聚焦离子束连续切片扫描电镜(focused ion beam serial block face scanning electron microscopy,FIB-SEM)技术,目前已被广泛应用于小体积细胞或组织样品的三维重构,具有自动化程度高、Z轴分辨高等优点。本文从包埋块样品准备与处理、...聚焦离子束连续切片扫描电镜(focused ion beam serial block face scanning electron microscopy,FIB-SEM)技术,目前已被广泛应用于小体积细胞或组织样品的三维重构,具有自动化程度高、Z轴分辨高等优点。本文从包埋块样品准备与处理、样品区域选定、软件设置前准备、软件参数(离子束加工和电子束扫描)设置、软件运行与图像采集和图像处理等多个方面,详细介绍应用FIB-SEM技术对常温生物包埋块样品进行三维重构的流程和细节,并对某些关键性参数展开讨论。展开更多
Ning et al. （2015） developed a 2D fully nonlinear potential model to investigate the interaction between focused waves and uniform currents. The effects of uniform current on focusing wave crest, focal time and foca...Ning et al. （2015） developed a 2D fully nonlinear potential model to investigate the interaction between focused waves and uniform currents. The effects of uniform current on focusing wave crest, focal time and focal position were given. As its extension, harmonic energy transfer for focused waves in uniform current is studied using the proposed model by Ning et al. （2015） and Fast Fourier Transformation （FFT） technique in this study. It shows that the strong opposing currents, inducing partial wave blocking and reducing the extreme wave crest, make the nonlinear energy transfer non-reversible in the focusing and defocusing processes. The numerical results also provide an explanation to address the shifts of focal points in consideration of the combination effects of wave nonlinearity and current.展开更多
文摘聚焦离子束连续切片扫描电镜(focused ion beam serial block face scanning electron microscopy,FIB-SEM)技术,目前已被广泛应用于小体积细胞或组织样品的三维重构,具有自动化程度高、Z轴分辨高等优点。本文从包埋块样品准备与处理、样品区域选定、软件设置前准备、软件参数(离子束加工和电子束扫描)设置、软件运行与图像采集和图像处理等多个方面,详细介绍应用FIB-SEM技术对常温生物包埋块样品进行三维重构的流程和细节,并对某些关键性参数展开讨论。
基金financially supported by the National Natural Science Foundation of China(Grant Nos.51679036 and 51490672)the Royal Academy of Engineering under the UK-China Industry Academia Partnership Programme(Grant No.UK-CIAPP\73)the Program for New Century Excellent Talents in University(Grant No.NCET-13-0076)
文摘Ning et al. （2015） developed a 2D fully nonlinear potential model to investigate the interaction between focused waves and uniform currents. The effects of uniform current on focusing wave crest, focal time and focal position were given. As its extension, harmonic energy transfer for focused waves in uniform current is studied using the proposed model by Ning et al. （2015） and Fast Fourier Transformation （FFT） technique in this study. It shows that the strong opposing currents, inducing partial wave blocking and reducing the extreme wave crest, make the nonlinear energy transfer non-reversible in the focusing and defocusing processes. The numerical results also provide an explanation to address the shifts of focal points in consideration of the combination effects of wave nonlinearity and current.
