Proteome changes of porcine follicular fluid during follicle development

Background:Ovarian follicular fluid influences follicle and oocyte growth,but the fluctuation of its protein content during folliculogenesis has not been comprehensively analyzed.Here we used a shotgun approach and bioinformatics analyses to investigate and compare the proteomes of porcine follicular fluid(pFF)obtained from small(<4 mm),medium(4–6 mm)and large(>6–12 mm)follicles.Results:Follicular fluid samples containing highest estrogen levels were selected as non-atretic from small(SNA:26.1±15 ng/mL),medium(MNA:162±54 ng/mL),and large(LNA:290±37 ng/mL)follicles for proteomic analyses.We detected 1627,1699,and 1756 proteins in SNA,MNA,and LNA samples,respectively.Nearly 60–63%of total proteins were specific to each sample,11–13%were shared in pairwise comparisons,and 247 proteins were shared among all samples.Functional categorization indicated comparable gene ontology(GO)terms distribution per cellular component,molecular function,and biological process categories across samples;however,the ranking of highly significantly enriched GO terms per category revealed differences between samples.The patterns of proteinto-protein interactions varied throughout follicle development,and proteins such as serine protease inhibitor,clade E(SERPINE);plasminogen activator,urokinase(PLAU);and plasminogen activator,urokinase receptor(PLAUR)appeared stage-specific to SNA,MNA,and LNA,respectively.The“complement and coagulation cascades”was the common major pathway.Besides,properdin and fibulin-1 were abundant proteins that appeared absent in LNA samples.Conclusion:This study provides extensive and functional analyses of the pFF proteome changes during folliculogenesis and offers the potential for novel biomarker discovery in pFF for oocyte quality assessment.

Association of the microbial culture of follicular fluid,vaginal swab and catheter tip with β-hCG IVF positive and negative

Objective:To find out the association of microbial contamination withβ-human chorionic gonadotropin(β-hCG)in-vitro fertilization(IVF)positive and negative.Methods:A total of 73 fresh IVF cycle women were included in the retrospective study.Vaginal swab culture samples were collected prior to ovum pick-up and embryo transfer.The follicular fluids were collected during ovum pick-up and catheter tip culture samples were collected after successful embryo transfer.After 14 days of the embryo transfer,women were classified intoβ-hCG IVF positive and negative.The comparative statistical analyses of aerobic microbial culture reports were done betweenβ-hCG IVF positive and negative women.Results:Out of 73 women,42(57.5%)were found to beβ-hCG IVF positive and 31(43.5%)were negative.In the aerobic culture of ovum pick-up vaginal swab,follicular fluid,embryo transplantation vaginal swab and catheter tip,Enterococcus faecalis was found to be higher compared to other bacteria(Streptococcus spp.,Candida,Escherichia coli and Klebsiella).Regarding the comparison between IVF positive and negative,the overall microbial infection rate of vaginal swab culture during ovum pick-up and embryo transplantation was found to be higher in IVF negative women than in IVF positive women(38.71%vs.28.57%);however,it was not statistically significant(P>0.05).The follicular fluid and catheter tip culture microbial infection rate was found to slightly higher in IVF positive women than in IVF negative women(54.76%vs.41.94%and 19.05%vs.9.68%,respectively),but there were not significant differences(P>0.05).Conclusions:The aerobic microbial culture reports of follicular fluid,vaginal swab culture,and catheter tip culture are not statistically significantly withβ-hCG IVF positive.

Influence of Follicular Fluid on <i>in Vitro</i>Maturation and Fertilization of Bovine Oocytes

The aim of this study was to investigate the effect of time on in vitro maturation of bovine oocytes and of the addition of follicular fluid on meiotic progression. The cumulus-oocyte complexes (COCs) collected from 3 to 6 mm follicles were obtained from ovaries of slaughtered female animals. The medium of maturation was supplemented or not with 20 μL follicular fluid (FF);661 oocytes were matured in vitro (extrusion of the first polar corpuscle) for 22 hours with added follicular fluid (AFF) (72.01%) or without follicular fluid (WFF) (67.53%) and 679 oocytes were matured in vitro for 26 hours (extrusion of the first polar corpuscle) with AFF (92.1%) and WFF (77.15%). The results of extrusion of the second polar corpuscle as an event related to the fertilization percentages showed that the increase in the fertilization rate is maintained at 26 hours with AFF (79.45%), but the percentage decreases WFF (65.08%). After 22 hours, the fertilization rate was 62.38% AFF and 53.40% WFF. The developmental competence of bovine oocytes is affected by the duration of maturation in vitro and the inclusion in the FF culture medium. The use of follicular fluid in the in vitro maturation medium may be a biological strategy to increase the cumulus expansion, the nuclear maturation and the in vitro fertilization.

Effect of Follicular Fluid Lactoferrin Level on Oocytes Quality and Pregnancy Rate in Intracytoplasmic Sperm Injection Cycles

Assessment of oocyte quality to avoid overproduction of embryos is now considered an important goal in ICSI cycles. Although in classic Intra Cytoplasmic Sperm Injection (ICSI) cycles selection is mainly done for embryos and not for follicles. Follicular fluid and its contents representing the oocyte environment are now gaining more attention because not only of its crucial influence on oocyte developments but also due to easy isolation of follicular fluid with every case of ICSI. By its aspiration during ovum pick up (OPU). substances isolated from follicular fluid to assess oocyte quality were in the form of: hormones like, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Anti-mullerian Hormon (AMH), Growth Hormone (GH) etc., growth factors like Insulin Like Growth Factor (ILGF), proteins and amino acids like Lactoferrin (LF) which is an iron-binding glycoprotein that was detected in follicular fluid and was thought to be related to good oocyte quality when present in high concentration. Objectives: The aim of this study was to detect the possible effect of follicular fluid lactoferrin on oocyte quality and hence pregnancy rate in ICSI cycles. Methods: Follicular fluid was obtained from 64 patients undergoing Intra-cytoplasmic sperm injection (ICSI) procedure in Ain Shams University Maternity Hospital. Follicular fluid (FF) was collected at the time of oocyte harvesting. The lactoferrin concentration in FF was assayed by ELISA. Results: The mean LF concentration in follicular fluid of the positive biochemical pregnancy group (0.63 ± 0.17 ng/mL) was not significantly higher than that in the negative biochemical pregnancy (0.61 ± 0.16 ng/mL). A positive correlation between a number of mature oocytes and lactoferrin concentration was not found (r.101). Conclusion: Lactoferrin level in follicular fluid does not correlate with oocytes quality or pregnancy rate.

Follicular fluid levels of prostaglandin E2 and the effect of prostaglandin E2 on steroidogenesis in granulosa-lutein cells in women with moderate and severe endometriosis undergoing in vitro fertilization and embryo transfer

Background The mechanisms of endometriosis with infertility have not been fully studied. The present study aimed to assess the follicular fluid （FF） levels of prostaglandin E2 （PGE2）, which plays a critical role within the ovary, and to investigate the effect of PGE2 on steroidogenesis in granulosa-lutein cells （GLCs） from women with and without endometriosis. Methods Thirty-three women with laparoscopically documented endometriosis and 40 controls undergoing in vitro fertilization （IVF） were studied. We assayed the concentrations of PGE2 in FF, the production of E2 and progesterone in FF and in culture medium, and the expression of steroidogenic acute regulatory protein （STAR） and CYP19A1 in GLCs with the intervention of PGE2. Results PGE2 and progesterone concentrations were increased and displayed positive correlation in endometriotic FE PGE2 induced the expression of STAR and the production of progesterone in GLCs from women with endometriosis, and the expression of STAR and the production of progesterone were increased in GLCs from women with endometriosis. However, there were no significant effects of PGE2 on promoting the production of E2 or the expression of CYP19A1 in GLCs. Moreover, the production of E2 and the expression of CYP19A1 in GLCs from women with endometriosis were significantly decreased compared to the controls. Conclusions PGE2 concentrations are increased in endometriotic FF, along with concomitant increases in progesterone and STAR. In contrast, the E2 and CYP19A1 are decreased in GLCs, which may delay the development of the follicles and cause an imbalance in the follicular steroid hormone levels. These changes may have close relationship with endometriosis-associated infertility.

子宫内膜异位症患者卵泡液外泌体miRNA谱差异及生信分析

目的探讨子宫内膜异位症(EMT)患者卵泡液来源的外泌体差异微小RNA(miRNA)对卵母细胞质量的影响。方法收集2021年12月—2022年3月在广州市第一人民医院生殖医学中心进行体外受精-胚胎移植/卵细胞浆内单精子注射助孕的20例不孕症患者的卵泡液,分为EMT组(EMT不孕症患者10例)和对照组(单纯男性因素不孕症患者10例)。采用高通量测序对卵泡液外泌体微小RNA(miRNA)谱进行分析,选出具有组间差异的miRNAs。结果与单纯男性因素不孕患者相比,EMT组有18个外泌体miRNAs差异有统计学意义,其中上调9个、下调9个。靶基因预测并采用GO和KEGG富集分析发现,这些靶基因主要参与磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K-Akt)、核苷酸结合寡聚结构域NOD样受体、Ras等信号通路。结论EMT患者卵泡液来源的外泌体miRNA存在差异,差异的外泌体miRNAs可能通过多个信号通路影响EMT患者卵母细胞质量。

猪卵泡液外泌体处理卵巢颗粒细胞的SNP/Indel筛选分析

旨在分析卵泡液外泌体对卵巢颗粒细胞基因的影响,了解卵泡液外泌体在卵泡发育过程中的调控机理,为母猪繁殖研究提供理论依据。本研究选取6月龄、健康状态良好且体重相近的二元母猪为材料,屠宰后收集卵巢150枚,利用梯度离心法获取卵泡液外泌体与猪卵巢颗粒细胞(porcine ovarian granulosa cells,POGCs),并在体外将卵泡液外泌体与猪卵巢颗粒细胞共培养。通过RNA-seq技术对猪卵巢颗粒细胞(granulosa cell samples,GC,n=3)和与外泌体共培养的猪卵巢颗粒细胞(granulosa-exosome co-culture samples,GCE,n=3)进行测序。结果显示,在GC和GCE组中平均每个样品获得5.54×10^(7)条clean reads,Q20和Q30质量得分均在92%以上。在GC和GCE组的6个样品中共获得1310979个SNPs突变和104498个InDel突变,其中纯合型SNP/Indel突变数量为170426个,杂合型SNP/Indel突变数量为1245052个,突变杂合子数目明显高于纯合子且SNP的发生率较高。另外,在突变类型中,转换类型共计973003个,颠换类型339974个,转换的类型显著高于颠换类型。经基因注释,突变主要发生在基因3′UTR、5′UTR、内含子区域,其次为外显子和基因间隔区。另外,通过与差异基因对比后,够筛选出1583个候选基因,经GO和KEGG功能富集发现,候选基因主要与细胞周期以及细胞增殖/凋亡过程相关。此外,共发现14个与细胞周期、增殖/凋亡通路相关的关键候选基因,其中11个基因存在互作关系。本研究获得的这些SNP/Indel信息可为后续研究外泌体在母猪生殖上的调控奠定科学基础。

多囊卵巢综合征患者卵泡液代谢组学的研究进展

多囊卵巢综合征(PCOS)是一种复杂的异质性内分泌疾病,育龄妇女发病率约为5%~18%,但病因和病理生理仍不清楚。PCOS是无排卵性不孕症最常见的病因,接受体外受精-胚胎移植(IVF-ET)的PCOS患者常存在卵巢过度刺激综合征发生率高、受精率低、流产率高等问题。卵泡液代谢物可为各个阶段的卵母细胞提供养分,小分子代谢物改变会严重影响卵母细胞的生长以及胚胎发育潜能。本文就PCOS患者卵泡液中的各类代谢物进行综述,以期为研究PCOS患者卵母细胞发育潜能低下的发病机制以及寻找更有效的药物作用靶点提供有效证据。

卵泡液外泌体在卵泡细胞功能调节中的机制研究进展

卵泡液是卵泡细胞生长发育的内环境,其成分的变化与卵泡细胞的功能状态息息相关。近年来,人们发现在许多生物体液中都存在一种直径为30~150 nm的双层膜囊泡结构——外泌体(exosome),并揭示了它在众多生理病理过程中的媒介作用。研究者也在卵泡液中鉴定出了外泌体成分,并发现它与卵泡细胞的生长发育有着密切的联系,并间接影响了卵母细胞状态,这对评估卵母细胞质量有着重要意义。

卵母细胞及早期胚胎脂代谢的研究进展

脂代谢为卵母细胞发育成熟及早期胚胎发育过程提供能量,且代谢中间产物具有多种细胞生物学功能,包括生物膜构建、细胞信号传导、类固醇前体产生和代谢。卵母细胞还可以通过卵丘细胞从卵泡液获得脂质及其代谢物,尤其是胆固醇、高密度脂蛋白、甘油三酯、游离脂肪酸、载脂蛋白A1、肉碱。多囊卵巢综合征(PCOS)、肥胖、高龄、代谢紊乱、卵巢低反应(POR)等人群中卵母细胞脂代谢的异常与卵母细胞及其胚胎质量差密切相关。本文将综述卵母细胞减数分裂及早期胚胎发育过程中的脂代谢,为不孕症女性和高龄女性的个体化辅助生殖治疗提供理论支持,也有益于创新与改进未成熟卵母细胞体外培养液。

Follicular hyperandrogenism and insulin resistance in polycystic ovary syndrome patients with normal circulating testosterone levels

Polycystic ovary syndrome（PCOS） is a common reproductive disease with high heterogeneity. The role of excess androgen in PCOS etiology remains disputed, since around 20%-50% of PCOS women do not display hyperandrogenemia. The microenvironment of the ovary critically influences follicular development. In the present study, we assessed the role of androgen in PCOS by investigating whether excessive follicular fluid androgen was present in PCOS patients with normal serum androgen levels and influenced by follicular fluid insulin resistance（IR）.Follicular fluid samples of 105 women with PCOS and 105 controls were collected. Levels of steroid hormones,glucose and insulin in the follicular fluid were examined and compared with data from serum biochemistry tests. We found that 64.9%（63/97） of PCOS patients with normal serum androgen levels displayed abnormally high follicular fluid androgen level. The follicular fluid androgen level was positively correlated with follicular fluid IR within a certain range and follicular fluid estrogen-to-testosterone（E2/T） ratio was significantly reduced in these patients.These results indicated that there existed a subgroup of PCOS patients who displayed excessive follicular fluid androgen and IR despite their normal circulating testosterone（T） levels. Our study highlights the importance of ovary hyperandrogenism and IR in the etiology of PCOS.

多囊卵巢综合征患者miRNAs差异表达的研究进展

微小核糖核酸(microRNA,miRNAs)是短链非编码RNA,在组织发育和分化过程中对转录后基因表达的调节至关重要。它们参与多种生物调节,并且在女性生殖系统参与卵母细胞成熟和卵泡发育,尤其在多囊卵巢综合征(polycystic ovary syndrome,PCOS)中观察到了miRNAs水平的改变。近年来在多囊卵巢综合征患者的颗粒细胞、卵泡液以及外周血中均发现miRNAs有差异性表达,推测它可能是一种潜在的生物标志物,并可能成为诊断和治疗多囊卵巢综合征的新的靶点。本文就miRNAs与多囊卵巢综合征关系的最新研究进展进行综述,并探讨其在多囊卵巢综合征的发病机制以及临床诊断中的潜在作用。

动物卵泡液外泌体分离方法及主要生殖功能研究进展

外泌体(exosome)是一种广泛存在于各种体液环境和部分组织中的纳米级、均一性膜性囊泡,主要由蛋白质、脂质、mRNA、miRNA和lncRNA等多种生物成分构成。近年来,研究学者已从不同动物的卵泡液中分离出外泌体,并证实卵泡液外泌体参与卵泡颗粒细胞的增殖、卵子的形成及发育、动物受精、胚胎发育、以及雄性动物生殖功能等一系列生殖过程,为进一步研究卵泡液外泌体对哺乳动物生殖功能的调控提供新思路。作者重点阐述了卵泡液外泌体分离鉴定的方法、主要成分以及调控哺乳动物生殖功能的分子机制的最新进展,为全面了解外泌体结构功能、生发凋亡和作用机制提供参考,为利用外泌体探索动物生殖理论和提高动物繁殖技术并将其应用于生产实际提供相关基础信息。

人类水通道蛋白在不同径线卵泡的黄素化颗粒细胞中的表达水平

【目的】探讨取卵日不同径线卵泡的黄素化颗粒细胞中各种水通道蛋白(AQPs)的mRNA表达水平。【方法】收集2022年3月25日至2022年9月23日在本中心行体外受精胚胎移植助孕的不孕患者取卵日的卵泡液共48例,根据卵泡径线分为小(<13 mm)、中(13~18 mm)、大(≥18 mm)卵泡群分开收集卵泡液提取颗粒细胞。经RNA定量后纳入22例进行分析,比较3组卵泡群的AQPs mRNA表达水平。根据促排卵方案将患者分为拮抗剂组和激动剂组,分析AQPs的表达差异。【结果】黄素化颗粒细胞中AQP2的mRNA水平随卵泡径线的增加而升高(linear trend P=0.004),其差异在大、小卵泡组间具有统计学差异(P=0.017);该差异在拮抗剂组内具有统计学意义(P=0.0496),在激动剂组中无统计学意义(P=0.108)。【结论】AQP2在黄素化颗粒细胞中的mRNA水平随着卵泡径线的增长而增加,其表达量与促排卵方案有关,提示AQP2在卵泡生长和卵泡液生成过程中可能起到一定作用,且其mRNA表达水平可能受到FSH和LH的调节。

人卵泡液游离DNA提取及定量检测方法的比较

目的:比较不同的DNA提取方法、实时荧光定量PCR(qPCR)的引物及荧光染料对人卵泡液游离核DNA(cf-nDNA)和游离线粒体DNA(cf-mtDNA)定量检测的影响。方法:收集2018年3月~10月在华中科技大学同济医学院生殖医学中心进行体外受精/卵胞浆内单精子显微注射技术(IVF/ICSI)的40例患者卵泡液样本,分别用4种不同的方法(方法一:BeaverBeads^(TM) Circulating DNA试剂盒、方法二:蛋白酶K(PK)法、方法三:Hieff^(■) qPCR SYBR^(■) Green Master Mix和方法四:KOD SYBR^(■) qPCR Mix)提取和纯化卵泡液中的游离DNA(cf-DNA)样本;用4种cf-nDNA引物(ALU115、B2MF1、GAPDH和β-globin)和4种cf-mtDNA引物(ND1-primer1、ND1-primer2、hmito3和hmito5)分别定量检测卵泡液cf-nDNA和cf-mtDNA水平;比较qPCR实验中SYBR Green化学染料和TaqMan探针两种方法在定量检测上的差异。结果:提取cf-nDNA的浓度按以下顺序排列:方法一>方法四>方法二>方法三(P<0.05),提取cf-mtDNA的浓度按以下顺序排列:方法一>方法四>方法三>方法二(P<0.05);样本中cf-nDNA的扩增浓度按照以下顺序排列:ALU115>GAPDH>β-globin>B2MF1(P<0.05),4种cf-nDNA引物无相关性(P>0.05)。cf-mtDNA的扩增浓度按以下顺序排列:ND1-primer1>ND1-primer2>hmito5>hmito3(P<0.05)。ND1-primer1和ND1-primer2有很强的相关性(r=0.517,P<0.05),hmito3和hmito5也有很强的相关性(r=0.989,P<0.05);在以β-globin和ND1-primer1为引物的qPCR实验中,TaqMan探针法检测的Ct值高于SYBR Green化学染料法(P<0.05)。结论:人卵泡液样本的提取方法、引物和荧光染料的选择均影响cf-nDNA和cf-mtDNA的定量,建议根据不同的研究目的选择合适的方法。

基于卵泡液miRNA探讨多囊卵巢综合征特点及发病机制

多囊卵巢综合征(PCOS)引起的卵泡发育、排卵障碍以及代谢紊乱等问题严重影响育龄期女性的月经及生殖功能,并可导致一系列病理变化。微RNA(miRNA)作为调控转录后基因表达的非编码RNA,也是目前PCOS领域的研究热点。PCOS患者卵泡液内miRNA表达具有一定特点,其中差异表达的miRNA参与调节卵巢颗粒细胞增殖与凋亡异常、氧化应激及高雄激素分泌等PCOS相关病理变化的基因表达,且其表达水平与胰岛素抵抗、高雄激素血症等PCOS合并症相关。因此,卵泡液内miRNA可能成为PCOS防治的新靶点及其诊断分型、预测治疗结局的生物标志物,具有重要的研究价值。

卵泡液中sRAGE、Vit D和TNF-α的含量与卵母细胞质量的相关性研究

目的探讨卵泡液(FF)中可溶性晚期糖基化终产物受体(sRAGE)、维生素D(Vit D)及肿瘤坏死因子-α(TNF-α)水平对卵母细胞质量的影响。方法纳入2021年6月至2022年8月于联勤保障部队第九○○医院生殖中心就诊行IVF-ET治疗的不孕女性(12例)的81个卵母细胞的FF样本,通过ELISA法检测单个FF中sRAGE、Vit D和TNF-α的含量。根据卵母细胞成熟情况、D3胚胎发育情况、囊胚发育情况进行分组,分析各组间卵泡直径、卵泡体积、FF中的sRAGE、Vit D和TNF-α的含量差异;进一步分析sRAGE、Vit D和TNF-α三者之间的相关性及其与临床指标的相关性。结果成熟卵母细胞组中卵泡的直径、体积、FF的sRAGE和Vit D水平显著高于不成熟卵母细胞组(P均<0.05),优质胚胎组、优质囊胚组中FF的sRAGE、Vit D水平均显著高于非优质胚胎组(P均<0.05);FF中sRAGE、Vit D水平与卵母细胞成熟度、胚胎发育情况等指标均存在正相关关系(P均<0.05)。成熟卵母细胞组、优质胚胎组和优质囊胚组中FF的TNF-α水平显著低于不成熟卵母细胞组、非优质胚胎组和非优质囊胚组(P均<0.05),FF中TNF-α水平与卵母细胞成熟度、胚胎发育情况、囊胚发育情况等指标均有负相关关系(P均<0.05)。FF中sRAGE与Vit D水平显著正相关(P<0.001);FF中sRAGE、Vit D水平与TNF-α水平显著负相关(P<0.001)。结论不孕患者FF中sRAGE、Vit D及TNF-α水平可能影响卵母细胞质量,将其作为胚胎筛选指标,并结合胚胎形态学评分,可能提高对胚胎着床能力判断的准确性,为临床不孕患者的诊疗提供新思路。

肥胖型PCOS患者卵泡液中同型半胱氨酸浓度对胚胎质量的影响

目的探讨肥胖型多囊卵巢综合征(PCOS)患者卵泡液中同型半胱氨酸(Hcy)浓度对胚胎质量的影响。方法选取2021年1月至2022年7月于运城市中心医院生殖医学科行体外受精助孕的137例PCOS患者为研究对象,将体质量指数(BMI)≥25 kg/m^(2)的患者纳入肥胖型PCOS组(n=76),BMI<25 kg/m^(2)的患者纳入非肥胖型PCOS组(n=61)。所有患者均采用拮抗剂方案促排卵,取卵前1日收集患者空腹静脉血清,取卵日留取第1管无血液和其他污染的卵泡液,采用酶联免疫吸附试验法检测两组患者卵泡液及血清中Hcy含量。比较两组患者的基础资料、获卵数、胚胎发育情况、卵泡液及血清Hcy水平;又根据卵泡液Hcy水平不同进行亚组分析,比较不同Hcy水平各组的胚胎发育情况。结果肥胖型PCOS组BMI、不孕年限均显著高于非肥胖型PCOS组,基础E2水平显著低于非肥胖型PCOS组(P<0.05);肥胖型PCOS组受精率、优质胚胎率均显著低于非肥胖型PCOS组(P<0.05)。肥胖型PCOS组患者卵泡液中Hcy水平显著高于同组患者血清中Hcy水平(P<0.05),亦显著高于非肥胖型PCOS组卵泡液中Hcy水平(P<0.05),而非肥胖型PCOS组患者卵泡液Hcy水平与同组患者血清Hcy水平无显著性差异(P>0.05)。又根据卵泡液Hcy水平不同利用四分位数将两组PCOS患者分别分为高、中、低3个浓度组。非肥胖型PCOS患者高浓度组优质胚胎数、优质胚胎率均显著低于中、低浓度组(P<0.05);肥胖型PCOS患者高浓度组的受精数、受精率、2PN数均显著低于中浓度组(P<0.05),高浓度组的2PN率、优质胚胎数、优质胚胎率均显著低于中、低浓度组(P<0.05)。结论对肥胖型和非肥胖型PCOS患者来说,卵泡液适宜的Hcy浓度不同,但卵泡液高浓度Hcy都对其胚胎质量有负面影响。

输卵管性不孕患者卵泡液中活性氧、超氧化物歧化酶水平与胚胎质量关系

目的:探究卵泡液中活性氧(ROS)、超氧化物歧化酶(SOD)水平与输卵管因素不孕症患者胚胎质量低下的关系。方法:收集2019年4月-2022年1月于本院因输卵管因素导致不孕症行体外受精-胚胎移植(IVF)助孕患者85例临床资料,通过长效方案促排卵治疗,根据胚胎培养结果,以优质胚胎率为35%为临界值,>35%为A组(n=44例),<35%为B组(n=41例)。收集两组卵泡液,检测其ROS、SOD水平;抽取空腹静脉血,检测血清促卵泡激素、黄体生成素、雌激素与睾酮水平;阴道超声下穿刺取卵,计算获卵数,观察卵子成熟率;体外受精12h后观察其受精率,72h后计算优质胚胎率。采用Pearson法分析ROS、SOD水平与激素水平、胚胎质量关系。结果:A组卵泡液ROS水平(0.81±0.25 ng/ml)低于B组(6.97±1.72 ng/ml),SOD水平(1.14±0.28 IU/ml)高于B组(5.34±1.92 IU/ml)(P<0.05);两组促卵泡激素、雌激素水平无差异(P>0.05),A组黄体生成素、睾酮水平低于B组(P<0.05)。两组获卵数无差异(P>0.05),A组卵子成熟率、受精率、优质胚胎率高于B组(P<0.05)。Pearson法相关性分析,与黄体生成素、睾酮ROS水平呈正相关,SOD水平呈负相关;与受精率、优质胚胎率呈ROS水平负相关,SOD水平呈正相关(均P<0.05)。结论:输卵管因素不孕症患者胚胎质量低下的原因可能为氧化应激,卵泡液中ROS、SOD水平与性激素、受精率、优质胚胎率相关。