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A Study on Negative Feedback in English Extensive Reading Course
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作者 LIU Jun 《Sino-US English Teaching》 2024年第9期419-423,共5页
Negative feedback serves as a means of correcting and rectifying students’utterances.While content feedback is commonly used in extensive reading course,indirect feedback dominates in written assignments,providing st... Negative feedback serves as a means of correcting and rectifying students’utterances.While content feedback is commonly used in extensive reading course,indirect feedback dominates in written assignments,providing students with opportunities for second thoughts and self-correction.For oral limited-response and open-ended questions,recasts are predominantly used to give students more time to reflect and reduce teacher talk,thereby mitigating anxiety and enhancing learning motivation. 展开更多
关键词 negative feedback indirect feedback RECASTS
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Robust Digital Predistortion for LTE/5G Power Amplifiers Utilizing Negative Feedback Iteration 被引量:1
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作者 LIU Xin CHEN Wenhua +1 位作者 WANG Dehan NING Dongfang 《ZTE Communications》 2020年第3期49-56,共8页
A robust digital predistortion(DPD)technique utilizing negative feedback iteration is introduced for linearizing power amplifiers(PAs)in long term evolution(LTE)/5G systems.Different from the conventional direct learn... A robust digital predistortion(DPD)technique utilizing negative feedback iteration is introduced for linearizing power amplifiers(PAs)in long term evolution(LTE)/5G systems.Different from the conventional direct learning and indirect learning structure,the proposed DPD suggests a two-step method to identify the predistortion.Firstly,a negative feedback based iteration is used to estimate the optimal DPD signal.Then the corresponding DPD parameters are extracted by forward modeling with the input signal and optimal DPD signal.The iteration can be applied to both single-band and dual-band PAs,which will achieve superior linear performance than the conventional direct learning DPD while having a relatively low computational complexity.The measurement is carried out on a broadband Doherty PA(DPA)with a 200 MHz bandwidth LTE signal at 2.1 GHz,and on a 5G DPA with two 10 MHz LTE signals at 3.4/3.6 GHz for validation in dual-band scenarios. 展开更多
关键词 5G digital predistortion power amplifiers negative feedback iteration
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Alterative wire-feed system based on arc voltage negative feedback
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作者 傅强 薛松柏 《China Welding》 EI CAS 2012年第2期38-42,共5页
Waveform control method was commonly adopted to reduce the spatter of CO2 arc welding and improve the weld formation. It certainly would reduce the self-regulation ability of arc due to the adoption of segmented const... Waveform control method was commonly adopted to reduce the spatter of CO2 arc welding and improve the weld formation. It certainly would reduce the self-regulation ability of arc due to the adoption of segmented constant current control which especially led to arc" blowout with the abrupt change oat" arc" length or downward welding. Therefore alterative wire-feed system based on arc voltage negative feedback was put forward to improve the .stability of arc" length in this paper. Double closed-loop and double fuzzy P1 regulation were adopted in this system. Fuzzy control of induced voltage was adopted in the inner-loop which improved the stability and fast response of wire-feed system. Fuzz)" control of arc" voltage negative feedback was used in the outer-loop whose output .served as the input of negative feedback regulation of inner-loop induced voltage. This method could remain arc" length and weld penetration unchan, ged on the basils of reducing spatter and improving formation and it was proved by downward welding tests. 展开更多
关键词 are voltage negative feedback CO2 are welding waveform control aherative wire-feed system
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Negative Feedback in the Polar Ice System
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作者 Peter Stallinga Igor Khmelinskii 《Atmospheric and Climate Sciences》 2017年第1期76-91,共16页
One of the ingredients of the anthropogenic global warming hypothesis is the existence of large positive feedback in the climate system. An example is polar ice that, once melted, turns into blacker water that will in... One of the ingredients of the anthropogenic global warming hypothesis is the existence of large positive feedback in the climate system. An example is polar ice that, once melted, turns into blacker water that will increase radiation absorption and this rein-forces the melting. This causes a run-away scenario with a point of no return. Here it is shown that the polar ice can also have negative feedback aspects, where a melting of polar ice will cause it to reappear. 展开更多
关键词 ICE ALBEDO negative feedback FRESNEL
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Analysis of positive feedback in the control of movement
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作者 Soroor Behbahani Amir Homayoun Jafari 《Journal of Biomedical Science and Engineering》 2009年第7期480-483,共4页
Over the past three decades, neurophysiologists studying the neural circuitry responsible for control of skeletal muscles have developed several different general theories of sensorimotor control. These have usually i... Over the past three decades, neurophysiologists studying the neural circuitry responsible for control of skeletal muscles have developed several different general theories of sensorimotor control. These have usually invoked one or more of the sources of proprioceptive signals (e.g. muscle spindle and Golgi tendon organ afferents) in positive or negative feed-back loops to the homonymous alpha motoneurones. In this paper we consider to analyze the role of posi-tive feedback in combination of negative feedback due to important role of them in stabilizing the neu-romuscular system. 展开更多
关键词 Positive feedback negative feedback HILL MODEL REFLEX MODEL
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Solid-state wideband GaN HEMT power amplifier with a novel negative feedback structure
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作者 程知群 贾民仕 +1 位作者 栾雅 连心想 《Journal of Semiconductors》 EI CAS CSCD 2014年第12期79-82,共4页
The design and fabrication of an ultra-broadband power amplifier based on a GaN HEMT, which operates in the frequency range from 3 to 8 GHz, is presented in this paper. A TGF2023-02 GaN HEMT chip from Tri Quint is ado... The design and fabrication of an ultra-broadband power amplifier based on a GaN HEMT, which operates in the frequency range from 3 to 8 GHz, is presented in this paper. A TGF2023-02 GaN HEMT chip from Tri Quint is adopted and modeled. A novel negative feedback structure is applied in the circuit. The measured results show that the amplifier module has a wide range frequency response that is almost consistent with those of simulation at frequencies from 3 to 6.5 GHz. The measured power gain is greater than 7 dB between 3 and 6.5 GHz.The saturated output power is 38.5 dBm under DC bias of Vds =D28 V, Vgs D =-3:5 V at the frequency of 5.5 GHz. 展开更多
关键词 GaN HEMT MODELING power amplifier WIDEBAND negative feedback
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Realization of Fully Differential Fourth-Order Bessel Filter with Accurate Group Delay 被引量:1
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作者 江金光 何怡刚 吴杰 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2003年第9期927-932,共6页
A fully differential R-MOSFET-C fourth-order Bessel active lowpass filter employing fully differential operational amplifier,passive resistors,and current-steering MOS transistors as a variable resistor is proposed.T... A fully differential R-MOSFET-C fourth-order Bessel active lowpass filter employing fully differential operational amplifier,passive resistors,and current-steering MOS transistors as a variable resistor is proposed.This proposed implementation relies on the tunability of current-steering MOS transistors operating in the triode region counteracting the concert deviation of resistor in the integrated circuit manufacturing technology in orde r that the group delay of Bessel active filter could be designed accurately.The amplifier is not only with voltage common-mode negative feedback,but also with current common-mode negative feedback,which will benefit for the stability of its D C operating point.0.75μs group delay fourth-order Bessel lowpass filter,whic h is synthesized according to passive doubly terminated RLC prototype lowpass filter,demonstrates better than -65dB THD using 100kHz,2.5V pp signal in Taiwan UMC 2P2M(2-poly,2-metal)5.0V,0.5μm CMOS technology. 展开更多
关键词 fully differential voltage and curr ent common-mode negative feedback frequency tunable R-MOSFET-C filter Bessel filter
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RESEARCH ON NONLINEAR DISTORTION IN AMPLIFIERS 被引量:2
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作者 王成华 包骅 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2000年第2期182-187,共6页
Transistors are nonlinear devices, which can produce nonlinear distortion in amplifier while amplifying signals. For weak nonlinear distortion, the expressions of total harmonic distortion (THD), the second order in... Transistors are nonlinear devices, which can produce nonlinear distortion in amplifier while amplifying signals. For weak nonlinear distortion, the expressions of total harmonic distortion (THD), the second order intermodulation distortion(IM2 ), the third order intermodulation distortion(IM 3) and intercept point(IP 3) are deduced. With the aid of software Multisim, we simulate transistor common emitter amplifier, transistor common emitter amplifier with resistor in emitter, differential amplifier and differential amplifier with resistor between emitters. The simulational results and theoretical analyses are almost the same. 展开更多
关键词 nonlinear distortion intermodulation distortion intercept point negative feedback differential amplifier
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反馈类型的判断方法
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作者 郝宁生 陆英 《韶关大学学报》 1999年第4期87-91,共5页
反馈类型的判断和引入是电路分析和设计的重点和难点,本文就各种反馈类型的判断方法进行了详细的阐述。
关键词 负反馈 电压反馈 电流反馈 串联反馈 并联反馈 电子技术
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Temporal Structures of the North Atlantic Oscillation and Its Impact on the Regional Climate Variability 被引量:7
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作者 黄建平 季明霞 +1 位作者 Kaz HIGUCHI Amir SHABBAR 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2006年第1期23-32,共10页
In this study, the temporal structure of the variation of North Atlantic Oscillation (NAO) and its impact on regional climate variability are analyzed using various datasets. The results show that blocking formation... In this study, the temporal structure of the variation of North Atlantic Oscillation (NAO) and its impact on regional climate variability are analyzed using various datasets. The results show that blocking formations in the Atlantic region are sensitive to the phase of the NAO. Sixty-seven percent more winter blocking days are observed during the negative phase compared to the positive phase of the NAO. The average length of blocking during the negative phase is about 11 days, which is nearly twice as long as the 6-day length observed during the positive phase of the NAO. The NAO-related differences in blocking frequency and persistence are associated with changes in the distribution of the surface air temperature anomaly, which, to a large extent, is determined by the phase of the NAO. The distribution of regional cloud amount is also sensitive to the phase of the NAO. For the negative phase, the cloud amounts are significant, positive anomalies in the convective zone in the Tropics and much less cloudiness in the mid latitudes. But for the positive phase of the NAO, the cloud amount is much higher in the mid-latitude storm track region. In the whole Atlantic region, the cloud amount shows a decrease with the increase of surface air temperature. These results suggest that there may be a negative feedback between the cloud amount and the surface air t.emperature in the Atlantic region. 展开更多
关键词 North Atlantic Oscillation atmospheric blocking CLOUD negative feedback
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Dynamics and Mechanism of A Quorum Sensing Network Regulated by Small RNAs in Vibrio Harveyi 被引量:1
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作者 SHEN Jian-Wei 《Communications in Theoretical Physics》 SCIE CAS CSCD 2011年第3期465-472,共8页
Bacterial quorum sensing (QS) has attracted much interests and it is an important process of cell communication. Recently, Bassler et al. studied the phenomena of QS regulated by small RNAs and the experimental data... Bacterial quorum sensing (QS) has attracted much interests and it is an important process of cell communication. Recently, Bassler et al. studied the phenomena of QS regulated by small RNAs and the experimental data showed that smafl RNAs played important role in the QS of Vibrio harveyi and it can permit the fine-tuning of gene regulation and mmntenance of homeostasis. According to Michaelis-Menten kinetics and mass action law in this paper, we construct a mathematical model to investigate the mechanism induced QS by coexist of small RNA and signal molecular (AI) and show that there are periodic oscillation when the time delay and Hill coefficient exceed a critical value and the periodic oscillation produces the change of concentration and induces QS. These results are fit to the experimental results. In the meanwhile, we also get some theoretical value of Hopf Bifurcation on time deday. In addition, we also find this network is robust against noise. 展开更多
关键词 quorum sensing genetic network OSCILLATION small RNA BIFURCATION negative feedback loop
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Design of a 12-Gbit/s CMOS DNFFCG differential transimpedance amplifier 被引量:1
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作者 Fan Chen Wang Rong Wang Zhigong 《Journal of Southeast University(English Edition)》 EI CAS 2018年第1期1-5,共5页
A 12-Gbit/s low-power,wide-bandwidh CMOS(complementary metal oxide semiconductor)dual negative feedback feed-forward common gate(DNFFCG)differential trans-impedance amplifier(TIA)is presented for the veryshort-reach(V... A 12-Gbit/s low-power,wide-bandwidh CMOS(complementary metal oxide semiconductor)dual negative feedback feed-forward common gate(DNFFCG)differential trans-impedance amplifier(TIA)is presented for the veryshort-reach(VSR)optoelectronic integrated circuit(OEIC)receiver.The dominant pole of the input node is shifted up to a high frequency,and thus the bandwidth of the CMOS DNFFCG TIA is improved.Besides,two negative feedback loops are used to reduce the input impedance and further increase the bandwidth.The proposed TIA was fabricated using TSMC 0.18 jxm CMOS technology.The whole circuit has a compact chip area,the core area of which is only 0.003 6 mm2.The power consumption is 14.6 mW excluding 2-stage differential buffers.The test results indicate that the 3 dB bandwidth of 9 GHz is achieved with a 1 8 V supply voltage and its trans-impedance gain is 49.2 dBH.The measured average equivalent input noise current density is 28.1 pA H z12.Under the same process conditions,the DNFFCG has better gain bandwidth product compared with those in the published papers. 展开更多
关键词 very-short-reach optoelectronic integrated circuit negative feedback feed-forward common gate transimpedance gain
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Cognitive inflexibility in Japanese adolescents and adults with autism spectrum disorders 被引量:1
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作者 Yuka Yasuda Ryota Hashimoto +5 位作者 Kazutaka Ohi Hidenaga Yamamori Michiko Fujimoto Satomi Umeda-Yano Haruo Fujino Masatoshi Takeda 《World Journal of Psychiatry》 SCIE 2014年第2期42-48,共7页
AIM: To investigate executive function in Japanese adolescents and adults with autism spectrum disorders(ASD) compared to Japanese controls.METHODS: Thirty-three individuals with ASD and 33 controls participated. The ... AIM: To investigate executive function in Japanese adolescents and adults with autism spectrum disorders(ASD) compared to Japanese controls.METHODS: Thirty-three individuals with ASD and 33 controls participated. The ASD and control groups' demographic variables were matched for gender(male/female: 20/13 vs 20/13), age(26.1 ± 11.5 vs 26.8 ± 9.6), years of education(13.2 ± 2.9 vs 14.2 ± 1.9), full-scale intelligence quotient(IQ)(103.0 ± 16.7 vs 103.7 ± 14.7), performance IQ(96.2 ± 16.1 vs 97.8 ± 15.0), and verbal IQ(107.9 ± 16.3 vs 107.7 ± 14.4). Participants performed the Wisconsin Card Sorting Test(WCST), which assesses the executive processes involved in problem solving and cognitive flexibility, and the Continuous Performance Test(CPT), which assesses attention and impulsivity. Symptoms were assessed by the Autism-Spectrum Quotient Japanese version(AQ-J). First, we compared the scores of the WCST between the groups using a Mann-Whitney U-test and conducted an analysis of covariance for the variables with the scores of category archives and CPT scores as covariates. Second, we analyzed the correlation between the scores of the WCST and the AQ-J in the ASD group using Pearson's r.RESULTS: The total errors(TE) and the percentages of perseverative errors of the Milner type(%PEM) and Nelson type(%PEN) among the TE in the ASD group were significantly worse compared with the control group(ASD vs Control, respectively: TE: 16.0 ± 6.2 vs 12.6 ± 3.5, P = 0.012; %PEM: 11.7 ± 10.7 vs 6.6 ± 8.9, P = 0.037; %PEN: 20.1 ± 14.5 vs 8.7 ± 10.4, P = 0.0011). In contrast, no significant difference was observed between the two groups in the scores of categories achieved on the WCST or the CPT. An analysis of covariance revealed significant differences between the groups in the %PEN scores(P = 0.0062) but not in the TE or the %PEM scores. These results suggest that Japanese adolescents and adults with ASD have cognitive inflexibility. Furthermore, our results suggest that Japanese adolescents and adults with ASD may have difficulties using negative feedback because perseverative errors of the Nelson type indicate persistence in choosing the incorrect reaction. By contrast, there was no significant correlation between the WCST and AQ-J scores.CONCLUSION: We confirmed the presence of cognitive inflexibility in Japanese adolescents and adults with ASD. Our results also indicated that subjects with ASD may not use negative feedback effectively. 展开更多
关键词 EXECUTIVE function AUTISM spectrum DISORDERS Perseverative errors of NELSON type negative feedback WISCONSIN Card Sorting Test
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Athletes who train on unstable compared to stable surfaces exhibit unique postural control strategies in response to balance perturbations 被引量:1
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作者 D.S.Blaise Williams Ⅲ Nicholas G.Murray Douglas W.Powell 《Journal of Sport and Health Science》 SCIE 2016年第1期70-76,共7页
Background:Athletes have been shown to exhibit better balance compared to non-athletes(NON).However,few studies have investigated how the surface on which athletes train affects the strategies adopted to maintain bala... Background:Athletes have been shown to exhibit better balance compared to non-athletes(NON).However,few studies have investigated how the surface on which athletes train affects the strategies adopted to maintain balance.Two distinct athlete groups who experience different types of sport-specific balance training are stable surface athletes(SSA) such as basketball players and those who train on unstable surfaces(USA) such as surfers.The purpose of this study was to investigate the effects of training surface on dynamic balance in athletes compared to NON.Methods:Eight NON,eight SSA,and eight USA performed five 20-s trials in each of five experimental conditions including a static condition and four dynamic conditions in which the support surface translated in the anteroposterior(AP) or mediolateral(ML) planes using positive or negative feedback paradigms.Approximate entropy(Ap En) and root mean square distance(RMS) of the center of pressure(Co P) were calculated for the AP and ML directions.Four 3 × 5(group × condition) repeated measures ANOVAs were used to determine significant effects of group and condition on variables of interest.Results:USA exhibited smaller Ap En values than SSA in the AP signals while no significant differences were observed in the ML Co P signals.Generally,the negative feedback conditions were associated with significantly greater RMS values than the positive feedback conditions.Conclusion:USA exhibit unique postural strategies compared to SSA.These unique strategies seemingly exhibit a direction-specific attribute and may be associated with divergent motor control strategies. 展开更多
关键词 Athletes Balance negative feedback Postural stability Training
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Research Status and Key Technologies of Geo-Electromagnetic Magnetic Induction Sensor
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作者 Yanzhang Wang Yunxia Wan +3 位作者 Chen Chen Hongyu Shi Jun Lin Defu Cheng 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2017年第4期38-49,共12页
According to different applications,the performance parameters of the several Geo-Electromagnetic Magnetic Induction Sensors( GEMISs) which are the most representative in the world are compared. After discussing the s... According to different applications,the performance parameters of the several Geo-Electromagnetic Magnetic Induction Sensors( GEMISs) which are the most representative in the world are compared. After discussing the several key technologies for improving the performance of GEMISs,the main conclusions are as follows: the application bandwidth can be expanded by adopting flux negative feedback( 1 m Hz-10 k Hz);"laminated"core structure is useful for suppressing eddy current loss; the effective permeability of the core has increased observably after adding flux concentrators to the two ends of the core; the chopper amplifier is a useful method to suppress 1/f noise for reducing the GEMIS's output noise; the indoor calibration scheme based on"long straight solenoid"proposed to obtain sensor sensitivity. Accordingly,the problem how to reduce the noise of GEMIS is urgent need to be solved,which will improve the performance of GEMIS. And there is plenty of work requires further study on parameters ' optimization of coil and core. In the future,miniaturization,intelligence and three-axis measurement are the development directions of GEMIS. 展开更多
关键词 GEMIS key technologies FLUX negative feedback “Laminated” corestructure FLUX CONCENTRATORS
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Tropical cyclone, as powerful natural self-oscillatory systems, and wind-energetic a way of struggle against it—The analysis of a cyclone from a position of the theory of vibrations
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作者 Kaganov William Ilich 《Natural Science》 2012年第7期508-515,共8页
It is considered three phases of development of a tropical cyclone: the origin, the steady-state regime and the termination, coinciding with three phases of self-oscillatory process: oscillation buid-up, dynamic balan... It is considered three phases of development of a tropical cyclone: the origin, the steady-state regime and the termination, coinciding with three phases of self-oscillatory process: oscillation buid-up, dynamic balance and failure of self-oscillations. The rough size of energy of a cyclone, as column of air rotating with high speed, at initial and final stages of development and comparison of the received sizes with nuclear and hydrogen bombs is calculated. The model of the electronic oscillator with negative active conductivity by the numerical solution of the equation of the Van-der-Pol is analyzed. Formal coincidence of transient in this model with increase of capacity of vortical air formation, that allows to make mnemonic model of a cyclone in the form of volume self-oscillatory system is shown. The numerical solution of the made equations allows to construct schedules of dynamic process for each of particles of the investigated environment. The general recipe on restraint of development of a cyclone by compulsory entering into it of attenuation is given. Realization of this recipe by means of group of platforms with powerful wind-electrogenerator and the fans and uses of a principle of negative feedback is shown. The results of laboratory experiment confirming idea, taken as a principle struggle against an arising air whirlwind, by transformation of a laminar stream in chaotic, turbulent are resulted. For fuller check offered a wind-energetic method of struggle against a cyclone carrying out of more scale experiment is necessary. 展开更多
关键词 Tropical CYCLONE Model SELF-OSCILLATIONS WIND Electrogenerator The Fan negative feedback
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A High Dynamic Range GMI Current Sensor
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作者 Aktham Asfour Jean-Paul Yonnet Manel Zidi 《Journal of Sensor Technology》 2012年第4期165-171,共7页
The design and performances of a high dynamic range DC-AC current sensor utilizing Giant Magneto-Impedance (GMI) are presented. The sensor is based on a GMI element with negative feedback. The sensing element is a 30 ... The design and performances of a high dynamic range DC-AC current sensor utilizing Giant Magneto-Impedance (GMI) are presented. The sensor is based on a GMI element with negative feedback. The sensing element is a 30 μm diameter GMI Co-based amorphous wire. It is curled to a toroidal core of 2 cm diameter. A bias magnetic field of about 650 A/m is applied to the GMI element to obtain an asymmetric GMI effect. A strong negative feedback is introduced to ensure linearity in a wide dynamic range. Analog conditioning electronics was fully developed. This includes a square wave oscillator based on an inverter trigger;a peak detector and a high gain amplifier with zero adjust. The GMI element is driven at a 3 MHz frequency and 5 mA peak-to-peak current. The closed-loop operations are investigated and the performances of the sensor are presented. DC current measurements are performed. The sensor exhibits good sensitivity and very good linearity, free from hysteresis, in a wide dynamic range of ±40 A. The sensitivity is about 0.24 V/A and the linearity error is about 0.02% of the full scale (FS). The hysteresis error is smaller than the measurement accuracy. AC current measurements using the developed sensor have also been successfully achieved. The sensor bandwidth in closed-loop was about 1.7 kHz. 展开更多
关键词 GMI CURRENT SENSOR negative feedback DC-AC CURRENT Measurements
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A Study on the Molecular Switch of Gene Expression of the Mouse Heart Nuclear DNA Fragments
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作者 袁明秀 JIN +6 位作者 Rui Niu Weiran Chen Zhijun Zhang Zhihong 《High Technology Letters》 EI CAS 2001年第4期1-7,共7页
It is observed by in situ stain that LDH (1 5) ...nNAD + can probably enter the nucleopore and can be bound bound specifically with the genes that encode them. During the in vitro expression, the dilution of heart nuc... It is observed by in situ stain that LDH (1 5) ...nNAD + can probably enter the nucleopore and can be bound bound specifically with the genes that encode them. During the in vitro expression, the dilution of heart nuclear DNA fragments could enhance the expression activity of LDH/DNA and the amount of expressed LDH (1 5) is in proportion to the amount of dissociable LDH (1 5) on the LDH/DNA. With the integration of 14C Leu to the proteins, it is also observed that the addition of LDH (1 5) ...nNAD + can suppress the in vitro expression activity of LDH/DNA. AFM observation shows that the regulation sequence at the both ends of active genes may be bound with such active factors as proteins encoded by the genes which probably is the main molecular switch of gene expression and regulation we have been always searching for. Our work shows the prospective application of the combination of AFM and isotope labeling in the research of biological reaction. 展开更多
关键词 Molecular switch of gene expression Positive and negative feedback regulation mechanism Gene expression and regulation AFM
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OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice 被引量:1
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作者 Kun Dong Fuqing Wu +21 位作者 Siqi Cheng Shuai Li Feng Zhang Xinxin Xing Xin Jin Sheng Luo Miao Feng Rong Miao Yanqi Chang Shuang Zhang Xiaoman You Peiran Wang Xin Zhang Cailin Lei Yulong Ren Shanshan Zhu Xiuping Guo Chuanyin Wu Dong-Lei Yang Qibing Lin Zhijun Cheng Jianmin Wan 《Molecular Plant》 SCIE CSCD 2024年第6期900-919,共20页
Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains unclear.I... Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains unclear.In this study,we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-of-function mutants that exhibit various abnormal spikelet structures.Interestingly,we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and OsJAZ7.We showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs,thereby promoting the ubiquitination of OsJAZ1 by the SCF^(OsCOI1a/OsCOI1b) complex and degradation via the 26S proteasome.This process ultimately releases OsMYC2,a core transcriptional regulator in the JA signaling pathway,to activate or repress JA-responsive genes,thereby maintaining normal plant(spikelet)development.However,in the osprmt6a-1 mutant,reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/OsCOI1b in the presence of JAs.As a result,OsJAZ1 proteins become more stable,repressing JA responses,thus causing the formation of abnormal spikelet structures.Moreover,we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner,thereby establishing a negative feedback loop to balance JA signaling.We further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high temperatures.Collectively,our study establishes a direct molecular link between arginine methylation and JA signaling in rice. 展开更多
关键词 protein arginine methylation jasmonate signaling negative feedback loop spikelet development adaptation to heat stress RICE
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Paradigms and Paradox in the Ethylene Signaling Pathway and Interaction Network 被引量:24
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作者 Qiong Zhao Hong-Wei Guo 《Molecular Plant》 SCIE CAS CSCD 2011年第4期626-634,共9页
Phytohormone ethylene plays pivotal roles in plant response to developmental and environmental signals. During the past few years, the emerging evidence has led us to a new understanding of the signaling mechanisms an... Phytohormone ethylene plays pivotal roles in plant response to developmental and environmental signals. During the past few years, the emerging evidence has led us to a new understanding of the signaling mechanisms and regulatory networks of the ethylene action. In this review, we focus on the major advances made in the past three years, particularly the findings leading to new paradigms and the observations under debate. With the recent demonstration of the regulation of the protein stability of numerous key signaling components including EIN3, ELL1, EIN2, ETR2, EBFI/EBF2, and ETPI/ETP2, we highlight proteasome-dependent protein degradation as an essential regulatory mechanism that is widely adopted in the ethylene signaling pathway. We also discuss the implication of the negative feedback mechanism in the ethylene signaling pathway in light of ethylene-induced ETR2 and EBF2 gene expression. Meanwhile, we summarize the controversy on the involvement of MKK9-MPK3/6 cascade in the ethylene signaling versus biosynthesis pathway, and discuss the possible role of this MAPK module in the ethylene action. Finally, we describe the complex interactions between ethylene and other signaling pathways including auxin, light, and plant innate immunity, and propose that EIN3/ EIL1 act as a convergence point in the ethylene-initiated signaling network. 展开更多
关键词 Ethylene signaling MAPK protein turnover negative feedback ETP1/2 interplay.
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