AIM:To observe the shifting hierarchy of the conjunctival florae in the patients who employed a long-time topical fluoroquinolone and characterize the consequent variations of their antibiotic sensitivity and virulenc...AIM:To observe the shifting hierarchy of the conjunctival florae in the patients who employed a long-time topical fluoroquinolone and characterize the consequent variations of their antibiotic sensitivity and virulence.METHODS:A total of 143 eyes(143 patients)who suffered from the non-infectious corneal ulcer and topically used fluoroquinolone more than 2 wk were enrolled as the fluoroquinolone eye.The untreated fellow eye was considered as the contralateral eye.Seventy-five healthy subjects were selected as the control.The culture positivity and strains of the isolated conjunctival florae were observed.Their antibiotic susceptibility and expression of the virulence-related genes were detected.RESULTS:Florae were recovered from 84.0%,37.1%,and 57.3%of the conjunctival swabs in the control,fluoroquinolone eye,and contralateral eye,respectively.The most frequently isolated microorganisms were Staphylococcus epidermidis(34.9%)in the control,followed by Staphylococcus aureus(17.5%),Staphylococcus saprophyticus(14.3%),Micrococcus(9.5%),Propionibacterium acnes(7.9%).However,those orderly ranks shifted to Staphylococcus aureus(34.0%),Propionibacterium acnes(20.8%),Candida albicans(17.0%),Pseudomonas aeruginosa(9.4%)in the fluoroquinolone eye.A growing number of the fluoroquinolone-resistant florae survived in the fluoroquinolone eye,accompanied by an increased expression of the virulence-related genes.CONCLUSION:A long-time topical fluoroquinolone leads to a shifting hierarchy of the conjunctival florae,accompanied by the consequent variations of the antibiotic sensitivity and virulence.展开更多
Objective: To investigate factors contributed to anti-infection efficacy of fluoroquinolones (FQNS), and ultimately to provide guidelines for the application of such drugs. Methods: Clinical data of 519 infected patie...Objective: To investigate factors contributed to anti-infection efficacy of fluoroquinolones (FQNS), and ultimately to provide guidelines for the application of such drugs. Methods: Clinical data of 519 infected patients who were treated with fluoroquinolones were analyzed retrospectively. According to the therapeutic efficacy of the drugs, cases were divided into 3 groups: clinical inefficient, improved and cured. 11 potential factors were investigated. The data were analyzed through logistic regression analysis to determine the main factors which influence therapeutic effects. Results: Ordinal logistic regression revealed that age (OR = 0.979, 95% CI: 0.969, 0.989), a variety of medicine (moxifloxacin-OR = 3.465, 95% CI: 1.396, 8.601;levofloxacin-OR = 4.605, 95% CI: 1.971, 10.760;ciprofloxacin-OR = 3.220, 95% CI: 1.089, 9.552;compared to lomefloxacin) (levofloxacin-OR = 2.591, 95% CI: 1.130, 5.944;compared to fleroxacin) and site of infection (respiratory system-OR = 3.016, 95% CI: 1.737, 5.236;urological system-OR = 4.077, 95% CI: 1.981, 8.391;digestive system-OR = 3.740, 95% CI: 1.849, 7.565) are main factors which influence the efficacy. Conclusion: Fluoroquinolones are more effective in the treatment of bacterial infection within drug’s indications in young population. Variety, dosage and intervals of the drugs should be adjusted according to disease condition.展开更多
基金Supported by the National Natural Science Foundation of China(No.81870631)。
文摘AIM:To observe the shifting hierarchy of the conjunctival florae in the patients who employed a long-time topical fluoroquinolone and characterize the consequent variations of their antibiotic sensitivity and virulence.METHODS:A total of 143 eyes(143 patients)who suffered from the non-infectious corneal ulcer and topically used fluoroquinolone more than 2 wk were enrolled as the fluoroquinolone eye.The untreated fellow eye was considered as the contralateral eye.Seventy-five healthy subjects were selected as the control.The culture positivity and strains of the isolated conjunctival florae were observed.Their antibiotic susceptibility and expression of the virulence-related genes were detected.RESULTS:Florae were recovered from 84.0%,37.1%,and 57.3%of the conjunctival swabs in the control,fluoroquinolone eye,and contralateral eye,respectively.The most frequently isolated microorganisms were Staphylococcus epidermidis(34.9%)in the control,followed by Staphylococcus aureus(17.5%),Staphylococcus saprophyticus(14.3%),Micrococcus(9.5%),Propionibacterium acnes(7.9%).However,those orderly ranks shifted to Staphylococcus aureus(34.0%),Propionibacterium acnes(20.8%),Candida albicans(17.0%),Pseudomonas aeruginosa(9.4%)in the fluoroquinolone eye.A growing number of the fluoroquinolone-resistant florae survived in the fluoroquinolone eye,accompanied by an increased expression of the virulence-related genes.CONCLUSION:A long-time topical fluoroquinolone leads to a shifting hierarchy of the conjunctival florae,accompanied by the consequent variations of the antibiotic sensitivity and virulence.
文摘Objective: To investigate factors contributed to anti-infection efficacy of fluoroquinolones (FQNS), and ultimately to provide guidelines for the application of such drugs. Methods: Clinical data of 519 infected patients who were treated with fluoroquinolones were analyzed retrospectively. According to the therapeutic efficacy of the drugs, cases were divided into 3 groups: clinical inefficient, improved and cured. 11 potential factors were investigated. The data were analyzed through logistic regression analysis to determine the main factors which influence therapeutic effects. Results: Ordinal logistic regression revealed that age (OR = 0.979, 95% CI: 0.969, 0.989), a variety of medicine (moxifloxacin-OR = 3.465, 95% CI: 1.396, 8.601;levofloxacin-OR = 4.605, 95% CI: 1.971, 10.760;ciprofloxacin-OR = 3.220, 95% CI: 1.089, 9.552;compared to lomefloxacin) (levofloxacin-OR = 2.591, 95% CI: 1.130, 5.944;compared to fleroxacin) and site of infection (respiratory system-OR = 3.016, 95% CI: 1.737, 5.236;urological system-OR = 4.077, 95% CI: 1.981, 8.391;digestive system-OR = 3.740, 95% CI: 1.849, 7.565) are main factors which influence the efficacy. Conclusion: Fluoroquinolones are more effective in the treatment of bacterial infection within drug’s indications in young population. Variety, dosage and intervals of the drugs should be adjusted according to disease condition.
