Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, d...Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denatura-tion, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.展开更多
The Interaction between vitamin B12 （VBI2） and fish sperm DNA was investigated in physiological buffer （pH 7.4） using the methylene blue （MB） dye as a spectral probe by spetcrophotometery, viscosity measurements...The Interaction between vitamin B12 （VBI2） and fish sperm DNA was investigated in physiological buffer （pH 7.4） using the methylene blue （MB） dye as a spectral probe by spetcrophotometery, viscosity measurements and cyclic voltammetry. The apparent binding constant of vitamin B12 with DNA was found to be 3.2 × 10^5 mol^-1·L. The voltammetric behavior of vitamin B12 has been investigated at glassy carbon electrode using cyclic voltammetry. Thermodynamic parameters including △H^0, △S^0 and △G^0 for the interaction between VB12 and DNA have determined as -2.3 × 10^4, 27.54 and -3.1 × 10^4j·mol^-l·K^-1 respectively. One indication of DNA binding mode with VBI2 was the change in viscosity when a small molecule associates with DNA. The diffusion coefficients of VB12 in the absence （D0）f and presence of DNA （D0）b was calculated as 5.04 × 10^-6 and 1.13 × 10^-6 cm^2·s^-1 respectively. The results indicated that vitamin B12 can bind to DNA and the major binding mode was intercalative binding.展开更多
基金Funded by the National High Technology R&D Program of China (863 Program, Nos. 2001AA621100, 2003AA603510 and 2004AA603310).
文摘Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denatura-tion, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.
文摘The Interaction between vitamin B12 （VBI2） and fish sperm DNA was investigated in physiological buffer （pH 7.4） using the methylene blue （MB） dye as a spectral probe by spetcrophotometery, viscosity measurements and cyclic voltammetry. The apparent binding constant of vitamin B12 with DNA was found to be 3.2 × 10^5 mol^-1·L. The voltammetric behavior of vitamin B12 has been investigated at glassy carbon electrode using cyclic voltammetry. Thermodynamic parameters including △H^0, △S^0 and △G^0 for the interaction between VB12 and DNA have determined as -2.3 × 10^4, 27.54 and -3.1 × 10^4j·mol^-l·K^-1 respectively. One indication of DNA binding mode with VBI2 was the change in viscosity when a small molecule associates with DNA. The diffusion coefficients of VB12 in the absence （D0）f and presence of DNA （D0）b was calculated as 5.04 × 10^-6 and 1.13 × 10^-6 cm^2·s^-1 respectively. The results indicated that vitamin B12 can bind to DNA and the major binding mode was intercalative binding.
