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Establishment of Indirect ELISA Method for Detection of Penton Protein of Fowl Adenovirus GroupⅠ 被引量:1
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作者 LUO Si-si XIE Zhi-xun +5 位作者 XIE Zhi-qin LIU Jia-bo PANG Yao-shan DENG Xian-wen XIE Li-ji FAN Qing 《Animal Husbandry and Feed Science》 CAS 2012年第6期250-253,257,共5页
[ Objective] To establish an indirect ELISA method which can detect fowl adenovirus group I (FAVI) antibody easily and rapidly. [ Method] The expressed and purified FAVI penton recombinant protein was used to be an ... [ Objective] To establish an indirect ELISA method which can detect fowl adenovirus group I (FAVI) antibody easily and rapidly. [ Method] The expressed and purified FAVI penton recombinant protein was used to be an antigen, optimized the reaction conditions, and then estab- lished the FAVI indirect penton-ELISA antibody detection method. [ Result] The optimal coating concentration of antigen was 1.5 μg/hole, the opti- mal coating condition was 37℃ 2 h and 4 ℃ overnight; the optimal dilution of serum was 1:100; the optimal working concentration of anti-chicken IgG-HRP was 1:2 000; the positive and negative critical value of ELISA was 0.335. Detected the 100 chicken serum samples by the established penton-ELISA method, the positive rate was 41%. [ Conclusion] Through the study, ~e established penton-ELISA method has a good specificity, sensitivity and reproducibility. And it offers an effective tool for the diagnosis of FAVI, the survey of antibody and epidemiology survey. 展开更多
关键词 fowl adenovirus group i PENTON ELiSA
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Isolation and Characterization of A Novel Fowl Adenovirus Serotype 8a Strain from China 被引量:7
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作者 Li Chen Lijuan Yin +5 位作者 Peng Peng Qingfeng Zhou Yunping Du Yun Zhang Chunyi Xue Yongchang Cao 《Virologica Sinica》 SCIE CAS CSCD 2020年第5期517-527,共11页
Since 2012,the clinical cases of inclusion body hepatitis showed an increasing trend in China,causing considerable economic losses to the poultry industry.In this study,a fowl adenovirus strain CH/GDLZ/201801 was isol... Since 2012,the clinical cases of inclusion body hepatitis showed an increasing trend in China,causing considerable economic losses to the poultry industry.In this study,a fowl adenovirus strain CH/GDLZ/201801 was isolated from a chicken flock experiencing inclusion body hepatitis and analyzed by complete genome sequencing.The pathogenicity of the new virus strain was examined by experimental infection of specific pathogen free chickens.The isolate was identified by immunofluorescence and the virions presented typical icosahedral particles under transmission electron microscopy.The full genome of the isolate was 44,329 nucleotides in length with 58%G+C content.Phylogenetic analysis,based on the whole genome,revealed that the new isolate was closest to serotype 8a from the species Fowl aviadenovirus E(FAdVE).Recombination analysis and phylogenetic analysis showed that the new isolate is a recombinant strain between FAdV-8a and FAdV-8b.In infection experiments,three infected chickens showed clinical signs and one chicken died on day 7 post infection,corresponding to 5%mortality.Macroscopic and microscopic lesions in the liver were observed,and viral antigen could be detected in the livers by immunohistochemical staining and TEM.Taken together,our study describes the genomic characteristics and pathogenicity of a FAdV-8a strain in China.It would lay a solid foundation for further study of the pathogenic mechanism and vaccine development of the virus. 展开更多
关键词 fowl adenovirus serotype 8a(FAdV-8a) GENOME Phylogenetic analysis PATHOGENiCiTY
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Pathogenicity of an FAdV-4 isolate to chickens and its genomic analysis 被引量:11
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作者 Kai-kun MO Chen-fei LYU +6 位作者 Shang-shang CAO Xia LI Gang XING Yan YAN Xiao-juan ZHENG Min LIAO Ji-yong ZHOU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2019年第9期740-755,共16页
Fowl adenovirus serotype 4(FAdV-4)strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province,China.The isolate was cultured in primary chicken embr... Fowl adenovirus serotype 4(FAdV-4)strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province,China.The isolate was cultured in primary chicken embryo kidney cells.A study of pathogenicity indicated that SD1511 readily infected 7–35-d-old chickens by intramuscular injection and intranasal and oral routes,causing 50%–100%mortality.The 35-d-old chickens suffered more severe infection than 7-and 21-d-old chickens with mortality highest in the intramuscular injection group.The serum from surviving chickens showed potent viral neutralizing capability.The complete genome of SD1511 was sequenced and analyzed.The strain was found to belong to the FAdV-4 cluster with more than 99%identity with the virulent FAdV-4 strains isolated in China in recent years except for some distinct variations,including deletions of open reading frame 27(ORF27),ORF48,and part of ORF19.Our findings suggest that SD1511 might be used as a prototype strain for the study of pathogenesis and vaccine development. 展开更多
关键词 fowl adenovirus serotype 4(FAdV-4) PATHOGENiCiTY infection way Virus neutralization activity Genome analysis
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