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Fine mapping of two recessive genes TaFLA1 and TaSPL8 controlling flag leaf angle in bread wheat
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作者 Qiushi Wang Jiaxing Bai +11 位作者 Hongchun Xiong Yongdun Xie Chaojie Wang Jiayu Gu Linshu Zhao Huiyuan Li Jinfeng Zhang Shirong Zhao Yuping Ding Zhengwu Fang Huijun Guo Luxiang Liu 《The Crop Journal》 SCIE CSCD 2024年第4期1159-1167,共9页
Flag leaf angle is one of the key target traits in high yield wheat breeding.A smaller flag leaf angle reduces shading and enables plants to grow at a higher density,which increases yield.Here we identified a mutant,j... Flag leaf angle is one of the key target traits in high yield wheat breeding.A smaller flag leaf angle reduces shading and enables plants to grow at a higher density,which increases yield.Here we identified a mutant,je0407,with an 84.34%-89.35%smaller flag leaf angle compared with the wild type.The mutant also had an abnormal lamina joint and no ligule or auricle.Genetic analysis indicated that the ligule was controlled by two recessive genes,which were mapped to chromosomes 2AS and 2DL.The mutant allele on chromosome 2AS was named Tafla1b,and it was fine mapped to a 1 Mb physical interval.The mutant allele on chr.2DL was identified as Taspl8b,a novel allele of TaSPL8 with a missense mutation in the second exon,which was used to develop a cleaved amplified polymorphic sequence marker.F3 and F4 lines derived from crosses between Jing411 and je0407 were genotyped to investigate interactions between the Tafla1b and Taspl8b alleles.Plants with the Tafla1b/Taspl8a genotype had 58.41%-82.76%smaller flag leaf angles,6.4%-24.9%shorter spikes,and a greater spikelet density(0.382 more spikelets per cm)compared with the wild type.Plants with the Tafla1a/Taspl8b genotype had 52.62%-82.24%smaller flag leaf angles and no differences in plant height or spikelet density compared with the wild type.Tafla1b/Taspl8b plants produced erect leaves with an abnormal lamina joint.The two alleles had dosage effects on ligule formation and flag leaf angle,but no significant effect on thousand-grain weight.The mutant alleles provide novel resources for improvement of wheat plant architecture. 展开更多
关键词 WHEAT Ligule Flag leaf angle Fine mapping Tafla1 Taspl8
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The Reading Movement in Shanghai,China in the Late 1950s
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作者 LIU Hao JING Huilan 《International Relations and Diplomacy》 2024年第2期86-90,共5页
At the end of the 1950s,Shanghai region of China carried out the Lu Xun Medal Reading Campaign and the Red Flag Reading Campaign one after another,aiming to guide young people to read good books and cultivate a positi... At the end of the 1950s,Shanghai region of China carried out the Lu Xun Medal Reading Campaign and the Red Flag Reading Campaign one after another,aiming to guide young people to read good books and cultivate a positive interest in life through reading,which is a gentle and constant form of ideological education.Based on the historical materials collected by Shanghai Archives,the author investigated the background,development,main links,and effects of the reading movement.The research shows that the reading movement has promoted young peoples political awareness,broadened their knowledge horizons,and promoted the formation of a good social atmosphere. 展开更多
关键词 Lu Xun Medal Reading Campaign Red Flag Reading Campaign YOUTH ideological education
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DIRECT PROOF OF THE DETERMINANT EXPRESSION FOR FLAGGED SKEW TABLEAUX
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作者 初文昌 《Acta Mathematicae Applicatae Sinica》 SCIE CSCD 1992年第4期318-322,共5页
Flagged skew tableaux are generalizationa of Young tableaux in which each row (column)has an upper and lower bound on the entries. It has been shown that they are enumerated byflagged skew Schur functions. By introduc... Flagged skew tableaux are generalizationa of Young tableaux in which each row (column)has an upper and lower bound on the entries. It has been shown that they are enumerated byflagged skew Schur functions. By introducing the dominance technique, we preeent an alternateproof for this conclusion directly. 展开更多
关键词 DIRECT PROOF OF THE DETERMINANT EXPRESSION FOR flagged SKEW TABLEAUX
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FLAG标签纳米抗体的筛选、表达及验证
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作者 王欣怡 王晓倩 +1 位作者 王红军 晁跃辉 《生物技术通报》 CAS CSCD 北大核心 2023年第10期323-331,共9页
纳米抗体是一种新型的蛋白质工程抗体,其体积小、稳定性强、亲和力高等特性为科学研究提供了新的可能性。FLAG标签是一种广泛应用于生物学研究中的短肽标签,在生物学研究中具有重要的作用。为了制备FLAG标签的纳米抗体,利用酵母双杂交... 纳米抗体是一种新型的蛋白质工程抗体,其体积小、稳定性强、亲和力高等特性为科学研究提供了新的可能性。FLAG标签是一种广泛应用于生物学研究中的短肽标签,在生物学研究中具有重要的作用。为了制备FLAG标签的纳米抗体,利用酵母双杂交技术筛选出具有高亲和力的纳米抗体,并对制备的FLAG纳米抗体进行性能检验。通过DNA重组技术,构建包含FLAG的诱饵载体,利用酵母双杂交技术,在驼源纳米抗体酵母文库中,筛选针对FLAG标签纳米抗体。在酵母文库中筛选出5个单一的候选抗体DNA序列,为了排除载体本身表达蛋白序列对抗体筛选带来的干扰,通过“点对点”验证方法排除非特异性杂交的可能,通过该操作确认所筛选的5株纳米抗体均能与FLAG标签发生特异性亲和反应。为了制备纳米抗体,构建了5个纳米抗体原核表达载体,并利用大肠杆菌体系进行表达。通过SDS-PAGE和Western杂交(WB)分析,结果显示,成功获得2株可溶性表达的抗FLAG标签蛋白纳米抗体。对这2株纳米抗体与商品化的常规FLAG标签抗体进行效果比对,结果显示制备的2株纳米抗体与商品化抗体均能够识别FLAG多肽及含有FLAG标签的融合蛋白,且在特异性上没有明显区别,表明制备的FLAG纳米抗体具有较好的应用前景。基于酵母双杂交技术,成功筛选并制备了FLAG标签纳米抗体,这一成果不仅丰富了纳米抗体的类型,也为抗体开发及应用提供了新的途径。该研究为进一步研究FLAG标签在生物学研究中的应用,以及纳米抗体在生物工程中的应用提供有力支持。 展开更多
关键词 FLAG标签 纳米抗体 酵母双杂 筛选 表达 特异性 免疫沉淀
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绵羊PLC-γ1基因真核表达载体的构建及鉴定
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作者 袁利明 陈云蕾 +3 位作者 刘素平 丁林玲 吴兰 赛务加甫 《石河子大学学报(自然科学版)》 CAS 北大核心 2023年第4期441-448,共8页
目的旨在构建携带红色荧光蛋白(RFP)报告基因的绵羊PLC-γ1非融合性真核表达载体。方法在原有PUC57-PLC-γ1克隆载体基础上,对目的片段N端前加入P2A序列和3个连续Flag序列后使用HindⅢ酶和SalⅠ酶双酶切获得P2A-3×Flag-PLC-γ1片段... 目的旨在构建携带红色荧光蛋白(RFP)报告基因的绵羊PLC-γ1非融合性真核表达载体。方法在原有PUC57-PLC-γ1克隆载体基础上,对目的片段N端前加入P2A序列和3个连续Flag序列后使用HindⅢ酶和SalⅠ酶双酶切获得P2A-3×Flag-PLC-γ1片段,将其连接至pDsRed2-C1载体获得重组质粒pDsRed2-C1-P2A-3×Flag-PLC-γ1;然后采用Lip 2000转染试剂将该重组质粒转染至HEK-293T细胞,最后利用荧光显微镜、qRT-PCR、Western blot、免疫共沉淀(IP)和免疫荧光(IF)法鉴定重组质粒pDsRed2-C1-P2A-3×Flag-PLC-γ1的表达及亚细胞定位情况。结果双酶切和测序结果显示质粒pDsRed2-C1-P2A-3×Flag-PLC-γ1构建成功,观察到红色荧光;免疫荧光的亚细胞定位检测出PLC-γ1蛋白在细胞质中表达;qRT-PCR和Western blotting法均能检测出PLC-γ1表达,与对照组有显著性差异(P>0.01);免疫共沉淀也纯化分离出含有Flag标签的PLC-γ1蛋白。结论本研究成功构建绵羊PLC-γ1基因的真核表达载体,在P2A肽作用下实现目的蛋白PLC-γ1与DsRed2的表达,构建的载体可用于研究PLC-γ1在早期胚胎发育中的作用。 展开更多
关键词 P2A肽 PLC-γ1 绵羊 HEK-293T细胞 Flag标签
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C端带Flag标签重组小鼠IL-33慢病毒载体的构建及鉴定
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作者 高二可 张路 +1 位作者 徐旺旺 金齐力 《牡丹江医学院学报》 2023年第3期18-20,26,共4页
目的构建C端携带Flag标签蛋白的重组小鼠IL-33慢病毒表达载体,为进一步研究IL-33在肿瘤免疫中的作用提供实验基础。方法设计引物在小鼠IL-33基因的C端加入Flag标签,PCR法扩增带有标签的目的基因,再接入经酶切后的线性化pLVX-puro慢病毒... 目的构建C端携带Flag标签蛋白的重组小鼠IL-33慢病毒表达载体,为进一步研究IL-33在肿瘤免疫中的作用提供实验基础。方法设计引物在小鼠IL-33基因的C端加入Flag标签,PCR法扩增带有标签的目的基因,再接入经酶切后的线性化pLVX-puro慢病毒载体。带标签的目的基因重组载体用两种限制性内切酶ApaI和XhoI酶切后,进行琼脂糖凝胶电泳鉴定,最后测定目的基因序列。结果成功构建重组小鼠IL-33慢病毒表达载体并将Flag标签成功插入到目的基因的C端。结论成功构建了重组慢病毒表达载体pLVX-mIL-33-Flag,为下一步在肿瘤中研究IL-33的作用和机制奠定了基础。 展开更多
关键词 载体构建 Flag标签 IL-33 肿瘤免疫
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RANDERS SPACES WITH SCALAR FLAG CURVATURE
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作者 李锦堂 《Acta Mathematica Scientia》 SCIE CSCD 2023年第3期994-1006,共13页
Let(M, F) be an n-dimensional Randers space with scalar flag curvature. In this paper, we will introduce the definition of a weak Einstein manifold. We can prove that if(M, F) is a weak Einstein manifold, then the fla... Let(M, F) be an n-dimensional Randers space with scalar flag curvature. In this paper, we will introduce the definition of a weak Einstein manifold. We can prove that if(M, F) is a weak Einstein manifold, then the flag curvature is constant. 展开更多
关键词 Randers spaces flag curvature sectional curvature weak Einstein manifold
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Genetic effects of Agropyron cristatum 2P chromosome translocation fragments in a wheat background
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作者 XU Shi-rui JIANG Bo +7 位作者 HAN Hai-ming JI Xia-jie ZHANG Jin-peng ZHOU Sheng-hui YANG Xin-ming LI Xiu-quan LI Li-hui LIU Wei-hua 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第1期52-62,共11页
Agropyron cristatum(2n=4x=28,PPPP)is a wild relative of common wheat which contains a large number of desirable genes that can be exploited for wheat improvement.Wheat–A.cristatum 2P alien translocation lines exhibit... Agropyron cristatum(2n=4x=28,PPPP)is a wild relative of common wheat which contains a large number of desirable genes that can be exploited for wheat improvement.Wheat–A.cristatum 2P alien translocation lines exhibit many desirable traits,such as small flag leaves,a high spikelet number and density,and a compact plant type.An agronomic trait evaluation and a genetic analysis were carried out on translocation lines and backcross populations of these lines carrying different translocation fragments.The results showed that a translocation fragment from 2PT-3(2PL)reduced the length of the flag leaves,while translocation fragments from 2PT-3(2PL)and 2PT-5(2PL(0.60–1.00))reduced the width of the flag leaves.A translocation fragment from 2PT-13(2PS(0.18–0.36))increased the length and area of the flag leaves.Translocation fragments from 2PT-3(2PL)and 2PT-8(2PL(0.86–1.00))increased the density of spikelets.Translocation fragments from 2PT-7(2PL(0.00–0.09)),2PT-8(2PL(0.86–1.00)),2PT-10(2PS),and 2PT-13(2PS(0.18–0.36))reduced plant height.This study provides a scientific basis for the effective utilization of wheat–A.cristatum translocation lines. 展开更多
关键词 wheat-A.cristatum 2P chromosome translocation lines flag leaf spikelet density genetic effects
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东芝进一步扩展Thermoflagger产品线
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《单片机与嵌入式系统应用》 2023年第11期95-95,共1页
东芝电子元件及存储装置株式会社(东芝)宣布,进一步扩展Thermoflagger过温检测IC产品线---“TCTH0xxxE系列”。该系列可用于具有正温度系数(PTC)热敏电阻的简单电路中,用来检测电子设备中的温度升高,六款新产品于今日开始支持批量出货... 东芝电子元件及存储装置株式会社(东芝)宣布,进一步扩展Thermoflagger过温检测IC产品线---“TCTH0xxxE系列”。该系列可用于具有正温度系数(PTC)热敏电阻的简单电路中,用来检测电子设备中的温度升高,六款新产品于今日开始支持批量出货。当配置在具有PTC热敏电阻(其电阻值随温度变化而变化)的简单电路中时,Thermoflagger过温检测IC不仅可以检测温度升高,还可以检测置于热源附近的PTC热敏电阻的电阻变化,并在温度过高时输出FLAG信号。例如,当Thermoflagger检测到异常发热情况并向MCU发送FLAG信号时,MCU将关闭设备或改变正在产热的设备或半导体的运行方式。通过串联PTC热敏电阻可同时在多个点进行过温检测。 展开更多
关键词 PTC热敏电阻 简单电路 存储装置 电阻变化 FLAG 电子设备 电子元件
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Screening for Transthyretin Cardiac Amyloidosis in Patients with Bilateral Carpal Tunnel Syndrome: Identifying Missed Opportunities for Early Detection and Treatment
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作者 Ayman Alsaadi Renato Cerna-Viacava +6 位作者 Liyan Obeidat Shing Chao Johnathan Stephan Charles S. Day Jennifer Cowger Celeste Williams Karthikeyan Ananthasubramaniam 《World Journal of Cardiovascular Diseases》 2023年第7期366-376,共11页
Purpose: Transthyretin cardiac amyloidosis (ATTR-CA) has been linked to many extra-cardiac manifestations including bilateral carpal tunnel syndrome (CTS). The aim of this study is to analyze patients with bilate... Purpose: Transthyretin cardiac amyloidosis (ATTR-CA) has been linked to many extra-cardiac manifestations including bilateral carpal tunnel syndrome (CTS). The aim of this study is to analyze patients with bilateral CTS to identify patients with high-risk features or “red flags” for ATTR-CA, identify if systematic screening was done for ATTR-CA and define opportunities for improved detection. Methods: Out of >5000 patients with bilateral CTS evaluated in a single tertiary care center in Southeast Michigan (2010-2016), we retrospectively studied a focused population of patients: men > 50 years and women > 60 years old with bilateral CTS and atrial fibrillation (n = 295). Baseline demographic, comorbidities, and electrocardiographic and echocardiographic findings were analyzed. A high-risk group suspicious for ATTR-CA was identified as patients with bilateral CTS, atrial fibrillation, and concomitant “red flags” including heart failure and left ventricular hypertrophy. Results: Out of 295 patients, 51.2% were female, 75.6% were White, and 22.4% were African American. Upon comparing the high-risk group (n = 67) with the remaining study population (n = 228), both diagnosis of ATTR-CA and mortality were higher among the high-risk group (7.5% vs 0.4% and 43.3% vs 24.6%, respectively, P = 0.003). Conclusions: A substantial number of bilateral CTS patients had additional “red flags” warranting formal evaluation for ATTR-CA;however, systematic evaluation for cardiac amyloidosis was not performed in many patients. This emphasizes that Multidisciplinary collaboration is needed to create a systematic workflow and to raise awareness amongst cardiologists and other physicians for suspecting ATTR-CA in bilateral CTS patients who have additional “red flags”. 展开更多
关键词 Bilateral Carpal Tunnel Syndrome MORALITY PREVALENCE Red Flags Transthyretin Cardiac Amyloidosis
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一种具有flag板的自动化轨道吊大车定位装置及其应用探究
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作者 李文 《珠江水运》 2023年第20期56-58,共3页
目前自动化轨道吊堆场在使用期间会出现沉降或者凸起现象,轨道吊在大车行进期间会造成大车对射开关无法感应到flag板以及大车对射开关支架与flag板碰撞损坏的情况,通过安装可调式的flag板,可在堆场实际沉降或者凸起的地方对flag板进行... 目前自动化轨道吊堆场在使用期间会出现沉降或者凸起现象,轨道吊在大车行进期间会造成大车对射开关无法感应到flag板以及大车对射开关支架与flag板碰撞损坏的情况,通过安装可调式的flag板,可在堆场实际沉降或者凸起的地方对flag板进行高度的改变,以满足自动化轨道吊大车行走机构精准定位以及行进时实时位置校验,提高自动化轨道吊的作业效率。 展开更多
关键词 港口 轨道吊 flag板 大车精准定位系统
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From People as Foundation(Minbon)to Concept of a Republic(Minkuk):Tracing Neo-Confucianism in Korean History
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作者 Yi Tae-jin 《Cultural and Religious Studies》 2023年第3期105-117,共13页
This article is the memoirs of the author’s long-time research on the Neo-Confucian history of the Chosun dynasty Korea.The author recalled his academic achievement of the several stages of the historical changes of ... This article is the memoirs of the author’s long-time research on the Neo-Confucian history of the Chosun dynasty Korea.The author recalled his academic achievement of the several stages of the historical changes of Korean society with the Neo-Confucianism.The time range covered from the late 14th century to the nineteenth century.The research position was adhered to the view of the positive function of Confucianism which aimed to overcome the negative understanding highly influenced by the Japanese colonialism.He examined the socio-economic development accompanied with the Neo-Confucianism at the first stage.In the part of the sixteenth century the formation of Sarim group was interpreted as critical forces and the prevailed factionalism as the principle of politics rather than the chronic political strife.The author introduced his unique achievement that the ideology of the Neo-Confucianism had been strongly intensified with the response to the long term natural catastrophes of the Little Ice Age(c.1490-1760).The republican concept of 18th-century impartial rulers,the most important achievement of Korean Neo-Confucianism,was viewed as the result of effort to overcome the natural disasters.This topic was connected to the concept of republic which shows in a diagram of the Korean national flag. 展开更多
关键词 Chiangnan Agriculture Long Term Natural catastrophes Concept of Republic Korean National Flag
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改良FLAG方案治疗33例难治复发性急性白血病的初步分析 被引量:15
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作者 孟凡义 杨龙江 +6 位作者 徐兵 刘晓力 郑维扬 张钰 黄芬 孙竞 刘启发 《癌症》 SCIE CAS CSCD 北大核心 2003年第12期1330-1333,共4页
背景与目的:FLAG方案用于治疗难治复发性急性非淋巴细胞性白血病(acutenon-lymphocyticleukemia,ANLL)已有多年,大多报道的CR率为50%~64%。本研究探讨改良FLAG方案(减少合并应用Ara-C剂量并在化疗前不用G-CSF)能否达到同样疗效,并减轻... 背景与目的:FLAG方案用于治疗难治复发性急性非淋巴细胞性白血病(acutenon-lymphocyticleukemia,ANLL)已有多年,大多报道的CR率为50%~64%。本研究探讨改良FLAG方案(减少合并应用Ara-C剂量并在化疗前不用G-CSF)能否达到同样疗效,并减轻不良反应。方法:33例成人急性白血病中难治性ANLL16例,难治性急性淋巴细胞白血病(ALL)12例,复发性ALL5例。全部病例接受氟达拉宾30mg·(m2·d)-1,静滴,第1~5天;其中合并Ara-C200mg·d-1有18例,Ara-C500mg·d-1有5例,Ara-C1000mg·d-1有10例,全部静脉滴注5~7天为1疗程。应用Ara-C200mg·d-1组和ALL组化疗前不用G-CSF,ALL患者每周加用长春新碱2mg,共2次;强的松60~80mg·d-1,共14天。化疗后WBC<1.0×109/L者加用G-CSF,剂量均为300μg·d-1,皮下注射至WBC3.0×109/L以上。每疗程完成后复查骨髓。结果:16例难治性ANLL的CR率为56.3%,而12例难治性ALL的CR率为8.3%(P<0.01);难治性ANLL患者中Ara-C200mg·d-1组的CR率高于500~1000mg·d-1组(70%∶33%),但无统计学差异(P>0.05)。化疗后WBC0.6×109/L和血小板15.6×109/L的平均持续时间分别为5天和4.3天,Ara-C200mg·d-1组感染发生率明显低于500~1000mg·d-1Ara-C组(58.0%∶85.7%)(P<0.05)。结论:与经典的FLAG方案相比。 展开更多
关键词 改良FLAG方案 治疗 难治复发性急性白血病 氟达拉宾 阿糖胞苷
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Studies on the Characteristics of Chlorophyll Fluorescence of Winter Wheat Flag Leaves at Different Developing Stages 被引量:28
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作者 卢庆陶 李卫华 +4 位作者 蒋高明 戈巧英 郝乃斌 孙家柱 郭仁峻 《Acta Botanica Sinica》 CSCD 2001年第8期801-804,共4页
The parameters of fluorescence induction kinetics and the maximal light-saturated net CO2 assimilation rate (P-sat) of the flag leaves of four cultivars of winter wheat (Triticum aestivum L.) were compared at three di... The parameters of fluorescence induction kinetics and the maximal light-saturated net CO2 assimilation rate (P-sat) of the flag leaves of four cultivars of winter wheat (Triticum aestivum L.) were compared at three different developing stages for the first time. From the blooming stage to the milky stage, the quantum efficiency of PS II photochemistry (F-v/F-m) declined slightly only at the milk stage. The photochemical quenching co-efficient (qP), actual quantum yield of photosystem II (PS II)electron transport (Phi (PSII)) and P-sat decreased substantially (> 15%), while the non-photochemical quenching co-efficient (qN) increased significantly (> 100%). There existed a linear correlation between the Phi (PSII) and the P-sat (r = 0.918). The results indicate that with the senescence of the flag leaves of winter wheat the photosynthetic efficiency including that of the energy transport and the CO2 assimilation significantly decreased. 展开更多
关键词 winter wheat flag leave fluorescence kinetics PHOTOSYNTHESIS
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FLAG方案治疗难治复发急性髓系白血病的临床研究 被引量:21
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作者 王利军 丁洁 +4 位作者 朱成英 杨华 王红新 靖彧 于力 《中国实验血液学杂志》 CAS CSCD 北大核心 2016年第1期19-24,共6页
目的:通过回顾性分析FLAG治疗方案对初治诱导失败和难治复发急性髓系白血病(acute myeloid leukemia,AML)患者的疗效及患者预后,探讨患者的治疗选择及治疗时机。方法:对2006年7月至2013年7月住院治疗的38名初治诱导失效及难治/反复AML... 目的:通过回顾性分析FLAG治疗方案对初治诱导失败和难治复发急性髓系白血病(acute myeloid leukemia,AML)患者的疗效及患者预后,探讨患者的治疗选择及治疗时机。方法:对2006年7月至2013年7月住院治疗的38名初治诱导失效及难治/反复AML患者进行了回顾性分析。38例患者的中位年龄40.5(13-69)岁,根据疾病状态分为初次诱导失败组、再次诱导失败组、早期复发组、晚期复发组;根据危险分层分为预后良好、预后中等和预后不良组。结果:22例达CR(complete remission),5例达PR(partial remission),CR率为57.9%,总有效率OR(overall response)为71.1%。初次诱导失败组的CR率(12/16,75%)高于再次诱导失败组(3/7,42.9%)和晚期复发组(6/12,50%),预后较好组和预后中等组的CR率(5/8,62.5%,16/26,61.5%)高于预后不良组(1/4,25%)。危险分层与CR有关(P=0.03)[OR 25.9,(95%CI 1.2-545.4)],预后中等是CR的有利因素。在22例CR患者中,12例行造血干细胞移植,其中6例存活,另10例以大剂量阿糖胞苷和其它方案进行巩固治疗。总生存期为25月。单因素分析FLAG方案治疗反应与生存相关[HR=0.246,CR vs NR(95%CI 0.07-0.79)],(P=0.03),CR组和PR组2年累积生存率分别为62%和48%。预后良好、预后中等和预后不良组18个月累积生存率为73%、52%和36%(P=0.17);初次诱导失败组和再次诱导失败组18月累积生存率分别为65%和32%(P=0.19)。结论:FLAG方案治疗难治复发AML患者疗效确切,通过化疗获得缓解的患者可进一步采用造血干细胞移植或其它方案以巩固化疗效果和延长生存。 展开更多
关键词 急性髓系白血病 FLAG方案 难治复发
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FLAG方案治疗初次诱导失败的急性髓系白血病的疗效观察 被引量:15
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作者 王书红 于力 +3 位作者 王全顺 李红华 赵瑜 李菲 《中国实验血液学杂志》 CAS CSCD 2007年第6期1297-1299,共3页
为了探讨FLAG方案治疗初次诱导失败的急性髓系白血病的疗效,本研究采用FLAG方案(氟达拉宾50mg,静脉点滴,30分滴完,第1-5天;阿糖胞苷1000mg/m2,静脉点滴,第1-5天;G-CSF300μg皮下注射第0-5天)治疗19例初次诱导失败的急性髓系白血病。结... 为了探讨FLAG方案治疗初次诱导失败的急性髓系白血病的疗效,本研究采用FLAG方案(氟达拉宾50mg,静脉点滴,30分滴完,第1-5天;阿糖胞苷1000mg/m2,静脉点滴,第1-5天;G-CSF300μg皮下注射第0-5天)治疗19例初次诱导失败的急性髓系白血病。结果表明:19例患者中,13例达完全缓解(CR率68.4%),2例达部分缓解(PR率10.5%),总有效率78.9%。CR的患者中有5例已行异基因造血干细胞移植,目前3例均无病存活,其中存活最长的为26个月,仍处于CR期。该治疗方案毒副作用主要为骨髓抑制、中性粒细胞减少、消化道症状、轻度肝功能异常。结论:FLAG方案对初次诱导失败的急性髓系白血病疗效好,患者对毒副作用可以耐受。因此,对于初次诱导失败的急性髓系白血病患者应该及早应用FLAG方案,为患者行造血干细胞移植创造条件。 展开更多
关键词 FLAG方案 急性髓系白血病 造血干细胞移植
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FLAG联合改良BUCY预处理方案的异基因造血干细胞移植治疗难治复发恶性血液病疗效分析 被引量:8
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作者 王志红 吴晓雄 +7 位作者 刘周阳 马健 曹永彬 徐丽昕 吴亚妹 刘蓓 闫蓓 达万明 《中国实验血液学杂志》 CAS CSCD 2011年第1期158-162,共5页
本研究旨在观察应用FLAG联合改良的BUCY预处理方案行异基因造血干细胞移植治疗难治复发或高危恶性血液病的疗效。对10例难治复发或高危恶性血液病患者自愿接受FLAG联合改良的BUCY预处理的异基因造血干细胞移植的疗效进行分析。10例中复... 本研究旨在观察应用FLAG联合改良的BUCY预处理方案行异基因造血干细胞移植治疗难治复发或高危恶性血液病的疗效。对10例难治复发或高危恶性血液病患者自愿接受FLAG联合改良的BUCY预处理的异基因造血干细胞移植的疗效进行分析。10例中复发2例(早期复发1例,淋巴瘤进展1例),难治7例(2例CR2,1例CR3,2例PR,2例NR),1例CR1但为高危病例。10例患者中包括急性髓性白血病8例(M11例,M25例,M61例,恶性骨髓增生异常综合征伴骨髓纤维化1例),前T淋巴母细胞淋巴瘤/白血病1例,脾边缘带淋巴瘤IVB1例。结果表明,10例全部顺利植入,10例患者除2例死亡(1例死于肺部感染,1例死于真菌肺炎、渗漏综合征、多脏器功能衰竭),其余8例无复发,中位生存时间为164天(57-442天)。结论:应用FLAG联合改良的BUCY预处理方案(清肿瘤性方案)的异基因造血干细胞移植治疗难治复发或高危的恶性血液病可取得良好的疗效。 展开更多
关键词 异基因造血干细胞移植 FLAG方案 Bucy方案 白血病 淋巴瘤
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演化测试技术的研究 被引量:9
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作者 谢晓园 许蕾 +2 位作者 徐宝文 聂长海 史亮 《计算机科学与探索》 CSCD 2008年第5期449-466,共18页
演化测试作为一种非常有前景的自动化测试技术,可以成功地为多种测试目标生成高质量的测试用例。它将测试用例的生成过程转化为一个利用遗传算法进行数值优化的问题。算法的搜索空间即为待测软件的输入域,其中最优解即是满足测试目标的... 演化测试作为一种非常有前景的自动化测试技术,可以成功地为多种测试目标生成高质量的测试用例。它将测试用例的生成过程转化为一个利用遗传算法进行数值优化的问题。算法的搜索空间即为待测软件的输入域,其中最优解即是满足测试目标的测试用例。该搜索过程可以实现完全自动化,它所具有的导向性可以避免盲目搜索,较随机测试有着更加明确的目标和方向性;同时该搜索过程还具有一定的随机扰动,可以很好地克服复杂搜索域所带来的种种局限性。近年来,越来越多的学者开始关注于演化测试技术的研究,并取得了一些研究成果,使之在多种领域得以成功地应用。分别在结构性测试、功能性测试、性能测试以及面向对象测试领域,对演化测试技术进行了总结。通过对各领域的应用方法进行分类概括,比较分析了不同方法的优势与不足,为理解、评价以及合理选取演化测试方法提供了较有价值的参考。在此基础上,总结评估了现有的演化测试优化技术及其适用性。最后,展望了演化测试技术未来的研究方向。 展开更多
关键词 软件测试 遗传算法 演化测试 动态优化 种群早熟 flag变量
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IDA-FLAG方案治疗复发难治的急性白血病 被引量:15
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作者 钱思轩 李建勇 +4 位作者 吴汉新 张闰 洪鸣 徐卫 仇红霞 《中国实验血液学杂志》 CAS CSCD 2009年第2期464-467,共4页
本研究探讨去甲氧柔红霉素(IDA)联合FLAG方案应用于复发难治急性白血病(AL)的疗效和不良反应。IDA+FLAG方案具体为:IDA10-12mg/(m2.d),第1-3天,氟达拉滨(Flu)30mg/(m2.d)(50mg/d),第1-5天,静脉滴注;阿糖胞苷(Ara-C)2000mg/(m2.d),第1-5... 本研究探讨去甲氧柔红霉素(IDA)联合FLAG方案应用于复发难治急性白血病(AL)的疗效和不良反应。IDA+FLAG方案具体为:IDA10-12mg/(m2.d),第1-3天,氟达拉滨(Flu)30mg/(m2.d)(50mg/d),第1-5天,静脉滴注;阿糖胞苷(Ara-C)2000mg/(m2.d),第1-5天;粒细胞集落刺激因子(G-CSF)300μg/d,皮下注射,第0-5天。4例AL患者,男性,年龄32-44岁,其中急性髓细胞白血病(AML)3例,急性淋巴细胞白血病(ALL)1例,均为中大剂量阿糖胞苷巩固后复发或FLAG诱导无效。结果显示:4例接受IDA+FLAG治疗方案1个疗程后均达到完全缓解(CR)。1例进行异基因外周血干细胞移植(allo-PBSCT)后4个月后发生血栓性血小板减少性紫癜死亡,1例缓解后10个月再次复发,2例持续CR时间为3和4个月。化疗的不良反应主要有骨髓抑制和粒细胞缺乏所致的感染,未见心脏毒性反应及其它严重的非造血系统不良反应。结论:IDA联合FLAG治疗复发难治的AL有一定疗效,尤其对于接受中大剂量Ara-C为主的方案后而复发或无效的AL患者有较高的CR率;此外,患者对此治疗方案的毒副作用可以耐受,这为进一步治疗创造时机。 展开更多
关键词 难治复发急性白血病 去甲氧柔红霉素 FLAG方案
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Effects of Different Levels of Phosphorus Nutrient on the Photosynthesis Characteristic of Rice Flag Leaf 被引量:9
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作者 李跃娜 侯立刚 +5 位作者 齐春艳 孙洪娇 刘亮 隋朋举 郭晞明 赵国臣 《Agricultural Science & Technology》 CAS 2010年第6期11-14,共4页
[Objective] To provide scientific basis for high phosphorus efficiency cultivation and regulation of rice. [Method] Changbai9 (CB9) and Jijing81 (JJ81) were used as experimental materials for pot experiment, and f... [Objective] To provide scientific basis for high phosphorus efficiency cultivation and regulation of rice. [Method] Changbai9 (CB9) and Jijing81 (JJ81) were used as experimental materials for pot experiment, and five levels of phosphorus nutrient ( Po - P4 ) were set for each variety, the net photosynthesis rate, Chlorophyll Meter Readings (SPAD Readings), stomatal conductance, transpiration rate, intercellular CO2 concentration, and stomatal limitation were observed and compared between five different phosphorus levels at filling stage. [ Result] The net photosynthesis rate of CB9 reached the highest at P3, and was significantly different from other treatments (P 〈0.05);the net photosynthesis rate of groups with phosphorus of J J81 were higher than that of the control group,and reached the highest at P,. The SPAD Readings of CB9 and JJ81 all achieved the highest at P3, and stomatal conductance and transpiration rate were all at first increased then decreased with the phosphor- us increased in CB9 and JJ81. With the phosphorus increased, intercellular CO2 concentration of CB9 decreased at first and then increased, while in JJ81 the trend was inversed,but the tendency of intercellular CO2 concentration were opposite to that of stomatal limitation in both culti- vars. [ Conclusion] Appropriate adding phosphorus could improve photosynthetic characteristic of rice flag-leaf,but displayed significant genotypic difference. 展开更多
关键词 Phosphorus nutritionl RICE Flag leaf Photosynthetic characteristic
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