The stray crystals seriously affect the properties of metals which fabricated by directional solidification(DS). In order to observe the formation of the stray crystals, the growth behavior of Sn-10 wt.%Bi alloy was o...The stray crystals seriously affect the properties of metals which fabricated by directional solidification(DS). In order to observe the formation of the stray crystals, the growth behavior of Sn-10 wt.%Bi alloy was observed by synchrotron X-ray imaging technique using self-developed directional solidification(DS) apparatus. The experimental results show that Sn–10 wt.%Bi alloy is oriented with the form of dendrites to grow into the liquid phase during the DS process, and dendrites are arranged neatly to form columnar crystal microstructures. It is also found that parts of the dendrite arms begin to crack. These cracked dendrites drift upward to grow up in front of the solid-liquid interface and form stray crystals.展开更多
An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linke...An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linked to this assay is licensed and often located within larger diagnostic centers.In this study,we present a protocol for using other types of software than SCSASoftR to determine the SDF index(DFI)with clinical relevance.This protocol is engineered after collect!ng and analyzing 254 samples from fertility patients and sperm donors over a 15-month period.DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid(pH 1.2)followed by acridine orange.DFI is determined by a standard flow cytometric software,FACSDiva 6.1.3.Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations(IUI)or timed coitus(TC).The results show that the chance of pregnancy decli nes as DFI in creases.We also found that the male DFI affects the chanee of pregnancy independent of the female age.We have shown that a standard flow cytometric software can be used when determi ning a clinical releva nt DFI.These findings are a sign ificant step toward impleme nting the an alysis as a part of the routi ne,in・house diag no sing of the male fertility patient and subseque ntly optimizing the treatme nt course of the couple with reduced human and financial costs.展开更多
By means of polym erase chain reaction- restriction fragment length polymorphism (PCR- RFL P) assay,the association between vitamine D receptor (VDR) genotypes and bone min- eral density (BMD) in the patients receiv...By means of polym erase chain reaction- restriction fragment length polymorphism (PCR- RFL P) assay,the association between vitamine D receptor (VDR) genotypes and bone min- eral density (BMD) in the patients receiving long- term glucocorticoid therapy was studied.The clinical data and blood of71patients with rheumatosis who received long- term glucocorticoid ther- apy were collected.BMD was m easured by dual- energy X- ray absorptimometry.VDR gene frag- ment(about185 bp) was amplified by PCR from the extracted genomic DNA,then digested with restriction endonuclease Bsm I.The genotypes were evaluated based on the fragment length fol- lowing endonuclease digestion and the association between genotypes and BMD or Z- score values was analyzed.Among the 71cases,the detected genotypes were Bb and bb with the distribution frequency being 11.3% and 88.7% respectively.The distribution frequency of the alleles was in agreement with the Hardy- Weinberg equilibrium.There was no significant difference between the two genotypes in age,gender,body m ass index(BMI) ,disease duration,disease types,time of glucocorticoid administration and cumulative dosage(P>0 .0 5 ) .Osteoporosis rate of the patients with Bb or bb genotype was37.5 % and33.3% respectively,with the difference being notsignif- icant (χ2 =0 .0 5 ,P=0 .8) .The BMD and Z- score values at lumbar spine and femur in two geno- types were not similar,but the difference had no significant (P>0 .0 5 ) .The distribution frequen- cy of bb type of VDR genotypes in Han populations of China was m ore prevalent,followed by Bb and bb types in turn.In the patients receiving long- term glucocorticoid therapy,there was no sig- nificant difference in BMD between Bb and bb genotypes.The data suggest that the VDR geno- types may not be m eans of identifying patients at greater risk of glucocorticoid- induced osteoporo- sis,which await to be further confirmed by a large sample size.展开更多
基金financially supported by the project of Yangtze Normal University under grant No.2017KYQD130 and No.2017XJQN08
文摘The stray crystals seriously affect the properties of metals which fabricated by directional solidification(DS). In order to observe the formation of the stray crystals, the growth behavior of Sn-10 wt.%Bi alloy was observed by synchrotron X-ray imaging technique using self-developed directional solidification(DS) apparatus. The experimental results show that Sn–10 wt.%Bi alloy is oriented with the form of dendrites to grow into the liquid phase during the DS process, and dendrites are arranged neatly to form columnar crystal microstructures. It is also found that parts of the dendrite arms begin to crack. These cracked dendrites drift upward to grow up in front of the solid-liquid interface and form stray crystals.
文摘An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linked to this assay is licensed and often located within larger diagnostic centers.In this study,we present a protocol for using other types of software than SCSASoftR to determine the SDF index(DFI)with clinical relevance.This protocol is engineered after collect!ng and analyzing 254 samples from fertility patients and sperm donors over a 15-month period.DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid(pH 1.2)followed by acridine orange.DFI is determined by a standard flow cytometric software,FACSDiva 6.1.3.Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations(IUI)or timed coitus(TC).The results show that the chance of pregnancy decli nes as DFI in creases.We also found that the male DFI affects the chanee of pregnancy independent of the female age.We have shown that a standard flow cytometric software can be used when determi ning a clinical releva nt DFI.These findings are a sign ificant step toward impleme nting the an alysis as a part of the routi ne,in・house diag no sing of the male fertility patient and subseque ntly optimizing the treatme nt course of the couple with reduced human and financial costs.
文摘By means of polym erase chain reaction- restriction fragment length polymorphism (PCR- RFL P) assay,the association between vitamine D receptor (VDR) genotypes and bone min- eral density (BMD) in the patients receiving long- term glucocorticoid therapy was studied.The clinical data and blood of71patients with rheumatosis who received long- term glucocorticoid ther- apy were collected.BMD was m easured by dual- energy X- ray absorptimometry.VDR gene frag- ment(about185 bp) was amplified by PCR from the extracted genomic DNA,then digested with restriction endonuclease Bsm I.The genotypes were evaluated based on the fragment length fol- lowing endonuclease digestion and the association between genotypes and BMD or Z- score values was analyzed.Among the 71cases,the detected genotypes were Bb and bb with the distribution frequency being 11.3% and 88.7% respectively.The distribution frequency of the alleles was in agreement with the Hardy- Weinberg equilibrium.There was no significant difference between the two genotypes in age,gender,body m ass index(BMI) ,disease duration,disease types,time of glucocorticoid administration and cumulative dosage(P>0 .0 5 ) .Osteoporosis rate of the patients with Bb or bb genotype was37.5 % and33.3% respectively,with the difference being notsignif- icant (χ2 =0 .0 5 ,P=0 .8) .The BMD and Z- score values at lumbar spine and femur in two geno- types were not similar,but the difference had no significant (P>0 .0 5 ) .The distribution frequen- cy of bb type of VDR genotypes in Han populations of China was m ore prevalent,followed by Bb and bb types in turn.In the patients receiving long- term glucocorticoid therapy,there was no sig- nificant difference in BMD between Bb and bb genotypes.The data suggest that the VDR geno- types may not be m eans of identifying patients at greater risk of glucocorticoid- induced osteoporo- sis,which await to be further confirmed by a large sample size.
