Development and Application of the Professional Software for Formulation Optimization Design

Aim To research the computer optimization design in material formulation. Methods The professional software including experimental design, mathematical modeling and optimization of single and multiple purposes was developed and applied to the formulation design for the flame retardant ethylenepropylene-diene mischpolymer (EPDM) insulation composites. With the aid of equipartition design, the mathematical models were established based on the optimization of the four properties (limiting oxygen index, volume resistance, tensile strength and elongation). Results The models from limited experiments had good significance, and the modeling analysis conclusions were in good agreement with later experiments. The optimization formulation was practically excellent. Conclusion The software enables practically a comprehensive multi-purpose use of material formulation research.

Optimization of Proportion and Formulation Process of Jinweng Granule by D-Optimal Mixture Design

[Objectives] To study the optimal proportion and formulation process of Jinweng granule,the physicochemical properties of the optimal preparing process was observed. [Methods] Adopting the D-optimal mixture design method,selecting the mixing ratio of starch,dextrin,fumei powder and lactose as tested factors,and selecting the most significant factor between hygroscopicity,formability,solubility as the evaluation index,the optimal proportion of filler was examined by system experiments. Granularity,solubility,the angle of repose,and critical relative humidity( CRH) were used to evaluate the optimal proportion and formulation process of Jinweng granule. [Results]The optimal prescription of Jinweng granule is extract∶ starch∶ dextrin∶ lactose∶ fumei powder( 1∶ 0. 5∶ 0. 05∶ 0. 3∶ 0. 15),and the binder was consisted of 1% sodium carboxymethylcellulose( CMC) slurry and 3% starch syrup. The CRH of the optimum formulation process of granule is 72%,and the fluidity,solubility and granularity were qualified. [Conclusions] The process model established by D-optimum mixture design has good predictability,and the granule prepared by the optimal proportion has good repeatability,and the granule proportion and formulation process is stable and reliable.

Optimization and Testing for PCR System of Rice by Orthogonal Design

[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT...

Optimization of SRAP-PCR System of Triticum aestivum——Based on Orthogonal Design

[Objective] The aim was to optimize the technical procedure of SRAP-PCR in Triticum aestivum L. [Method] The orthogonal design L16（45） was used to optimize SRAP-PCR amplification system of wheat Fengyou 68 on five factors （Taq polymerase,Mg2＋,DNA template,dNTPs and primer） at four levels. [Result] Effects of the five factors on SRAP-PCR reaction system were Mg2＋Taq polymerasedNTPsDNA templateprimer. Finally,an optimal SRAP-PCR system was established,that was the total 20 μl reaction system containing 2.0 μl 10×PCR Buffer,2.0 U Taq polymerase,2.0 mmol/L Mg2＋,0.2 mmol/L dNTPs,40 ng DNA template and 0.6 μmol/L primer. [Conclusion] The optimum SRAP-PCR system had provided some technical foundations to conduct SRAP genetic analysis for wheat varieties.

Optimization of RAPD-PCR Reaction System for Glycyrrhiza uralensis Based on Orthogonal Design

[Objective] The aim was to obtain the optimum RAPD-PCR reaction system for Glycyrrhiza uralensis.[Method] Orthogonal design was adopted to screen the suitable concentration of four major factors（dNTPs,primers,Taq polymerase and DNA template） in PCR reaction system.[Result] The optimal reaction system obtained by orthogonal design was 25 μl in total volume,containing 2.5 μl of 10×PCR buffer solution（include MgCl2）,2.5 μl of 10 mmol/L dNTPs,2 μl（100 ng） of DNA template,2 μl of 10 μmol/L primers,0.4 μl（5 U） of Taq polymerase;the optimum annealing temperature was 34 ℃.[Conclusion] Orthogonal design was an effective method for the optimization of RAPD-PCR reaction system for G.uralensis.

Cavitation Optimization for a Centrifugal Pump Impeller by Using Orthogonal Design of Experiment

Cavitation is one of the most important performance of centrifugal pumps. However, the current optimization works of centrifugal pump are mostly focusing on hydraulic efficiency only, which may result in poor cavitation performance. Therefore, it is necessary to find an appropriate solution to improve cavitation performance with acceptable efficiency. In this paper, to improve the cavitation performance of a centrifugal pump with a vaned diffuser, the influence of impeller geometric parameters on the cavitation of the pump is investigated using the orthogonal design of experiment （DOE） based on computational fluid dynamics. The impeller inlet diameter D1, inlet incidence angle Aft, and blade wrap angle ~0 are selected as the main impeller geometric parameters and the orthogonal experiment of L9（3＂3） is performed. Three-dimensional steady simulations for cavitation are conducted by using constant gas mass fraction model with second-order upwind, and the predicated cavitation performance is validated by laboratory experiment. The optimization results are obtained by the range analysis method to improve cavitation performance without obvious decreasing the efficiency of the centrifugal pump. The internal flow of the pump is analyzed in order to identify the flow behavior that can affect cavitation performance. The results show that D1 has the greatest influence on the pump cavitation and the final optimized impeller provides better flow distribution at blade leading edge. The final optimized impeller accomplishes better cavitation and hydraulic performance and the NPSHR decreases by 0.63m compared with the original one. The presented work supplies a feasible route in engineering practice to optimize a centrifugal pump impeller for better cavitation performance.

Optimization for Microbial Degumming of Ramie with Bacillus subtilis DZ_(5) in Submerged Fermentation by Orthogonal Array Design and Response Surface Methodology

As a kind of natural fiber,ramie fiber has distinctive advantages in textile application,but the application is limited due to the traditional degumming mode.Compared with the traditional degumming process,the microbial degumming process has many advantages.To obtain the optimal conditions for degumming ramie with Bacillus subtilis DZ_(5)(BS DZ_(5)),a combined statistical approach of orthogonal array design(OAD)and response surface methodology(RSM)was used.The influences of initial pH of the bacteria medium,culture temperature,shaking speed,degumming time and inoculum size on submerged fermentation degumming were evaluated by using fractional factorial design.The main factors in the analysis were culture temperature,shaking speed and initial pH.The residual gum mass fraction was used as the optimization index,and the optimal conditions for degumming were determined by central composite design and RSM.Thus with only a limited number of experiments,an optimal ramie microbial degumming condition was found as the culture temperature of 40℃,the initial pH in the culture medium of 8.5,the shaking speed of 205 r/min,the degumming time of 96 h and the inoculum size of 5%.After microbial degumming of ramie under the optimal conditions,there was only 10.6%residual gum by mass in the fiber.In addition,the effective degumming of BS DZ_(5)was also confirmed by a scanning electron microscope(SEM).

Optimization of Protective Agent Formulation for Soybean Rhizobium Strain HW-05 by Response Surface Methodology

[Objective] This study aimed to investigate the protective effects of proteins, salts, sugars and trace elements on soybean rhizobium strain HW-05 by response surface methodology. [Method] Different types, combinations, ratios and concentrations of protective agents were designed. Under simulated conditions, the optimal protective agent formulation was screened for improving the survival rate and survival time of soybean rhizobium strain HW-05. The optimal combination of significant factors was determined by Box-Behnken central composite design. [Result] Three significant factors affecting effective number of viable cells were screened, including peptone, xanthan gum and NaCl. The final concentration of each compound was optimized 0. 13% peptone, 0.011% xanthan gum, 0.30% NaCl. [Conclusion] After addition of protective agent and preservation at room temperature for six months, effective number of viable cells of soybean rhizobium strain HW-05 reached 3.185 ×10^8 CFU/ml. The survival rate of HW-05 cells was improved by more than 25% compared with the control group （2.458×10^8 CFU /ml）.

Multi-species particle swarms optimization based on orthogonal learning and its application for optimal design of a butterfly-shaped patch antenna

A new multi-species particle swarm optimization with a two-level hierarchical topology and the orthogonal learning strategy(OMSPSO) is proposed, which enhances the global search ability of particles and increases their convergence rates. The numerical results on 10 benchmark functions demonstrated the effectiveness of our proposed algorithm. Then, the proposed algorithm is presented to design a butterfly-shaped microstrip patch antenna. Combined with the HFSS solver, a butterfly-shaped patch antenna with a bandwidth of about 40.1% is designed by using the proposed OMSPSO. The return loss of the butterfly-shaped antenna is greater than 10 d B between 4.15 and 6.36 GHz. The antenna can serve simultaneously for the high-speed wireless computer networks(5.15–5.35 GHz) and the RFID systems(5.8 GHz).

A Spider Monkey Optimization Algorithm Combining Opposition-Based Learning and Orthogonal Experimental Design

As a new bionic algorithm,Spider Monkey Optimization(SMO)has been widely used in various complex optimization problems in recent years.However,the new space exploration power of SMO is limited and the diversity of the population in SMO is not abundant.Thus,this paper focuses on how to reconstruct SMO to improve its performance,and a novel spider monkey optimization algorithm with opposition-based learning and orthogonal experimental design(SMO^(3))is developed.A position updatingmethod based on the historical optimal domain and particle swarmfor Local Leader Phase(LLP)andGlobal Leader Phase(GLP)is presented to improve the diversity of the population of SMO.Moreover,an opposition-based learning strategy based on self-extremum is proposed to avoid suffering from premature convergence and getting stuck at locally optimal values.Also,a local worst individual elimination method based on orthogonal experimental design is used for helping the SMO algorithm eliminate the poor individuals in time.Furthermore,an extended SMO^(3)named CSMO^(3)is investigated to deal with constrained optimization problems.The proposed algorithm is applied to both unconstrained and constrained functions which include the CEC2006 benchmark set and three engineering problems.Experimental results show that the performance of the proposed algorithm is better than three well-known SMO algorithms and other evolutionary algorithms in unconstrained and constrained problems.

Optimization of the Formulation Process of Glucosamine Chondroitin Sulfate Tablets

[Objectives]This study was conducted to optimize the Formulation Process of glucosamine chondroitin sulfate tablets. [Methods] The orthogonal design with three levels was carried out with microcrystalline cellulose, calcium hydrophosphate and cross-linked polyvinylpyrrolidone as three factors to optimize the preparation process. [Results] When microcrystalline cellulose 200 mg/tablet, calcium hydrophosphate 150 mg/tablet, and cross-linked polyvinylpyrrolidone 80 mg/tablet were added, the angle of repose could meet the requirements of tablet pressing, and the dissolution could reach more than 95% in 30 min. The results of the orthogonal test showed that the dissolution effect of self-made tablets was faster than that of commercial products. [Conclusions] The glucosamine hydrochloride chondroitin sulfate tablets prepared by this prescription have better quality.

Optimization analysis using orthogonal array designs for magnet girder assembly of SSRF

An optimization analysis for finite element (FE) results by variance analysis method (VAM) of orthogonal array designs (OADs) was performed to improve the dynamic performance of the prototype magnet girder assembly (MGA) in the storage ring of the Shanghai Synchrotron Radiation Facility (SSRF).Seven factors were considered.The analyses show that the most important factor for the first eigenfrequency is stiffness of the support systems,and the main factor resulting in the relatively low first eigenfrequency of the MGA is its weak support systems.From the OADs optimization analysis,mechanical design of the MGA was improved,and its dynamic performance was improved obviously.

Optimization of a Centrifugal Pump Used as a Turbine Impeller By Means of an Orthogonal Test Approach

A prototype centrifugal pump with a specific speed of 110 is used to investigate and optimize the performances of a turbine for power generation.Particular attention is given to the design of the internal impeller.The internal flow field is simulated in the framework of a commercial computational fluid dynamics software(ANSYS).Four geometrical parameters of the impeller are considered,i.e.,the inlet diameter,the inlet width,the blade number,and the blade angle.The optimization is carried out on the basis of a three-level approach relying on an orthogonal test method.The results of the numerical simulations show good agreement with the experimental tests under different flow conditions.In accordance with the L9(34)design table,the head and efficiency under the rated flow rate of the nine designed schemes are calculated and processed with the method of range analysis to obtain an optimized model.

Optimal Design of High-Speed Partial Flow Pumps using Orthogonal Tests and Numerical Simulations

To investigate the influence of structural parameters on the performances and internal flow characteristics of partial flow pumps at a low specific speed of 10000 rpm,special attention was paid to the first and second stage impeller guide vanes.Moreover,the impeller blade outlet width,impeller inlet diameter,blade inclination angle,and number of blades were considered for orthogonal tests.Accordingly,nine groups of design solutions were formed,and then used as a basis for the execution of numerical simulations(CFD)aimed at obtaining the efficiency values and heads for each design solution group.The influence of impeller geometric parameters on the efficiency and head was explored,and the“weight”of each factor was obtained via a range analysis.Optimal structural parameters were finally chosen on the basis of the numerical simulation results,and the performances of the optimized model were verified accordingly(yet by means of CFD).Evidence is provided that the increase in the efficiency and head of the optimized model was 12.11%and 23.5 m,respectively,compared with those of the original model.

Optimization of PCR Reaction System for Random Single-strand DNA Pool in SELEX Technology

[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.

Joint Optimization of Channel Allocation,Link Assignment and Power Control for Device-to-Device Communication Underlaying Cellular Network

Device-to-Device(D2D) communication has been proposed to facilitate cellular network with system capacity(SC) and quality of service(QoS).We consider the design of link assignment(LA),channel allocation(CA)and power control(PC) in D2D-aided content delivery scenario for both user fairness(UF)and system throughput(ST) under QoS requirement.Due to the complexity of the problem,we decompose it into two components:CA is formulated from graph perspective to mitigate severe co-channel interference,which turns out to be the Max K-cut problem;LA and PC are jointly optimized to utilize the gain achieved from CA for supreme performance,and specifically,genetic algorithm(GA) is adopted to optimize LA,but when deriving the fitness of each chromosome,PC optimization will be involved.Thanks to numerical results,we elucidate the efficacy of our scheme.

Development of Food Formulations for Individual Nutrition

This paper proposes a method for the developing of nutrition formulations for various population groups categorized by gender, age, body type and nature of labor activity. Meat formulations were developed, taking into account dietitian re- commendations, rationality of the amino acid composition, processing characteristics, organoleptic evaluation and cost indicators.

Statistical approach for the culture conditions optimization of magnetotactic bacteria for magnetic cells production

The culture of Magnetospirillum magneticum WM-1 depends on several control factors that have great effect on the magnetic cells concentration. Investigation into the optimal culture conditions needs a large number of experiments So it is desirable to minimize the number of experiments and maximize the information gained from them. The orthogonal design of experiments and mathematical statistical method are considered as effective methods to optimize the culture condition of magnetotactic bacteria WMol for high magnetic cells concentration. The effects of the four factors, such as pH value of medium, oxygen concentration of gas phase in the serum bottle, C：C （mtartaric acid： m=succinic acid） ratio and NaNO3 concentration, are simultaneously investigated by only sixteen experiments through the orthogonal design L16（44） method. The optimal culture condition is obtained. At the optimal culture condition （ pH 7.0, an oxygen concentration 4.0%, C：C （mtartaric acid： m=succinic acid） ratio 1：2 and NaNO3 100 mg 1^-1）, the magnetic cells concentration is promoted tO 6.5×10^7 cells ml^-1, approximately 8.3% higher than that under the initial conditions. The pH value of medium is a very important factor for magnetic cells concentration. It can be Proved that the orthogonal design of experiment is of 90% confidence. Ferric iron uptake follows MichaelisoMenten kinetics with a Km of 2.5 pM and a Vmax of 0.83 min^-1.

OPTIMIZING FORMULATION OF HYBRID MODEL FOR THIN AND MODERATELY THICK PLATES

A 20 - DOF hybrid stress element based upon Mindlin plate theory is developed using the optimization design method for thin and moderately thick plates. Numerical tests consist of the convergency and performance to the plates with arbitrary thickness and shape and of the ultimate thin plate problems.

Numerical simulation and optimization of the cylinder head water jacket based on the two-phase flow boiling heat transfer

Boiling heat transfer and the controllability of the thermal load of the cylinder head were studied.The thermodynamic phase change characteristics of the cylinder head coolant were considered and the mass,momentumand energy transfers between two phases were calculated with the interface transfer submodels by using the computational fluid dynamics software CFX. Results showed that compared with the single-phase flow without considering the boiling heat transfer,the sub-cooled boiling heat transfer of the cylinder head was greatly increased. According to the results of the numerical simulation,an optimized structure of the water jacket was proposed. Finally,temperature and velocity of coolant,diameter of flow passage and mean bubble diameter that influences sub-cooled boiling were studied using the orthogonal experiment method.