In vitro cell loading experiments are used to investigate stimulation of strain to cellular proliferation. As the flowing conditions of culture fluid in loading systems has been little known, strictly people can not d...In vitro cell loading experiments are used to investigate stimulation of strain to cellular proliferation. As the flowing conditions of culture fluid in loading systems has been little known, strictly people can not detect the influence of strain to cellular proliferation exactly because shear flow can enhance cell proliferation either. Based on the working principle and cyclic loading parameters, we simplify Navier-Stokes equation to describe the flow of culture fluid on substrates of uniaxial and equi-biaxial flat tensile loading systems and four point bending system. With approximate solutions, the distributions of velocity field and wall shear flow to cells are gained. Results show: shear flows are zero in the middle (or fixed point or line) of substrate for all systems, and they get larger proportionally to distance from middle and substrate elongate; the shear flow on the substrate of four point bending system is much greater than those of others. This shear flow in four point bending system, confirmed by Owan, I., et al with OPN mRNA increase in their experiment, could cause more influence to osteoblast-like cells than that caused by strain. We estimate the average magnitude of shear stress in Owan’s device, the results are consistent with other experimental data about shear flow. In conclusion our study makes it possible to differentiate the influence of strain on cellular proliferation to that of shear flow in loading experiments with the devices mentioned above quantitatively.展开更多
提出了一种在AN SY S/LS-DYNA中计算舰船水下非接触爆炸冲击动响应的方法,即用平断面加载法模拟冲击波载荷,利用AN SY S/LS-DYNA自带的解释性语言APDL编制相应的加载程序。讨论了加载面的形式对流场网格划分和压力传播的影响。算例结果...提出了一种在AN SY S/LS-DYNA中计算舰船水下非接触爆炸冲击动响应的方法,即用平断面加载法模拟冲击波载荷,利用AN SY S/LS-DYNA自带的解释性语言APDL编制相应的加载程序。讨论了加载面的形式对流场网格划分和压力传播的影响。算例结果表明,使用该子程序进行加载能够有效改善软件在计算水中冲击波压力时压力峰值衰减过快的不足,船体结构的响应与资料中给出的试验值接近。该方法使用范围比较广,并能大幅提高计算速度,对相关理论研究和工程计算具有一定参考价值。展开更多
文摘In vitro cell loading experiments are used to investigate stimulation of strain to cellular proliferation. As the flowing conditions of culture fluid in loading systems has been little known, strictly people can not detect the influence of strain to cellular proliferation exactly because shear flow can enhance cell proliferation either. Based on the working principle and cyclic loading parameters, we simplify Navier-Stokes equation to describe the flow of culture fluid on substrates of uniaxial and equi-biaxial flat tensile loading systems and four point bending system. With approximate solutions, the distributions of velocity field and wall shear flow to cells are gained. Results show: shear flows are zero in the middle (or fixed point or line) of substrate for all systems, and they get larger proportionally to distance from middle and substrate elongate; the shear flow on the substrate of four point bending system is much greater than those of others. This shear flow in four point bending system, confirmed by Owan, I., et al with OPN mRNA increase in their experiment, could cause more influence to osteoblast-like cells than that caused by strain. We estimate the average magnitude of shear stress in Owan’s device, the results are consistent with other experimental data about shear flow. In conclusion our study makes it possible to differentiate the influence of strain on cellular proliferation to that of shear flow in loading experiments with the devices mentioned above quantitatively.
文摘提出了一种在AN SY S/LS-DYNA中计算舰船水下非接触爆炸冲击动响应的方法,即用平断面加载法模拟冲击波载荷,利用AN SY S/LS-DYNA自带的解释性语言APDL编制相应的加载程序。讨论了加载面的形式对流场网格划分和压力传播的影响。算例结果表明,使用该子程序进行加载能够有效改善软件在计算水中冲击波压力时压力峰值衰减过快的不足,船体结构的响应与资料中给出的试验值接近。该方法使用范围比较广,并能大幅提高计算速度,对相关理论研究和工程计算具有一定参考价值。
