Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats （Caprus hircus） is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As a...Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats （Caprus hircus） is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes （HFDRGs） in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxcl3 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFP- Hoxc13. The result suggested that overexpression ofHoxcl3 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.展开更多
Young female cancer survival rates significantly increased due to the great progress of cancer therapy.In fact,cryostorage and transplantation of ovarian tissue have already resulted in the birth of healthy babies.Fol...Young female cancer survival rates significantly increased due to the great progress of cancer therapy.In fact,cryostorage and transplantation of ovarian tissue have already resulted in the birth of healthy babies.Follicle in vitro growth(IVG)has the great potential of restoring fertility by achieving functional oocytes from the most immature stages to maturation.This is suitable for a wide range of patients,from pubertal to perimenopause women.Notable achievements have been achieved in human follicle IVG in the past decade.Mature oocytes have been successfully collected from long-term sequential follicle IVG.However,it is still a major challenge to establish a stable and efficient follicle IVG system able to generate mature and competent oocytes.Hereby,we review the approaches being taken so far using ovarian tissue to support follicle growth at different stages in vitro.展开更多
基金the National Natural Science Foundation of China (30960246)the Key Project of National Science and Technology Pillar Program of China (2011BAD28B05)+3 种基金the National High Technology Research and Development Program of China (2007AA10Z151)the Specialized Research Fund for the Doctoral Program of Higher Education(20091515120010)the Inner Mongolia Natural Science Foundation, China (20080404ZD04)the China Agriculture Research System (CARS-40)
文摘Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats （Caprus hircus） is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes （HFDRGs） in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxcl3 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFP- Hoxc13. The result suggested that overexpression ofHoxcl3 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.
文摘Young female cancer survival rates significantly increased due to the great progress of cancer therapy.In fact,cryostorage and transplantation of ovarian tissue have already resulted in the birth of healthy babies.Follicle in vitro growth(IVG)has the great potential of restoring fertility by achieving functional oocytes from the most immature stages to maturation.This is suitable for a wide range of patients,from pubertal to perimenopause women.Notable achievements have been achieved in human follicle IVG in the past decade.Mature oocytes have been successfully collected from long-term sequential follicle IVG.However,it is still a major challenge to establish a stable and efficient follicle IVG system able to generate mature and competent oocytes.Hereby,we review the approaches being taken so far using ovarian tissue to support follicle growth at different stages in vitro.
