The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticl...The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The fluorescence nanoparticles immune based lateral flow strips are a little of sample can be detected fast, easily, inexpensive, easy to universal without professional technical personnel detection method. It provides a new detection method for the detections of Salmonella pullorum.展开更多
We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was ...We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.展开更多
利用反相微乳技术合成了稀土铕-S iO2纳米颗粒,用其与氯霉素单克隆抗体结合制备氯霉素类残留物免疫检测探针,并制备免疫层析试纸条,用于牛奶中氯霉素和氯霉素琥珀酸盐同步检测,简便快速,30 m in内出结果。与甲砜霉素、庆大霉素、磺胺二...利用反相微乳技术合成了稀土铕-S iO2纳米颗粒,用其与氯霉素单克隆抗体结合制备氯霉素类残留物免疫检测探针,并制备免疫层析试纸条,用于牛奶中氯霉素和氯霉素琥珀酸盐同步检测,简便快速,30 m in内出结果。与甲砜霉素、庆大霉素、磺胺二甲基嘧啶之间无交叉反应,特异性好,未发现非特异性吸附问题。展开更多
文摘The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The fluorescence nanoparticles immune based lateral flow strips are a little of sample can be detected fast, easily, inexpensive, easy to universal without professional technical personnel detection method. It provides a new detection method for the detections of Salmonella pullorum.
基金supported by the National Natural Science Foundation of China(22236002).
文摘We report the development of a sensitive,fast,and simple method for the detection of prometryn(PRO)in potato and celery using a lateral flow immunochromatographic assay(LFIA).A monoclonal antibody(mAb)against PRO was prepared under optimized conditions,and the 50%inhibition concentration(IC50)for use in ELISA was determined to be 0.6 ng mL−1.Moreover,the cut-off value for use in an LFIA was 50 ng mL−1.To validate the reliability of the developed LFIA strip,real samples were used,and the recovery rate was found to be 99.3-104.3%.The assays were completed within 8 min,demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.
