The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice(Oryza sativa L.), a...The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice(Oryza sativa L.), aberrant-floral spikelet 1(afs1), which was derived from treatment of Xinong 1 B with ethyl methanesulfonate. In the afs1 mutant, the spikelet developed an additional lemma-like organ alongside the other normally developed floral organs, and the paleae were degenerated to differing degrees with or without normally developed inner floral organs. Genetic analysis revealed that the afs1 phenotype was controlled by a single recessive gene. The AFS1 gene was mapped between the insertion/deletion(In Del) marker Indel19 and the simple sequence repeat marker RM16893, with a physical distance of 128.5 kb on chromosome 4. Using sequence analysis, we identified the deletion of a 5-bp fragment and a transversion from G to A within LOC_Os04 g32510/LAX2, which caused early termination of translation in the afs1 mutant. These findings suggest that AFS1 may be a new allele of LAX2, and is involved in the development of floral organs by regulating the expression of genes related to their development. The above results provide a new view on the function of LAX2, which may also regulate the development of spikelets.展开更多
Syringa species are important ornamentals with strong floral scent,of which monoterpenes are the main component.In this study,a new monoterpene synthase gene,named SoLIM,was collected from the flowers of Syringa oblat...Syringa species are important ornamentals with strong floral scent,of which monoterpenes are the main component.In this study,a new monoterpene synthase gene,named SoLIM,was collected from the flowers of Syringa oblata and S.oblata var.alba using a homologous cloning method.The full-length cDNA of SoLIM was1746 bp and encoded 581 amino acids.Sequence analysis showed that SoLIM contained the DDxxD and RRx8 W motifs,which are two typical conserved monoterpene synthase motifs,and was thus classified as belonging to the Tpsb subfamily.Using quantitative reverse-transcription PCR,SoLIM was significantly expressed in the petals and pistils of S.oblata and S.oblata var.alba,respectively.SoLIM expression peaked earlier than the D-limonene emissions in the diurnal experiments,but occurred later when D-limonene had peaked during the flowering phase,indicating that differences in SoLIM gene expression and D-limonene emissions existed.The synthesis of floral scent is thus associated with diverse regulatory mechanisms that require further investigation.展开更多
植物FLOWERING LOCUS T/TERMINAL FLOWER1(FT/TFL1)基因家族是一个进化上高度保守的基因家族,它在植物的花发育过程中具有重要作用:其成员FT基因编码的蛋白产物是可以长距离转运的成花激素,在花形成过程中起关键作用;另一成员TFL1基因...植物FLOWERING LOCUS T/TERMINAL FLOWER1(FT/TFL1)基因家族是一个进化上高度保守的基因家族,它在植物的花发育过程中具有重要作用:其成员FT基因编码的蛋白产物是可以长距离转运的成花激素,在花形成过程中起关键作用;另一成员TFL1基因则在花序的形成和维持过程中起重要作用.本文就近年来国内外对植物FT/TFL1基因家族的结构、成员,以及各个成员在花发育转换过程中的功能等研究现状进行综述,并对该基因家族的研究前景提出展望.展开更多
基金supported by the National Natural Science Foundation of China(31900612 and 31730063)the Fundamental Research Funds for the Central Universities,China(SWU5330500322)+1 种基金the National Key Research and Development Program of China(2017YFD0100202)the Natural Science Foundation of Chongqing,China(CSTC2017jcyj BX0062)。
文摘The spikelet is a unique inflorescence structure in grasses. However, the molecular mechanism that regulates its development remains unclear, and we therefore characterize a spikelet mutant of rice(Oryza sativa L.), aberrant-floral spikelet 1(afs1), which was derived from treatment of Xinong 1 B with ethyl methanesulfonate. In the afs1 mutant, the spikelet developed an additional lemma-like organ alongside the other normally developed floral organs, and the paleae were degenerated to differing degrees with or without normally developed inner floral organs. Genetic analysis revealed that the afs1 phenotype was controlled by a single recessive gene. The AFS1 gene was mapped between the insertion/deletion(In Del) marker Indel19 and the simple sequence repeat marker RM16893, with a physical distance of 128.5 kb on chromosome 4. Using sequence analysis, we identified the deletion of a 5-bp fragment and a transversion from G to A within LOC_Os04 g32510/LAX2, which caused early termination of translation in the afs1 mutant. These findings suggest that AFS1 may be a new allele of LAX2, and is involved in the development of floral organs by regulating the expression of genes related to their development. The above results provide a new view on the function of LAX2, which may also regulate the development of spikelets.
基金supported by the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality(IDHT20180509)the National Natural Science foundation of China(31201645)+1 种基金the Beijing Municipal Natural Science Foundation(6172006)key project of Beijing Municipal Education Commission(KZ201510020021)
文摘Syringa species are important ornamentals with strong floral scent,of which monoterpenes are the main component.In this study,a new monoterpene synthase gene,named SoLIM,was collected from the flowers of Syringa oblata and S.oblata var.alba using a homologous cloning method.The full-length cDNA of SoLIM was1746 bp and encoded 581 amino acids.Sequence analysis showed that SoLIM contained the DDxxD and RRx8 W motifs,which are two typical conserved monoterpene synthase motifs,and was thus classified as belonging to the Tpsb subfamily.Using quantitative reverse-transcription PCR,SoLIM was significantly expressed in the petals and pistils of S.oblata and S.oblata var.alba,respectively.SoLIM expression peaked earlier than the D-limonene emissions in the diurnal experiments,but occurred later when D-limonene had peaked during the flowering phase,indicating that differences in SoLIM gene expression and D-limonene emissions existed.The synthesis of floral scent is thus associated with diverse regulatory mechanisms that require further investigation.
文摘植物FLOWERING LOCUS T/TERMINAL FLOWER1(FT/TFL1)基因家族是一个进化上高度保守的基因家族,它在植物的花发育过程中具有重要作用:其成员FT基因编码的蛋白产物是可以长距离转运的成花激素,在花形成过程中起关键作用;另一成员TFL1基因则在花序的形成和维持过程中起重要作用.本文就近年来国内外对植物FT/TFL1基因家族的结构、成员,以及各个成员在花发育转换过程中的功能等研究现状进行综述,并对该基因家族的研究前景提出展望.
