A simple,rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis(C.sinensis)a natural plant with anti-hyperglycemic effect,was developed and validated by reversed phase liquid chrom...A simple,rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis(C.sinensis)a natural plant with anti-hyperglycemic effect,was developed and validated by reversed phase liquid chromatography.Chromatographic separation was achieved on a C18 column and an isocratic elution was carried out at a flow-rate of 1.0 mL·min^(-1) with the acetonitrile-water containing0.1%formic acid(19∶81,v/v).The detection wavelength was set at 330 nm.The calibration curve was linear over the concentration range of 0.25-50μg·mL^(-1) with correlation coefficients larger than0.999 5.The limit of detection and limit of quantification were 0.09μg·mL^(-1)and 0.25μg·mL^(-1),respectively.The precisions and accuracy for all samples were acceptable.The validated method has been successfully applied for the quantification of camellianin B in C.sinensis harvested in different months and may also be used as the quality evaluation of this herb medicine.展开更多
A new flavone glycoside, 5,8-dihydroxy-4’,6,7-trimethoxyflavone 8-O-(-D-glucopyranoside 1, together with three known flavonoids, pedalitin 2, cirsimartin 3 and genkwanin 4, were isolated from the aerial parts of Isod...A new flavone glycoside, 5,8-dihydroxy-4’,6,7-trimethoxyflavone 8-O-(-D-glucopyranoside 1, together with three known flavonoids, pedalitin 2, cirsimartin 3 and genkwanin 4, were isolated from the aerial parts of Isodon enanderianus. Their structures were determined on the basis of spectral data.展开更多
HPLC method for analysis of the flavonoids from ginkgo biloba extract (GBE) was studied. By suitable selection of columns, symmetrical chromatographic peaks were obtained without using acidic modifier in the mobile ph...HPLC method for analysis of the flavonoids from ginkgo biloba extract (GBE) was studied. By suitable selection of columns, symmetrical chromatographic peaks were obtained without using acidic modifier in the mobile phase, which can eliminate the time for cleaning the chromatographic system and simplify the analystic method for GBE. Experimental conditions: column: Hypersil BDS C18,5mm×4×250 mm; column temperature: 35C; mobile phase: 46% methanol-54% water; flow rate: 0.7 mL/min; detection wavelength: 360nm.展开更多
基金Supported by the Opening Foundation of the State Key Laboratory of Space Medicine Fundamentals and Application,Chinese Astronaut Research and Training Center(SMFA15K04)
文摘A simple,rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis(C.sinensis)a natural plant with anti-hyperglycemic effect,was developed and validated by reversed phase liquid chromatography.Chromatographic separation was achieved on a C18 column and an isocratic elution was carried out at a flow-rate of 1.0 mL·min^(-1) with the acetonitrile-water containing0.1%formic acid(19∶81,v/v).The detection wavelength was set at 330 nm.The calibration curve was linear over the concentration range of 0.25-50μg·mL^(-1) with correlation coefficients larger than0.999 5.The limit of detection and limit of quantification were 0.09μg·mL^(-1)and 0.25μg·mL^(-1),respectively.The precisions and accuracy for all samples were acceptable.The validated method has been successfully applied for the quantification of camellianin B in C.sinensis harvested in different months and may also be used as the quality evaluation of this herb medicine.
文摘A new flavone glycoside, 5,8-dihydroxy-4’,6,7-trimethoxyflavone 8-O-(-D-glucopyranoside 1, together with three known flavonoids, pedalitin 2, cirsimartin 3 and genkwanin 4, were isolated from the aerial parts of Isodon enanderianus. Their structures were determined on the basis of spectral data.
文摘HPLC method for analysis of the flavonoids from ginkgo biloba extract (GBE) was studied. By suitable selection of columns, symmetrical chromatographic peaks were obtained without using acidic modifier in the mobile phase, which can eliminate the time for cleaning the chromatographic system and simplify the analystic method for GBE. Experimental conditions: column: Hypersil BDS C18,5mm×4×250 mm; column temperature: 35C; mobile phase: 46% methanol-54% water; flow rate: 0.7 mL/min; detection wavelength: 360nm.
