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一种无标记基因植物表达载体的改造方法 被引量:1
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作者 周红 姜良良 +1 位作者 燕飞 陈剑平 《浙江农业学报》 CSCD 北大核心 2013年第4期804-807,共4页
利用无标记基因表达载体开展转基因植物研究,是获得无标记基因转基因植物、保证转基因生物安全的手段之一。文章报道了一种无标记基因植物转化载体的改造方法,通过AseⅠ单酶切可去除pCAM-BIA1300植物转化载体内潮霉素标记基因完整表达... 利用无标记基因表达载体开展转基因植物研究,是获得无标记基因转基因植物、保证转基因生物安全的手段之一。文章报道了一种无标记基因植物转化载体的改造方法,通过AseⅠ单酶切可去除pCAM-BIA1300植物转化载体内潮霉素标记基因完整表达框架。试验应用结果表明,利用此表达载体进行共转化研究,可获得不含标记基因、只含目的基因的转基因植株。此方法可在植物共转化研究中广泛应用。 展开更多
关键词 无标记 载体 转基因 生物安全
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Research of testing method based on UML statecharts
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作者 占学德 《Journal of Shanghai University(English Edition)》 CAS 2006年第5期469-470,共2页
Unified modeling language (UML) is a powerful graphical modeling language with intuitional meaning. It provides various diagrams to depict system characteristics and complex environment from different viewpoints and... Unified modeling language (UML) is a powerful graphical modeling language with intuitional meaning. It provides various diagrams to depict system characteristics and complex environment from different viewpoints and different application layers. UML-based software development and modeling environments have been widely accepted in industry, including areas in which safety is an important issue such as spaceflight, defense, automobile, etc. To ensure and improve software quality becomes a main concern in the field. As one of the key techniques for software quality, software testing can effectively detect system faults. UML based software testing based is an important research direction in software engineering. The key to software testing is the generation of test cases. This dissertation studies an approach to generating test cases from UML statecharts. 展开更多
关键词 unified modeling language (UML) statechart formalsemantics flattened regular expression free model specification based software testing test criteria automatic generation of test case.
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