[Objective] The paper was to establish a HPLC method for quantitative determination of 80% pymetrozine·nitenpyram WG. [Method] With the mixture of acetonitrile, water and phosphate buffer （volume ratio 10：80...[Objective] The paper was to establish a HPLC method for quantitative determination of 80% pymetrozine·nitenpyram WG. [Method] With the mixture of acetonitrile, water and phosphate buffer （volume ratio 10：80：10） as the mobile phase, pymetrozine and nitenpyram in samples were separated by ODS-2 reversed phase column, and detected by UV detector at 250 nm. In addition, the results were quantified by external standard method. [Result] The linear correlation coefficients of pymetrozine and nitenpyram were 0.9995 and 0.9998; the standard deviations were 0.29 and 0.22; the coefficients of variation were 0.51% and 1.21%; and the average recoveries were 100.7% and 99.1%, respectively. [Conclusion] The method is fast and simple, and can effectively separate pymetrozine and nitenpyram, with high precision and accuracy and good linear relation.展开更多
建立了高效液相色谱法(HPLC)对7.5%甲氧磺草胺水分散粒剂(WG)进行定性和定量的分析方法。以乙腈-水(体积比98∶2)为流动相,Eclipse Plus C18为分离柱,选择215nm为紫外检测波长。试样5次测定平均值为7.55%,相对标准偏差为0.99%,添加平均...建立了高效液相色谱法(HPLC)对7.5%甲氧磺草胺水分散粒剂(WG)进行定性和定量的分析方法。以乙腈-水(体积比98∶2)为流动相,Eclipse Plus C18为分离柱,选择215nm为紫外检测波长。试样5次测定平均值为7.55%,相对标准偏差为0.99%,添加平均回收率为99.1%,标准曲线相关系数为0.9997。方法测定结果准确度和精密度高,操作简单、快速。展开更多
为了建立在同一液相色谱条件下啶氧菌酯悬浮剂及水分散粒剂中有效成分含量的通用分析方法,样品用乙腈溶解,采用反相高效液相色谱法,流动相为乙腈-水溶液(体积比80:20),流速1.0 m L/min,进样量5μL,使用Symmetry-C_(18)色谱柱和二极管阵...为了建立在同一液相色谱条件下啶氧菌酯悬浮剂及水分散粒剂中有效成分含量的通用分析方法,样品用乙腈溶解,采用反相高效液相色谱法,流动相为乙腈-水溶液(体积比80:20),流速1.0 m L/min,进样量5μL,使用Symmetry-C_(18)色谱柱和二极管阵列检测器,在波长220 nm下对试样中的啶氧菌酯进行高效液相色谱分离,外标法定量。此外,利用二极管阵列检测器进行了峰纯度检验及不同实验室方法验证。结果表明:方法的线性范围在100.1~1000.8 mg/L之间,线性相关系数R^(2)为0.9993;悬浮剂及水分散粒剂的添加回收率平均值分别为100.64%、99.74%,测定结果的相对标准偏差(RSD)值分别为1.31%、0.69%;不同实验室不同仪器之间的重复性标准偏差悬浮剂及水分散粒剂分别为0.8052%、0.6003%,再现性相对标准偏差(RSD_(R))分别为0.8560%、1.5625%。方法精密度、准确度及峰纯度结果均符合农药产品分析方法的要求,适用于啶氧菌酯悬浮剂及水分散粒剂中啶氧菌酯含量的测定。展开更多
文摘[Objective] The paper was to establish a HPLC method for quantitative determination of 80% pymetrozine·nitenpyram WG. [Method] With the mixture of acetonitrile, water and phosphate buffer （volume ratio 10：80：10） as the mobile phase, pymetrozine and nitenpyram in samples were separated by ODS-2 reversed phase column, and detected by UV detector at 250 nm. In addition, the results were quantified by external standard method. [Result] The linear correlation coefficients of pymetrozine and nitenpyram were 0.9995 and 0.9998; the standard deviations were 0.29 and 0.22; the coefficients of variation were 0.51% and 1.21%; and the average recoveries were 100.7% and 99.1%, respectively. [Conclusion] The method is fast and simple, and can effectively separate pymetrozine and nitenpyram, with high precision and accuracy and good linear relation.
文摘建立了高效液相色谱法(HPLC)对7.5%甲氧磺草胺水分散粒剂(WG)进行定性和定量的分析方法。以乙腈-水(体积比98∶2)为流动相,Eclipse Plus C18为分离柱,选择215nm为紫外检测波长。试样5次测定平均值为7.55%,相对标准偏差为0.99%,添加平均回收率为99.1%,标准曲线相关系数为0.9997。方法测定结果准确度和精密度高,操作简单、快速。
文摘为了建立在同一液相色谱条件下啶氧菌酯悬浮剂及水分散粒剂中有效成分含量的通用分析方法,样品用乙腈溶解,采用反相高效液相色谱法,流动相为乙腈-水溶液(体积比80:20),流速1.0 m L/min,进样量5μL,使用Symmetry-C_(18)色谱柱和二极管阵列检测器,在波长220 nm下对试样中的啶氧菌酯进行高效液相色谱分离,外标法定量。此外,利用二极管阵列检测器进行了峰纯度检验及不同实验室方法验证。结果表明:方法的线性范围在100.1~1000.8 mg/L之间,线性相关系数R^(2)为0.9993;悬浮剂及水分散粒剂的添加回收率平均值分别为100.64%、99.74%,测定结果的相对标准偏差(RSD)值分别为1.31%、0.69%;不同实验室不同仪器之间的重复性标准偏差悬浮剂及水分散粒剂分别为0.8052%、0.6003%,再现性相对标准偏差(RSD_(R))分别为0.8560%、1.5625%。方法精密度、准确度及峰纯度结果均符合农药产品分析方法的要求,适用于啶氧菌酯悬浮剂及水分散粒剂中啶氧菌酯含量的测定。
