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水貂铜绿假单胞菌鞭毛分型及分离株flic基因的克隆与序列分析 被引量:2
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作者 杨培培 王颖 +7 位作者 胡继明 杨瑞梅 张传美 韩先杰 黄娟 孙月平 秦晓冰 单虎 《中国预防兽医学报》 CAS CSCD 北大核心 2012年第9期749-751,共3页
为分析铜绿假单胞菌(PA)分离株鞭毛蛋白基因(flic)之间的差异,本研究通过PCR方法扩增由山东地区发病水貂的肺脏组织中分离的29株分离株flic基因的部分片段,根据其片段长度确定PA的鞭毛类型。选取两种类型代表株(PASD01、PASD03),采用PC... 为分析铜绿假单胞菌(PA)分离株鞭毛蛋白基因(flic)之间的差异,本研究通过PCR方法扩增由山东地区发病水貂的肺脏组织中分离的29株分离株flic基因的部分片段,根据其片段长度确定PA的鞭毛类型。选取两种类型代表株(PASD01、PASD03),采用PCR方法扩增flic全基因进行序列分析。结果表明,PASD01分离株与国外A型PA flic基因序列同源性为99.6%~99.7%,PASD03分离株与B型PA flic基因的序列同源性为98.9%~99.5%,而两个分离株之间的序列同源性为76%。本研究首次从水貂病变组织中克隆得到两种鞭毛蛋白类型的flic基因,该基因在各型鞭毛菌株中高度保守,为进一步研制鞭毛亚单位疫苗提供实验依据。 展开更多
关键词 水貂铜绿假单胞菌 鞭毛分型 flic基因 克隆 序列分析
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Collective motion of bacteria in two dimensions
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作者 Yilin Wu 《Frontiers of Electrical and Electronic Engineering in China》 CSCD 2015年第4期199-205,共7页
Collective motion can be observed in biological systems over a wide range of length scales, from large animals to bacteria. Collective motion is thought to confer an advantage for defense and adaptation. A central que... Collective motion can be observed in biological systems over a wide range of length scales, from large animals to bacteria. Collective motion is thought to confer an advantage for defense and adaptation. A central question in the study of biological collective motion is how the traits of individuals give rise to the emergent behavior at population level This question is relevant to the dynamics of general self-propelled particle systems, biological self-organization, and active fluids. Bacteria provide a tractable system to address this question, because bacteria are simple and their behavior is relatively easy to control. In this mini review we will focus on a special form of bacterial collective motion, i.e., bacterial swarming in two dimensions. We will introduce some organization principles known in bacterial swarming and discuss potential means of controlling its dynamics. The simplicity and controllability of 2D bacterial behavior during swarming would allow experimental examination of theory predictions on general collective motion. 展开更多
关键词 bacterial swarming BIOFILM flageuar motility gliding motility biological self-organization emergentbehavior
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