The study was carried out on the basis of the flowering period of mung bean (Phaseolus radiatus). The polyethylene glycol 6000 (PEG6000) was used to stimulate water stress. Four mung bean varieties with different drou...The study was carried out on the basis of the flowering period of mung bean (Phaseolus radiatus). The polyethylene glycol 6000 (PEG6000) was used to stimulate water stress. Four mung bean varieties with different drought resistances were used as materials in this experiment, in which the physiological indexes of mung bean for the identification of drought resistance were selected, and the response analysis of seven physiological indexes to different degrees of drought stress was measured by using polyethylene glycol 6000 (PEG6000) on different concentrations of 5%, 10% and 20%. The physiological mechanism was analysed on drought tolerance of mung beans. This test was to set up identification system for drought tolerance of flowering mung bean. The results showed that the relative conductivity after 6 days of 5% PEG treatment, the SOD activity after 6 days of 5% PEG treatment, the POD activity after 6 days of 10% PEG treatment and the ABA content after 6 days of 10% PEG treatment could be used as drought-tolerant identification for mung beans at flowering period. These four physiological indexes and drought-resistant index weighted grey correlation analysis results showed that the correlation order was successively SOD activity (0.8589) > ABA content (0.8290) > conductivity (0.7160) > POD activity (0.6637);and the grey correlation degree was greater than 0.6500 among the four physiological indexes.展开更多
In pot experiments of Xichang,China,during 1989-1994,visual scnescence symptoms and associated changes in constituent contents and activities of leaves of faba bean(Vicia faba L.)were compared in respones to flower re...In pot experiments of Xichang,China,during 1989-1994,visual scnescence symptoms and associated changes in constituent contents and activities of leaves of faba bean(Vicia faba L.)were compared in respones to flower removal.the leaves from upper, middle and lower positions were sampled six times during reproductive development phase.At 70 DAP flower removal had caused 37%-189% and 82%-197% increase of green leaf area and green leaf dry weight per plant respectively.Flower removal led to a significant increase in the chlorophyll,soluble surar and protein contents and the catalase activity.The leaf cell relative electroconductivity of those plants was maintained at a lower level,relative to the control,during the late growing stage.These results certainly implied that the leaves of flower removal plants were still fully functional at a very late growing stage,consequently the plants increased many new branches per plant.展开更多
In pot experiments at Xichang,China,during 1989~1994,visual senescencesymptoms and associated changes in constituent contents and activities of leaves of fababean(Vicia faba L.)were compared in respones to flower rem...In pot experiments at Xichang,China,during 1989~1994,visual senescencesymptoms and associated changes in constituent contents and activities of leaves of fababean(Vicia faba L.)were compared in respones to flower removal.The leaves from upper,middle and lower positions were sampled six times during reproductive developmentphase.At 70 DAP flower removal had caused 37%~189% and 82%~197% increase ofgreen leaf area and green leaf dry weight per plant respectively.Flower removal led to asignificant increase in the chlorophyll,soluble sugar and protein contents and the catalaseactivity.The leaf cell relative electroconductivity of those plants was maintained at a lowerlevel,relative to the control,during the late growing stage.These results certainly impliedthat the leaves of flower removal plants were still fully functional at a very late growingstage,consequently the plants increased many new branches per plant.展开更多
为了提高蚕豆花左旋多巴的提取得率,采用加热回流提取法优化蚕豆花左旋多巴提取工艺。通过单因素和正交试验设计,考察液料比、溶剂p H、提取时间对蚕豆花左旋多巴提取得率的影响,确定最佳提取工艺条件为:液料比20:1,溶剂p H 4.5,提取时...为了提高蚕豆花左旋多巴的提取得率,采用加热回流提取法优化蚕豆花左旋多巴提取工艺。通过单因素和正交试验设计,考察液料比、溶剂p H、提取时间对蚕豆花左旋多巴提取得率的影响,确定最佳提取工艺条件为:液料比20:1,溶剂p H 4.5,提取时间2 h,在此条件下左旋多巴提取得率为3.67%,相比冷浸法提高了3.67%。本工艺中使用乙醇来调节溶剂的p H值,可以抑制水溶性杂质的析出,经HPLC谱图分析,提取物中杂质组分较少,且左旋多巴与杂质组分分离较好,这有利于左旋多巴进一步的纯化。本研究可为从蚕豆花中工业化提取左旋多巴提供技术依据。展开更多
文摘The study was carried out on the basis of the flowering period of mung bean (Phaseolus radiatus). The polyethylene glycol 6000 (PEG6000) was used to stimulate water stress. Four mung bean varieties with different drought resistances were used as materials in this experiment, in which the physiological indexes of mung bean for the identification of drought resistance were selected, and the response analysis of seven physiological indexes to different degrees of drought stress was measured by using polyethylene glycol 6000 (PEG6000) on different concentrations of 5%, 10% and 20%. The physiological mechanism was analysed on drought tolerance of mung beans. This test was to set up identification system for drought tolerance of flowering mung bean. The results showed that the relative conductivity after 6 days of 5% PEG treatment, the SOD activity after 6 days of 5% PEG treatment, the POD activity after 6 days of 10% PEG treatment and the ABA content after 6 days of 10% PEG treatment could be used as drought-tolerant identification for mung beans at flowering period. These four physiological indexes and drought-resistant index weighted grey correlation analysis results showed that the correlation order was successively SOD activity (0.8589) > ABA content (0.8290) > conductivity (0.7160) > POD activity (0.6637);and the grey correlation degree was greater than 0.6500 among the four physiological indexes.
文摘In pot experiments of Xichang,China,during 1989-1994,visual scnescence symptoms and associated changes in constituent contents and activities of leaves of faba bean(Vicia faba L.)were compared in respones to flower removal.the leaves from upper, middle and lower positions were sampled six times during reproductive development phase.At 70 DAP flower removal had caused 37%-189% and 82%-197% increase of green leaf area and green leaf dry weight per plant respectively.Flower removal led to a significant increase in the chlorophyll,soluble surar and protein contents and the catalase activity.The leaf cell relative electroconductivity of those plants was maintained at a lower level,relative to the control,during the late growing stage.These results certainly implied that the leaves of flower removal plants were still fully functional at a very late growing stage,consequently the plants increased many new branches per plant.
文摘In pot experiments at Xichang,China,during 1989~1994,visual senescencesymptoms and associated changes in constituent contents and activities of leaves of fababean(Vicia faba L.)were compared in respones to flower removal.The leaves from upper,middle and lower positions were sampled six times during reproductive developmentphase.At 70 DAP flower removal had caused 37%~189% and 82%~197% increase ofgreen leaf area and green leaf dry weight per plant respectively.Flower removal led to asignificant increase in the chlorophyll,soluble sugar and protein contents and the catalaseactivity.The leaf cell relative electroconductivity of those plants was maintained at a lowerlevel,relative to the control,during the late growing stage.These results certainly impliedthat the leaves of flower removal plants were still fully functional at a very late growingstage,consequently the plants increased many new branches per plant.