BACKGROUND： As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 （CRP2） has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previo...BACKGROUND： As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 （CRP2） has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family H members in stem or precursor cells has not been described. OBJECTIVE： To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING： An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS： Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS： Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfactory stem cells. MAIN OUTCOME MEASURES： The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS： CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nucleus and the cytoplasm. CONCLUSION： Confirmation of the expression and distribution of CRP2 in olfactory stem cells.展开更多
4个半LIM结构域蛋白1(Four and a half LIM domains protein 1,FHL1)属于LIM-only家族成员之一,通过LIM结构域与其他蛋白相互作用,在转录调节、细胞运动和细胞增殖等功能中发挥重要作用,而且与肺癌、乳腺癌、胃肠道肿瘤、膀胱癌等多种...4个半LIM结构域蛋白1(Four and a half LIM domains protein 1,FHL1)属于LIM-only家族成员之一,通过LIM结构域与其他蛋白相互作用,在转录调节、细胞运动和细胞增殖等功能中发挥重要作用,而且与肺癌、乳腺癌、胃肠道肿瘤、膀胱癌等多种肿瘤的发生发展密切相关。已有研究证实,FHL1在肌肉发育、肌病和心血管疾病中的重要性。本文综述了FHL1在多种肿瘤发生发展中的作用,旨在为临床寻找新型的生物标记物和药物作用靶点提供思路。展开更多
FHL(four and half LIM domains)是LIM-only蛋白家族的重要成员。FHL家族包括FHL1～FHL5共5个成员,它们具有组织特异性。研究发现,FHL参与转录调节、信号转导、凋亡等,是细胞生长、分化的重要调节因子。已证实FHL1、FHL2与不同组织来源...FHL(four and half LIM domains)是LIM-only蛋白家族的重要成员。FHL家族包括FHL1～FHL5共5个成员,它们具有组织特异性。研究发现,FHL参与转录调节、信号转导、凋亡等,是细胞生长、分化的重要调节因子。已证实FHL1、FHL2与不同组织来源肿瘤的发生和发展有关。FHL表达水平是某些肿瘤预后的相关因素。研究FHL与肿瘤的关系,有利于阐明肿瘤的病理生理机制。展开更多
目的:研究结肠癌患者癌组织中人吻素1(kisspeptin 1,KiSS-1)、4个半LIM结构域蛋白1(four and a half LIM domains protein 1,FHL1)的表达与临床意义。方法:选择唐山市人民医院2010年10月至2011年5月收治的69例接受手术及术后化疗的结肠...目的:研究结肠癌患者癌组织中人吻素1(kisspeptin 1,KiSS-1)、4个半LIM结构域蛋白1(four and a half LIM domains protein 1,FHL1)的表达与临床意义。方法:选择唐山市人民医院2010年10月至2011年5月收治的69例接受手术及术后化疗的结肠癌患者作为研究对象,分别采用免疫组织化学技术和RT-PCR技术检查KiSS-1与FHL1在结肠癌组织中的表达,随后结合患者临床信息及随访资料分析KiSS-1与FHL1表达在结肠癌中的临床意义。结果:结肠癌组织中KiSS-1,FHL1蛋白及mRNA均低于癌周组织,差异具有统计学意义(P<0.05);KiSS-1,FHL1表达与肿瘤分化程度、淋巴结转移有关(P<0.05);KiSS-1和FHL1阳性表达患者的5年总体生存率明显高于阴性表达患者,差异具有统计学意义(P<0.05);结肠癌组织中KiSS-1与FHL1表达呈正相关(r=0.698,P=0.012),均是结肠癌患者预后的独立影响因素(P<0.05)。结论:KiSS-1与FHL1在结肠癌组织中表达下降或缺失,联合检测KiSS-1与FHL1蛋白对判断结肠癌生物学行为、评估患者预后具有一定的临床价值。展开更多
目的:观察FHL1(Four and a half LIM domains protein1)基因在P19细胞向心肌细胞诱导分化过程中表达水平的变化,探讨FHL1基因与心肌细胞分化之间的关系。方法:P19细胞经1%二甲基亚砜(DMSO)诱导悬浮培养4天形成细胞聚集体,将聚集体用生...目的:观察FHL1(Four and a half LIM domains protein1)基因在P19细胞向心肌细胞诱导分化过程中表达水平的变化,探讨FHL1基因与心肌细胞分化之间的关系。方法:P19细胞经1%二甲基亚砜(DMSO)诱导悬浮培养4天形成细胞聚集体,将聚集体用生长培养基黏附培养至14天,观察细胞跳动情况,用肌钙蛋白I(cTnI)抗体行Western Blot以鉴定心肌细胞分化,并采用RT-PCR、Western Blot技术在细胞分化成熟的不同时段检测P19细胞中FHL1基因mRNA和蛋白的表达水平。结果:DMSO诱导4天后,用生长培养基黏附培养至第8天,P19细胞出现自发性节律跳动的细胞团片,Western Blot检测cTnI蛋白呈阳性。FHL1基因在P19细胞向心肌细胞分化过程中,随细胞分化成熟该基因表达水平逐渐上调,Western Blot检测结果与RT-PCR检测结果基本一致。FHL1基因在分化第0～8天表达显著增高,各时间点之间差异显著(P<0.05);第8～14天表达趋于稳定,各时间点之间差异无显著性(P>0.05)。结论:FHL1基因在P19细胞向心肌细胞分化过程中表达逐渐上调,可能参与心肌细胞的分化和发育。展开更多
文摘BACKGROUND： As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 （CRP2） has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family H members in stem or precursor cells has not been described. OBJECTIVE： To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING： An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS： Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS： Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfactory stem cells. MAIN OUTCOME MEASURES： The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS： CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nucleus and the cytoplasm. CONCLUSION： Confirmation of the expression and distribution of CRP2 in olfactory stem cells.
文摘4个半LIM结构域蛋白1(Four and a half LIM domains protein 1,FHL1)属于LIM-only家族成员之一,通过LIM结构域与其他蛋白相互作用,在转录调节、细胞运动和细胞增殖等功能中发挥重要作用,而且与肺癌、乳腺癌、胃肠道肿瘤、膀胱癌等多种肿瘤的发生发展密切相关。已有研究证实,FHL1在肌肉发育、肌病和心血管疾病中的重要性。本文综述了FHL1在多种肿瘤发生发展中的作用,旨在为临床寻找新型的生物标记物和药物作用靶点提供思路。
文摘FHL(four and half LIM domains)是LIM-only蛋白家族的重要成员。FHL家族包括FHL1～FHL5共5个成员,它们具有组织特异性。研究发现,FHL参与转录调节、信号转导、凋亡等,是细胞生长、分化的重要调节因子。已证实FHL1、FHL2与不同组织来源肿瘤的发生和发展有关。FHL表达水平是某些肿瘤预后的相关因素。研究FHL与肿瘤的关系,有利于阐明肿瘤的病理生理机制。
文摘目的:研究结肠癌患者癌组织中人吻素1(kisspeptin 1,KiSS-1)、4个半LIM结构域蛋白1(four and a half LIM domains protein 1,FHL1)的表达与临床意义。方法:选择唐山市人民医院2010年10月至2011年5月收治的69例接受手术及术后化疗的结肠癌患者作为研究对象,分别采用免疫组织化学技术和RT-PCR技术检查KiSS-1与FHL1在结肠癌组织中的表达,随后结合患者临床信息及随访资料分析KiSS-1与FHL1表达在结肠癌中的临床意义。结果:结肠癌组织中KiSS-1,FHL1蛋白及mRNA均低于癌周组织,差异具有统计学意义(P<0.05);KiSS-1,FHL1表达与肿瘤分化程度、淋巴结转移有关(P<0.05);KiSS-1和FHL1阳性表达患者的5年总体生存率明显高于阴性表达患者,差异具有统计学意义(P<0.05);结肠癌组织中KiSS-1与FHL1表达呈正相关(r=0.698,P=0.012),均是结肠癌患者预后的独立影响因素(P<0.05)。结论:KiSS-1与FHL1在结肠癌组织中表达下降或缺失,联合检测KiSS-1与FHL1蛋白对判断结肠癌生物学行为、评估患者预后具有一定的临床价值。
