Objective:To investigate the effect of two extracts of Bougainvillea spectabilis(B. spectabilis) flowers with yellow and pink/purple on brain oxidative stress and neuronal damage caused in rats by systemic rotenone in...Objective:To investigate the effect of two extracts of Bougainvillea spectabilis(B. spectabilis) flowers with yellow and pink/purple on brain oxidative stress and neuronal damage caused in rats by systemic rotenone injection. Methods:Rotenone 1.5 mg/kg was given three times per week alone or in combination with B. spectabilis flowers extracts(25 mg or 50 mg) via the subcutaneous route for 2 weeks. Brain concentrations of the lipid peroxidation marker malondialdehyde(MDA),reduced glutathione,nitric oxide(nitrite),the pro-inflammatory cytokine interleukin-1beta(Il-1β) as well as butyrylcholinesterase,and paraoxonase-1(PON-1) activities,were determined. Histopathology and caspase-3 immunohistochemistry were also performed. Results:Rotenone resulted in significant increases of brain MDA(the product of lipid peroxidation),and nitric oxide content along with decreased brain reduced glutathione. There were also marked and significant inhibition of brain PON-1 and BCh E activities and increased Il-1β in brain of rotenone-treated rats. B. spectabilis flowers extract itself resulted in brain oxidative stress increasing both lipid peroxidation and nitrite content whilst inhibiting PON-1 activity. The yellow flowers extract inhibited BCh E activity and increased brain Il-1β. When given to rotenone-treated rats,B. spectabilis extracts,however,decreased lipid peroxidation while their low administered doses increased brain GSH. Brain nitrite decreased by the pink extract but showed further increase by the yellow extract. Either extract,however,caused further inhibition of PON-1 activity while the yellow extract resulted in further inhibition of BChE activity. Histopathological studies indicated that both extracts protected against brain,liver and kidney damage caused by the toxicant. Conclusions:These data indicate that B. spectabilis flowers extracts exert protective effect against the toxic effects of rotenone on brain,liver and kidney. B. spectabilis flowers extracts decreased brain lipid peroxidation and prevented neuronal death due to rotenone and might thus prove the value in treatment of Parkinson's disease.展开更多
用三唑酮(6.25%)+乙蒜素(93.75%)混合配制成160 m g/L至1 600 m g/L不同浓度的药剂(10种),对九重葛插穗浸泡处理3 h后扦插。实验结果,各种处理中最佳的插穗生根数、根长、根径的平均值分别为23.2根、20.5 mm、1.1 mm,最高成活率达到86....用三唑酮(6.25%)+乙蒜素(93.75%)混合配制成160 m g/L至1 600 m g/L不同浓度的药剂(10种),对九重葛插穗浸泡处理3 h后扦插。实验结果,各种处理中最佳的插穗生根数、根长、根径的平均值分别为23.2根、20.5 mm、1.1 mm,最高成活率达到86.7%,所测生长指标与对照组的差异极显著(P<0.01)。在160 m g/L至400 m g/L范围,药剂的处理浓度与插穗的生根数、根长、根径以及成活率的关系均符合二次曲线模型(R2>0.98),而与枝叶的生长呈负相关(R2>0.95),但与对照的差异不显著。统计分析结果,三唑酮.乙蒜素混剂对九重葛插穗的最佳处理剂量为266 m g/L。展开更多
文摘Objective:To investigate the effect of two extracts of Bougainvillea spectabilis(B. spectabilis) flowers with yellow and pink/purple on brain oxidative stress and neuronal damage caused in rats by systemic rotenone injection. Methods:Rotenone 1.5 mg/kg was given three times per week alone or in combination with B. spectabilis flowers extracts(25 mg or 50 mg) via the subcutaneous route for 2 weeks. Brain concentrations of the lipid peroxidation marker malondialdehyde(MDA),reduced glutathione,nitric oxide(nitrite),the pro-inflammatory cytokine interleukin-1beta(Il-1β) as well as butyrylcholinesterase,and paraoxonase-1(PON-1) activities,were determined. Histopathology and caspase-3 immunohistochemistry were also performed. Results:Rotenone resulted in significant increases of brain MDA(the product of lipid peroxidation),and nitric oxide content along with decreased brain reduced glutathione. There were also marked and significant inhibition of brain PON-1 and BCh E activities and increased Il-1β in brain of rotenone-treated rats. B. spectabilis flowers extract itself resulted in brain oxidative stress increasing both lipid peroxidation and nitrite content whilst inhibiting PON-1 activity. The yellow flowers extract inhibited BCh E activity and increased brain Il-1β. When given to rotenone-treated rats,B. spectabilis extracts,however,decreased lipid peroxidation while their low administered doses increased brain GSH. Brain nitrite decreased by the pink extract but showed further increase by the yellow extract. Either extract,however,caused further inhibition of PON-1 activity while the yellow extract resulted in further inhibition of BChE activity. Histopathological studies indicated that both extracts protected against brain,liver and kidney damage caused by the toxicant. Conclusions:These data indicate that B. spectabilis flowers extracts exert protective effect against the toxic effects of rotenone on brain,liver and kidney. B. spectabilis flowers extracts decreased brain lipid peroxidation and prevented neuronal death due to rotenone and might thus prove the value in treatment of Parkinson's disease.
文摘用三唑酮(6.25%)+乙蒜素(93.75%)混合配制成160 m g/L至1 600 m g/L不同浓度的药剂(10种),对九重葛插穗浸泡处理3 h后扦插。实验结果,各种处理中最佳的插穗生根数、根长、根径的平均值分别为23.2根、20.5 mm、1.1 mm,最高成活率达到86.7%,所测生长指标与对照组的差异极显著(P<0.01)。在160 m g/L至400 m g/L范围,药剂的处理浓度与插穗的生根数、根长、根径以及成活率的关系均符合二次曲线模型(R2>0.98),而与枝叶的生长呈负相关(R2>0.95),但与对照的差异不显著。统计分析结果,三唑酮.乙蒜素混剂对九重葛插穗的最佳处理剂量为266 m g/L。
