Effect of free-air CO_2 enrichment on nematode communities in a Chinese farmland ecosystem

At a rice wheat rotational free air CO 2 enrichment(FACE) platform, the effect of elevated atmospheric CO 2 on soil nematode communities in a farmland ecosystem was studied. Wheat plots were exposed to elevated atmospheric CO 2(ambient 370 μl/L + 200 μl/L). 32 families and 40 genera of nematode were observed in soil suspensions during the study period. Under FACE treatment, the numbers of total nematodes, bacterivores and fungivores exhibited an increasing trend. Because of the seasonal variation of soil temperature and moisture, the effect of elevated atmospheric CO 2 on soil nematodes was only observed under favorable conditions. The response of nematode communities to elevated atmospheric CO 2 may indicate the change of soil food web.

Effects of Free-air CO2 Enrichment on Root Characteristics and C:N Ratio of Rice at the Heading Stage

A hydroponics experiment was conducted to investigate the rice root growth in FACE （free-air carbon dioxide enrichment）. The root biomass, root volume, ratio of root/shoot, number of adventitious roots and root diameter significantly increased under FACE conditions, while the CO2 enrichment decreased the N concentration in rice roots without any change in the C content, leading to an increase in root C：N ratio. Moreover, the elevated CO2 resulted in a remarkable decrease of root activity, expressed as per unit root dry weight, which might be responsible for decreased N concentration in roots.

Effects of CO_2 enrichment and spikelet removal on rice quality under open-air field conditions

The increase of atmospheric carbon dioxide（CO_2） concentration adversely affect several quality traits of rice grains, but the biochemical mechanism remains unclear. The objectives of this study were to determine how changes in the source-sink relationship affected rice quality. Source-sink manipulation was achieved by free-air CO_2 enrichment from tillering to maturity and partial removal of spikelet at anthesis using a japonica rice cultivar Wuyunjing 23. Enrichment with CO_2 decreased the head rice percentage and protein concentration of milled rice, but increased the grain chalkiness. In contrast, spikelet removal resulted in a dramatic increase in the head rice percentage and protein concentration, and much less grain chalkiness. Neither CO_2 enrichment nor spikelet removal affected the starch content, but the distribution of starch granule size showed distinct treatment effects. O n average, spikelet removal decreased the percentage of starch granules of diameter 〉10 and 5–10 μm by 23.6 and 5.6%, respectively, and increased those with a diameter of 2–5 and 〈2 μm by 4.6 and 3.3%, respectively. In contrast, CO_2 elevation showed an opposite response： increasing the proportion of large starch granules（〉5 μm） and decreasing that of 〈5 μm. The starch pasting properties were affected by spikelet removal much more than by CO_2 elevation. These results indicated that the protein concentration and starch granule size played a role in chalkiness formation under these experimental conditions.

开放式空气中CO_2浓度增高(FACE)对水稻生长和发育的影响

人类活动导致的大气和气候变化将极大地改变作物的生长环境,其中最大的一个变化就是大气二氧化碳(CO2)浓度的迅速上升:从工业革命前的平均270μmol/mol上升到目前的381μmol/mol,到2050年至少超过550μmol/mol。FACE(Free-air CO2 enrichment,开放式空气中CO2浓度增高)试验是目前评估未来高浓度CO2对作物生长和产量实际影响的最佳方法。水稻无疑是人类最重要的食物来源,迄今为止人类利用FACE技术开展水稻响应和适应的研究已有10a(19982008年)的历史。以生长发育为主线,首次系统综述了10a水稻FACE试验在该领域的研究成果,总结了FACE情形下高浓度CO2(模拟本世纪中叶大气CO2浓度)对主要供试水稻品种(小区面积大于4m2)光合作用、生育进程、地上部生长、地下部生长、物质分配、籽粒灌浆、产量构成以及倒伏性状等影响的研究进展,比较了FACE与非FACE研究之间以及中国和日本FACE研究(世界上唯一的两个大型水稻FACE研究)之间的异同点。根据研究进展以及当前的技术水平,文章最后提出了该领域的3个优先课题:(1)FACE情形下杂交稻生产力响应高于预期的生物学机制;(2)FACE情形下CO2与主要栽培措施的互作效应;(3)FACE情形下CO2与主要空气污染物臭氧的互作效应。这些响应的机理性解析将有助于从根本上减少人类预测未来粮食安全的不确定性,进而更加有效地制订出应对全球变化的适应策略。

A possible mechanism of mineral responses to elevated atmospheric CO_2 in rice grains

Increasing attentions have been paid to mineral concentration decrease in milled rice grains caused by CO2 enrichment, but the mechanisms still remain unclear. Therefore, mineral （Ca, Mg, Fe, Zn and Mn） translocation in plant-soil system with a FACE （Free-air CO2 enrichment） experiment were investigated in Eastern China after 4-yr operation. Results mainly showed that： （1） elevated CO2 significantly increased the biomass of stem and panicle by 21.9 and 24.0%, respectively, but did not affect the leaf biomass. （2） Elevated CO2 significantly increased the contents of Ca, Mg, Fe, Zn, and Mn in panicle by 61.2, 28.9, 87.0, 36.7, and 66.0%, respectively, and in stem by 13.2, 21.3, 47.2, 91.8, and 25.2%, respectively, but did not affect them in leaf. （3） Elevated CO2 had positive effects on the weight ratio of mineral/biomass in stem and panicle. Our results suggest that elevated CO2 can favor the translocation of Ca, Mg, Fe, Zn, and Mn from soil to stem and panicle. The CO2-led mineral decline in milled rice grains may mainly attribute to the CO2-1ed unbalanced stimulations on the translocations of minerals and carbohydrates from vegetative parts （e.g., leaf, stem, branch and husk） to the grains.

Effects of free-air CO_(2) enrichment(FACE)and nitrogen(N)supply on N uptake and utilization of indica and japonica cultivars(Oryza sativa L.)

Background:Increasing atmospheric[CO_(2)]can increase photosynthesis and promote plant growth,consequently influencing nitrogen(N)cycling.Yet,there is no systematic information on the N response among different organs of japonica and indica rice genotypes to elevated[CO_(2)]as affected by N application.To investigate the impacts of elevated[CO_(2)]and N fertilization on N uptake and utilization of different genotypic rice(Oryza sativa L.)during grain filling,a free-air CO_(2) enrichment(FACE)experiment with indica cv.Liangyou 084(IIY084)and japonica cv.Wuyunjing 23(WYJ23)was conducted in Eastern China.Crops were exposed to ambient[CO_(2)]and elevated[CO_(2)](200μmol mol^(−1) above ambient)at two levels of N:control(0N)and 22.5 g N m^(−2)(normal N,NN),and they were sampled at 82 days after transplanting(DAT),99 DAT,and maturity,respectively.Results:Under FACE,significant declines of N concentration in all tissues and the whole plants were observed with the greater decrease in leaves and stems at three stages.Positive responses of N use efficiency(NUE)to elevated[CO_(2)]were recorded over the study period.The total N accumulation remained unchanged,while a large amount of N was partitioned to panicles at the expense of leaves and stems.As compared to WYJ23,greater N transportation from roots to aboveground,especially the panicles,was observed on IIY084 accompanied by higher panicle biomass(82 DAT and 99 DAT),N concentrations(maturity),and greater NUE for leaves through the study season.Across all[CO_(2)]and cultivars,N fertilization increased N partitioning to leaves and stems while decreasing that to panicles.Additionally,N supply decreased NUE while stimulating N concentrations and N amounts of rice plants.Among all treatments,IIY084 had the highest N accumulation and allocation in panicles under elevated[CO_(2)]in combination with N fertilizer at maturity.Conclusion:Data from this study were helpful for understanding the temporal N uptake and utilization of different rice genotypes as affected by N availability and suggest that IIY084 promises a considerable prospect for its grain yield and quality under future elevated atmospheric[CO_(2)].

CO_(2)浓度升高条件下稗草与水稻生长空间竞争关系研究

为明确CO_(2)浓度升高条件下稗草(Echinochloa crusgalli)与水稻(Oryza sativa)生长空间的竞争关系,以“吉粳88”为研究对象,利用开放式CO_(2)富集系统(Free-air CO_(2) enrichment,FACE),开展了水稻与稗草共生混种试验。试验设置2个处理,CO_(2)摩尔分数分别为400μmol·mol^(−1)(自然大气情景下)和550μmol·mol^(−1)(高浓度CO_(2)环境应用FACE系统进行调控)。每个CO_(2)浓度处理中设2个稗草密度,分别为水稻清种和水稻与稗草混种,共计4个组合。以自然大气CO_(2)浓度下水稻清种处理为对照(CK),自然大气CO_(2)浓度下水稻与稗草混种处理为TB、高浓度CO_(2)下水稻清种处理为TC、高浓度CO_(2)下水稻与稗草混种为BC。在水稻各发育期分别测定形态生理指标和根系指标并进行分析。结果表明,稗草混种处理对水稻每穴穗数和结实率均有负向作用,但未达到显著水平。稗草对水稻拔节期的株高有负向作用,稗草混种处理对水稻分蘖期叶面积指数下降了23.2%,使水稻在抽穗期分蘖数减少了34.5%,均达到显著水平。稗草混种处理降低了水稻根系氧化力,在水稻分蘖期、拔节期、抽穗期分别降低了41.6%、10.9%、14.2%,且均达到显著水平。稗草混种处理对水稻根系总吸收面积有负向作用,且在抽穗期和成熟期达到显著水平,在分蘖期达到极显著水平,CO_(2)浓度升高与稗草互作对水稻根系总吸收面积影响以负效应为主。研究结果明确了在CO_(2)浓度升高的条件下,稗草对水稻分蘖期和拔节期生长空间表现出明显的抑制作用,也是稗草与水稻生长竞争的关键时期。

Elevated CO_(2) increases soil redox potential by promoting root radial oxygen loss in paddy field

Soil redox potential(Eh)plays an important role in the biogeochemical cycling of soil nutrients.Whereas its effect soil process and nutrients'availability under elevated atmospheric CO_(2) concentration and warming has seldom been investigated.Thus,in this study,a field experiment was used to elucidate the effect of elevated CO_(2) concentration and warming on soil Eh,redox-sensitive elements and root radial oxygen loss(ROL).We hypothesized elevated CO_(2) and warming could alter soil Eh by promoting or inhibiting ROL.We found that soil Eh in the rhizosphere was significantly higher than that of non-rhizosphere.Elevated CO_(2) enhanced soil Eh by 11.5%,which corresponded to a significant decrease in soil Fe^(2+)and Mn^(2+)concentration.Under elevated CO_(2),the concentration of Fe^(2+)and Mn^(2+)decreased by 14.7%and 13.7%,respectively.We also found that elevated CO_(2) altered rice root aerenchyma structure and promoted rice root ROL.Under elevated CO_(2),rice root ROL increased by 79.5%and 112.2%for Yangdao 6 and Changyou 5,respectively.Warming had no effect on soil Eh and rice root ROL.While warming increased the concentration of Mn^(2+)and SO_(4)^(2-)by 4.9%and 19.3%,respectively.There was a significant interaction between elevated CO_(2) and warming on Fe^(2+)and Mn^(2+).Under elevated CO_(2),warming had no effect on the concentration of Fe^(2+)but decreased Mn^(2+)concentration significantly.Our study demonstrated that elevated atmospheric CO_(2) in the future could increase soil Eh by promoting rice root ROL,which will alter some soil nutrients'availability,such as Fe^(2+)and Mn^(2+).

Elevated atmospheric CO_(2) reduces CH_(4)and N_(2)O emissions under two contrasting rice cultivars from a subtropical paddy field in China

Elevated CO_(2)(eCO_(2))and rice cultivars can strongly alter CH_(4)and N_(2)O emissions from paddy fields.However,detailed information on how their interaction affects greenhouse gas fluxes in the field is still lacking.In this study,we investigated CH_(4)and N_(2)O emissions and rice growth under two contrasting rice cultivars(the strongly and weakly responsive cultivars)in response to eCO_(2),200μmol mol^(-1)higher than the ambient CO_(2)(aCO_(2)),in Chinese subtropical rice systems relying on a multi-year in-situ free-air CO_(2)enrichment platform from 2016 to 2018.The results showed that compared to aCO_(2),eCO_(2)increased rice yield by 7%-31%,while it decreased seasonal cumulative CH_(4)and N_(2)O emissions by 10%-59%and 33%-70%,respectively,regardless of rice cultivar.The decrease in CH_(4) emissions under eCO_(2)was possibly ascribed to the lower CH_(4)production potential(MPP)and the higher CH_(4)oxidation potential(MOP)correlated with the higher soil redox potential(Eh)and O_(2)concentration([O_(2)])in the surface soil.The mitigating effect of eCO_(2)on N_(2)O emissions was likely associated with the reduction of soil soluble N content.The strongly responsive cultivars had lower CH_(4)and N_(2)O emissions than the weakly re sponsive cultivars,and the main reason might be that the former induced higher soil Eh and[O_(2)]in the surface soil and had larger plant biomass and greater N uptake.The findings indicated that breeding strongly responsive cultivars with the potential for greater rice production and lower greenhouse gas emissions is an effective agricultural practice to ensure food security and environmental sustainability under future climate change scenarios.

Modeling Methane Emissions from Paddy Rice Fields under Elevated Atmospheric Carbon Dioxide Conditions

Abstract Methane （CH4） emissions from paddy rice fields substantially contribute to the dramatic increase of this greenhouse gas in the atmosphere. Due to great concern about climate change, it is necessary to predict the effects of the dramatic increase in atmospheric carbon dioxide （CO2） on CH4 emissions from paddy rice fields. CH4MOD 1.0 is the most widely validated model for simulating CH4 emissions from paddy rice fields exposed to ambient CO2 （hereinafter referred to as aCO2）. We upgraded the model to CH4MOD 2.0 by： （a） modifying the description of the influences of soil Eh and the water regime on CH4 production; （b） adding new features to reflect the regulatory effects of atmospheric CO2 upon methanogenic substrates, soil Eh during drainages, and vascular CH4 transport; and （c） adding a new feature to simulate the influences of nitrogen （N） addition rates on methanogenic substrates under elevated CO2 （hereinafter referred to as eCO2） condition. Validation with 109 observation cases under aC02 condition showed that CHaMOD 2.0 possessed a minor systematic bias in the prediction of seasonally accumulated methane emissions （SAM）. Validation with observations in free-air CO2 enrichment （FACE） experiments in temperate and subtropical climates showed that CH4MOD 2.0 successfully simulated the effects of eCO2 upon SAM from paddy rice fields incorporated with various levels of previous crop residues and/or N fertilizer. Our results imply that CH4MOD 2.0 provides a potential approach for estimating of the effects of elevated atmospheric CO2 upon CHa emissions from regional or global paddy rice fields with various management practices in a changing climate.

Elevated CO_2 facilitates C and N accumulation in a rice paddy ecosystem

Elevated CO2 can stimulate wetland carbon （C） and nitrogen （N） exports through gaseous and dissolved pathways, however, the consequent influences on the C and N pools are still not fully known. Therefore, we set up a free-air CO2 enrichment experiment in a paddy field in Eastern China. After five year fumigation, we studied C and N in the plant-water-soil system. The results showed： （1） elevated CO2 stimulated rice aboveground biomass and N accumulations by 19.1% and 12.5%, respectively. （2） Elevated CO2 significantly increased paddy soil TOC and TN contents by 12.5% and 15.5%, respectively in the 0-15 crn layer, and 22.7% and 26.0% in the 15-30 cm soil layer. （3） Averaged across the rice growing period, elevated CO2 greatly increased TOC and TN contents in the surface water by 7.6% and 11.4%, respectively. （4） The TOC/TN ratio and natural 5ISN value in the surface soil showed a decreasing trend under elevated CO2. The above results indicate that elevated CO2 can benefit C and N accumulation in paddy fields. Given the similarity between the paddies and natural wetlands, our results also suggest a great potential for long-term C and N accumulation in natural wetlands under future climate patterns.

高浓度CO2对稻穗不同位置籽粒结实和米质性状的影响

不断升高的大气CO2浓度影响水稻颖花发育、灌浆结实和品质形成,但这种影响是否与籽粒在稻穗上的着生部位有关尚不清楚.利用稻田FACE(Free-Air CO2 Enrichment)平台,以优质丰产粳稻‘武运粳23’为材料,CO2处理设背景CO2浓度(Ambient)和高CO2浓度(增200μmol·mol^-1,FACE)两个水平,研究开放大田条件下高浓度CO2对水稻颖花密度、籽粒结实能力、稻米外观和食味品质的影响及其与稻穗不同着生位置的关系.结果表明:FACE处理使武运粳23籽粒产量平均增加18.3%,从产量构成因素看,穗数和饱粒重分别增加21.4%、9.4%,每穗颖花数、饱粒率平均减少9.0%、2.2%.FACE水稻饱粒率下降主要与稻穗不同部位空粒率大幅增加有关.FACE水稻每穗颖花数减少主要与稻穗上部、中部二次枝梗现存颖花大幅减少有关,而其他位置颖花数均无显著变化;稻穗不同位置饱粒重和饱粒率对FACE的响应无显著差异.FACE处理使绿粒率下降,但糙米长度和宽度均增加,稻穗不同部位趋势一致.FACE使垩白粒率(增幅59%)、垩白度(增幅55%)均极显著增加,增幅表现为稻穗一次枝梗>二次枝梗、上部>中部>下部.FACE使稻穗不同位置稻米直链淀粉含量略增,使最高粘度、热浆粘度、崩解值、最终粘度和消减值略降,但多未达显著水平.FACE使稻米糊化温度显著下降,弱势粒的降幅大于强势粒.综上,高浓度CO2环境下武运粳23产量增加主要与穗数增多和籽粒增重有关,而稻穗明显变小;高浓度CO2使稻米绿粒率减少,垩白增多,而对蒸煮食味品质影响较少;颖花着生位置对高浓度CO2环境下水稻颖花发育、结实和品质的影响因不同测定指标而异.

Effect of Elevated CO2 on the Growth and Macronutrient （N, P and K） Uptake of Annual Wormwood （Artemisia annua L.）

Annual wormwood(Artemisia annua L.) is the only viable source of artemisinin,an antimalarial drug.There is a pressing need to optimize production per cultivated area of this important medicinal plant;however,the effect of increasing atmospheric carbon dioxide(CO_2) concentration on its growth is still unclear.Therefore,a pot experiment was conducted in a free-air CO2 enrichment(FACE) facility in Yangzhou City,China.Two A.annua varieties,one wild and one cultivated,were grown under ambient(374μmol mol^(-1)) and elevated(577 μmol mol^(-1)) CO_2 levels to determine the dry matter accumulation and macronutrient uptake of aerial parts.The results showed that stem and leaf yields of both A.annua varieties increased significantly under elevated CO_2 due to the enhanced photosynthesis rate.Although nitrogen(N),phosphorus(P),and potassium(K) concentrations in leaves and stems of both varieties decreased under elevated CO_2,total shoot N,P,and K uptake of the two varieties were enhanced and the ratios among the concentrations of these nutrients(N:P,N:K,and P:K) were not affected by elevated CO_2.Overall,our results provided the evidence that elevated CO_2 increased biomass and shoot macronutrient uptake of two A.annua varieties.