Screening was done for the isolation of effective lignin degraders from the forest soil samples, by providing lignin as a carbon source through the enrichment method, which leads to the isolation of 8 effective fungal...Screening was done for the isolation of effective lignin degraders from the forest soil samples, by providing lignin as a carbon source through the enrichment method, which leads to the isolation of 8 effective fungal isolates among 14 isolates. Submerged fermentation was done for the production of ligninolytic enzymes with the effective microorganisms by providing Guiaicol as a carbon source. The assay of laccase, lignin peroxidise activity and specific activity was done after the incubation intervals of 2, 4, 6, 7, 8, 10 and 12 days at 27±2℃ under shake culture condition. Partially purified protein content was estimated by using Lowry＇s method. Pleurotus sp. and Phanerochaetae chrysosporium are more effective at the 2nd and 7th days of incubation for the production of laccase and lignin peroxidases among the effective isolates.展开更多
Abstract： We assessed soil fungal diversity in the dry deciduous forest ofa Bhadra Wildlife Sanctuary of the Western Ghats （210.31 m a.s.1.; N 13044＇ and E75°37′）. Soil samples were collected by random mixed...Abstract： We assessed soil fungal diversity in the dry deciduous forest ofa Bhadra Wildlife Sanctuary of the Western Ghats （210.31 m a.s.1.; N 13044＇ and E75°37′）. Soil samples were collected by random mixed sampling during winter （November, 2008）, summer （March, 2009） and monsoon （August, 2009） seasons, and physico-chemical parameters were recorded. During winter, summer, and monsoon seasons, 49, 45 and 49 of fungal species belongs to 20, 18 and 19 of genera were isolated, respectively. Isolated soil fungi were mainly of the Mitosporic fungi, followed by Zygomycotina, Ascomycotina, Oomycotina and Coelomycetes. Indices of diversity, dominance and fisher alpha during winter, summer and monsoon seasons were 3.756, 3.638 and 3.738 （H）, 0.9737, 0.9694 and 0.9726 （I-D） and 18.84, 29.83 and 19.46 （a）, respectively. Spear- man＇s （r） correlation coefficient of fungal population with physicochemical parameters of soils showed significantly positive and negative correlations （p〈0.01） during winter, summer and monsoon seasons. Physico-chemical soil parameters played an important role in the occurrence, diversity, distribution, and relative abundance of fungal species in the tropical dry deciduous forest soil.展开更多
文摘Screening was done for the isolation of effective lignin degraders from the forest soil samples, by providing lignin as a carbon source through the enrichment method, which leads to the isolation of 8 effective fungal isolates among 14 isolates. Submerged fermentation was done for the production of ligninolytic enzymes with the effective microorganisms by providing Guiaicol as a carbon source. The assay of laccase, lignin peroxidise activity and specific activity was done after the incubation intervals of 2, 4, 6, 7, 8, 10 and 12 days at 27±2℃ under shake culture condition. Partially purified protein content was estimated by using Lowry＇s method. Pleurotus sp. and Phanerochaetae chrysosporium are more effective at the 2nd and 7th days of incubation for the production of laccase and lignin peroxidases among the effective isolates.
文摘Abstract： We assessed soil fungal diversity in the dry deciduous forest ofa Bhadra Wildlife Sanctuary of the Western Ghats （210.31 m a.s.1.; N 13044＇ and E75°37′）. Soil samples were collected by random mixed sampling during winter （November, 2008）, summer （March, 2009） and monsoon （August, 2009） seasons, and physico-chemical parameters were recorded. During winter, summer, and monsoon seasons, 49, 45 and 49 of fungal species belongs to 20, 18 and 19 of genera were isolated, respectively. Isolated soil fungi were mainly of the Mitosporic fungi, followed by Zygomycotina, Ascomycotina, Oomycotina and Coelomycetes. Indices of diversity, dominance and fisher alpha during winter, summer and monsoon seasons were 3.756, 3.638 and 3.738 （H）, 0.9737, 0.9694 and 0.9726 （I-D） and 18.84, 29.83 and 19.46 （a）, respectively. Spear- man＇s （r） correlation coefficient of fungal population with physicochemical parameters of soils showed significantly positive and negative correlations （p〈0.01） during winter, summer and monsoon seasons. Physico-chemical soil parameters played an important role in the occurrence, diversity, distribution, and relative abundance of fungal species in the tropical dry deciduous forest soil.
