The simple approach to acquire the fltaxescenee spectra of vitamin bl, b2 and t6 is proposed by direct ilhanination on solid powder sample. The experimentally acquired fluorescence spectra are in accordance with the p...The simple approach to acquire the fltaxescenee spectra of vitamin bl, b2 and t6 is proposed by direct ilhanination on solid powder sample. The experimentally acquired fluorescence spectra are in accordance with the previous measurements on soluble samples. The fluorescence spectra for a mixture of vitamins bl, b2 and b6 with different concentrations have been investigated, and the fluorescence mechanism is explained on the basis of moleoalar struchture Possible reasons of the blue-shift of the fluorescence peak and enhancement of the peak power are explained as well. The advantages of solid powder method is analyzed and discussed.展开更多
As is well known, the fluorescence spectra of amino-acids can be varied greatly by pH. However, up to date, the effect of organic or inorganic acid on the fluorescence spectra of non-ionic organic compounds is not cle...As is well known, the fluorescence spectra of amino-acids can be varied greatly by pH. However, up to date, the effect of organic or inorganic acid on the fluorescence spectra of non-ionic organic compounds is not clear, although much attention has been paid to this subject in the present decadee. In 1969, Smith reported that the fluorescence of methoxybenzene can be quenched by acid. He has展开更多
Since F?rster discovered the fluorescence spectrum of excimer in 1954, a lot of work has been done in this field. It is generally considered that there is no preformed excimer site in the ground state because the abso...Since F?rster discovered the fluorescence spectrum of excimer in 1954, a lot of work has been done in this field. It is generally considered that there is no preformed excimer site in the ground state because the absorption spectrum is unchanged. Excimer is formed only when the molecules have traveled a certain distance by molecular motion and attained a suitable orientation by diffusion-controlled process in the excited state. But Ferguson and Avis et al. discovered the excimer fluorescence展开更多
The accumulation courses of 11S and 7S proteins in the development seed of soybean were studied by means of immuno-fluorescence. Fifteen days after flowering, 7S proteins began to deposit and so did 11S proteins a lit...The accumulation courses of 11S and 7S proteins in the development seed of soybean were studied by means of immuno-fluorescence. Fifteen days after flowering, 7S proteins began to deposit and so did 11S proteins a little later. It was observed that 20—30 days after flowering, these two globulins or their precursor peptides had the tendency to transfer from tegument to the cotyledon. It was found that a few of the cotyledon cells in the mature seed did not accumulate 11S proteins, while in the high protein cultivar and high protein wild soybean all the cotyledon cells were filled with 11S proteins and other storage substances.展开更多
Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dy...Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dynamics.Herein,inspired by the cellular machinery of reversible thiol modification by NO,we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics.展开更多
Polymers containing chromophores on the side chain like polystyrene, polyvinylnaphthalene or in the main chain like polyethylene-2,6 naphthalenedicarboxylate (PEN) show both monomer and exeimer fluorescence in solutio...Polymers containing chromophores on the side chain like polystyrene, polyvinylnaphthalene or in the main chain like polyethylene-2,6 naphthalenedicarboxylate (PEN) show both monomer and exeimer fluorescence in solutions, but only the excimer fluorescence in solid films. This fact remains not unambiguously explained.展开更多
基金supported by the Tianjin Natural Science Foundation(No.06YFJZJC00300)the Natural Science Foundation of China(Nos.10674075,10774077,10674074,50802044 and 60736039)
文摘The simple approach to acquire the fltaxescenee spectra of vitamin bl, b2 and t6 is proposed by direct ilhanination on solid powder sample. The experimentally acquired fluorescence spectra are in accordance with the previous measurements on soluble samples. The fluorescence spectra for a mixture of vitamins bl, b2 and b6 with different concentrations have been investigated, and the fluorescence mechanism is explained on the basis of moleoalar struchture Possible reasons of the blue-shift of the fluorescence peak and enhancement of the peak power are explained as well. The advantages of solid powder method is analyzed and discussed.
文摘As is well known, the fluorescence spectra of amino-acids can be varied greatly by pH. However, up to date, the effect of organic or inorganic acid on the fluorescence spectra of non-ionic organic compounds is not clear, although much attention has been paid to this subject in the present decadee. In 1969, Smith reported that the fluorescence of methoxybenzene can be quenched by acid. He has
文摘Since F?rster discovered the fluorescence spectrum of excimer in 1954, a lot of work has been done in this field. It is generally considered that there is no preformed excimer site in the ground state because the absorption spectrum is unchanged. Excimer is formed only when the molecules have traveled a certain distance by molecular motion and attained a suitable orientation by diffusion-controlled process in the excited state. But Ferguson and Avis et al. discovered the excimer fluorescence
文摘The accumulation courses of 11S and 7S proteins in the development seed of soybean were studied by means of immuno-fluorescence. Fifteen days after flowering, 7S proteins began to deposit and so did 11S proteins a little later. It was observed that 20—30 days after flowering, these two globulins or their precursor peptides had the tendency to transfer from tegument to the cotyledon. It was found that a few of the cotyledon cells in the mature seed did not accumulate 11S proteins, while in the high protein cultivar and high protein wild soybean all the cotyledon cells were filled with 11S proteins and other storage substances.
基金supported by the National Natural Science Foundations of China(nos.22077112,21778048,81673489,31871414,and 81125023)Natural Science Foundation of Zhejiang Province,China(no.LR18H300001)National Science and Technology Major Project“Key New Drug Creation and Manufacturing Program”(nos.2018ZX09711002-010-004,2018ZX09711002-007-002,2019ZX09201001-003-009,2019ZX09201001-003-010,and 2019ZX09201001-004-010),K.C.Wong Education Foundation,and Singapore University of Technology and Design(SUTD)[SUTD-ZJU IDEA grant nos.T1SRCI17126 and SUTD-ZJU(VP)201905].
文摘Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dynamics.Herein,inspired by the cellular machinery of reversible thiol modification by NO,we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics.
文摘Polymers containing chromophores on the side chain like polystyrene, polyvinylnaphthalene or in the main chain like polyethylene-2,6 naphthalenedicarboxylate (PEN) show both monomer and exeimer fluorescence in solutions, but only the excimer fluorescence in solid films. This fact remains not unambiguously explained.
