[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR produ...[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.展开更多
We studied the formation of heartwood in Ery- throphleumfordii Oliv., an endangered rosewood species, by cross-section analysis on 52 stumps aged 28-57 years and arbitrarily sampled from natural forests in South China...We studied the formation of heartwood in Ery- throphleumfordii Oliv., an endangered rosewood species, by cross-section analysis on 52 stumps aged 28-57 years and arbitrarily sampled from natural forests in South China. Width and area of heartwood were positively correlated with age and xylem width （XW）, and were independent of annual radial growth. The proportions of heartwood and sapwood areas were equal at about 40 years. The heartwood formation pro- cess was simulated by linear regression of age and XW.展开更多
Erythrophleum fordii Oliv.(Caesalpiniaceae)is a rosewood species naturally distributed in southeast Asia and south China,and the heartwood is commonly used for high-quality crafts and furniture.While there are differe...Erythrophleum fordii Oliv.(Caesalpiniaceae)is a rosewood species naturally distributed in southeast Asia and south China,and the heartwood is commonly used for high-quality crafts and furniture.While there are differences in heartwood development among single trees with distinct social status,the relationship between heartwood development and growth performance remains unclear.This information is essential to improve plantation management for high-yield heartwood production.Forty dominant,intermediate and suppressed trees were sampled from E.fordii plantations aged in 32-35 years in Pingxiang City,Guangxi,China.Stem analysis was carried out to determine horizontal and vertical variations of heartwood and sapwood.Number of annual rings,diameter and area of heartwood and sapwood as well as ratios of heartwood diameter and area at breast height were all significantly influenced by the social status of trees in stands(P<0.05).In these mid-aged plantations,E.fordii stems developed heartwood once the xylem diameter reached 5-10 cm,and then heartwood diameter and area increased with increasing xylem diameter.Heartwood ring numbers,diameter and area as well as their ratios decreased with increasing height,while sapwood ring numbers and diameter were relatively constant within the section where heartwood occurred.Heartwood and sapwood diameters were equal at heights of approximately 6-m for dominant,5-m for intermediate and 3-m for suppressed trees.Dominant trees differed considerably from intermediate and suppressed trees in heartwood volume,while heartwood volume ratios were all below 30%,and near 90%in the stem section below 8-m height regardless of social status.Relationship analysis showed that DBH was the most important factor influencing heartwood in even-aged stands.The findings provide evidence for crop tree selection,thinning regimes and reasonable management of plantations of E.fordii.展开更多
A new compound,(2′R)-(5-oxo-2,5-dihydrofuran-2-yl)methyl octanoate(1),together with 9 known ones(2-10)were isolated from the bioactive extract of the seed kernels from Vernicia fordii.Their structures were determined...A new compound,(2′R)-(5-oxo-2,5-dihydrofuran-2-yl)methyl octanoate(1),together with 9 known ones(2-10)were isolated from the bioactive extract of the seed kernels from Vernicia fordii.Their structures were determined by spectroscopic analyses,including Nuclear Magnetic Resonance(NMR)and High Resolution Electrospray Ionization Mass Spectroscopy(HRESI-MS).Their cytotoxicities against three human cancer cell lines(A549,HepG2 and Caco2 cells)and anti-neuroinflammatory effects were evaluated.Cytotoxicity assay revealed that compound 9 could inhibit the growth of HepG2 cells,while compound 6 had a moderate inhibitory effect against A549 cells.Further anti-neuroinflammatory activity assay showed that compound 10 exhibited moderate inhibitory effect on the release of NO in LPS-stimulated BV2 microglia cells.展开更多
In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compoun...In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compounds from plants as an alternative to synthetic organic chemical preservative.In Chinese folk,tung oil can be used as paint for wooden furniture to protect them from pests.This study aimed to evaluate the chemical compositions of raw and heated tung oil and their activity against termite.In choice bioassays,weight loss of wood treated with 5%raw or heated tung oil after 4 weeks was significantly less than that of the control group.In no-choice bioassays,there was a significant difference in termite survival and wood weight loss on raw and heated tung oil-treated wood.When tung oil-treatment concentrations increased to 5%,wood weight loss was less than 10%.There was no significant difference in termite survival and wood weight loss between raw and heated tung oil-treated wood.Survival of termites in both tung oil wood treatments was significantly lower than that in the starvation control after 4 weeks.Raw and heated tung oil significantly improved the resistance of pine wood to termites,and have the potential for the development of natural wood preservatives.展开更多
基金Supported by Research Fund of the Doctoral Program of Higher Education (200805720004)Scientific Research Foundation for Returned Scholars, Ministry of Education of China ([2009]1001)~~
文摘[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.
基金financially supported by the Special Research Program for Public-welfare Forestry(No.201204301-2)National Nonprofit Institute Research Grant of RITF(No.RITFKYYW2010-05)
文摘We studied the formation of heartwood in Ery- throphleumfordii Oliv., an endangered rosewood species, by cross-section analysis on 52 stumps aged 28-57 years and arbitrarily sampled from natural forests in South China. Width and area of heartwood were positively correlated with age and xylem width （XW）, and were independent of annual radial growth. The proportions of heartwood and sapwood areas were equal at about 40 years. The heartwood formation pro- cess was simulated by linear regression of age and XW.
基金This study is supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF(Grant No.CAFYBB2017MB019).
文摘Erythrophleum fordii Oliv.(Caesalpiniaceae)is a rosewood species naturally distributed in southeast Asia and south China,and the heartwood is commonly used for high-quality crafts and furniture.While there are differences in heartwood development among single trees with distinct social status,the relationship between heartwood development and growth performance remains unclear.This information is essential to improve plantation management for high-yield heartwood production.Forty dominant,intermediate and suppressed trees were sampled from E.fordii plantations aged in 32-35 years in Pingxiang City,Guangxi,China.Stem analysis was carried out to determine horizontal and vertical variations of heartwood and sapwood.Number of annual rings,diameter and area of heartwood and sapwood as well as ratios of heartwood diameter and area at breast height were all significantly influenced by the social status of trees in stands(P<0.05).In these mid-aged plantations,E.fordii stems developed heartwood once the xylem diameter reached 5-10 cm,and then heartwood diameter and area increased with increasing xylem diameter.Heartwood ring numbers,diameter and area as well as their ratios decreased with increasing height,while sapwood ring numbers and diameter were relatively constant within the section where heartwood occurred.Heartwood and sapwood diameters were equal at heights of approximately 6-m for dominant,5-m for intermediate and 3-m for suppressed trees.Dominant trees differed considerably from intermediate and suppressed trees in heartwood volume,while heartwood volume ratios were all below 30%,and near 90%in the stem section below 8-m height regardless of social status.Relationship analysis showed that DBH was the most important factor influencing heartwood in even-aged stands.The findings provide evidence for crop tree selection,thinning regimes and reasonable management of plantations of E.fordii.
基金financially supported by National Natural Science Foundation of China(Grant No.81872768,81673323,U1903122)Liaoning Revitalization Talents Program(XLYC1807118)Liaoning BaiQianWan Talents Program(2018)。
文摘A new compound,(2′R)-(5-oxo-2,5-dihydrofuran-2-yl)methyl octanoate(1),together with 9 known ones(2-10)were isolated from the bioactive extract of the seed kernels from Vernicia fordii.Their structures were determined by spectroscopic analyses,including Nuclear Magnetic Resonance(NMR)and High Resolution Electrospray Ionization Mass Spectroscopy(HRESI-MS).Their cytotoxicities against three human cancer cell lines(A549,HepG2 and Caco2 cells)and anti-neuroinflammatory effects were evaluated.Cytotoxicity assay revealed that compound 9 could inhibit the growth of HepG2 cells,while compound 6 had a moderate inhibitory effect against A549 cells.Further anti-neuroinflammatory activity assay showed that compound 10 exhibited moderate inhibitory effect on the release of NO in LPS-stimulated BV2 microglia cells.
基金This study was funded by College Students’Scientific Research Training Program of Zhejiang A&F University(No.113-2013200148)ZAFU-Institute of Termite Control of Yuhang cooperative project(2045200485,2045200529).
文摘In worldwide,the use of chemical pesticides to protect wood has been greatly restricted.In recent years,a large number of researchers devoted to the search for natural,safe and non-polluting bioactive chemical compounds from plants as an alternative to synthetic organic chemical preservative.In Chinese folk,tung oil can be used as paint for wooden furniture to protect them from pests.This study aimed to evaluate the chemical compositions of raw and heated tung oil and their activity against termite.In choice bioassays,weight loss of wood treated with 5%raw or heated tung oil after 4 weeks was significantly less than that of the control group.In no-choice bioassays,there was a significant difference in termite survival and wood weight loss on raw and heated tung oil-treated wood.When tung oil-treatment concentrations increased to 5%,wood weight loss was less than 10%.There was no significant difference in termite survival and wood weight loss between raw and heated tung oil-treated wood.Survival of termites in both tung oil wood treatments was significantly lower than that in the starvation control after 4 weeks.Raw and heated tung oil significantly improved the resistance of pine wood to termites,and have the potential for the development of natural wood preservatives.
