An effective method for the trace analysis of indoxacarb residue in foodstuffs of plant and animal origin [grapefruit, ginger, fresh soybean, bamboo shoot, qing-gen-cai （cruciferous vegetable）, chicken, fish, and po...An effective method for the trace analysis of indoxacarb residue in foodstuffs of plant and animal origin [grapefruit, ginger, fresh soybean, bamboo shoot, qing-gen-cai （cruciferous vegetable）, chicken, fish, and pork] was developed using gas chromatography （GC-ECD） and liquid chromatography tandem mass spectrometry （LC-MS/MS）. Samples were extracted using acetone and n-hexane mixed solvent （1：2, v/v） and then purified using solid-phase extraction （SPE） columns. The extracts were analyzed using GC-ECD and LC-MS/MS. The multiple reaction monitoring （MRM） scheme used involved transitions of the precursor ions to selected two product ions in which one pair for identification was m/z 529 → 293 and another pair for quantification was m/z 529 → 249. The detection limits （LODs） of the method were 0.0015 and 0.0006 mg kg^-1, and the quantification limits （LOQs） were 0.005 and 0.002 mg kg^-1 for GC-ECD and LC-MS/MS, respectively. The relative standard deviations （RSDs） of recovery for indoxacarb were lower than 15% in 10 types of agro-products. Ten repetitive determinations of recovery achieved good reproducibility for indoxacarb and the recovery ranged from 72.08 to 113.74%. The proposed procedure was applied to the analysis of several real samples of different origin from Fujian Province, China, and 299 samples were screened for indoxacarb residue, of which 5 positive samples were found.展开更多
Plant antimicrobial peptides are a very large family of antimicrobial peptides,which have strong resistance to various pathogenic microorganisms,especially fungi.With the increasing use of antibiotics,the problems cau...Plant antimicrobial peptides are a very large family of antimicrobial peptides,which have strong resistance to various pathogenic microorganisms,especially fungi.With the increasing use of antibiotics,the problems caused by antibiotics,including antibiotic residues and pathogen resistance,are becoming more and more prominent.The research on antimicrobial peptides as new antibiotic substitutes is also a hot spot.This article introduces the action sites and antibacterial mechanisms of several plant antimicrobial peptides,as well as the application of plant antimicrobial peptides in the fields of medicine,agriculture,and food preservation.展开更多
利用超高效液相色谱—飞行时间质谱仪筛查动物源性食品中165种兽药残留。采用含0.2%甲酸的乙腈∶水(8∶2)作为提取剂,Oasis PRIME HLB通过式净化,以甲醇(含0.1%甲酸)和2.0 mmol甲酸铵溶液(含0.1%甲酸)为流动相梯度洗脱,采用Waters Acqui...利用超高效液相色谱—飞行时间质谱仪筛查动物源性食品中165种兽药残留。采用含0.2%甲酸的乙腈∶水(8∶2)作为提取剂,Oasis PRIME HLB通过式净化,以甲醇(含0.1%甲酸)和2.0 mmol甲酸铵溶液(含0.1%甲酸)为流动相梯度洗脱,采用Waters Acquity HSS T3 UPLC色谱柱(100 mm×2.1 mm,1.7μm)分离,电喷雾离子源MSE正离子模式下进行扫描,根据化合物的保留时间、母离子和子离子碎片信息鉴定165种化合物。在猪肉、鱼肉、鸡肉、鸡蛋4种基质165种化合物的平均回收率在53.15%~132.35%。该方法前处理方法简单,能在短时间内实现大批量样品的处理和筛查定性,筛查结果准确可靠,可应用于实际样品的检测。展开更多
基金grants from Fujian Pro- vincial Department of Science and Technology (2006F3002)Xiamen Science and Technology Bureau (3502Z20072003)China Postdoctoral Programme (20060390291)
文摘An effective method for the trace analysis of indoxacarb residue in foodstuffs of plant and animal origin [grapefruit, ginger, fresh soybean, bamboo shoot, qing-gen-cai （cruciferous vegetable）, chicken, fish, and pork] was developed using gas chromatography （GC-ECD） and liquid chromatography tandem mass spectrometry （LC-MS/MS）. Samples were extracted using acetone and n-hexane mixed solvent （1：2, v/v） and then purified using solid-phase extraction （SPE） columns. The extracts were analyzed using GC-ECD and LC-MS/MS. The multiple reaction monitoring （MRM） scheme used involved transitions of the precursor ions to selected two product ions in which one pair for identification was m/z 529 → 293 and another pair for quantification was m/z 529 → 249. The detection limits （LODs） of the method were 0.0015 and 0.0006 mg kg^-1, and the quantification limits （LOQs） were 0.005 and 0.002 mg kg^-1 for GC-ECD and LC-MS/MS, respectively. The relative standard deviations （RSDs） of recovery for indoxacarb were lower than 15% in 10 types of agro-products. Ten repetitive determinations of recovery achieved good reproducibility for indoxacarb and the recovery ranged from 72.08 to 113.74%. The proposed procedure was applied to the analysis of several real samples of different origin from Fujian Province, China, and 299 samples were screened for indoxacarb residue, of which 5 positive samples were found.
文摘Plant antimicrobial peptides are a very large family of antimicrobial peptides,which have strong resistance to various pathogenic microorganisms,especially fungi.With the increasing use of antibiotics,the problems caused by antibiotics,including antibiotic residues and pathogen resistance,are becoming more and more prominent.The research on antimicrobial peptides as new antibiotic substitutes is also a hot spot.This article introduces the action sites and antibacterial mechanisms of several plant antimicrobial peptides,as well as the application of plant antimicrobial peptides in the fields of medicine,agriculture,and food preservation.
文摘利用超高效液相色谱—飞行时间质谱仪筛查动物源性食品中165种兽药残留。采用含0.2%甲酸的乙腈∶水(8∶2)作为提取剂,Oasis PRIME HLB通过式净化,以甲醇(含0.1%甲酸)和2.0 mmol甲酸铵溶液(含0.1%甲酸)为流动相梯度洗脱,采用Waters Acquity HSS T3 UPLC色谱柱(100 mm×2.1 mm,1.7μm)分离,电喷雾离子源MSE正离子模式下进行扫描,根据化合物的保留时间、母离子和子离子碎片信息鉴定165种化合物。在猪肉、鱼肉、鸡肉、鸡蛋4种基质165种化合物的平均回收率在53.15%~132.35%。该方法前处理方法简单,能在短时间内实现大批量样品的处理和筛查定性,筛查结果准确可靠,可应用于实际样品的检测。
