Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ<sub>1-42</sub>-induced oligodendrocytes (OL) damage. ...Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ<sub>1-42</sub>-induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by Aβ<sub>1-42</sub>, an injury model was established by subjecting OLN-93 cells to Aβ<sub>1-42</sub> exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ<sub>1-42</sub>. The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate Aβ<sub>1-42</sub>-induced damage of OL.展开更多
Powdering/exfoliating of coatings and scratching galvanized steels and high strength steels （HSS）, are the main forms of surface damage in the forming of which result in increased die maintenance cost and scrap rate...Powdering/exfoliating of coatings and scratching galvanized steels and high strength steels （HSS）, are the main forms of surface damage in the forming of which result in increased die maintenance cost and scrap rate. In this study, a special rectangular box was developed to investigate the behavior and characteristics of surface damage in sheet metal forming （SMF） processes. U-channel forming tests were conducted to study the effect of tool hardness on surface damage in the forming of high strength steels and galvanized steels （hot-dip galvanized and galvannealed steels）. Experimental results indicate that sheet deformation mode influences the severity of surface damage in SMF and surface damage occurs easily at the regions where sheet specimen deforms under the action of compressive stress. Die corner is the position where surface damage initiates. For HSS sheet, surface damage is of major interest due to high forming pressure. The HSS and hot-dip galvanized steels show improved ability of damage-resistance with increased hardness of the forming tool. However, for galvannealed steel it is not the forming tool with the highest hardness value that performs best.展开更多
The use of ultra-high strength steels through sheet metal forming process offers a practical solution to the lightweight design of vehicles.However,sheet metal forming process not only produces desirable changes in ma...The use of ultra-high strength steels through sheet metal forming process offers a practical solution to the lightweight design of vehicles.However,sheet metal forming process not only produces desirable changes in material properties but also causes material damage that may adversely influence the service performance of the material formed.Thus,an investigation is conducted to experimentally quantify such influence for a commonly used steel（the 22MnB5 steel） based on the hot and cold forming processes.For each process,a number of samples are used to conduct a uniaxial tensile test to simulate the forming process.After that,some of the samples are trimmed into a standard shape and then uniaxially extended until fracture to simulate the service stage.Finally,a microstructure test is conducted to analyze the microdefects of the remaining samples.Based on the results of the first two tests,the effect of material damage on the service performance of 22MnB5 steel is analyzed.It is found that the material damages of both the hot and cold forming processes cause reductions in the service performance,such as the failure strain,the ultimate stress,the capacity of energy absorption and the ratio of residual strain.The reductions are generally lower and non-linear in the former process but higher and linear in the latter process.Additionally,it is found from the microstructure analysis that the difference in the reductions of the service performance of 22MnB5 by the two forming processes is driven by the difference in the micro damage mechanisms of the two processes.The findings of this research provide a useful reference in terms of the selection of sheet metal forming processes and the determination of forming parameters for 22MnB5.展开更多
This paper presents the application of anisotropic damage theory to the study of forming limit diagram of A12024T3 aluminum alloy sheet. In the prediction of limiting strains of the aluminum sheet structure, a finite ...This paper presents the application of anisotropic damage theory to the study of forming limit diagram of A12024T3 aluminum alloy sheet. In the prediction of limiting strains of the aluminum sheet structure, a finite element cell model has been constructed. The cell model consists of two phases, the aluminum alloy matrix and the intermetallic cluster. The material behavior of the aluminum alloy matrix is described with a fully coupled elasto-plastic damage constitutive equation. The intermetallic cluster is assumed to be elastic and brittle. By varying the stretching ratio, the limiting strains of the sheet under biaxial stretching have been predicted by using the necking criterion proposed. The prediction is in good agreement with the experimental findings. Moreover, the finite element cell model can provide information for understanding the microscopic damage mechanism of the aluminum alloy. Over-estimation of the limit strains may result if the effect of material damage is ignored in the sheet metal forming study.展开更多
Ductile damage often occurs during metal forming processes due to the large thermo-elasto （visco） plastic flow Iocalisation. This paper presents a numerical methodology, which aims to virtually improve any metal for...Ductile damage often occurs during metal forming processes due to the large thermo-elasto （visco） plastic flow Iocalisation. This paper presents a numerical methodology, which aims to virtually improve any metal forming processes. The methodology is based on elastoplastic constitutive equations accounting for nonlinear mixed isotropic and kinematic hardening strongly coupled with isotropic ductile damage. An adaptive remeshing scheme based on geometrical and physical error estimates including a kill element procedure is used. Some numerical results are presented to show the capability of the model to predict the damage initiation and growth during the metal forming processes.展开更多
The effects of forming damage are analyzed,which occur during hot stamping process,on the load-carrying capacity and failure mode of hot stamped beams.A damage-coupled pre-forming constitutive model was proposed,in wh...The effects of forming damage are analyzed,which occur during hot stamping process,on the load-carrying capacity and failure mode of hot stamped beams.A damage-coupled pre-forming constitutive model was proposed,in which the damage during hot stamping process was introduced into the service response.The constitutive model was applied into the three-point bending simulation of a hot stamped beam,and then the influences of forming damage on the load-carrying capacity and cracks propagation were investigated.The results show that the forming damage reduces the maximum load capacity of the hot stamped beam by 7.5%.It also causes the crack to occur earlier and promotes crack to propagate along the radial direction of the punch.展开更多
Low-permeability dense reservoirs,including micro-fractured reservoirs,are commonly characterized by high content of clay substances,high original water saturation,high sensitivity to invasive fluids,high capillary pr...Low-permeability dense reservoirs,including micro-fractured reservoirs,are commonly characterized by high content of clay substances,high original water saturation,high sensitivity to invasive fluids,high capillary pressure,complicated structure and anisotropic,high flow-resistance and micro pore throats etc,.Generally they also have lots of natural micro fractures,probably leading to stress sensibility.Main damaging factors in such reservoirs are water-sensibility and water-blocking caused by invasive fluids during drilling and production operations.Once damaged,formation permeability can rarely recovered.Numerous studies have shown that damaging extent of water-blocking ranges from 70% to 90%.Main damaging mechanisms and influencing factors of water-blocking were systematically analyzed.Also some feasible precaution or treating approaches of water-blocking were put forward.In a laboratory setting,a new multi-functional drilling fluid composed mainly of amphion polymer,sulfonation polymer,high effectively preventive water-blocking surfactants,ideal packing temporary bridging agents(TBA) and film-forming agents,etc.,were developed.New low-damage drilling fluids has many advantages,such as good rheological properties,excellent effectiveness of water-blocking prevention,good temporary plugging effect,low filtration and ultra-low permeability(API filtration≤5 mL,HTHP filtration≤10 mL,mud cake frictional coefficient≤0.14,permeability recovery>81%),can efficiently prevent or minimize damage,preserve natural formation and enhance comprehensive development-investment effect in TUHA Jurassic dense sandstone reservoir formation with low-permeability,the only one developing integrated condense gas field.Some references can be provided to similar reservoir formations.展开更多
The current study performed a finite element analysis of the strain localization behavior of a voided ductile material using a non-local plasticity formulation in which the yield strength depends on both an equivalent...The current study performed a finite element analysis of the strain localization behavior of a voided ductile material using a non-local plasticity formulation in which the yield strength depends on both an equivalent plastic strain measurement (hardening parameter) and Laplacian equivalent. The introduction of gradient terms to the yield function was found to play an important role in simulating the strain localization behavior of the voided ductile material. The effect of the mesh size and characteristic length on the strain localization were also investigated. An FEM simulation based on the proposed non-local plasticity revealed that the load-strain curves of the voided ductile material subjected to plane strain tension converged to one curve, regardless of the mesh size. In addition, the results using non-local plasticity also exhibited that the dependence of the deformation behavior of the material on the mesh size was much less sensitive than that with classical local plasticity and could be succe展开更多
文摘Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ<sub>1-42</sub>-induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by Aβ<sub>1-42</sub>, an injury model was established by subjecting OLN-93 cells to Aβ<sub>1-42</sub> exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ<sub>1-42</sub>. The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate Aβ<sub>1-42</sub>-induced damage of OL.
基金supported by the National Natural Science Foundation of China under grant No. 50605043
文摘Powdering/exfoliating of coatings and scratching galvanized steels and high strength steels （HSS）, are the main forms of surface damage in the forming of which result in increased die maintenance cost and scrap rate. In this study, a special rectangular box was developed to investigate the behavior and characteristics of surface damage in sheet metal forming （SMF） processes. U-channel forming tests were conducted to study the effect of tool hardness on surface damage in the forming of high strength steels and galvanized steels （hot-dip galvanized and galvannealed steels）. Experimental results indicate that sheet deformation mode influences the severity of surface damage in SMF and surface damage occurs easily at the regions where sheet specimen deforms under the action of compressive stress. Die corner is the position where surface damage initiates. For HSS sheet, surface damage is of major interest due to high forming pressure. The HSS and hot-dip galvanized steels show improved ability of damage-resistance with increased hardness of the forming tool. However, for galvannealed steel it is not the forming tool with the highest hardness value that performs best.
基金Supported by National Natural Science Foundation of China(Grant No.51375201)CSAE(Beijing)Automotive Lightweight Technology Research Institute Development Fund Project of China
文摘The use of ultra-high strength steels through sheet metal forming process offers a practical solution to the lightweight design of vehicles.However,sheet metal forming process not only produces desirable changes in material properties but also causes material damage that may adversely influence the service performance of the material formed.Thus,an investigation is conducted to experimentally quantify such influence for a commonly used steel（the 22MnB5 steel） based on the hot and cold forming processes.For each process,a number of samples are used to conduct a uniaxial tensile test to simulate the forming process.After that,some of the samples are trimmed into a standard shape and then uniaxially extended until fracture to simulate the service stage.Finally,a microstructure test is conducted to analyze the microdefects of the remaining samples.Based on the results of the first two tests,the effect of material damage on the service performance of 22MnB5 steel is analyzed.It is found that the material damages of both the hot and cold forming processes cause reductions in the service performance,such as the failure strain,the ultimate stress,the capacity of energy absorption and the ratio of residual strain.The reductions are generally lower and non-linear in the former process but higher and linear in the latter process.Additionally,it is found from the microstructure analysis that the difference in the reductions of the service performance of 22MnB5 by the two forming processes is driven by the difference in the micro damage mechanisms of the two processes.The findings of this research provide a useful reference in terms of the selection of sheet metal forming processes and the determination of forming parameters for 22MnB5.
基金Project supported by the Research Committee of The Hong Kong Polytechnic University (No.G-YX34).
文摘This paper presents the application of anisotropic damage theory to the study of forming limit diagram of A12024T3 aluminum alloy sheet. In the prediction of limiting strains of the aluminum sheet structure, a finite element cell model has been constructed. The cell model consists of two phases, the aluminum alloy matrix and the intermetallic cluster. The material behavior of the aluminum alloy matrix is described with a fully coupled elasto-plastic damage constitutive equation. The intermetallic cluster is assumed to be elastic and brittle. By varying the stretching ratio, the limiting strains of the sheet under biaxial stretching have been predicted by using the necking criterion proposed. The prediction is in good agreement with the experimental findings. Moreover, the finite element cell model can provide information for understanding the microscopic damage mechanism of the aluminum alloy. Over-estimation of the limit strains may result if the effect of material damage is ignored in the sheet metal forming study.
文摘Ductile damage often occurs during metal forming processes due to the large thermo-elasto （visco） plastic flow Iocalisation. This paper presents a numerical methodology, which aims to virtually improve any metal forming processes. The methodology is based on elastoplastic constitutive equations accounting for nonlinear mixed isotropic and kinematic hardening strongly coupled with isotropic ductile damage. An adaptive remeshing scheme based on geometrical and physical error estimates including a kill element procedure is used. Some numerical results are presented to show the capability of the model to predict the damage initiation and growth during the metal forming processes.
基金Supported by the National Natural Science Foundation of China(5137520151775227)。
文摘The effects of forming damage are analyzed,which occur during hot stamping process,on the load-carrying capacity and failure mode of hot stamped beams.A damage-coupled pre-forming constitutive model was proposed,in which the damage during hot stamping process was introduced into the service response.The constitutive model was applied into the three-point bending simulation of a hot stamped beam,and then the influences of forming damage on the load-carrying capacity and cracks propagation were investigated.The results show that the forming damage reduces the maximum load capacity of the hot stamped beam by 7.5%.It also causes the crack to occur earlier and promotes crack to propagate along the radial direction of the punch.
基金Project(50574061) supported by the National Natural Science Foundation of China
文摘Low-permeability dense reservoirs,including micro-fractured reservoirs,are commonly characterized by high content of clay substances,high original water saturation,high sensitivity to invasive fluids,high capillary pressure,complicated structure and anisotropic,high flow-resistance and micro pore throats etc,.Generally they also have lots of natural micro fractures,probably leading to stress sensibility.Main damaging factors in such reservoirs are water-sensibility and water-blocking caused by invasive fluids during drilling and production operations.Once damaged,formation permeability can rarely recovered.Numerous studies have shown that damaging extent of water-blocking ranges from 70% to 90%.Main damaging mechanisms and influencing factors of water-blocking were systematically analyzed.Also some feasible precaution or treating approaches of water-blocking were put forward.In a laboratory setting,a new multi-functional drilling fluid composed mainly of amphion polymer,sulfonation polymer,high effectively preventive water-blocking surfactants,ideal packing temporary bridging agents(TBA) and film-forming agents,etc.,were developed.New low-damage drilling fluids has many advantages,such as good rheological properties,excellent effectiveness of water-blocking prevention,good temporary plugging effect,low filtration and ultra-low permeability(API filtration≤5 mL,HTHP filtration≤10 mL,mud cake frictional coefficient≤0.14,permeability recovery>81%),can efficiently prevent or minimize damage,preserve natural formation and enhance comprehensive development-investment effect in TUHA Jurassic dense sandstone reservoir formation with low-permeability,the only one developing integrated condense gas field.Some references can be provided to similar reservoir formations.
基金supported by the National Key Research and Development Program of China(No.2018YFA0707300)the National Natural Science Foundation of China(Nos.51901151,51905372,52275362,52171122)China Postdoctoral Science Foundation(Nos.2020M680918,2021T140503)。
文摘The current study performed a finite element analysis of the strain localization behavior of a voided ductile material using a non-local plasticity formulation in which the yield strength depends on both an equivalent plastic strain measurement (hardening parameter) and Laplacian equivalent. The introduction of gradient terms to the yield function was found to play an important role in simulating the strain localization behavior of the voided ductile material. The effect of the mesh size and characteristic length on the strain localization were also investigated. An FEM simulation based on the proposed non-local plasticity revealed that the load-strain curves of the voided ductile material subjected to plane strain tension converged to one curve, regardless of the mesh size. In addition, the results using non-local plasticity also exhibited that the dependence of the deformation behavior of the material on the mesh size was much less sensitive than that with classical local plasticity and could be succe
