The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green ...The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green curves.The figure in the printed version has been corrected.The correct Figure 4 is shown below.The authors would like to apologize for any inconvenience caused.展开更多
The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinfor...The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinformatics, Issue 1, 2015. In the table, the image in the 4th row originally appeared in the article ＂Nanopore-based sequence-specific detection of duplex DNA for geno- mic profiling＂ published by Nano Letters in 2010. Therefore, Ref. [8], which was associated with the image in the current Table l, should be replaced by the reference： Singer A, Wanunu M, Morrison W, Kuhn H, Frank-Kamenetskii M, Meller A. Nanopore- based sequence specific detection of duplex DNA for genomic profiling. Nano Lett 2010; 10：738-42.＂ The copy right permission of this article has been obtained. The authors would like to apologize for any inconvenience caused.展开更多
本文介绍了第三代DNA测序的技术原理及应用现状,并对相关的生物信息学技术进行了综述。第三代测序技术以单分子测序为主要特点,目前已广泛应用于食品科学及生命科学研究的各个领域,其代表有Heliscope Bio Science公司的SMS技术、Pacific...本文介绍了第三代DNA测序的技术原理及应用现状,并对相关的生物信息学技术进行了综述。第三代测序技术以单分子测序为主要特点,目前已广泛应用于食品科学及生命科学研究的各个领域,其代表有Heliscope Bio Science公司的SMS技术、Pacific Bio Sciences公司的SMRT技术等。本文同时归纳总结了基因组学相关的生物信息学发展状况及常用的数据库。展开更多
为建立用于二代测序技术的最适细菌DNA提取方法。分别用磁珠法、热裂解法、试剂盒法(有溶菌酶处理和无溶菌酶处理)、超声波法、超声+热裂解法共6种方法提取等量混合的细菌DNA,特异性扩增其16S r RNA的V3-V4高变区基因,经二代测序并分析...为建立用于二代测序技术的最适细菌DNA提取方法。分别用磁珠法、热裂解法、试剂盒法(有溶菌酶处理和无溶菌酶处理)、超声波法、超声+热裂解法共6种方法提取等量混合的细菌DNA,特异性扩增其16S r RNA的V3-V4高变区基因,经二代测序并分析结果中的细菌种类及其相对含量分布。6种方法的测序结果中,细菌种类鉴定结果无明显差异;而在磁珠法和试剂盒法(有溶菌酶处理)结果中,不同细菌的含量分布最为接近样品中的真实情况。在6种方法中,磁珠法和试剂盒法(有溶菌酶处理)适合于二代测序技术中混合细菌DNA的提取。展开更多
文摘The authors regret that there were typos in the online version of Figure 4 published in Issue 1,2015.In the figure legend boxes of panels F and G,‘‘Ni’’should be corrected to‘‘N’’for the labeling of the green curves.The figure in the printed version has been corrected.The correct Figure 4 is shown below.The authors would like to apologize for any inconvenience caused.
文摘The authors regret that there was an incorrect citation in the Table 1 of the article ＂Nanopore-based Fourth-generation DNA Sequencing Technology＂, which was published in the journal Genomics, Proteomics ＆ Bioinformatics, Issue 1, 2015. In the table, the image in the 4th row originally appeared in the article ＂Nanopore-based sequence-specific detection of duplex DNA for geno- mic profiling＂ published by Nano Letters in 2010. Therefore, Ref. [8], which was associated with the image in the current Table l, should be replaced by the reference： Singer A, Wanunu M, Morrison W, Kuhn H, Frank-Kamenetskii M, Meller A. Nanopore- based sequence specific detection of duplex DNA for genomic profiling. Nano Lett 2010; 10：738-42.＂ The copy right permission of this article has been obtained. The authors would like to apologize for any inconvenience caused.
文摘本文介绍了第三代DNA测序的技术原理及应用现状,并对相关的生物信息学技术进行了综述。第三代测序技术以单分子测序为主要特点,目前已广泛应用于食品科学及生命科学研究的各个领域,其代表有Heliscope Bio Science公司的SMS技术、Pacific Bio Sciences公司的SMRT技术等。本文同时归纳总结了基因组学相关的生物信息学发展状况及常用的数据库。
文摘为建立用于二代测序技术的最适细菌DNA提取方法。分别用磁珠法、热裂解法、试剂盒法(有溶菌酶处理和无溶菌酶处理)、超声波法、超声+热裂解法共6种方法提取等量混合的细菌DNA,特异性扩增其16S r RNA的V3-V4高变区基因,经二代测序并分析结果中的细菌种类及其相对含量分布。6种方法的测序结果中,细菌种类鉴定结果无明显差异;而在磁珠法和试剂盒法(有溶菌酶处理)结果中,不同细菌的含量分布最为接近样品中的真实情况。在6种方法中,磁珠法和试剂盒法(有溶菌酶处理)适合于二代测序技术中混合细菌DNA的提取。
