Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic call...Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic callus, which was used to establish cell suspension lines. Cell suspensions had to be subcultured at a interval of 4-5 days at the first several culture cycles. The best subculturing cycle for the stable cell suspensions was 8-10 days. The best inoculum quantity was 1 mL PCV（Packed Cell Volume） per 40 mL culture fluid.展开更多
[Objective] This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas. [Method] A total of 8 floribunda rose cul- tivars （Carefree Wonder, Scloss Mannheim, Bright Smile,...[Objective] This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas. [Method] A total of 8 floribunda rose cul- tivars （Carefree Wonder, Scloss Mannheim, Bright Smile, Goldmarie, Betty Prior, Red Riding Hood Fairy Tale, Golden Scepter and Iceberg） were selected as the research objects, and their growth, adaptability and disease resistance in Sanya area were compared. [Result] Carefree Wonder, Scloss Mannheim, Iceberg and Bright Smile were suitable to be planted in Sanya area. Among them, Carefree Wonder, Scloss Mannheim and Iceberg were characterized with fewer diseases, bright colors, heat resistance and strong stress resistance, and they could be planted as ornamental flowers in Sanya area. [Conclusion] This study will provide a reference for the selection of ornamental flowers in Sanya area.展开更多
文摘Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic callus, which was used to establish cell suspension lines. Cell suspensions had to be subcultured at a interval of 4-5 days at the first several culture cycles. The best subculturing cycle for the stable cell suspensions was 8-10 days. The best inoculum quantity was 1 mL PCV（Packed Cell Volume） per 40 mL culture fluid.
基金Supported by Special Fund for Applied Technology Research and Development and Demonstration and Promotion in Hainan Province(ZDXM2014002)~~
文摘[Objective] This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas. [Method] A total of 8 floribunda rose cul- tivars （Carefree Wonder, Scloss Mannheim, Bright Smile, Goldmarie, Betty Prior, Red Riding Hood Fairy Tale, Golden Scepter and Iceberg） were selected as the research objects, and their growth, adaptability and disease resistance in Sanya area were compared. [Result] Carefree Wonder, Scloss Mannheim, Iceberg and Bright Smile were suitable to be planted in Sanya area. Among them, Carefree Wonder, Scloss Mannheim and Iceberg were characterized with fewer diseases, bright colors, heat resistance and strong stress resistance, and they could be planted as ornamental flowers in Sanya area. [Conclusion] This study will provide a reference for the selection of ornamental flowers in Sanya area.
