BACKGROUND Increasing data indicated that long noncoding RNAs(lncRNAs)were directly or indirectly involved in the occurrence and development of tumors,including hepatocellular carcinoma(HCC).Recent studies had found t...BACKGROUND Increasing data indicated that long noncoding RNAs(lncRNAs)were directly or indirectly involved in the occurrence and development of tumors,including hepatocellular carcinoma(HCC).Recent studies had found that the expression of lncRNA HAND2-AS1 was downregulated in HCC tissues,but its role in HCC progression is unclear.Ultrasound targeted microbubble destruction mediated gene transfection is a new method to overexpress genes.AIM To study the role of ultrasound microbubbles(UTMBs)mediated HAND2-AS1 in the progression of HCC,in order to provide a new reference for the treatment of HCC.METHODS In vitro,we transfected HAND2-AS1 siRNA into HepG2 cells by UTMBs,and detected cell proliferation,apoptosis,invasion and epithelial-mesenchymal transition(EMT)by cell counting kit-8 assay,flow cytometry,Transwell invasion assay and Western blotting,respectively.In addition,we transfected miR-837-5p mimic into UTMBs treated cells and observed the changes of cell behavior.Next,the UTMBs treated HepG2 cells were transfected together with miR-837-5p mimic and tissue inhibitor of matrix metalloproteinase-2(TIMP2)overexpression vector,and we detected cell proliferation,apoptosis,invasion and EMT.In vivo,we established a mouse model of subcutaneous transplantation of HepG2 cells and observed the effect of HAND2-AS1 silencing on tumor formation ability.RESULTS We found that UTMBs carrying HAND2-AS1 restricted cell proliferation,invasion,and EMT,encouraged apoptosis,and HAND2-AS1 silencing eliminated the effect of UTMBs.Additionally,miR-873-5p targets the gene HAND2-AS1,which also targets the 3’UTR of TIMP2.And miR-873-5p mimic counteracted the impact of HAND2-AS1.Further,miR-873-5p mimic solely or in combination with pcDNA-TIMP2 had been transformed into HepG2 cells exposed to UTMBs.We discovered that TIMP2 reversed the effect of miR-873-5p mimic caused by the blocked signalling cascade for matrix metalloproteinase(MMP)2/MMP9.In vivo results showed that HAND2-AS1 silencing significantly inhibited tumor formation in mice.CONCLUSION LncRNA HAND2-AS1 promotes TIMP2 expression by targeting miR-873-5p to inhibit HepG2 cell growth and delay HCC progression.展开更多
A standard hepaticojejunostomy technique might be difficult to perform, especially when the bile duct is small and located deep in the liver hilum. Herein we present a new procedure, the Hand-Fan technique, that was u...A standard hepaticojejunostomy technique might be difficult to perform, especially when the bile duct is small and located deep in the liver hilum. Herein we present a new procedure, the Hand-Fan technique, that was used to enhance the exposure and ease the performance of these challenging anastomoses. Thirty-one patients who had had hepaticojejunostomy with this technique for bile duct injury and other benign biliary pathologies from July 2004 to June 2011 were included into the study. Median postoperative hospital stay was 7 days (6-25 days) and median follow-up time was 33 months (2-84 months). Liver function tests revealed that the blood bilirubin levels of the patients were normalized after hepaticojejunostomy. Follow-up showed that there were no signs of clinical recurrence or impaired bile flow. The Hand-Fan technique considerably facilitates challenging hepaticojejunostomies. Surgeon’s comfort is exceptional and the clinical results are satisfactory.展开更多
It has been proved by the ancient and modemacupuncture practices that needling techniques are ofvital importance in the acupuncture treatment ofdiseases.At present,much has been reported aboutthe researches on acupunc...It has been proved by the ancient and modemacupuncture practices that needling techniques are ofvital importance in the acupuncture treatment ofdiseases.At present,much has been reported aboutthe researches on acupuncture techniques,which canbe roughly divided into the following three types:reinforcing,reducing,and even methods.Throughlong years of study and clinical practice,Dr.展开更多
[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistr...[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.展开更多
With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and...With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and new home price index for the empirical analysis, thedata related to the cointegration analysis found that the result of the first -tier cities real estatemarket in China, the new home price index is the significant factors influencing the second -hand house price indexi For Beijing, Shanghai second - hand housing and new home price in-dex time series johans test, found that there exists cointegration relationship between two varia-bles,the new city real estate market prices out of a line on the secondary market have clearguide. Therefore, the real estate market regulation aiming at the first -tier cities and the＂housing stock＂ should take the second - hand housing market as the main direction, startingwith the sale price and influencing factors of new houses. At the same time, in different cities,we should adhere to the city＇ s policies, reflect the policy differentiation, promote the reformof the real estate supply side, and promote the return of housing properties.展开更多
This paper presents a web-based integrated system for on-line sensory fabric hand evaluation. The methods of fuzzy techniques, neural networks, classical factorial analysis and other data analysis are used in the syst...This paper presents a web-based integrated system for on-line sensory fabric hand evaluation. The methods of fuzzy techniques, neural networks, classical factorial analysis and other data analysis are used in the system to analyze the objective and subjective data, and to build the relationship between them. Given the objective data of a new fabric sample, the system can provide its sensory hand data and its total hand grade. In meantime, the total hand grade can be obtained directly from the sensory fabric hand data if provided. The sensory evaluation system is developed in Internet environment using Java language and SQL server database management system.展开更多
Introduction: Congenital talipes equinovarus (CTEV) is the commonest musculoskeletal deformity worldwide. The Ponseti technique is the goal standard for clubfoot treatment. During the correction phase, the provider’s...Introduction: Congenital talipes equinovarus (CTEV) is the commonest musculoskeletal deformity worldwide. The Ponseti technique is the goal standard for clubfoot treatment. During the correction phase, the provider’s right hand manipulates the right foot and the left hand, left foot. Often, one foot is ready for Achilles tenotomy before the other in bilateral clubfoot. Objective: To determine the effect of the provider’s hand dominance would have on bilateral clubfoot treated with the Ponseti technique. Method: This was a prospective cross-sectional study that analyzed idiopathic bilateral clubfoot patients aged 0 - 5 years and treated using the Ponseti technique at FMC Umuahia from October 2019 to September 2020. Informed consent and ethical clearance were obtained. The Pirani scores were obtained and compared at presentation and at each clinic visit. All trained manipulators were right-handed. Two-tailed t-test was used and a p-value less than 0.05 was deemed significant. Results: Forty-seven patients participated in the study with an M:F of 2.6:1 and mean age of 13.79 ± 13.39 months. Thirty-six patients (76.6%) had the same Pirani score on both feet at presentation, while the right and left feet were more severely affected in 8 and 3 cases respectively. The mean number of casts before readiness for tenotomy was 4.95 on the right and 5.28 on the left with p-value of 0.042. Conclusion: Though the right foot had a worse mean Pirani score on presentation, however, it required fewer casts before readiness for tenotomy than the left.展开更多
Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contaminatio...Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contamination of second-order spectra(SOS)which will introduce some undesirable uncertainties at the red side of the spectra.In this paper,we test the effect of SOS and propose a method to correct it in the time domain spectroscopic data using the simultaneously observed comparison stars.Based on the reverberation mapping(RM)data of NGC 5548 in2019,one of the most intensively monitored AGNs by the Lijiang 2.4 m telescope,we find that the scientific object,comparison star,and spectrophotometric standard star can jointly introduce up to~30%SOS for Grism 14.This irregular but smooth SOS significantly affects the flux density and profile of the emission line,while having little effect on the light curve.After applying our method to each spectrum,we find that the SOS can be corrected effectively.The deviation between corrected and intrinsic spectra is~2%,and the impact of SOS on time lag is very minor.This method makes it possible to obtain the HαRM measurements from archival data provided that the spectral shape of the AGN under investigation does not have a large change.展开更多
The radio telescope possesses high sensitivity and strong signal collection capabilities.While receiving celestial radiation signals,it also captures Radio Frequency Interferences(RFIs)introduced by human activities.R...The radio telescope possesses high sensitivity and strong signal collection capabilities.While receiving celestial radiation signals,it also captures Radio Frequency Interferences(RFIs)introduced by human activities.RFI,as signals originating from sources other than the astronomical targets,significantly impacts the quality of astronomical data.This paper presents an RFI fast mitigation algorithm based on block Least Mean Square(LMS)algorithm.It enhances the traditional adaptive LMS filter by grouping L adjacent time-sampled points into one block and applying the same filter coefficients for filtering within each block.This transformation reduces multiplication calculations and enhances algorithm efficiency by leveraging the time-domain convolution theorem.The algorithm is tested using baseband data from the Parkes 64 m radio telescope's pulsar observations and simulated data.The results confirm the algorithm's effectiveness,as the pulsar profile after RFI mitigation closely matches the original pulsar profile.展开更多
文摘BACKGROUND Increasing data indicated that long noncoding RNAs(lncRNAs)were directly or indirectly involved in the occurrence and development of tumors,including hepatocellular carcinoma(HCC).Recent studies had found that the expression of lncRNA HAND2-AS1 was downregulated in HCC tissues,but its role in HCC progression is unclear.Ultrasound targeted microbubble destruction mediated gene transfection is a new method to overexpress genes.AIM To study the role of ultrasound microbubbles(UTMBs)mediated HAND2-AS1 in the progression of HCC,in order to provide a new reference for the treatment of HCC.METHODS In vitro,we transfected HAND2-AS1 siRNA into HepG2 cells by UTMBs,and detected cell proliferation,apoptosis,invasion and epithelial-mesenchymal transition(EMT)by cell counting kit-8 assay,flow cytometry,Transwell invasion assay and Western blotting,respectively.In addition,we transfected miR-837-5p mimic into UTMBs treated cells and observed the changes of cell behavior.Next,the UTMBs treated HepG2 cells were transfected together with miR-837-5p mimic and tissue inhibitor of matrix metalloproteinase-2(TIMP2)overexpression vector,and we detected cell proliferation,apoptosis,invasion and EMT.In vivo,we established a mouse model of subcutaneous transplantation of HepG2 cells and observed the effect of HAND2-AS1 silencing on tumor formation ability.RESULTS We found that UTMBs carrying HAND2-AS1 restricted cell proliferation,invasion,and EMT,encouraged apoptosis,and HAND2-AS1 silencing eliminated the effect of UTMBs.Additionally,miR-873-5p targets the gene HAND2-AS1,which also targets the 3’UTR of TIMP2.And miR-873-5p mimic counteracted the impact of HAND2-AS1.Further,miR-873-5p mimic solely or in combination with pcDNA-TIMP2 had been transformed into HepG2 cells exposed to UTMBs.We discovered that TIMP2 reversed the effect of miR-873-5p mimic caused by the blocked signalling cascade for matrix metalloproteinase(MMP)2/MMP9.In vivo results showed that HAND2-AS1 silencing significantly inhibited tumor formation in mice.CONCLUSION LncRNA HAND2-AS1 promotes TIMP2 expression by targeting miR-873-5p to inhibit HepG2 cell growth and delay HCC progression.
文摘A standard hepaticojejunostomy technique might be difficult to perform, especially when the bile duct is small and located deep in the liver hilum. Herein we present a new procedure, the Hand-Fan technique, that was used to enhance the exposure and ease the performance of these challenging anastomoses. Thirty-one patients who had had hepaticojejunostomy with this technique for bile duct injury and other benign biliary pathologies from July 2004 to June 2011 were included into the study. Median postoperative hospital stay was 7 days (6-25 days) and median follow-up time was 33 months (2-84 months). Liver function tests revealed that the blood bilirubin levels of the patients were normalized after hepaticojejunostomy. Follow-up showed that there were no signs of clinical recurrence or impaired bile flow. The Hand-Fan technique considerably facilitates challenging hepaticojejunostomies. Surgeon’s comfort is exceptional and the clinical results are satisfactory.
文摘It has been proved by the ancient and modemacupuncture practices that needling techniques are ofvital importance in the acupuncture treatment ofdiseases.At present,much has been reported aboutthe researches on acupuncture techniques,which canbe roughly divided into the following three types:reinforcing,reducing,and even methods.Throughlong years of study and clinical practice,Dr.
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ0932)National Key Technology R & D Program of China(20068AI06A15-11)~~
文摘[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.
文摘With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and new home price index for the empirical analysis, thedata related to the cointegration analysis found that the result of the first -tier cities real estatemarket in China, the new home price index is the significant factors influencing the second -hand house price indexi For Beijing, Shanghai second - hand housing and new home price in-dex time series johans test, found that there exists cointegration relationship between two varia-bles,the new city real estate market prices out of a line on the secondary market have clearguide. Therefore, the real estate market regulation aiming at the first -tier cities and the＂housing stock＂ should take the second - hand housing market as the main direction, startingwith the sale price and influencing factors of new houses. At the same time, in different cities,we should adhere to the city＇ s policies, reflect the policy differentiation, promote the reformof the real estate supply side, and promote the return of housing properties.
基金supported by the joint Sino-French Advanced Research Program(No:PRA-SI-01-05)the National Natural Science Foundation(60004006)from P.R.China.
文摘This paper presents a web-based integrated system for on-line sensory fabric hand evaluation. The methods of fuzzy techniques, neural networks, classical factorial analysis and other data analysis are used in the system to analyze the objective and subjective data, and to build the relationship between them. Given the objective data of a new fabric sample, the system can provide its sensory hand data and its total hand grade. In meantime, the total hand grade can be obtained directly from the sensory fabric hand data if provided. The sensory evaluation system is developed in Internet environment using Java language and SQL server database management system.
文摘Introduction: Congenital talipes equinovarus (CTEV) is the commonest musculoskeletal deformity worldwide. The Ponseti technique is the goal standard for clubfoot treatment. During the correction phase, the provider’s right hand manipulates the right foot and the left hand, left foot. Often, one foot is ready for Achilles tenotomy before the other in bilateral clubfoot. Objective: To determine the effect of the provider’s hand dominance would have on bilateral clubfoot treated with the Ponseti technique. Method: This was a prospective cross-sectional study that analyzed idiopathic bilateral clubfoot patients aged 0 - 5 years and treated using the Ponseti technique at FMC Umuahia from October 2019 to September 2020. Informed consent and ethical clearance were obtained. The Pirani scores were obtained and compared at presentation and at each clinic visit. All trained manipulators were right-handed. Two-tailed t-test was used and a p-value less than 0.05 was deemed significant. Results: Forty-seven patients participated in the study with an M:F of 2.6:1 and mean age of 13.79 ± 13.39 months. Thirty-six patients (76.6%) had the same Pirani score on both feet at presentation, while the right and left feet were more severely affected in 8 and 3 cases respectively. The mean number of casts before readiness for tenotomy was 4.95 on the right and 5.28 on the left with p-value of 0.042. Conclusion: Though the right foot had a worse mean Pirani score on presentation, however, it required fewer casts before readiness for tenotomy than the left.
基金funded by the National Key R&D Program of China with No.2021YFA1600404the National Natural Science Foundation of China(NSFC+6 种基金grant Nos.11991051,12303022,12373018,12203096,12103041,12073068)Yunnan Fundamental Research Projects(grant Nos.202301AT070339,202301AT070358)Yunnan Postdoctoral Foundation Funding Project,the Yunnan Province Foundation(202001AT070069)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2022058)the Topnotch Young Talents Program of Yunnan Province,Special Research Assistant Funding Project of Chinese Academy of Sciencesthe science research grants from the China Manned Space Project with No.CMS-CSST-2021-A06Funding for the telescope has been provided by the Chinese Academy of Sciences and the People’s Government of Yunnan Province。
文摘Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contamination of second-order spectra(SOS)which will introduce some undesirable uncertainties at the red side of the spectra.In this paper,we test the effect of SOS and propose a method to correct it in the time domain spectroscopic data using the simultaneously observed comparison stars.Based on the reverberation mapping(RM)data of NGC 5548 in2019,one of the most intensively monitored AGNs by the Lijiang 2.4 m telescope,we find that the scientific object,comparison star,and spectrophotometric standard star can jointly introduce up to~30%SOS for Grism 14.This irregular but smooth SOS significantly affects the flux density and profile of the emission line,while having little effect on the light curve.After applying our method to each spectrum,we find that the SOS can be corrected effectively.The deviation between corrected and intrinsic spectra is~2%,and the impact of SOS on time lag is very minor.This method makes it possible to obtain the HαRM measurements from archival data provided that the spectral shape of the AGN under investigation does not have a large change.
基金supported by the National Key R&D Program of China(Nos.2021YFC2203502 and 2022YFF0711502)the National Natural Science Foundation of China(NSFC)(12173077 and 12073067)+7 种基金the Tianshan Innovation Team Plan of Xinjiang Uygur Autonomous Region(2022D14020)the Tianshan Talent Project of Xinjiang Uygur Autonomous Region(2022TSYCCX0095)the Scientific Instrument Developing Project of the Chinese Academy of Sciences(grant No.PTYQ2022YZZD01)China National Astronomical Data Center(NADC)the Operation,Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments,budgeted from the Ministry of Finance of China(MOF)and administrated by the Chinese Academy of Sciences(CAS)Natural Science Foundation of Xinjiang Uygur AutonomousRegion(2022D01A360)the CAS“Light of West China”program under No.2022-XBQNXZ-012supported by Astronomical Big Data Joint Research Center,cofounded by National Astronomical Observatories,Chinese Academy of Sciences。
文摘The radio telescope possesses high sensitivity and strong signal collection capabilities.While receiving celestial radiation signals,it also captures Radio Frequency Interferences(RFIs)introduced by human activities.RFI,as signals originating from sources other than the astronomical targets,significantly impacts the quality of astronomical data.This paper presents an RFI fast mitigation algorithm based on block Least Mean Square(LMS)algorithm.It enhances the traditional adaptive LMS filter by grouping L adjacent time-sampled points into one block and applying the same filter coefficients for filtering within each block.This transformation reduces multiplication calculations and enhances algorithm efficiency by leveraging the time-domain convolution theorem.The algorithm is tested using baseband data from the Parkes 64 m radio telescope's pulsar observations and simulated data.The results confirm the algorithm's effectiveness,as the pulsar profile after RFI mitigation closely matches the original pulsar profile.
