Screening for colonization with methicillin resistant Staphylococcus aureas (MRSA) is a key aspect of infection control to limit the nosocomial spread of this organism. Current methods for the detection of MRSA in cli...Screening for colonization with methicillin resistant Staphylococcus aureas (MRSA) is a key aspect of infection control to limit the nosocomial spread of this organism. Current methods for the detection of MRSA in clinical microbiology laboratories using conventional methods is time consuming. In this research we are trying to evaluate the use of real time PCR for the detection of MRSA. The PCR assay was evaluated in clinical isolates of MRSA (n = 45) and methicillin susceptible Staphylococcus aureas MSSA (n = 10). The diagnostic values of the assay showed high sensitivity and specificity. This real-time PCR assay proved to be a fast, sensitive and specific tool for MRSA detection in a routine microbiological laboratory. Real-time PCR now is available in all laboratories so its use in identification of MRSA will help in shortening the period for MRSA identification and will help in the success of infection control programs in hospitals.展开更多
Single-nucleotide polymorphisms(SNPs)are the third generation of genetic markers,having been refined from the first generation of restriction fragment length polymorphisms and the second generation of microsatellite p...Single-nucleotide polymorphisms(SNPs)are the third generation of genetic markers,having been refined from the first generation of restriction fragment length polymorphisms and the second generation of microsatellite polymorphisms.SNPs represent a focal point of current studies of Staphylococcus aureus.On one hand,this review aims to summarize common methodologies for detecting SNPs.These methods have typically included DNA genome sequencing methods and PCR-based detection methods.Alternative methods,such as mass spectrometry,denaturing high-performance liquid chromatography,SNaPshot,and SNP array have also been employed for SNP analysis.On the other hand,we enumerate a series of applications of SNP analysis in investigations of Staphylococcus aureus.SNP analysis can be applied to investigate epidemiological outbreaks and transmission of Staphylococcus aureus infections,the transmission and evolution of antimicrobial resistance genes in Staphylococcus aureus isolates,interactions of Staphylococcus aureus with other bacteria,and the links between Staphylococcus aureus in humans and livestock.展开更多
文摘Screening for colonization with methicillin resistant Staphylococcus aureas (MRSA) is a key aspect of infection control to limit the nosocomial spread of this organism. Current methods for the detection of MRSA in clinical microbiology laboratories using conventional methods is time consuming. In this research we are trying to evaluate the use of real time PCR for the detection of MRSA. The PCR assay was evaluated in clinical isolates of MRSA (n = 45) and methicillin susceptible Staphylococcus aureas MSSA (n = 10). The diagnostic values of the assay showed high sensitivity and specificity. This real-time PCR assay proved to be a fast, sensitive and specific tool for MRSA detection in a routine microbiological laboratory. Real-time PCR now is available in all laboratories so its use in identification of MRSA will help in shortening the period for MRSA identification and will help in the success of infection control programs in hospitals.
文摘目的:建立同步速测食品中沙门氏菌、金黄色葡萄球菌、单核细胞增生性李斯特菌的多重聚合酶链式反应(polymerase chain reaction,PCR)方法。方法:利用基因组比对法寻找3种致病菌的特异性序列——沙门氏菌的invA基因、金黄色葡萄球菌的nuc基因和单增李斯特菌的prs基因,运用Primer Premier 5.0分别设计3对片段大小不同的特异性引物;通过优化反应体系,建立3种致病菌的多重PCR检测体系。结果:建立的多重PCR方法灵敏度测试结果分别为7.6、3.8、5.1pg/μL,在此灵敏度下可以扩增出全部特异性引物条带,验证性实验结果出现相应的目的条带且未发生交叉影响。结论:初步建立能同步、简便、快速、灵敏地检测食品中沙门菌、金黄色葡萄球菌和单核细胞增生性李斯特菌的三重PCR方法。
文摘Single-nucleotide polymorphisms(SNPs)are the third generation of genetic markers,having been refined from the first generation of restriction fragment length polymorphisms and the second generation of microsatellite polymorphisms.SNPs represent a focal point of current studies of Staphylococcus aureus.On one hand,this review aims to summarize common methodologies for detecting SNPs.These methods have typically included DNA genome sequencing methods and PCR-based detection methods.Alternative methods,such as mass spectrometry,denaturing high-performance liquid chromatography,SNaPshot,and SNP array have also been employed for SNP analysis.On the other hand,we enumerate a series of applications of SNP analysis in investigations of Staphylococcus aureus.SNP analysis can be applied to investigate epidemiological outbreaks and transmission of Staphylococcus aureus infections,the transmission and evolution of antimicrobial resistance genes in Staphylococcus aureus isolates,interactions of Staphylococcus aureus with other bacteria,and the links between Staphylococcus aureus in humans and livestock.
