Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods:...Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering dangshen.Results: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the recovery.Conclusion: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.展开更多
[Objective] The paper was to understand the physiological response of space flight mutation new strains of Festuca arundinacea to high temperature stress. [Method] The influence of high temperature stress on eco-physi...[Objective] The paper was to understand the physiological response of space flight mutation new strains of Festuca arundinacea to high temperature stress. [Method] The influence of high temperature stress on eco-physiological characteristics of 11 F. arundinacea materials was studied in a pot experiment.Physiological and biochemical indexes,including soluble protein content,superoxide dismutase( SOD),peroxidase( POD) and catalase( CAT) activities,were measured and analyzed once every 4 d for a total of three times. Subordinate function and grey rational analysis were used to comprehensively evaluate the heat resistance of 11 materials. [Result] The soluble protein content decreased with the extension of heat resistance stress,while SOD,POD and CAT activities showed an upward trend. The high temperature resistance of 11 F. arundinacea materials was evaluated by subordinate function method. The resistance order was SP5-85> SP5-60 > SP5-71 > SP5-42 > SP5-94 > SP5-7 > SP5-88 > F. arundinacea cv Shuicheng > SP5-5 > SP5-89 = F. arundinacea cv Qiancao No. 1. The relational order of various heat resistance indexes and heat resistance obtained by grey rational analysis was SOD > CAT > POD > soluble protein content. [Conclusion]The result provides a theoretical basis for stress physiology and stress breeding of cold season grass in southwest region.展开更多
YK- 1101, with its structure as S-（（E）-4-（（7S, 10S,Z）-4-ethylidene-7-isopropyl-2,5,8,12- tetraoxo -9-oxa- 16-thia-3,6,13,18-tetraazabicyclo[ 13.2.1 ]octadeca- 1 （17）, 15（ 18）-dien- 10-yl）but-3-en- 1 -y l） ...YK- 1101, with its structure as S-（（E）-4-（（7S, 10S,Z）-4-ethylidene-7-isopropyl-2,5,8,12- tetraoxo -9-oxa- 16-thia-3,6,13,18-tetraazabicyclo[ 13.2.1 ]octadeca- 1 （17）, 15（ 18）-dien- 10-yl）but-3-en- 1 -y l） ethanethioate, is synthesized as a potential histone deacetylase inhibitor. Its quality and stability under various stress conditions are not fully understood. In this study, a high performance liquid chromatographic （HPLC） method was established and validated for the analysis of YK-1101 bulk drug samples. The chromatographic separation was performed on a C18 column with acetonitrile and water as mobile phase in a gradient elution. Based on the established method, the stability studies of YK-1101 under various stress conditions were carried out. YK-1101 was shown to undergo degradation under basic and acidic stress conditions, while it was stable under oxidative, photolytic and thermal conditions. In addition~ a time of flight mass spectrometer （TOF/MS） was coupled to HPLC for the characterization of major degradation products produced under basic and acidic stress conditions. Their degradation pathways were also discussed.展开更多
基金Guangdong Science and Technology Department,Grant/Award Number:2015A030302073
文摘Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering dangshen.Results: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the recovery.Conclusion: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.
基金Supported by United Fund of Guizhou Province(QKH J LKN[2013]24)Key Special Project of Guizhou Province(QKHZDZX[2014]6017)High-level Innovative Talents Training of Guizhou Province(QKHRC[2016]4024)
文摘[Objective] The paper was to understand the physiological response of space flight mutation new strains of Festuca arundinacea to high temperature stress. [Method] The influence of high temperature stress on eco-physiological characteristics of 11 F. arundinacea materials was studied in a pot experiment.Physiological and biochemical indexes,including soluble protein content,superoxide dismutase( SOD),peroxidase( POD) and catalase( CAT) activities,were measured and analyzed once every 4 d for a total of three times. Subordinate function and grey rational analysis were used to comprehensively evaluate the heat resistance of 11 materials. [Result] The soluble protein content decreased with the extension of heat resistance stress,while SOD,POD and CAT activities showed an upward trend. The high temperature resistance of 11 F. arundinacea materials was evaluated by subordinate function method. The resistance order was SP5-85> SP5-60 > SP5-71 > SP5-42 > SP5-94 > SP5-7 > SP5-88 > F. arundinacea cv Shuicheng > SP5-5 > SP5-89 = F. arundinacea cv Qiancao No. 1. The relational order of various heat resistance indexes and heat resistance obtained by grey rational analysis was SOD > CAT > POD > soluble protein content. [Conclusion]The result provides a theoretical basis for stress physiology and stress breeding of cold season grass in southwest region.
基金supported by the postgraduate innovation foundation of Simcere (No. CX11S-002XS)
文摘YK- 1101, with its structure as S-（（E）-4-（（7S, 10S,Z）-4-ethylidene-7-isopropyl-2,5,8,12- tetraoxo -9-oxa- 16-thia-3,6,13,18-tetraazabicyclo[ 13.2.1 ]octadeca- 1 （17）, 15（ 18）-dien- 10-yl）but-3-en- 1 -y l） ethanethioate, is synthesized as a potential histone deacetylase inhibitor. Its quality and stability under various stress conditions are not fully understood. In this study, a high performance liquid chromatographic （HPLC） method was established and validated for the analysis of YK-1101 bulk drug samples. The chromatographic separation was performed on a C18 column with acetonitrile and water as mobile phase in a gradient elution. Based on the established method, the stability studies of YK-1101 under various stress conditions were carried out. YK-1101 was shown to undergo degradation under basic and acidic stress conditions, while it was stable under oxidative, photolytic and thermal conditions. In addition~ a time of flight mass spectrometer （TOF/MS） was coupled to HPLC for the characterization of major degradation products produced under basic and acidic stress conditions. Their degradation pathways were also discussed.
