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Spectral selective fluorescence molecular imaging with volume holographic imaging system
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作者 Yanlu Lv Jiulou Zhang +4 位作者 Fei Liu Junwei Shi Huizhi Guang Jing Bai Jianwen Luo 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2016年第2期59-69,共11页
A compact volume holographic imaging(VHI)method that can detect fluorescence objects located in diffusive medium in spectral selective imaging manner is presented.The enlargement of lateralfield of view of the VHI sys... A compact volume holographic imaging(VHI)method that can detect fluorescence objects located in diffusive medium in spectral selective imaging manner is presented.The enlargement of lateralfield of view of the VHI system is realized by using broadband illumination and demagnification optics.Each target spectrum of°uorescence emitting from a di®usive medium is probed by tuning the inclination angle of the transmission volume holographic grating(VHG).With the use of the single transmission VHG,fluorescence images with different spectrum are obtained sequentially and precise three-dimensional(3D)information of deep fluorescent objects located in a diffusive medium can be reconstructed from these images.The results of phantom experiments demonstrate that two fluorescent objects with a sub-millimeter distance can be resolved by spectral selective imaging. 展开更多
关键词 Volume holographic grating diffusive medium fluorescence molecular imaging
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DEVELOPMENT OF NEEDLE-BASED MICROENDOSCOPY FOR FLUORESCENCE MOLECULAR IMAGING OF BREAST TUMOR MODELS 被引量:1
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作者 CHAO-WEI CHEN TIFFANY R.BLACKWELL +4 位作者 RENEE NAPHAS PAUL T.WINNARD JR VENU RAMAN KRISTINE GLUNDE YU CHEN 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2009年第4期343-352,共10页
Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression,enzyme activity,and disease-specific molecular interactions in vivo using targeted contrast agents,and ther... Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression,enzyme activity,and disease-specific molecular interactions in vivo using targeted contrast agents,and therefore,is being developed for early detection and in situ characterization of breast cancers.Recent advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model systems.In synergy with contrast agent development,this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy procedures.This microendoscopy probe is based on a gradient-index(GRIN)lens interfaced with a laser scanning microscope.Specifications of the imaging performance,including the field-of-view,transverse resolution,and focus tracking characteristics were calibrated.Orthotopic MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein(RFP)were used to detect the tumor cells in this tumor model as a proof of principle study.With further development,this technology,in conjunction with the development of clinically applicable,injectable fluorescent molecular imaging agents,promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance. 展开更多
关键词 Breast cancer fluorescence molecular imaging MICROENDOSCOPY optical imaging tdTomato fluorescent protein.
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MINIMIZING EXCITATION LIGHT LEAKAGE AND MAXIMIZING MEASUREMENT SENSITIVITY FOR MOLECULAR IMAGING WITH NEAR-INFRARED FLUORESCENCE
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作者 BANGHE ZHU EVA M.SEVICK-MURACA 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2011年第3期301-307,共7页
Near-infraredfluorescence(NIRF)imaging involves the separation of weakfluorescence signals from backscattered excitation light.The measurement sensitivity of current NIRF imaging systems is limited by the excitation l... Near-infraredfluorescence(NIRF)imaging involves the separation of weakfluorescence signals from backscattered excitation light.The measurement sensitivity of current NIRF imaging systems is limited by the excitation light leakage through rejectionfilters.In this contribution,the authors demonstrate that the excitation light leakage can be suppressed upon using appropriatefilter combination and permutations.The excitation light leakage and measurement sensitivity were assessed and compared in this study by computing the transmission ratios of excitation to emission light collected and the signal-to-noise ratios in well-controlled phantom studies with differentfilter combinations and permutations.Using appropriatefilter combinations and permutations,we observe as much as two orders of magnitude reduction in the transmission ratio and higher signal-to-noise ratio. 展开更多
关键词 molecular imaging fluorescence opticalfilter excitation light leakage noise floor
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Fast in vivo bioluminescence tomography using a novel pure optical imaging technique
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作者 Shuang Zhang Chengcai Leng +2 位作者 Hongbo Liu Kun Wang Jie Tian 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2017年第3期77-88,共12页
Bioluminescence tomography(BLT)is a novel opt ical molecular imaging technique that advanced the conventional planar bioluminescence imaging(BLI)into a quantifiable three-dimensional(3D)approach in preclinical living ... Bioluminescence tomography(BLT)is a novel opt ical molecular imaging technique that advanced the conventional planar bioluminescence imaging(BLI)into a quantifiable three-dimensional(3D)approach in preclinical living animal studies in oncology.In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately,the prior structural information is com-monly obtained from X ray computed tomography(CT).This strategy requires a complicated hybrid imaging system,extensive post imaging analysis and involvement of ionizing radiation.Moreover,the overall robustness highly depends on the fusion accuracy between the optical and structural information.Here,we present a pure optical bioluminescence tomographic(POBT)system and a novel BLT workfow based on multi-view projection acquisition and 3D surface reconstruction.This met hod can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images,so that the prior structural information can be offered for 3D tumor lesion reconstruction without the involvement of CT.The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomo-graphic(DMT)system and a commercialized optical imaging system(IVIS Spectrum)using three breast cancer xenografts.The results revealed that the new technique offered comparable in vivo tomographic accuracy with the DMT system(P>0.05)in much shorter data analysis time.It also offered significantly better accuracy comparing with the IVIS system(P<0.04)without sacrificing too much time. 展开更多
关键词 Optical surface reconstruction bioluminescence tomography reconstruction optical molecular imaging light Aux reconstruction.
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Tikhonov-regularization-based projecting sparsity pursuit method for fluorescence molecular tomography reconstruction 被引量:2
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作者 Jiaju Cheng Jianwen Luo 《Chinese Optics Letters》 SCIE EI CAS CSCD 2020年第1期64-69,共6页
For fluorescence molecular tomography(FMT),image quality could be improved by incorporating a sparsity constraint.The L1 norm regularization method has been proven better than the L2 norm,like Tikhonov regularization.... For fluorescence molecular tomography(FMT),image quality could be improved by incorporating a sparsity constraint.The L1 norm regularization method has been proven better than the L2 norm,like Tikhonov regularization.However,the Tikhonov method was found capable of achieving a similar quality at a high iteration cost by adopting a zeroing strategy.By studying the reason,a Tikhonov-regularization-based projecting sparsity pursuit method was proposed that reduces the iterations significantly and achieves good image quality.It was proved in phantom experiments through time-domain FMT that the method could obtain higher accuracy and less oversparsity and is more applicable for heterogeneous-target reconstruction,compared with several regularization methods implemented in this Letter. 展开更多
关键词 fluorescence molecular TOMOGRAPHY SPARSITY PURSUIT Tikhonov REGULARIZATION GOOD image quality high efficiency
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IMAGING RNA IN LIVING CELLS WITH MOLECULAR BEACONS:CURRENT PERSPECTIVES AND CHALLENGES 被引量:1
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作者 ANTONY K.CHEN ANDREW TSOURKAS 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2009年第4期315-324,共10页
There is a growing realization that cell-to-cell variations in gene expression have importantbiological consequences underlying phenotype diversity and cell fate. Although analytical toolsfor measuring gene expression... There is a growing realization that cell-to-cell variations in gene expression have importantbiological consequences underlying phenotype diversity and cell fate. Although analytical toolsfor measuring gene expression, such as DNA microarrays, reverse-transcriptase PCR and in situhybridization have been widely utilized to discover the role of genetic variations in governingcellular behavior, these methods are performed in cell lysates and/or on fixed cells, and thereforelack the ability to provide comprehensive spatial-dynamic information on gene expression. Thishas invoked the recent development of molecular imaging strategies capable of illuminatingthe distribution and dynamics of RNA molecules in living cells. In this review, we describe aclass of molecular imaging probes known as molecular beacons (MBs), which have increasinglybecome the probe of choice for imaging RNA in living cells. In addition, we present the majorchallenges that can limit the ability of MBs to provide accurate measurements of RNA, anddiscuss efforts that have been made to overcome these challenges. It is envisioned that withcontinued refinement of the MB design, MBs will eventually become an indispensable tool foranalyzing gene expression in biology and medicine. 展开更多
关键词 Gene expression fluorescence probes molecular imaging BIOSENSOR
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Recent advances in ultrasound-controlled fluorescence technology for deep tissue optical imaging
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作者 Rui-Lin Liu Ru-Qian Cai 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2022年第4期530-540,共11页
Fluorescence imaging is a noninvasive and dynamic real-time imaging technique;however,it exhibits poor spatial resolution in centimeter-deep tissues because biological tissues are highly scattering media for optical r... Fluorescence imaging is a noninvasive and dynamic real-time imaging technique;however,it exhibits poor spatial resolution in centimeter-deep tissues because biological tissues are highly scattering media for optical radiation.The recently developed ultrasound-controlled fluorescence(UCF)imaging is a novel imaging technique that can overcome this bottleneck.Previous studies suggest that the effective contrast agent and sensitive imaging system are the two pivotal factors for generating high-resolution UCF images ex vivo and/or in vivo.Here,this review highlights the recent advances(2015e2020)in the design and synthesis of contrast agents and the improvement of imaging systems to realize high-resolution UCF imaging of deep tissues.The imaging performances of various UCF systems,including the signal-to-noise ratio,imaging resolution,and imaging depth,are specifically discussed.In addition,the challenges and prospects are highlighted.With continuously increasing research interest in this field and emerging multidisciplinary applications,UCF imaging with higher spatial resolution and larger imaging depth may be developed shortly,which is expected to have a far-reaching impact on disease surveillance and/or therapy. 展开更多
关键词 Ultrasound-controlled fluorescence imaging Temperature-sensitive NIR probes High-resolution Deep tissue molecular diagnosis
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Minimally Invaded Sentinel Lymph Node Model for the Development of Intraoperative Infrared Fluorescence Imaging
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作者 Badiane Serigne Moussa Raes Florian +3 位作者 Julien Sobilo Natkunarajah Sharuja Lerondel Stéphanie Le Pape Alain 《Advances in Molecular Imaging》 2018年第4期48-58,共11页
In this study we implemented an axillary SLN invasion model to develop highly sensitive imaging strategies enabling detection of a very small amount of tumor cells. A highly diffusible molecular probe targeting &a... In this study we implemented an axillary SLN invasion model to develop highly sensitive imaging strategies enabling detection of a very small amount of tumor cells. A highly diffusible molecular probe targeting &alpha;v&beta;3 and &alpha;v&beta;5 integrins was investigated either via IV or locoregional injections. We additionally documented the potential interferences of this Near Infrared Fluorescence Probe with Blue Patente V and ICG dyes routinely used to facilitate lymph node detection during surgery. The human mammary adenocarcinoma MDA-MB-231-luc model was injected into the forepaw of nude female rats to obtain a controlled invasion of the axillary LN. Thanks to its high sensitivity, BLI was selected to achieve in vivo quantitation of tumor cells in SLNs and determine eligible animals for the study. NIRF of integrins was performed at 680 nm both in vivo and ex vivo using spectral unmixing to suppress auto-fluorescence signal and preserve sensitivity. In vivo BLI was quite reliable in estimating discrete invasion by cancer cells in the LN with thresholds of detection and quantitation of about 500 and 1500 cells respectively. For fluorescence at 680 nm, in vivo imaging is not suitable to detect micro-invasion, but ex vivo fluorescence with spectral unmixing of SLNs confirmed the presence of a tumor burden as low as 1500 cells expressing &alpha;v&beta;3/&alpha;v&beta;5 integrins. Targeting few tumor cells inside a micro-invaded sentinel lymph node by molecular probes is not sensitive enough to provide direct in vivo or peroperative imaging. At the time NIRF is performed on the excised specimen, high sensitivity imaging associated with spectral unmixing allowed such detection within less than 1 minute of examination. 展开更多
关键词 Sentinel LYMPH Node Near INFRARED fluorescence imaging Photoacoustic imaging molecular imaging
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IN VIVO VALIDATION OF DUAL-MODALITY SYSTEM FOR SIMULTANEOUS POSITRON EMISSION TOMOGRAPHY AND OPTICAL TOMOGRAPHIC IMAGING
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作者 XIN WANG BIN ZHANG +4 位作者 XU CAO FEI LIU SHUANGQUAN LIU BAOCI SHAN JING BAI 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2011年第2期165-171,共7页
We report on tests of combined positron emission tomography(PET)andfluorescence molecular tomography(FMT)imaging system for in vivo investigation on small animals.A nude mouse was inoculated with MD-MB-231 breast canc... We report on tests of combined positron emission tomography(PET)andfluorescence molecular tomography(FMT)imaging system for in vivo investigation on small animals.A nude mouse was inoculated with MD-MB-231 breast cancer cells which expressed redfluorescent protein(RFP).For FMT system,reflective illumination mode was adopted with full-angle data acquisition.[18F]-Fluorodeoxyglucose([18F]-FDG)was used as radioactive tracer for PET.Both data were acquired simultaneously and then reconstructed separately before fusion.Fluorescent tomography results showed exactly where the tumor was located while PET results offered more metabolic information.Results confirmed feasibility for tumor detection and showed superiority to single modality imaging. 展开更多
关键词 Dual-modality imaging positron emission tomography fluorescence molecular tomography
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Recent methodology advances in fluorescence molecular tomography
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作者 Yu An Kun Wang Jie Tian 《Visual Computing for Industry,Biomedicine,and Art》 2018年第1期1-11,共11页
Molecular imaging(MI)is a novel imaging discipline that has been continuously developed in recent years.It combines biochemistry,multimodal imaging,biomathematics,bioinformatics,cell&molecular physiology,biophysic... Molecular imaging(MI)is a novel imaging discipline that has been continuously developed in recent years.It combines biochemistry,multimodal imaging,biomathematics,bioinformatics,cell&molecular physiology,biophysics,and pharmacology,and it provides a new technology platform for the early diagnosis and quantitative analysis of diseases,treatment monitoring and evaluation,and the development of comprehensive physiology.Fluorescence Molecular Tomography(FMT)is a type of optical imaging modality in MI that captures the three-dimensional distribution of fluorescence within a biological tissue generated by a specific molecule of fluorescent material within a biological tissue.Compared with other optical molecular imaging methods,FMT has the characteristics of high sensitivity,low cost,and safety and reliability.It has become the research frontier and research hotspot of optical molecular imaging technology.This paper took an overview of the recent methodology advances in FMT,mainly focused on the photon propagation model of FMT based on the radiative transfer equation(RTE),and the reconstruction problem solution consist of forward problem and inverse problem.We introduce the detailed technologies utilized in reconstruction of FMT.Finally,the challenges in FMT were discussed.This survey aims at summarizing current research hotspots in methodology of FMT,fromwhich future research may benefit. 展开更多
关键词 fluorescence molecular tomography Image reconstruction Photon propagation model Forward problem Inverse problem
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Latest advances of X-ray imaging and biomedical applications beamline at SSRF 被引量:10
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作者 谢红兰 邓彪 +10 位作者 杜国浩 付亚楠 陈荣昌 周光照 任玉琦 王玉丹 薛艳玲 彭冠云 和友 郭瀚 肖体乔 《Nuclear Science and Techniques》 SCIE CAS CSCD 2015年第2期6-21,共16页
On May 6, 2009, the X-ray imaging and biomedical application beamline(BL13W1) at Shanghai Synchrotron Radiation Facility(SSRF) officially opened to users, with 8–72.5 ke V X-rays. The experimental station is equipped... On May 6, 2009, the X-ray imaging and biomedical application beamline(BL13W1) at Shanghai Synchrotron Radiation Facility(SSRF) officially opened to users, with 8–72.5 ke V X-rays. The experimental station is equipped with four sets of X-ray CCD detectors of different pixel size(0.19–24 μm) for on-line phase-contrast imaging and micro-CT imaging with 0.8 μm spatial resolution and 1 ms temporal resolution. An in vivo microCT experiment for a living insect was realized in 4 s. An X-ray fluorescence detector is equipped for X-ray fluorescence mapping imaging and X-ray fluorescence micro-CT imaging with 50 μm spatial resolution. In order to meet different requirements from the users, several experimental methods, such as X-ray spiral micro-CT, Xray local micro-CT, X-ray fast micro-CT, X-ray grating-based differential micro-CT, X-ray fluorescence microCT and X-ray quantitative micro-CT have been developed, and nearly 60 papers related to those developments for this beamline have been published. Moreover, the beamline has realized the remote fast CT reconstruction,providing a great convenience for the users to process experimental data at their offices. As of August 2014,the beamline has offered the user beamtime of(23 145 h), from which 232 user papers have been published,including 151 SCI papers and 55 papers with SCI impact factor > 3. The quantity and quality of the user paper outcome keep a steady increase. Some typical user experimental results are introduced. 展开更多
关键词 X射线成像 生物医学应用 SSRF 光束线 上海同步辐射装置 空间分辨率 荧光检测器 显微CT
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Recent advances in targeted endoscopic imaging:Early detection of gastrointestinal neoplasms
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作者 Yong-Soo Kwon Young-Seok Cho +2 位作者 Tae-Jong Yoon Ho-Shik Kim Myung-Gyu Choi 《World Journal of Gastrointestinal Endoscopy》 CAS 2012年第3期57-64,共8页
Molecular imaging has emerged as a new discipline in gastrointestinal endoscopy.This technology encompasses modalities that can visualize disease-specific morphological or functional tissue changes based on the molecu... Molecular imaging has emerged as a new discipline in gastrointestinal endoscopy.This technology encompasses modalities that can visualize disease-specific morphological or functional tissue changes based on the molecular signature of individual cells.Molecular imaging has several advantages including minimal damage to tissues,repetitive visualization,and utility for conducting quantitative analyses.Advancements in basic science coupled with endoscopy have made early detection of gastrointestinal cancer possible.Molecular imaging during gastrointestinal endoscopy requires thedevelopment of safe biomarkers and exogenous probes to detect molecular changes in cells with high specificity anda high signal-to-background ratio.Additionally,a high-resolution endoscope with an accurate wide-field viewing capability must be developed.Targeted endoscopic imaging is expected to improve early diagnosis and individual therapy of gastrointestinal cancer. 展开更多
关键词 AUTOfluorescence ENDOSCOPY Confocal ENDOMICROSCOPY ENDOSCOPY molecular imaging molecular probes Near-infrared fluorescence imaging TARGETED endoscopic imaging
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PHOTOSWITCHABLE NANOFLUOROPHORES FOR INNOVATIVE BIOIMAGING
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作者 MING-QIANG ZHU GUO-FENG ZHANG +3 位作者 CHONG LI YA-JING LI MATTHEW PALDRED ALEXANDER D.Q.LI 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2011年第4期395-408,共14页
Photosensitive fluorescent probes have become powerful tools in chemical biology and molecular biophysics,which are used to investigate cellular processes with high temporal and spatial resolution.Accordingly,photosen... Photosensitive fluorescent probes have become powerful tools in chemical biology and molecular biophysics,which are used to investigate cellular processes with high temporal and spatial resolution.Accordingly,photosensitive fluorescent probes,including photoactivatable,photoconvertible,and photoswitchable fluorophores,have been extensively developed during the past decade.The photoswitchable fluorophores have received much attention because they highlight cellular events clearly.This minireview summarizes recent advances of using reversibly photoswitchable fluorophores and their applications in innovative bioimaging.Photoswitchable fluorophores include photoswitchable fluorescent proteins,photoswitchable fluorescent organic molecules(dyes),and photoswitchable fluorescent nanoparticles.Several strategies have been developed to synthesize photoswitchable fluorophores,including engineering combination proteins,chemical synthesis,polymerization,and self-assembly.Here we concentrate on polymer nanoparticles with optically switchable emission properties:either fluorescence on/offor dualalternating-color fluorescence photoswitching.The essential mechanisms of fluorescence photoswitching enable different types of photoswitchable fluorophores to change emission intensity or wavelength(color)and thus validating the basis of the fluorescence on/offor dual-color photoswitching design.Generally the possible applications of any fluorophores are to label biological targets,followed by specific imaging.The newly developed photoswitchable fluorophores enable super-resolution fluorescence imaging because of their photosensitive emission.Finally,we summarize the important area regarding future research and development on photoswitchable fluorescent nanoparticles. 展开更多
关键词 Fluorescent molecular switches polymer nanoparticles TWO-PHOTON two-photon imaging SPIROPYRAN
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Nucleic Acid Probes for Single-Molecule Localization Imaging of Cellular Biomolecules
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作者 Junyuan Wei Cailing Ji +3 位作者 Yingfei Wang Jie Tan Quan Yuan Weihong Tan 《Chemical & Biomedical Imaging》 CAS 2023年第1期18-29,共12页
Endogenous biomolecules in cells are the basis of all life activities.Directly visualizing the structural characteristics and dynamic behaviors of cellular biomolecules is signiffcant for understanding the molecular m... Endogenous biomolecules in cells are the basis of all life activities.Directly visualizing the structural characteristics and dynamic behaviors of cellular biomolecules is signiffcant for understanding the molecular mechanisms in various biological processes.Singlemolecule localization microscopy(SMLM)can circumvent the optical diffraction limit,achieving analysis of the ffne structures and biological processes in living cells with nanoscale resolution.However,the large size of traditional imaging probes prevents SMLM from accurately locating ffne structures and densely distributed biomolecules within cells.In recent years,nucleic acid probes have emerged as potential tools to replace conventional SMLM probes by virtue of their small size and high speciffcity.In addition,due to their programmability,nucleic acid probes with different conformations can be constructed via sequence design,further extending the application of SMLM in bioanalysis.Here,we discuss the design concepts of different conformational nucleic acid probes for SMLM and summarize the application of SMLM based on nucleic acid probes in the ffeld of biomolecules.Furthermore,we provide a summary and future perspectives of the nucleic acid probe-based SMLM technology,aiming to provide guidance for the acquisition of nanoscale information about cellular biological processes. 展开更多
关键词 single-molecule localization microscopy super-resolution imaging photoactivated localization microscopy stochastic optical reconstruction microscopy DNA-based point accumulation for imaging in nanoscale topography intracellular molecular interaction nucleic acid probes conformational design biomolecular analysis
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Two-stage Source Reconstruction Algorithm for Bioluminescence Tomography Using Hybrid FEM
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作者 Hou, Yan-Bin Zhao, Heng +3 位作者 Qu, Xiao-Chao Chen, Duo-Fang Wang, Xiao-Rui Liang, Ji-Min 《International Journal of Automation and computing》 EI 2012年第3期225-231,共7页
A two-stage source reconstruction algorithm for bioluminescence tomography (BLT) is developed using hybrid finite element method (FEM). The proposed algorithm takes full advantages of linear and quadratic FEMs, which ... A two-stage source reconstruction algorithm for bioluminescence tomography (BLT) is developed using hybrid finite element method (FEM). The proposed algorithm takes full advantages of linear and quadratic FEMs, which can be used to localize and quantify bioluminescent source accurately. In the first stage, a large permissible region is roughly determined and then iteratively evolved to reduce matrix dimension using efficient linear FEM. In the final stage, high-convergence quadratic FEM is applied to improve reconstruction result. Both numerical simulation and physical experiment are performed to evaluate the proposed algorithm. The relevant results demonstrate that quantitative reconstruction can be well achieved in terms of computation efficiency, source position, power density, and total power when compared with previous studies. 展开更多
关键词 molecular imaging bioluminescence tomography source reconstruction TWO-STAGE finite element method (FEM).
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OPTIMIZATION OF DESIGN PARAMETERS FOR FLUORESCENCE LAMINAR OPTICAL TOMOGRAPHY
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作者 CHAO-WEI CHEN YU CHEN 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2011年第3期309-323,共15页
Laminar optical tomography(LOT)is a mesoscopic tomographic imaging technique ranging between confocal microscopy and diffuse optical tomography(DOT).Fluorescence LOT(FLOT)provides depth-resolved molecular information ... Laminar optical tomography(LOT)is a mesoscopic tomographic imaging technique ranging between confocal microscopy and diffuse optical tomography(DOT).Fluorescence LOT(FLOT)provides depth-resolved molecular information with 100-200μm resolution over 2-3mm depth.In this study,we use Monte Carlo simulation and singular-value analysis(SVA)to optimize the source-detector configurations for potential enhancement of FLOT imaging performance.The effects of different design parameters,including source incidence and detector collection angles,detector number,and sampling density,are presented.The results indicate that angled incidence/detection configuration might improve the imaging resolution and depth sensitivity,especially for low-scattering medium.Increasing the number of detectors and the number of scanning steps will also result in enhanced imaging performance.We also demonstrate that the optimal imaging performance depends upon the background scattering coe±cient.Our result might provide an optimization strategy for FLOT or LOT experimental setup. 展开更多
关键词 Laminar optical tomography(LOT) fluorescence laminar optical tomography(FLOT) singular value analysis(SVA) image reconstruction
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Activatable molecular fluorescence probes for the imaging and detection of ischemic stroke 被引量:1
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作者 Mengdie Wang Yan Zhang 《Brain Science Advances》 2023年第1期35-42,共8页
The real-time, noninvasive, nonionizing, high spatiotemporal resolution, and flexibility characteristics of molecular fluorescence imaging provide a uniquely powerful approach to imaging and monitoring the physiology ... The real-time, noninvasive, nonionizing, high spatiotemporal resolution, and flexibility characteristics of molecular fluorescence imaging provide a uniquely powerful approach to imaging and monitoring the physiology and pathophysiology of ischemic stroke. Currently, various fluorescence probes have been synthesized with the aim of improving quantitative and quantitative studies of the pathologic processes of ischemic stroke in living animals. In this review, we present an overview of current activatable fluorescence probes for the imaging and diagnosis of ischemic stroke in animal models. We categorize the probes based on their activatable signals from the biomarkers associated with ischemic stroke, and we present representative examples of their functional mechanisms. Finally, we briefly discuss future perspectives in this field. 展开更多
关键词 molecular imaging PROTEASE metal ion ROS fluorescence probe ischemic stroke DETECTION
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Fluorescence lifetime imaging of molecular rotors to map microviscosity in cells
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作者 James A. Levitt Marina K. Kuimova +3 位作者 Gokhan Yahioglu Pei-Hua Chung Klaus Suhling David Phillips 《Chinese Optics Letters》 SCIE EI CAS CSCD 2010年第10期926-930,共5页
Fluorescence liftime imaging (FLIM) of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their envi... Fluorescence liftime imaging (FLIM) of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their environment. Incubating cells with these dyes, we find a punctate and continuous distribution of the dye in cells. The viscosity value obtained in what appears to be endocytotic vesicles in living cells is around 100 times higher than that of water and of cellular cytoplasm.Time-resolved fluorescence anisotropy measurements also yield rotational correlation times consistent with large microviscosity values. In this way, we successfully develop a practical and versatile approach to map the microviscosity in cells based on imaging fluorescent molecular rotors. 展开更多
关键词 fluorescence lifetime imaging of molecular rotors to map microviscosity in cells
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荧光共价有机框架的合成及其传感、成像应用研究进展
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作者 刘俊杰 张颖 罗志敏 《福建师范大学学报(自然科学版)》 CAS 北大核心 2024年第1期1-13,共13页
荧光共价有机框架(fluorescent covalent organic frameworks,FCOFs)由各种荧光有机分子单体通过可逆缩合反应形成高度有序的结晶性有机框架结构,具有独特的荧光特性、良好的化学稳定性、高比表面积、大π共轭结构以及有序的永久性可调... 荧光共价有机框架(fluorescent covalent organic frameworks,FCOFs)由各种荧光有机分子单体通过可逆缩合反应形成高度有序的结晶性有机框架结构,具有独特的荧光特性、良好的化学稳定性、高比表面积、大π共轭结构以及有序的永久性可调节孔隙等特点,在传感和成像等领域受到越来越多的关注。综述了FCOFs的定义、光学性质、合成方法及其在传感与成像方面的应用研究进展,并对FCOFs材料的设计合成及应用面临的挑战进行了展望。 展开更多
关键词 共价有机框架 荧光 传感 成像 药物载体 分子检测 生物诊疗
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基于荧光分子间距和数量的原始图像去噪研究
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作者 许聪 程涛 《广西科技大学学报》 CAS 2024年第4期100-107,共8页
在随机光学重建显微(stochastic optical reconstruction microscopy,STORM)的原始图像采集过程中,噪声是不可避免的。块匹配三维滤波是一种优秀的去噪算法。由于衍射现象,每个荧光分子都会在原始图像中形成弥散斑。不同的荧光分子间距... 在随机光学重建显微(stochastic optical reconstruction microscopy,STORM)的原始图像采集过程中,噪声是不可避免的。块匹配三维滤波是一种优秀的去噪算法。由于衍射现象,每个荧光分子都会在原始图像中形成弥散斑。不同的荧光分子间距会形成弥散斑重叠程度不一样的原始图像。针对块匹配三维滤波对原始图像去噪能力与荧光分子间距的关系问题,提出以不同分子间距的原始图像为研究对象的方法。将不同分子间距的原始图像分为弥散斑中心区域发生重叠和不重叠两类。研究发现,块匹配三维滤波可以有效地去除原始图像的噪声;去噪能力不但与噪声类型和水平有关,而且随荧光分子间距变化。当弥散斑中心区域发生重叠时,去噪前原始图像的信噪比(signal-noise ratio,SNR)与荧光分子数无关;去噪后低噪和中噪原始图像的SNR与荧光分子数有关。 展开更多
关键词 超分辨率显微 原始图像 荧光分子间距 去噪 弥散斑
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