The fruit swelling effect of forchlorfenurun （CPPU） and fruit thinning effect of ethychlozate on Tainong 1 mango （Mangifera indica L. cv. ） were studied. Spraying 32 mg/L CPPU at 7 and 21 d post flowering for just...The fruit swelling effect of forchlorfenurun （CPPU） and fruit thinning effect of ethychlozate on Tainong 1 mango （Mangifera indica L. cv. ） were studied. Spraying 32 mg/L CPPU at 7 and 21 d post flowering for just once significantly improved the fruit size of mango, but had no significant impact on fruit quality and flavor. Spraying 200 mg/L ethychlozate at 7 d post flowering for just once significantly increased fruit drop rate and single fruit weight of mango; fruit grade was improved and total soluble solid increased to the maximum value, so ethychlozate had good fruit thinning effect.展开更多
Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea(CPPU),an exogenous cytokinin(CK)growth regulator,is the conventional method for inducing fruit set during melon(Cucumis melo L.)production;however,the mechanism by which ...Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea(CPPU),an exogenous cytokinin(CK)growth regulator,is the conventional method for inducing fruit set during melon(Cucumis melo L.)production;however,the mechanism by which CPPU induces fruit set is unclear.Through histological and morphological observations,fruit size was comparable between CPPU-induced fruits and normal pollinated fruits because CPPU-induced fruits had higher cell density but smaller cell size compared with normal pollinated fruits.CPPU promotes the accumulation of gibberellin(GA)and auxin and decreases the level of abscisic acid(ABA)during fruit set.Moreover,application of the GA inhibitor paclobutrazol(PAC)partially inhibits CPPU-induced fruit set.Transcriptome analysis revealed that CPPU-induced fruit set specifically induced the GA-related pathway,in which the key synthase encoding gibberellin 20-oxidase 1(CmGA20ox1)was specifically upregulated.Further study indicated that the two-component response regulator 2(CmRR2)of the cytokinin signaling pathway,which is highly expressed at fruit setting,positively regulates the expression of CmGA20ox1.Collectively,our study determined that CPPU-induced melon fruit set is dependent on GA biosynthesis,providing a theoretical basis for the creation of parthenocarpic melon germplasm.展开更多
Kiwifruit is an economically and nutritionally valuable fruit.Forchlorfenuron(CPPU)is widely used to improve fruit set and yield of kiwifruit.However,the mechanism through which CPPU regulates fruit enlargement remain...Kiwifruit is an economically and nutritionally valuable fruit.Forchlorfenuron(CPPU)is widely used to improve fruit set and yield of kiwifruit.However,the mechanism through which CPPU regulates fruit enlargement remains unclear.Physiological and RNA-seq data revealed that the greatest contribution of CPPU treatment to fruit growth occurred at 28 to 42 d after bloom(DAB).CPPU application accelerated sugar metabolism by increasing the content of sucrose through increasing the transcripts of genes such as SUSY,HK,and FK in sucrose metabolism.In the early stages of fruit development,up-regulation of the starch synthesis gene AGPase and the decomposition geneβ-AMY indicated the occurrence of starch turnover.After CPPU treatment,genes related to the synthesis and signal transduction of auxin and gibberellins were downregulated.During the early fruit developmental stage,the cytokinin content and the expression of signal transduction genes were increased.In addition,the expression of cyclin genes,related to cell division,was up-regulated within three weeks after CPPU treatment.This indicates that CPPU treatment promoted cell division during the fastest growth stage,which would lead to an increase in cell number and further increases in fruit size.展开更多
基金Supported by Natural Science Foundation of Hainan Province(314161)Special Fund for Agro-scientific Research in the Public Interest(201203092)
文摘The fruit swelling effect of forchlorfenurun （CPPU） and fruit thinning effect of ethychlozate on Tainong 1 mango （Mangifera indica L. cv. ） were studied. Spraying 32 mg/L CPPU at 7 and 21 d post flowering for just once significantly improved the fruit size of mango, but had no significant impact on fruit quality and flavor. Spraying 200 mg/L ethychlozate at 7 d post flowering for just once significantly increased fruit drop rate and single fruit weight of mango; fruit grade was improved and total soluble solid increased to the maximum value, so ethychlozate had good fruit thinning effect.
基金This work was supported by the National Natural Science Foundation of China(32225044 to Z.Z.,32130093 to Z.Z.,32102404 to S.C.,32002064 to Y.L.)This work was also supported by the Taishan Scholar Foundation of the People’s Government of Shandong Province and the Natural Science Foundation of Shandong Province(ZR2020QC157).
文摘Spraying N-(2-chloro-4-pyridyl)-N′-phenylurea(CPPU),an exogenous cytokinin(CK)growth regulator,is the conventional method for inducing fruit set during melon(Cucumis melo L.)production;however,the mechanism by which CPPU induces fruit set is unclear.Through histological and morphological observations,fruit size was comparable between CPPU-induced fruits and normal pollinated fruits because CPPU-induced fruits had higher cell density but smaller cell size compared with normal pollinated fruits.CPPU promotes the accumulation of gibberellin(GA)and auxin and decreases the level of abscisic acid(ABA)during fruit set.Moreover,application of the GA inhibitor paclobutrazol(PAC)partially inhibits CPPU-induced fruit set.Transcriptome analysis revealed that CPPU-induced fruit set specifically induced the GA-related pathway,in which the key synthase encoding gibberellin 20-oxidase 1(CmGA20ox1)was specifically upregulated.Further study indicated that the two-component response regulator 2(CmRR2)of the cytokinin signaling pathway,which is highly expressed at fruit setting,positively regulates the expression of CmGA20ox1.Collectively,our study determined that CPPU-induced melon fruit set is dependent on GA biosynthesis,providing a theoretical basis for the creation of parthenocarpic melon germplasm.
基金supported by the Program for the National Natural Science Foundation of China(No.31901970).
文摘Kiwifruit is an economically and nutritionally valuable fruit.Forchlorfenuron(CPPU)is widely used to improve fruit set and yield of kiwifruit.However,the mechanism through which CPPU regulates fruit enlargement remains unclear.Physiological and RNA-seq data revealed that the greatest contribution of CPPU treatment to fruit growth occurred at 28 to 42 d after bloom(DAB).CPPU application accelerated sugar metabolism by increasing the content of sucrose through increasing the transcripts of genes such as SUSY,HK,and FK in sucrose metabolism.In the early stages of fruit development,up-regulation of the starch synthesis gene AGPase and the decomposition geneβ-AMY indicated the occurrence of starch turnover.After CPPU treatment,genes related to the synthesis and signal transduction of auxin and gibberellins were downregulated.During the early fruit developmental stage,the cytokinin content and the expression of signal transduction genes were increased.In addition,the expression of cyclin genes,related to cell division,was up-regulated within three weeks after CPPU treatment.This indicates that CPPU treatment promoted cell division during the fastest growth stage,which would lead to an increase in cell number and further increases in fruit size.
