Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian developmen...Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian development red swamp crayfish （Procambarus clarkii）. The results showed that all the three differ- ent methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree, with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in order immersion method 〉 injection method 〉 topical apphcation method 〉 control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish, with the order of immersion method 32.5%, injection method 20%, topical application 17.5% and the control group 17.5 %. Taking the synchro- nization spawning rate, final spawning rate and mortality into account, the order was the immersion method 〉 injection method 〉 topical application method 〉 control group.展开更多
It is difficult to build an effective water flooding displacement pressure system in the middle section of a horizontal well in an ultra-low permeability sandstone reservoir.To solve this problem,this study proposes t...It is difficult to build an effective water flooding displacement pressure system in the middle section of a horizontal well in an ultra-low permeability sandstone reservoir.To solve this problem,this study proposes to use packers,sealing cannula and other tools in the same horizontal well to inject water in some fractures and produce oil from other fractures.This new energy supplement method forms a segmental synchronous injection-production system in a horizontal well.The method can reduce the distance between the injection end and the production end,and quickly establish an effective displacement system.Changing the displacement between wells to displacement between horizontal well sections,and point water flooding to linear uniform water flooding,the method can enhance water sweeping volume and shorten waterflooding response period.The research shows that:(1)In the synchronous injection and production of horizontal well in an ultra-low-permeability sandstone reservoir,the water injection section should select the section where the natural fractures and artificial fractures are in the same direction or the section with no natural fractures,and the space between two sections should be 60?80 m.(2)In addition to controlling injection pressure,periodic water injection can be taken to reduce the risk of re-opening and growth of natural fractures or formation fracture caused by the gradual increase of water injection pressure with water injection going on.(3)Field tests have verified that this method can effectively improve the output of single well and achieve good economic benefits,so it can be widely used in the development of ultra-low permeability sandstone reservoirs.展开更多
目的:通过比较绒毛膜促性腺激素释放激素激动剂(GnRH-a)降调节时间的不同对卵泡直径、子宫内膜厚度以及血卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平的影响,从而寻求体外受精-胚胎移植(IVF-ET)超促排卵相对合适的启动时间。方法...目的:通过比较绒毛膜促性腺激素释放激素激动剂(GnRH-a)降调节时间的不同对卵泡直径、子宫内膜厚度以及血卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平的影响,从而寻求体外受精-胚胎移植(IVF-ET)超促排卵相对合适的启动时间。方法:选择2009年在我中心进行IVF-ET的患者42例,全部采用黄体中期GnRH-a降调节的长方案。①根据GnRH-a降调节时间的不同分为降调节10、15、18 d 3组,分别进行B超监测卵泡的直径、子宫内膜厚度,抽血测定FSH、LH、E2水平,比较降调节时间的不同对卵泡直径、内膜厚度、性激素水平的影响。②降调节后的1、7、10、14 d,分别比较注射GnRH-a前以及注射后2、3 h血FSH、LH的变化。结果:①降调节10、15、18 d不同直径卵泡所占百分比:3～4 mm:16.8%vs 7.09%vs 10.38%;4.5～7.0 mm:80.24%vs89.55%vs 84.62;7.5～10 mm:2.96%vs 3.36%vs 5%。3～4 mm直径的卵泡在降调节10 d所占比例,与15、18 d有显著差异(P<0.05);4.5～7 mm直径的卵泡在15 d所占比例与10、18 d有显著差异(P<0.05);②降调节10、15、18 d内膜厚度(mm):7.73±2.48 vs 5.41±0.79 vs 5.24±0.85,降调节10 d内膜明显厚于15、18 d,有显著差异(P<0.05);③降调节10、15、18 d血FSH(mIU/ml):3.70±1.10 vs 3.51±0.72 vs 3.47±0.61;血LH(mIU/ml):1.23±1 vs 1.09±0.47 vs 1.22±0.72;E2:41.84±36.81 vs 32.84±14.32 vs 9.50±8.23,无显著差异。研究2:降调节后的1、7、10、14 d,注射GnRH-a后2、3 h,血FSH、LH都有升高,其中D1 d升高幅度最大,1.87±1.49 vs 13.33±7.81;1.06±1.13 vs 47.40±29.97,有统计学差异(P<0.05)。结论:在IVF-ET超促排卵长方案中,当GnRH-a降调节10 d,子宫内膜厚度、血FSH、LH、E2已趋于稳定,无很大波动;而卵泡直径在降调节15 d,4.5～7.0 mm的卵泡所占比例较10 d组增加,继续降调节至18 d,该组卵泡数并不增多,但3～4 mm的小卵泡数有所增加。因此,适当延长GnRH-a降调节时间可以改善卵泡发育的同步性。展开更多
文摘Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian development red swamp crayfish （Procambarus clarkii）. The results showed that all the three differ- ent methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree, with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in order immersion method 〉 injection method 〉 topical apphcation method 〉 control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish, with the order of immersion method 32.5%, injection method 20%, topical application 17.5% and the control group 17.5 %. Taking the synchro- nization spawning rate, final spawning rate and mortality into account, the order was the immersion method 〉 injection method 〉 topical application method 〉 control group.
基金Supported by the China National Science and Technology Major Project(2016ZX05050)
文摘It is difficult to build an effective water flooding displacement pressure system in the middle section of a horizontal well in an ultra-low permeability sandstone reservoir.To solve this problem,this study proposes to use packers,sealing cannula and other tools in the same horizontal well to inject water in some fractures and produce oil from other fractures.This new energy supplement method forms a segmental synchronous injection-production system in a horizontal well.The method can reduce the distance between the injection end and the production end,and quickly establish an effective displacement system.Changing the displacement between wells to displacement between horizontal well sections,and point water flooding to linear uniform water flooding,the method can enhance water sweeping volume and shorten waterflooding response period.The research shows that:(1)In the synchronous injection and production of horizontal well in an ultra-low-permeability sandstone reservoir,the water injection section should select the section where the natural fractures and artificial fractures are in the same direction or the section with no natural fractures,and the space between two sections should be 60?80 m.(2)In addition to controlling injection pressure,periodic water injection can be taken to reduce the risk of re-opening and growth of natural fractures or formation fracture caused by the gradual increase of water injection pressure with water injection going on.(3)Field tests have verified that this method can effectively improve the output of single well and achieve good economic benefits,so it can be widely used in the development of ultra-low permeability sandstone reservoirs.
文摘目的:通过比较绒毛膜促性腺激素释放激素激动剂(GnRH-a)降调节时间的不同对卵泡直径、子宫内膜厚度以及血卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)水平的影响,从而寻求体外受精-胚胎移植(IVF-ET)超促排卵相对合适的启动时间。方法:选择2009年在我中心进行IVF-ET的患者42例,全部采用黄体中期GnRH-a降调节的长方案。①根据GnRH-a降调节时间的不同分为降调节10、15、18 d 3组,分别进行B超监测卵泡的直径、子宫内膜厚度,抽血测定FSH、LH、E2水平,比较降调节时间的不同对卵泡直径、内膜厚度、性激素水平的影响。②降调节后的1、7、10、14 d,分别比较注射GnRH-a前以及注射后2、3 h血FSH、LH的变化。结果:①降调节10、15、18 d不同直径卵泡所占百分比:3～4 mm:16.8%vs 7.09%vs 10.38%;4.5～7.0 mm:80.24%vs89.55%vs 84.62;7.5～10 mm:2.96%vs 3.36%vs 5%。3～4 mm直径的卵泡在降调节10 d所占比例,与15、18 d有显著差异(P<0.05);4.5～7 mm直径的卵泡在15 d所占比例与10、18 d有显著差异(P<0.05);②降调节10、15、18 d内膜厚度(mm):7.73±2.48 vs 5.41±0.79 vs 5.24±0.85,降调节10 d内膜明显厚于15、18 d,有显著差异(P<0.05);③降调节10、15、18 d血FSH(mIU/ml):3.70±1.10 vs 3.51±0.72 vs 3.47±0.61;血LH(mIU/ml):1.23±1 vs 1.09±0.47 vs 1.22±0.72;E2:41.84±36.81 vs 32.84±14.32 vs 9.50±8.23,无显著差异。研究2:降调节后的1、7、10、14 d,注射GnRH-a后2、3 h,血FSH、LH都有升高,其中D1 d升高幅度最大,1.87±1.49 vs 13.33±7.81;1.06±1.13 vs 47.40±29.97,有统计学差异(P<0.05)。结论:在IVF-ET超促排卵长方案中,当GnRH-a降调节10 d,子宫内膜厚度、血FSH、LH、E2已趋于稳定,无很大波动;而卵泡直径在降调节15 d,4.5～7.0 mm的卵泡所占比例较10 d组增加,继续降调节至18 d,该组卵泡数并不增多,但3～4 mm的小卵泡数有所增加。因此,适当延长GnRH-a降调节时间可以改善卵泡发育的同步性。
