Expression differences of flavonoid synthase I（BmFNS I）gene in the midgut,silk gland and fat body of the male and female Bombyx Mori with sex-identified fluorescent cocoon color were detected by fluorescence quantit...Expression differences of flavonoid synthase I（BmFNS I）gene in the midgut,silk gland and fat body of the male and female Bombyx Mori with sex-identified fluorescent cocoon color were detected by fluorescence quantitative PCR.The result showed that the relative expression level of BmFNS I gene in midgut of Bombyx Mori was the highest,followed by that in fat body,and the expression level in silk gland was the lowest,indicating that BmFNS I gene played an important role in flavonoids anabolism in mulberry leaves,which was the food of silkworm.However,there was no significant difference in BmFNS I gene expression level between midguts of male and female Bombyx Mori species with sex-identified fluorescent cocoon color,suggesting that there was no difference in flavonoids anabolism of mulberry leaves between the male and female Bombyx Mori with sex-identified fluorescent cocoon color,at least no difference in flavonoids anabolism regulated by FSN I gene.展开更多
To study the clinical and cytogenetic characteristics of four patients with myel odysplastic syndrome (MDS) and one with acute myeloid leukemia experiencing t(1; 7) Methods Five patients seen in our hospital from 19...To study the clinical and cytogenetic characteristics of four patients with myel odysplastic syndrome (MDS) and one with acute myeloid leukemia experiencing t(1; 7) Methods Five patients seen in our hospital from 1992 to 2001 were diagnosed as MDS and acute myelocytic leukemia (AML) according to the French American British (FAB) criteria Chromosomes were prepared using the direct method as well as 24 hou r unstimulated cultures of fresh heparinized bone marrow for each subject, while R banding was used to analyze karyotypes Dual color fluorescence in situ hy bridization (FISH) using SpectrumRed and SpectrumGreen directly labeled chromoso me 1 specific α satellite DNA probe (red) and chromosome 7 specific α sat ellite DNA probe (green) was performed for three cases Results Of the five patients, three had 1;7 translocation due to a long history of expos ure to benzene In three cases, dual color FISH resulted in three red signals and two green ones, in which one red signal adjoining one green signal in 27 6% , 84% and 18 5% metaphases, respectively Conclusions Exposure to benzene may be the cause for Chinese MDS and AML patients with t(1;7 ) translocation The result of dual color FISH convincingly confirmed that the centromere of the derivative chromosome 7p/1q resulting from 1;7 translocation was made up of centromeres from both chromosomes 1 and 7展开更多
基金Supported by the Major State Basic Research Development Program of China(863Program)(2006AA10A118)Natural Science Foundation of the Higher Education Institutions of Jiangsu Province,China(07KJB230102)~~
文摘Expression differences of flavonoid synthase I（BmFNS I）gene in the midgut,silk gland and fat body of the male and female Bombyx Mori with sex-identified fluorescent cocoon color were detected by fluorescence quantitative PCR.The result showed that the relative expression level of BmFNS I gene in midgut of Bombyx Mori was the highest,followed by that in fat body,and the expression level in silk gland was the lowest,indicating that BmFNS I gene played an important role in flavonoids anabolism in mulberry leaves,which was the food of silkworm.However,there was no significant difference in BmFNS I gene expression level between midguts of male and female Bombyx Mori species with sex-identified fluorescent cocoon color,suggesting that there was no difference in flavonoids anabolism of mulberry leaves between the male and female Bombyx Mori with sex-identified fluorescent cocoon color,at least no difference in flavonoids anabolism regulated by FSN I gene.
文摘To study the clinical and cytogenetic characteristics of four patients with myel odysplastic syndrome (MDS) and one with acute myeloid leukemia experiencing t(1; 7) Methods Five patients seen in our hospital from 1992 to 2001 were diagnosed as MDS and acute myelocytic leukemia (AML) according to the French American British (FAB) criteria Chromosomes were prepared using the direct method as well as 24 hou r unstimulated cultures of fresh heparinized bone marrow for each subject, while R banding was used to analyze karyotypes Dual color fluorescence in situ hy bridization (FISH) using SpectrumRed and SpectrumGreen directly labeled chromoso me 1 specific α satellite DNA probe (red) and chromosome 7 specific α sat ellite DNA probe (green) was performed for three cases Results Of the five patients, three had 1;7 translocation due to a long history of expos ure to benzene In three cases, dual color FISH resulted in three red signals and two green ones, in which one red signal adjoining one green signal in 27 6% , 84% and 18 5% metaphases, respectively Conclusions Exposure to benzene may be the cause for Chinese MDS and AML patients with t(1;7 ) translocation The result of dual color FISH convincingly confirmed that the centromere of the derivative chromosome 7p/1q resulting from 1;7 translocation was made up of centromeres from both chromosomes 1 and 7
