The Protective Effects of Flavonoids from Scutellaria Baicalensis Georgi Stems and Leaves on Oligodendrocyte Damage Induced by Aβ1-42

Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ1-42-induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by Aβ1-42, an injury model was established by subjecting OLN-93 cells to Aβ1-42 exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ1-42. The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate Aβ1-42-induced damage of OL.

Mechanisms underlying attenuation of apoptosis of cortical neurons in the hypoxic brain by flavonoids from the stems and leaves of Scutellaria baicalensis Georgi

Flavonoids from the stems and leaves of Scutellaria baicalensis Georgi, an antioxidant, markedly improve memory impairments and neuronal injuries. In the present study, primary cortical neurons of rats were exposed to potassium cyanide to establish a model of in vitro neural cell apoptosis. Inhibition of apoptosis by flavonoids from the stems and leaves of Scutellaria baical- ensis Georgi at concentrations of 18.98, 37.36, and 75.92 gg/mL was detected using this model. These flavonoids dramatically increased cell survival, inhibited cell apoptosis and excessive pro- duction of malondialdehyde, and increased the activities of superoxide dismutase, glutathione peroxidase, and Na＋-K＊-ATPase in primary cortical neurons exposed to potassium cyanide. The flavonoids from the stems and leaves of Scutellaria baicalensis Georgi were originally found to have a polyhydric structure and to protect against cerebral hypoxia in in vitro and in vivo models, including hypoxia induced by potassium cyanide or cerebral ischemia. The present study suggests that flavonoids from the stems and leaves of Scutellaria baicalensis Georgi exert neuroprotective effects via modulation of oxidative stress, such as malondialdehyde, superoxide dismutase, glutathione peroxidase and Na＋-K＋-ATPase disorders induced by potassium cyanide.

Flavonoids from the stems and leaves of Scutellaria baicalensis Georgi attenuate H_2O_2-induced oxidative damage to rat cortical neurons

Primary cultures of rat cortical neurons were treated with H2O2 in an in vitro model of free radical neurotoxicity. Flavonoids extracted from the stems and leaves of Scutellaria baicalensis Georgi, known as SSF, at concentrations of 18.98, 37.36 and 75.92 μg/mL, protected neurons against H2O2 injury in a dose-dependent manner. SSF increased cell survival, reduced lactate dehydrogenase release and inhibited malondialdehyde production. SSF also inhibited reductions in superoxide dismutase, glutathione peroxidase and Na＋-K＋-ATPase activities. These results in-dicate that SSF can protect rat cortical neurons against H2O2-induced oxidative injury.

Inhibition of Flavonoids from Scutellaria baicalensis Stems and Leaves against Abnormal Formation of PHF and Regulation of PP in OA Induced Brain Injury in Rats

[Objectives] To explore the inhibitory effects of flavonoids from Scutellaria baicalensis Georgi stems and leaves against abnormal formation of PHF and regulation mechanism of protein phosphatase(PP) in okadaic acid(OA) induced brain injury in rats.[Methods]Male Sprague-Dawley rats were treated with OA(200 ng/kg) in the lateral ventricle to establish a memory impairment model,and Morris water maze was used to screen the memory impairment model.Rats were intragastrically administered with 25,50 and 100 mg/kg SSF daily for 36 d.The protein expressions of PHF,PP1,PP2A-Cα,PP2A-Cβ,PP2 CA and PP2 CB in rat cortex and hippocampus were determined by Western blot.Ginkgo biloba leaf flavonoids were used as positive control drugs.[Results]Compared with the sham-operated rats,the expression of PHF protein in the cortex and hippocampus of the model group was significantly increased(P < 0.01);protein expression of PP2A-Cα and PP2A-Cβin the cortex and hippocampus,and PP2 CB in the hippocampus were significantly decreased(P < 0.05);protein expression of PP2 CA and PP2 CB in the cortex was significantly increased(P < 0.01);protein expression of PP1 in the cortex was significantly decreased(P < 0.01).Compared with the model rats,25,50 and 100 mg/kg SSF 36 d reversed the protein expression of PHF,PP2A-Cα and PP2ACβ in rat cortex and hippocampus and expression of PP1 in rat cortex induced by OA,but had no significant effect on the protein expression of PP2 CA and PP2 CB,G.biloba flavonoids also showed similar results to SSF.[Conclusions] SSF can significantly inhibit the abnormal formation of PHF in rat brain induced by OA.The inhibitory effect may be related to the regulation of PP1,PP2A-Cα and PP2A-Cβ protein expression in cerebral cortex and hippocampus by SSF,but less related to the regulation of PP2 CA and PP2 CB.

Pharmacological Activity and Application of Roots, Stems and Leaves of Scutellaria baicalensis Georgi

The root of Scutellaria baicalensis Georgi is traditionally used as medicine,and it has been confirmed that S.baicalensis Georgi has flavonoid chemical constituents,pharmacological activity and cosmetic efficacy.With the extensive application of S.baicalensis Georgi roots,the resource of S.baicalensis Georgi has been increasingly short.The above-ground part of stems and leaves of S.baicalensis Georgi has also been gradually recognized and developed.Studies have found that the chemical constituents from stems and leaves of S.baicalensis Georgi are also a group of flavonoids with a lot of pharmacological activity and have a great application value.Based on this,the present review will be reported on the chemical constituents and application of the roots,stems and leaves of S.baicalensis Georgi.

Pretreatment with scutellaria baicalensis stem-leaf total flavonoid protects against cerebral ischemia/reperfusion injury in hippocampal neurons

Previous experimental studies have shown that cerebral infarction can be effectively reduced following treatment with scutellaria baicalensis stem-leaf total flavonoid （SSTF）. However, the mechanism of action of SSTF as a preventive drug to treat cerebral infarction remains unclear. In this study, Sprague-Dawley rats were pretreated with 50, 100, 200 mg/kg SSTF via intragastric ad- ministration for 1 week prior to the establishment of focal cerebral ischemia/reperfusion injury. The results showed that pretreatment with SSTF effectively improved neurological function, reduced brain water content and the permeability of blood vessels, ameliorated ischemia-induced morphology changes in hippocampal microvessels, down-regulated Fas and FasL protein expression, elevated the activity of superoxide dismutase and glutathione peroxidase, and decreased malondialdehyde content. In contrast to low-dose SSTF pretreatment, the above changes were most obvious after pretreatment with moderateand high-doses of SSTF. Experimental findings indicate that SSTF pretreatment can exert protective effects on the brain against cerebral ischemia/reperfusion injury. The underlying mechanisms may involve reducing brain water content, increasing microvascular recanalization, inhibiting the apoptosis of hippocampal neurons, and attenuating free radical damage.

Pretreatment with scutellaria baicalensis stem-leaf total flavonoid prevents cerebral ischemia-reperfusion injury

Pretreatment with scutellaria baicalensis stem-leaf total flavonoid has protective effects against ischemia and attenuates myocardial ischemia-reperfusion injury. In this study, rats were given scutellaria baicalensis stem-leaf total flavonoid intragastrically at 50, 100, and 200 mg/kg per day for 7 days before focal cerebral ischemia-reperfusion injury models were established using the suture method. We then determined the protective effects of scutellaria baicalensis stem-leaf total flavon- oid pretreatment on focal cerebral ischemia-reperfusion injury. Results showed that neurological deficit scores increased, infarct volumes enlarged, apoptosis increased and Bcl-2 and Bax protein expression were upregulated at 24 hours after reperfusion. Pretreatment with scutellaria baicalensis stem-leaf total flavonoid at any dose lowered the neurological deficit scores, reduced the infarct volume, prevented apoptosis in hippocampal cells, attenuated neuronal and blood-brain barrier damage and upregulated Bcl-2 protein expression but inhibited Bax protein expression. Doses of 100 and 200 mg/kg were the most efficacious. Our findings indicate that pretreatment with scutel- laria baicalensis stem-leaf total flavonoid at 100 and 200 mg/kg can improve the neurological func- tions and have preventive and protective roles after focal cerebral ischemia-reperfusion injury.

Simultaneous determination of 15 flavonoids from different parts of Scutellaria baicalensis and its chemometrics analysis

Objective: The aerial parts of Scutellaria baicalensis were used as Huangqin Tea for thousands of years and mainly contain flavonoids which contribute to its bioactivities. However, there is no appropriate quality evaluation method of Huangqin Tea, and three flavanones of isocarthamidin-7-O-β-D-glucuronide,carthamidin-7-O-β-D-glucuronide, and isoscutellarein-8-O-β-D-glucuronide with high contents in the aerial parts have never been defined quantitatively. Here, an HPLC-DAD method for simultaneous determination of 15 flavonoids and systematically compared their contents and distribution in the roots,stems, leaves, and flowers of S. baicalensis was established.Methods: Under the HPLC-DAD chromatographic conditions, 77 batches of samples of S. baicalensis were analyzed. Meanwhile, the chromatographic fingerprint of different parts of S. baicalensis was established.Subsequently, principal component analysis(PCA), orthogonal projections to latent structures discriminant analysis(OPLS-DA), and clustering heat map were performed based on the contents of 15 flavonoids in different parts of S. baicalensis.Results: The results showed significant differences in the contents and distributions of 15 flavonoids among the different parts of S. baicalensis. The chemical composition of stems showed some similarities to leaves, and their contents were all lower than leaves. The contents of isocarthamidin-7-O-β–glucuronide [(106.66 66 ± 22.68) mg/g], carthamidin-7-O-β-D-glucuronide [(19.82 ± 11.17) mg/g],and isoscutellarein-8-O-β-D-glucuronide [(3.10 ± 1.73) mg/g] were the highest in leaves. The content of apigenin-7-O-β-D-glucopyranoside and chrysin-7-O-β-D-glucuronide were the highest in flowers. The contents of baicalin, baicalein, wogonoside, wogonin, alpinetin, and oroxylin A were higher in roots than in other parts.Conclusion: The method was fully validated and could be effectively used to characterize the contents and distributions of main flavonoids in the different parts of S. baicalensis. It may lay a foundation to establish the quality evaluation system for Huangqin Tea.

Virtual Evaluation on Activities of Flavonoids from Scutellaria baicalensis

Objective To explore the investigation method of complicated and profound traditional Chinese herbal medicine,the potential action mechanisms of flavonoids from Scutellaria baicalensis were studied by docking calculation.Methods In total,eight flavonoids(aglycones and their glicosides) from S.baicalensis were selected as ligands.The crystalline structures of targets related to common diseases were used as the receptors for calculation.The calculations were conducted with Schrdinger software package.The grading standard of selectivity was developed according to G-score between ligands and receptors.Results Twenty-six pharmacologic actions have been reported.Among all effects in literature,nine of them can be deduced from the docking calculation of aglycone.From glycosides with grade ++,25 reported effects can be estimated by calculation.Apparently,the target selectivity of aglycones and their glycosides are different form the virtual evaluation.The virtual evaluation results of glycosides were closer to the reported effects.Conclusion Our proposed virtual evaluation method seems an effective way to investigate the complicated system of traditional Chinese herbal medicine.It suggests that aglycones may be effective as the form of glucoside in vivo,and metabolism is a very important factor for virtual evaluation.

黄芩茎叶发酵黄酮的体外抑菌及抗炎作用研究

试验旨在探究黄芩茎叶发酵黄酮的抗菌抗炎效果,以大肠杆菌、沙门氏菌、志贺氏菌和金黄色葡萄球菌为试验菌种,测定最小抑菌浓度(MIC)、最小杀菌浓度(MBC)及抑菌圈大小。50只SPF级昆明小鼠随机分为5组,每组10只,除对照组(Con组)各组小鼠均腹腔注射脂多糖(LPS,4 mg/kg),Con组灌胃相同剂量的生理盐水,阳性药物组(LPS+DM组)灌喂0.5 mg/kg地塞米松,原药组(LPS+SBSL组)和发酵药物组(LPS+FSBSL组)灌喂8 mg/kg原药和发酵黄酮。试验期7 d。结果显示,发酵黄酮MIC、MBC值最低的分别是大肠杆菌和金黄色葡萄球菌;在250 g/L条件下,发酵黄酮对大肠杆菌、痢疾志贺氏菌和金黄色葡萄球菌具有中等敏感性。与LPS组相比,LPS+FSBSL组小鼠死亡率明显降低;LPS+SBSL组小鼠肝脏指数极显著高于LPS+FSBSL组(P<0.01),LPS组脾脏指数极显著高于Con组(P<0.01)。与LPS组比,LPS+DM组和LPS+SBSL组小鼠血清白细胞介素-6(IL-6)含量极显著升高(P<0.01);与LPS+DM组比,LPS+SBSL组和LPS+FSBSL组小鼠血清IL-6含量极显著升高(P<0.01)。与Con组比,LPS组小鼠血清肿瘤坏死因子-α(TNF-α)含量显著升高(P<0.05)。与LPS组相比,LPS+FSBSL组小鼠肺脏组织中IL-6含量显著升高(P<0.05)。与LPS+SBSL组相比,LPS+FSBSL组小鼠肺脏组织中白细胞介素-1β(IL-1β)含量显著升高(P<0.05)。研究表明,黄芩茎叶发酵黄酮对痢疾志贺氏菌和金黄色葡萄球菌均有较好的抑菌效果,安全剂量为8 mg/kg,对LPS感染后小鼠的死亡率具有抑制作用,同时可以调节LPS感染后小鼠血清和肺脏中炎症因子IL-6、IL-1β及TNF-α含量。

黄芩斑枯病病原菌生物学特性及室内药剂筛选

目的:明确黄芩斑枯病病原菌多主棒孢Corynespora cassiicola的生物学特性,筛选高效安全的杀菌剂,科学防治黄芩斑枯病。方法:通过菌落生长速率法评价碳源、氮源、温度、培养基、pH、光照对多主棒孢菌落生长的影响,以及5种杀菌剂对病原菌的室内毒力。结果:多主棒孢最适生长条件为胡萝卜培养基外源添加可溶性淀粉和蔗糖作为碳源、蛋白胨作为氮源、pH为6、温度为30℃。菌丝致死条件为51℃处理10 min,孢子致死条件为54℃处理10 min。98%多菌灵对多主棒孢的毒力最强,半数效应浓度(EC50)为0.07 mg·L^(-1);其次是98%咯菌腈、98%咪鲜胺和98%吡唑醚菌酯,EC50分别为0.10、0.18、3.06 mg·L^(-1);98%嘧菌酯对多主棒孢的毒力最弱,EC50为632.39 mg·L^(-1)。结论:黄芩斑枯病病原菌适生范围较广,在多种碳源、氮源、培养基及pH条件下均可生长,在中性偏弱酸、较高温条件下生长速度较快。多菌灵和咯菌腈对多主棒孢表现出较强的抑制作用,可考虑将其用于黄芩斑枯病的田间防治。

响应面法优化黄芩茎叶黄酮类成分提取工艺及其含量测定

采用单因素和响应面试验考察乙醇浓度、料液比、提取时间和提取温度对黄芩茎叶中黄酮成分提取量的影响;在此基础上,采用HPLC法测定了17个黄芩茎叶中黄酮成分的含量。结果表明,黄芩茎叶中黄酮成分的最佳提取工艺为乙醇浓度55%、料液比1∶50、提取时间60 min、提取温度50℃,应用最佳提取工艺黄酮成分提取量为24.43 mg/g;不同产地黄芩茎叶中黄酮成分的含量存在差异,含量范围为15.839~26.044 mg/g。响应面法优化黄芩茎叶中黄酮成分的提取工艺稳定、准确可靠,所建立的黄酮成分含量测定方法稳定可靠,可为黄芩茎叶的质量评价及深度开发研究提供参考。

黄芩总黄酮部位对常见水产致病菌的体外抑制作用

为寻找水产养殖领域天然、安全的抗生素替代品,探索黄芩醇提物对水产致病菌抑制作用的总黄酮部位.采用大孔树脂分离和抑菌圈法确定黄芩抗菌的总黄酮部位,采用紫外分光光度法和HPLC法分别对其进行定量和定性分析,采用琼脂稀释法测定黄芩总黄酮部位对7种水产致病菌的MIC.研究结果表明,黄芩中的黄酮类成分主要富集在30%醇洗和70%醇洗部位,并表现出明显的抑菌活性,其总黄酮含量达75.2%.黄芩总黄酮部位对其中6种水产致病菌的10个菌株均表现出较强的抑制作用,其MIC在0.36~1.43 mg/mL之间.黄芩总黄酮部位具有较广谱的抗水产致病菌作用,在水产养殖业有着广泛的应用前景.

黄芩总黄酮改善哮喘模型小鼠气道炎症及对TLR3/TRIF通路表达的影响

目的探讨黄芩总黄酮对哮喘小鼠气道炎症的改善作用及对Toll样受体3(Toll-likereceptor3,TLR3)/β干扰素TIR结构域衔接蛋白(TIRdomain-containing adaptorinducing interferon-beta,TRIF)通路表达的影响。方法随机将50只4~6周龄雌性Balb/c小鼠分为正常对照组、模型组、阳性对照组(地塞米松,1mg/kg)、黄芩总黄酮低剂量组(25mg/kg)、黄芩总黄酮高剂量组(50mg/kg),每组各10只小鼠。除正常对照组外,其余各组构建支气管哮喘(以下简称为哮喘)小鼠模型。苏木精-伊红(hematoxylin-eosinstaining,HE)染色法观察小鼠肺组织病理改变,并根据病理图片分析小鼠气道重塑情况,计算支气管壁面积/内周长(W/Pi)、支气管平滑肌面积/内周长(S/Pi)和平滑肌细胞核数/内周长(N/Pi)值。采用酶联免疫吸附试验(enzyme linked immunosorben tassay,ELISA)法检测肺泡灌洗液(bronchoalveolar lavagefluid,BALF)中白细胞介素6(interleukin6,IL-6)和IL-8水平。实时荧光定量聚合酶链式反应(quantitative real-timepolymerase chain reaction,qPCR)检测小鼠肺组织中TLR3、TRIFmRNA表达水平。Westernbolt检测小鼠肺组织中TLR3、TRIF蛋白表达水平。结果病理结果显示,正常对照组的支气管腔和肺泡均保持良好的完整性;模型组的小鼠表现出黏膜水肿、炎性细胞浸润(黏膜下层和黏膜层)以及气道壁炎性细胞浸润数量增加,给予黄芩总黄酮后小鼠支气管腔和肺泡炎性细胞浸润减少,且黄芩总黄酮高剂量组炎性细胞数少于黄芩总黄酮低剂量组。与正常对照组相比,模型组W/Pi、S/Pi、N/Pi,IL-6、IL-8,TLR3、TRIFmRNA和蛋白水平,均显著增加(P均<0.05);与模型组相比,黄芩总黄酮低、高剂量组和阳性对照组W/Pi、S/Pi、N/Pi,IL-6、IL-8,TLR3、TRIFmRNA和蛋白水平,均显著降低(P均<0.05);黄芩总黄酮低剂量组W/Pi、S/Pi、N/Pi,IL-6、IL-8,TLR3、TRIFmRNA和蛋白水平,均显著高于黄芩总黄酮高剂量组(P均<0.05);黄芩总黄酮高剂量组与阳性对照组各指标比较差异均无统计学意义(P>0.05)。结论黄芩总黄酮可有效改善哮喘模型小鼠的气道炎症,其机制可能与抑制TLR3/TRIF信号通路,降低炎症反应,缓解气道重塑有关。

黄芩茎叶总黄酮抗心律失常作用研究

目的 观察黄芩茎叶总黄酮 (以下简称总黄酮 )的抗心律失常作用。方法 采用乌头碱和冠状动脉结扎复灌诱发大鼠的室性心律失常、哇巴因诱发豚鼠的室性心律失常模型 ,记录总黄酮对乌头碱和哇巴因致室早 (VP)、室颤 (VF)、心脏停跳 (HS)的剂量和冠状动脉结扎复灌致 VP、VF及 HS发生率。结果 总黄酮 2 0 ,4 0 mg/kg显著增加乌头碱致 VP、VF及 HS的阈剂量 ,减少冠脉结扎复灌诱发大鼠 VP、VF及 HS的发生率 ;可提高哇巴因诱发豚鼠 VP、VF的阈剂量 ,但对致死剂量无明显影响。结论 总黄酮对实验性心律失常有显著的拮抗作用。

黄芩茎叶总黄酮调血脂作用研究

黄芩茎叶总黄酮 (以下简称总黄酮 )是黄芩茎叶的主要有效部位。动物实验结果证明 ,在给大鼠喂以高脂饲料的同时 po总黄酮能明显抑制血清总胆固醇 (TC)、甘油三酯 (TG)和低密度脂蛋白 (L DL- C)的升高。对已经形成高脂血症的大鼠 ,也能明显降低血清 TC、TG、L DL- C的水平及动脉粥样硬化指数 (AI,AI=TC-HDL- C/ HDL- C)。

黄芩类黄酮对人免疫细胞化学发光及淋巴细胞增殖的影响

目的:探讨黄芩类黄酮对人免疫细胞影响。方法:检测了从黄芩中纯化的3′,5,6,7四羟基2′,8二甲氧基黄酮、2′,5二羟基6,6′,7,8四甲氧基黄酮、5,7,8三羟基黄酮、(2R,3R)2′,3,5,6′,7五羟基黄烷醇、黄芩甙和黄芩酮6种类黄酮成分对人多形核细胞(PMN)、单个核细胞(MNC)化学发光反应和PHA诱导淋巴细胞增殖的影响。结果:除(2R,3R)2′,3,5,6′,7五羟基黄烷醇外,其它5种抑制NFomylMetLeuPhe(fMLP)激活的PMN煌MNC或调理酵母多糖(OZ)激活的PMN产生的化学发光,也显著地抑制PHA诱导的淋巴细胞增殖,在25×10-4mol/L浓度时抑制化学发光的抑制率可达41%～99%。抑制淋巴细胞增殖的抑制率可达89%～99%。结论:从黄芩中分离的类黄酮对PMN、MNC和淋巴细胞有不同的作用。这些作用可能与黄芩的抗炎症和抗过敏作用有关系。也显示这些成分有可能成为新的抗炎或免疫抑制药物。

黄芩茎叶总黄酮治疗2型糖尿病性高脂血症大鼠的实验研究

目的观察黄芩茎叶总黄酮(SSTF)对2型糖尿病性高脂血症大鼠血糖、血脂代谢及胰岛素抵抗的影响,探究其可能的作用机制。方法高脂饮食加小剂量链脲佐菌素(STZ)建立2型糖尿病合并高脂血症大鼠模型,观察SSTF对大鼠血糖、血脂、胰岛素敏感性指数、抗氧化能力等方面的影响。结果SSTF大、小剂量组(150,75mg·kg-1)能显著降低实验大鼠空腹血糖(FBG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平,不同程度的降低甘油三酯(TG)水平,显著升高高密度脂蛋白胆固醇(HDL-C)水平和胰岛素敏感性指数(ISI)。SSTF能显著降低血清和肝脏中丙二醛(MDA)的含量,显著增加血清超氧化物歧化酶(SOD)的活性,不同程度地提高血清谷胱甘肽(GSH)含量。结论黄芩茎叶总黄酮降糖、降脂作用确切,并能改善胰岛素抵抗作用。SSTF的作用机制可能和其显著的抗氧化作用有关。

黄芩黄酮对自由基引起的大鼠脑线粒体损伤的保护作用

目的 探讨黄芩中主要四种黄酮—黄芩素、黄芩苷、汉黄芩素和汉黄芩苷对脑线粒体氧化损伤的保护作用。方法 运用抗坏血酸 Fe2 +、2 ,2 azobis ( 2 amidinopropane)hy drochloride (AAPH)及NADPH三种不同损伤体系诱导大鼠大脑皮质线粒体脂质过氧化 ,研究黄芩中四种主要黄酮在不同体系中的保护作用。结果 四种黄酮在 10 μmol·L-1下对NADPH诱导的脂质过氧化都具有保护作用 ,而只有黄芩素和黄芩苷在前二体系中效果显著 ;四种黄酮对抗坏血酸 Fe2 +体系诱发的线粒体肿胀和膜的流动性降低都有一定的保护作用。结论 黄芩黄酮能抑制不同体系诱导的线粒体氧化损伤 。

黄芩茎叶总黄酮预处理对缺血再灌注心肌脂质过氧化损伤的保护作用

目的：探讨黄芩茎叶总黄酮(SSTF)预处理对缺血再灌注心肌脂质过氧化损伤的保护作用。方法：雄性大鼠分为SSTF预处理组、缺血再灌组和假手术组,缺血再灌术前给SSTF组大鼠SSTF(50、100、200mg·kg^1·d^(-1))灌胃,制备缺血再灌模型,分别测定心肌组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,血清乳酸脱氢酶(LDH)、肌酸激酶(CK)水平。结果：SSTF预处理可不同程度显著降低缺血再灌注引起的MDA含量,提高SOD活性,降低LDH、CK水平。结论：SSTF预处理能通过保护心肌抗氧化酶的活性,抵制脂质过氧化反应,减轻自由基对心肌的损害,对缺血再灌注心肌产生保护作用。