet) during diff erent seasons in 2009 and 2013. Four hundred and twelve individuals were subsampled to estimate age, growth, and feeding habit of P . parva . Furthermore, food relationships between P . parva and the native Schizothoracinae fish were also examined. The results revealed a significant shift in species composition and community structure characterized by the disappearance of native fish and outbreak of non-native fish. The percentage of nonnative P . parva in the fish collections significantly increased from 33.64% in 2009 to 64.08% in 2013. The standard length (SL) ranged from 22.00 to 78.71 mm, and their age was 1–5 a. The von Bertalanff y function was used to model the observed length-at-age data as L t =112.19(1–e^-0.1495 ( t +0.8012)) for females and as L t =123.12 (1–e^-0.1500 ( t +0.7132)) for males. The results indicated that P . parva in Tibet has lower growth and mortality rates compared with that from the native ranges. Ninety-seven prey taxa belonging to 9 prey categories were identified in the gut of 38 P . parva . P . parva can be considered a generalized and opportunistic predator, competing with the native fish, especially Schizothorax o’connori , Schizopygopsis younghusbandi younghusbandi , and Ptychobarbus dipogon , for Bacillariophyta and Chironomid larvae. This is an important reason for the decline in native fish population.
文摘Mortality is normal and natural in aquaculture experiments. However, it becomes a problem when measuring feed intake as a component of nutrient utilization parameters such as： feed conversion ratio, feed conversion efficiency, apparent net protein utilization, protein efficiency ratio and protein productive value. In order to accurately evaluate feed intake with due consideration of mortality encountered, a formulae was developed using empirical data obtained from an experiement that had mortality. The formulae Fwas： total feed consumed by numbers of survived fishes=DL∑i=1n（Fi/Ni）N. Where, D=Numbers of days fishes were fed within each period ibefore it was adjusted（e.g fortnight）, it was constant throught period of experiment; F=Uantity of feed fed per day for a fortnight before the quantity was adjusted, it changed every fortnight; L=Numbers of living fishes at the end of experimental period; N=Numbers of fishes at each weighing period, it might change every fortnight or might be constant if no mortality occurred; while n=Numbers of times fishes were weighed and quantity of feed was adjusted but this did not include the final weighing at the termination of experiment. However, if the fishes were weighed weekly, then D=6.
文摘This study assessed the fish production, culture facilities, operations, water resource management and profitability of fish farming in Katsina State, Nigeria, with a view of understanding the status of aquaculture development in Katsina State. Data were collected using structured questionnaire administered to 35 out of the active 42 farms in Katsina State at the period and the data obtained were analyzed using descriptive statistics and linear regression. Among the respondents, 37.1% used concrete tank alone and another 37.2% used concrete tank with other types of culture facilities, 57.1% practised mono-culture techniques and 77.1% used stagnant renewal system as culture system. Management of water quality was done by majority(82.9%) with mere visual evaluation, while 68.6% did not use any forms of water treatment. Most of the farms(80%) depended on imported feed for feeding their fish and gross profits of ■7.29±1.81 and ■157.83±118.08 were obtained on fingerlings and adult fish, respectively. The tested explanatory variables were responsible for 45.4% change in profitability and profitability was found to be dependent on feeding cost(t=–3.38 and p=0.002) and size of fish at harvest(t=2.70 and p=0.011). The research findings established that fish farming in Katsina State was under developed.
文摘In this paper, before the implementation of ecological laboratory experiments, the population interaction dynamics of an algae-fish system were studied mathematically and numerically. The purpose of this study was to explore how filter-feeding fish population affects the growth dynamics of the algae population. Mathematically theoretical works have been pursuing the investigation of some key conditions for stability of the equilibrium and existence of Hopf bifurcation. Numerical simulation works have been parsing the discovery of the growth dynamics of the algae population in view of population interaction dynamics, which in turn could prove the feasibility of the theoretical derivation and reveal the relationship between filter-feeding fish abundance and algal biomass in fish-drift algae communiyua. Furthermore, it was successful to show that the filter-feeding fish population may be a crucial factor in controlling the proliferation of the algae population, which could also directly grasp the evolution of community dynamics. All these results were expected to be useful in the study of community dynamics and laboratory elimination experiment of the algae population.
文摘The Microcystis bloom that once disappeared from Donghu Lake, a shallow eutrophic Chinese lake,was successfully resumed with the use of the enclosure method. Seston and dissolved organic carbon (DOC)dynamics during the Microcystis bloom were investigated with particular emphasis on the effect of three Chinese domestic fishes (silver carp, bighead carp and grass carp). The results showed that the change of chlorophyll a obviously reated to the fish species and its stocking density. Du to the relare of exracelular ogranic carbon and increased bacterial activity, DOC increased apparently after silver and bighead carps were introduced. POC/DOC dropped considerably in the enclosures containing silver and bighead carps and in the surrounding lake water, which implied that the bacterial acivity was more pronounced in such ecosystems. Statistical analytis indicated that the linear relationship between POC and chiorophyll a could reflect the state of algal growth and nutrient metabolism. It was expected that this study would provide some information on biomanipulation in the shallow eutrophic lakes.
文摘由于传统淡水鱼池塘养殖存在废物过量堆积、水体污染严重等问题,以及中国对水产品需求的增加,工厂化循环水养殖、集装箱、圈养模式等高质量、高产量、集约化的新型淡水鱼养殖模式逐渐得到应用。针对新型淡水鱼养殖模式存在投饲劳动强度大、自动化程度低等问题,设计了一款气送式自动投饲系统,实现了定向、定时和定量投饲功能。设计了特定的四通装置和三通装置以保证饲料的定向输送;基于计算流体力学和离散单元法(Computational fluid dynamics-Discrete element method,CFD-DEM)耦合技术,初步确定了满足投饲要求的投饲速度并由此确定了风机型号;开发了以Arduino Mega 2560单片机为核心处理器的投饲控制系统,通过时钟模块实时获取当前时间信息,通过称量传感器实时获取料仓内物料质量信息,通过蓝牙传输预计投饲区域、投饲时间和投饲质量,从而实现定向定时定量投饲;最后试制样机并进行了性能试验,两种工况下投饲的实际投饲距离与仿真结果误差分别为5.74%和9.54%,定向、定时和定量投饲可以准确实现,控制系统响应时间小于1 s,定量投饲最大误差为5.37%。研究结果表明,自动投饲系统结构可靠、控制系统精度较高,满足自动投饲要求。
