Objective Improper land disposal of hazardous waste can result in leaching of hazardous constituents which may contaminate ground and surface water leading to adverse impact on human health and environment consequenc...Objective Improper land disposal of hazardous waste can result in leaching of hazardous constituents which may contaminate ground and surface water leading to adverse impact on human health and environment consequences. The present study utilized mammalian cell culture for the genotoxicity assessment of waste and its leachate. Methods Genotoxic potential and chemical analysis of pesticide derived tarry waste contaminated soil extract and its leachate was assessed using in vitro human lymphocyte cultures and GC-MS. Results The investigation revealed that the soil extract could cause significant to highly significant genotoxicity in the form of DNA strand break at 25 mL (P<0.01), 50 mL, 100 mL and 200 mL (P<0.001) and chromosomal aberration at 25 mL (P<0.01) and 50 mL and 100 mL (P<0.001). The leachate could cause significant DNA strand break and chromosomal aberration only at 100 mL and 200 mL (P<0.01) dose levels. Conclusion The genotoxicity observed is attributed to carbaril and tetra methyl naphthyl carbamate, the major ingredients of the extracts, as revealed by GC-MS.展开更多
简化和改进了 DNA 解旋的荧光法,并用于辐射与平阳霉素所造成的 DNA 链断裂的检测。获得了不同剂量范围γ线照射所致 HeLa S3细胞 DNA 链断裂的线性效应曲线,最小检出剂量为0.25Gy。观察了不同剂量γ线照射后 DNA 链断裂的重接修复。并...简化和改进了 DNA 解旋的荧光法,并用于辐射与平阳霉素所造成的 DNA 链断裂的检测。获得了不同剂量范围γ线照射所致 HeLa S3细胞 DNA 链断裂的线性效应曲线,最小检出剂量为0.25Gy。观察了不同剂量γ线照射后 DNA 链断裂的重接修复。并检测了平阳霉素在不同浓度不同温度下处理 HeLa S3细胞所致 DNA 链断裂。展开更多
文摘Objective Improper land disposal of hazardous waste can result in leaching of hazardous constituents which may contaminate ground and surface water leading to adverse impact on human health and environment consequences. The present study utilized mammalian cell culture for the genotoxicity assessment of waste and its leachate. Methods Genotoxic potential and chemical analysis of pesticide derived tarry waste contaminated soil extract and its leachate was assessed using in vitro human lymphocyte cultures and GC-MS. Results The investigation revealed that the soil extract could cause significant to highly significant genotoxicity in the form of DNA strand break at 25 mL (P<0.01), 50 mL, 100 mL and 200 mL (P<0.001) and chromosomal aberration at 25 mL (P<0.01) and 50 mL and 100 mL (P<0.001). The leachate could cause significant DNA strand break and chromosomal aberration only at 100 mL and 200 mL (P<0.01) dose levels. Conclusion The genotoxicity observed is attributed to carbaril and tetra methyl naphthyl carbamate, the major ingredients of the extracts, as revealed by GC-MS.
文摘简化和改进了 DNA 解旋的荧光法,并用于辐射与平阳霉素所造成的 DNA 链断裂的检测。获得了不同剂量范围γ线照射所致 HeLa S3细胞 DNA 链断裂的线性效应曲线,最小检出剂量为0.25Gy。观察了不同剂量γ线照射后 DNA 链断裂的重接修复。并检测了平阳霉素在不同浓度不同温度下处理 HeLa S3细胞所致 DNA 链断裂。
